Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide
- Autores
- Gudesblat, G.E.; Iusem, N.D.; Morris, P.C.
- Año de publicación
- 2007
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- MAP kinases have been linked to guard cell signalling. Arabidopsis thaliana MAP Kinase 3 (MPK3) is known to be activated by abscisic acid (ABA) and hydrogen peroxide (H2O2), which also control stomatal movements. We therefore studied the possible role of MPK3 in guard cell signalling through guard cell-specific antisense inhibition of MPK3 expression. Such transgenic plants contained reduced levels of MPK3 mRNA in the guard cells and displayed partial insensitivity to ABA in inhibition of stomatal opening, but responded normally to this hormone in stomatal closure. However, ABA-induced stomatal closure was reduced compared with controls when cytoplasmic alkalinization was prevented with sodium butyrate. MPK3 antisense plants were less sensitive to exogenous H2O2, both in inhibition of stomatal opening and in promotion of stomatal closure, thus MPK3 is required for the signalling of this compound. ABA-induced H2O2 synthesis was normal in these plants, indicating that MPK3 probably acts in signalling downstream of H2O2. These results provide clear evidence for the important role of MPK3 in the perception of ABA and H 2O2 in guard cells. © The Authors (2007).
Fil:Gudesblat, G.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Iusem, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. - Fuente
- New Phytol. 2007;173(4):713-721
- Materia
-
Abscisic acid (ABA)
Arabidopsis thaliana
Hydrogen peroxide (H 2O2)
MAP kinase
MPK3
Stomata
abscisic acid
Arabidopsis protein
complementary DNA
hydrogen peroxide
mitogen activated protein kinase 3
abnormality
abscisic acid
alkalinization
enzyme activity
gene expression
hydrogen peroxide
inhibition
RNA
signal
stomatal conductance
transgenic plant
Arabidopsis
article
cytology
genetic transformation
genetics
metabolism
physiology
plant epidermis
plant leaf
signal transduction
transgenic plant
Abscisic Acid
Arabidopsis
Arabidopsis Proteins
DNA, Antisense
Hydrogen Peroxide
Mitogen-Activated Protein Kinase 3
Plant Epidermis
Plant Leaves
Plants, Genetically Modified
Signal Transduction
Transformation, Genetic
Arabidopsis
Arabidopsis thaliana - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/2.5/ar
- Repositorio
- Institución
- Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
- OAI Identificador
- paperaa:paper_0028646X_v173_n4_p713_Gudesblat
Ver los metadatos del registro completo
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paperaa:paper_0028646X_v173_n4_p713_Gudesblat |
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network_name_str |
Biblioteca Digital (UBA-FCEN) |
spelling |
Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxideGudesblat, G.E.Iusem, N.D.Morris, P.C.Abscisic acid (ABA)Arabidopsis thalianaHydrogen peroxide (H 2O2)MAP kinaseMPK3Stomataabscisic acidArabidopsis proteincomplementary DNAhydrogen peroxidemitogen activated protein kinase 3abnormalityabscisic acidalkalinizationenzyme activitygene expressionhydrogen peroxideinhibitionRNAsignalstomatal conductancetransgenic plantArabidopsisarticlecytologygenetic transformationgeneticsmetabolismphysiologyplant epidermisplant leafsignal transductiontransgenic plantAbscisic AcidArabidopsisArabidopsis ProteinsDNA, AntisenseHydrogen PeroxideMitogen-Activated Protein Kinase 3Plant EpidermisPlant LeavesPlants, Genetically ModifiedSignal TransductionTransformation, GeneticArabidopsisArabidopsis thalianaMAP kinases have been linked to guard cell signalling. Arabidopsis thaliana MAP Kinase 3 (MPK3) is known to be activated by abscisic acid (ABA) and hydrogen peroxide (H2O2), which also control stomatal movements. We therefore studied the possible role of MPK3 in guard cell signalling through guard cell-specific antisense inhibition of MPK3 expression. Such transgenic plants contained reduced levels of MPK3 mRNA in the guard cells and displayed partial insensitivity to ABA in inhibition of stomatal opening, but responded normally to this hormone in stomatal closure. However, ABA-induced stomatal closure was reduced compared with controls when cytoplasmic alkalinization was prevented with sodium butyrate. MPK3 antisense plants were less sensitive to exogenous H2O2, both in inhibition of stomatal opening and in promotion of stomatal closure, thus MPK3 is required for the signalling of this compound. ABA-induced H2O2 synthesis was normal in these plants, indicating that MPK3 probably acts in signalling downstream of H2O2. These results provide clear evidence for the important role of MPK3 in the perception of ABA and H 2O2 in guard cells. © The Authors (2007).Fil:Gudesblat, G.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Iusem, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2007info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_0028646X_v173_n4_p713_GudesblatNew Phytol. 2007;173(4):713-721reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:42:54Zpaperaa:paper_0028646X_v173_n4_p713_GudesblatInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:42:55.784Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse |
dc.title.none.fl_str_mv |
Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide |
title |
Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide |
spellingShingle |
Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide Gudesblat, G.E. Abscisic acid (ABA) Arabidopsis thaliana Hydrogen peroxide (H 2O2) MAP kinase MPK3 Stomata abscisic acid Arabidopsis protein complementary DNA hydrogen peroxide mitogen activated protein kinase 3 abnormality abscisic acid alkalinization enzyme activity gene expression hydrogen peroxide inhibition RNA signal stomatal conductance transgenic plant Arabidopsis article cytology genetic transformation genetics metabolism physiology plant epidermis plant leaf signal transduction transgenic plant Abscisic Acid Arabidopsis Arabidopsis Proteins DNA, Antisense Hydrogen Peroxide Mitogen-Activated Protein Kinase 3 Plant Epidermis Plant Leaves Plants, Genetically Modified Signal Transduction Transformation, Genetic Arabidopsis Arabidopsis thaliana |
title_short |
Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide |
title_full |
Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide |
title_fullStr |
Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide |
title_full_unstemmed |
Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide |
title_sort |
Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide |
dc.creator.none.fl_str_mv |
Gudesblat, G.E. Iusem, N.D. Morris, P.C. |
author |
Gudesblat, G.E. |
author_facet |
Gudesblat, G.E. Iusem, N.D. Morris, P.C. |
author_role |
author |
author2 |
Iusem, N.D. Morris, P.C. |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Abscisic acid (ABA) Arabidopsis thaliana Hydrogen peroxide (H 2O2) MAP kinase MPK3 Stomata abscisic acid Arabidopsis protein complementary DNA hydrogen peroxide mitogen activated protein kinase 3 abnormality abscisic acid alkalinization enzyme activity gene expression hydrogen peroxide inhibition RNA signal stomatal conductance transgenic plant Arabidopsis article cytology genetic transformation genetics metabolism physiology plant epidermis plant leaf signal transduction transgenic plant Abscisic Acid Arabidopsis Arabidopsis Proteins DNA, Antisense Hydrogen Peroxide Mitogen-Activated Protein Kinase 3 Plant Epidermis Plant Leaves Plants, Genetically Modified Signal Transduction Transformation, Genetic Arabidopsis Arabidopsis thaliana |
topic |
Abscisic acid (ABA) Arabidopsis thaliana Hydrogen peroxide (H 2O2) MAP kinase MPK3 Stomata abscisic acid Arabidopsis protein complementary DNA hydrogen peroxide mitogen activated protein kinase 3 abnormality abscisic acid alkalinization enzyme activity gene expression hydrogen peroxide inhibition RNA signal stomatal conductance transgenic plant Arabidopsis article cytology genetic transformation genetics metabolism physiology plant epidermis plant leaf signal transduction transgenic plant Abscisic Acid Arabidopsis Arabidopsis Proteins DNA, Antisense Hydrogen Peroxide Mitogen-Activated Protein Kinase 3 Plant Epidermis Plant Leaves Plants, Genetically Modified Signal Transduction Transformation, Genetic Arabidopsis Arabidopsis thaliana |
dc.description.none.fl_txt_mv |
MAP kinases have been linked to guard cell signalling. Arabidopsis thaliana MAP Kinase 3 (MPK3) is known to be activated by abscisic acid (ABA) and hydrogen peroxide (H2O2), which also control stomatal movements. We therefore studied the possible role of MPK3 in guard cell signalling through guard cell-specific antisense inhibition of MPK3 expression. Such transgenic plants contained reduced levels of MPK3 mRNA in the guard cells and displayed partial insensitivity to ABA in inhibition of stomatal opening, but responded normally to this hormone in stomatal closure. However, ABA-induced stomatal closure was reduced compared with controls when cytoplasmic alkalinization was prevented with sodium butyrate. MPK3 antisense plants were less sensitive to exogenous H2O2, both in inhibition of stomatal opening and in promotion of stomatal closure, thus MPK3 is required for the signalling of this compound. ABA-induced H2O2 synthesis was normal in these plants, indicating that MPK3 probably acts in signalling downstream of H2O2. These results provide clear evidence for the important role of MPK3 in the perception of ABA and H 2O2 in guard cells. © The Authors (2007). Fil:Gudesblat, G.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Iusem, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. |
description |
MAP kinases have been linked to guard cell signalling. Arabidopsis thaliana MAP Kinase 3 (MPK3) is known to be activated by abscisic acid (ABA) and hydrogen peroxide (H2O2), which also control stomatal movements. We therefore studied the possible role of MPK3 in guard cell signalling through guard cell-specific antisense inhibition of MPK3 expression. Such transgenic plants contained reduced levels of MPK3 mRNA in the guard cells and displayed partial insensitivity to ABA in inhibition of stomatal opening, but responded normally to this hormone in stomatal closure. However, ABA-induced stomatal closure was reduced compared with controls when cytoplasmic alkalinization was prevented with sodium butyrate. MPK3 antisense plants were less sensitive to exogenous H2O2, both in inhibition of stomatal opening and in promotion of stomatal closure, thus MPK3 is required for the signalling of this compound. ABA-induced H2O2 synthesis was normal in these plants, indicating that MPK3 probably acts in signalling downstream of H2O2. These results provide clear evidence for the important role of MPK3 in the perception of ABA and H 2O2 in guard cells. © The Authors (2007). |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12110/paper_0028646X_v173_n4_p713_Gudesblat |
url |
http://hdl.handle.net/20.500.12110/paper_0028646X_v173_n4_p713_Gudesblat |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/2.5/ar |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
New Phytol. 2007;173(4):713-721 reponame:Biblioteca Digital (UBA-FCEN) instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales instacron:UBA-FCEN |
reponame_str |
Biblioteca Digital (UBA-FCEN) |
collection |
Biblioteca Digital (UBA-FCEN) |
instname_str |
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
instacron_str |
UBA-FCEN |
institution |
UBA-FCEN |
repository.name.fl_str_mv |
Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
repository.mail.fl_str_mv |
ana@bl.fcen.uba.ar |
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1844618734616969216 |
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13.070432 |