Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide

Autores
Gudesblat, G.E.; Iusem, N.D.; Morris, P.C.
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
MAP kinases have been linked to guard cell signalling. Arabidopsis thaliana MAP Kinase 3 (MPK3) is known to be activated by abscisic acid (ABA) and hydrogen peroxide (H2O2), which also control stomatal movements. We therefore studied the possible role of MPK3 in guard cell signalling through guard cell-specific antisense inhibition of MPK3 expression. Such transgenic plants contained reduced levels of MPK3 mRNA in the guard cells and displayed partial insensitivity to ABA in inhibition of stomatal opening, but responded normally to this hormone in stomatal closure. However, ABA-induced stomatal closure was reduced compared with controls when cytoplasmic alkalinization was prevented with sodium butyrate. MPK3 antisense plants were less sensitive to exogenous H2O2, both in inhibition of stomatal opening and in promotion of stomatal closure, thus MPK3 is required for the signalling of this compound. ABA-induced H2O2 synthesis was normal in these plants, indicating that MPK3 probably acts in signalling downstream of H2O2. These results provide clear evidence for the important role of MPK3 in the perception of ABA and H 2O2 in guard cells. © The Authors (2007).
Fil:Gudesblat, G.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Iusem, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
New Phytol. 2007;173(4):713-721
Materia
Abscisic acid (ABA)
Arabidopsis thaliana
Hydrogen peroxide (H 2O2)
MAP kinase
MPK3
Stomata
abscisic acid
Arabidopsis protein
complementary DNA
hydrogen peroxide
mitogen activated protein kinase 3
abnormality
abscisic acid
alkalinization
enzyme activity
gene expression
hydrogen peroxide
inhibition
RNA
signal
stomatal conductance
transgenic plant
Arabidopsis
article
cytology
genetic transformation
genetics
metabolism
physiology
plant epidermis
plant leaf
signal transduction
transgenic plant
Abscisic Acid
Arabidopsis
Arabidopsis Proteins
DNA, Antisense
Hydrogen Peroxide
Mitogen-Activated Protein Kinase 3
Plant Epidermis
Plant Leaves
Plants, Genetically Modified
Signal Transduction
Transformation, Genetic
Arabidopsis
Arabidopsis thaliana
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_0028646X_v173_n4_p713_Gudesblat

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oai_identifier_str paperaa:paper_0028646X_v173_n4_p713_Gudesblat
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxideGudesblat, G.E.Iusem, N.D.Morris, P.C.Abscisic acid (ABA)Arabidopsis thalianaHydrogen peroxide (H 2O2)MAP kinaseMPK3Stomataabscisic acidArabidopsis proteincomplementary DNAhydrogen peroxidemitogen activated protein kinase 3abnormalityabscisic acidalkalinizationenzyme activitygene expressionhydrogen peroxideinhibitionRNAsignalstomatal conductancetransgenic plantArabidopsisarticlecytologygenetic transformationgeneticsmetabolismphysiologyplant epidermisplant leafsignal transductiontransgenic plantAbscisic AcidArabidopsisArabidopsis ProteinsDNA, AntisenseHydrogen PeroxideMitogen-Activated Protein Kinase 3Plant EpidermisPlant LeavesPlants, Genetically ModifiedSignal TransductionTransformation, GeneticArabidopsisArabidopsis thalianaMAP kinases have been linked to guard cell signalling. Arabidopsis thaliana MAP Kinase 3 (MPK3) is known to be activated by abscisic acid (ABA) and hydrogen peroxide (H2O2), which also control stomatal movements. We therefore studied the possible role of MPK3 in guard cell signalling through guard cell-specific antisense inhibition of MPK3 expression. Such transgenic plants contained reduced levels of MPK3 mRNA in the guard cells and displayed partial insensitivity to ABA in inhibition of stomatal opening, but responded normally to this hormone in stomatal closure. However, ABA-induced stomatal closure was reduced compared with controls when cytoplasmic alkalinization was prevented with sodium butyrate. MPK3 antisense plants were less sensitive to exogenous H2O2, both in inhibition of stomatal opening and in promotion of stomatal closure, thus MPK3 is required for the signalling of this compound. ABA-induced H2O2 synthesis was normal in these plants, indicating that MPK3 probably acts in signalling downstream of H2O2. These results provide clear evidence for the important role of MPK3 in the perception of ABA and H 2O2 in guard cells. © The Authors (2007).Fil:Gudesblat, G.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Iusem, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2007info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_0028646X_v173_n4_p713_GudesblatNew Phytol. 2007;173(4):713-721reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:42:54Zpaperaa:paper_0028646X_v173_n4_p713_GudesblatInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:42:55.784Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide
title Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide
spellingShingle Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide
Gudesblat, G.E.
Abscisic acid (ABA)
Arabidopsis thaliana
Hydrogen peroxide (H 2O2)
MAP kinase
MPK3
Stomata
abscisic acid
Arabidopsis protein
complementary DNA
hydrogen peroxide
mitogen activated protein kinase 3
abnormality
abscisic acid
alkalinization
enzyme activity
gene expression
hydrogen peroxide
inhibition
RNA
signal
stomatal conductance
transgenic plant
Arabidopsis
article
cytology
genetic transformation
genetics
metabolism
physiology
plant epidermis
plant leaf
signal transduction
transgenic plant
Abscisic Acid
Arabidopsis
Arabidopsis Proteins
DNA, Antisense
Hydrogen Peroxide
Mitogen-Activated Protein Kinase 3
Plant Epidermis
Plant Leaves
Plants, Genetically Modified
Signal Transduction
Transformation, Genetic
Arabidopsis
Arabidopsis thaliana
title_short Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide
title_full Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide
title_fullStr Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide
title_full_unstemmed Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide
title_sort Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide
dc.creator.none.fl_str_mv Gudesblat, G.E.
Iusem, N.D.
Morris, P.C.
author Gudesblat, G.E.
author_facet Gudesblat, G.E.
Iusem, N.D.
Morris, P.C.
author_role author
author2 Iusem, N.D.
Morris, P.C.
author2_role author
author
dc.subject.none.fl_str_mv Abscisic acid (ABA)
Arabidopsis thaliana
Hydrogen peroxide (H 2O2)
MAP kinase
MPK3
Stomata
abscisic acid
Arabidopsis protein
complementary DNA
hydrogen peroxide
mitogen activated protein kinase 3
abnormality
abscisic acid
alkalinization
enzyme activity
gene expression
hydrogen peroxide
inhibition
RNA
signal
stomatal conductance
transgenic plant
Arabidopsis
article
cytology
genetic transformation
genetics
metabolism
physiology
plant epidermis
plant leaf
signal transduction
transgenic plant
Abscisic Acid
Arabidopsis
Arabidopsis Proteins
DNA, Antisense
Hydrogen Peroxide
Mitogen-Activated Protein Kinase 3
Plant Epidermis
Plant Leaves
Plants, Genetically Modified
Signal Transduction
Transformation, Genetic
Arabidopsis
Arabidopsis thaliana
topic Abscisic acid (ABA)
Arabidopsis thaliana
Hydrogen peroxide (H 2O2)
MAP kinase
MPK3
Stomata
abscisic acid
Arabidopsis protein
complementary DNA
hydrogen peroxide
mitogen activated protein kinase 3
abnormality
abscisic acid
alkalinization
enzyme activity
gene expression
hydrogen peroxide
inhibition
RNA
signal
stomatal conductance
transgenic plant
Arabidopsis
article
cytology
genetic transformation
genetics
metabolism
physiology
plant epidermis
plant leaf
signal transduction
transgenic plant
Abscisic Acid
Arabidopsis
Arabidopsis Proteins
DNA, Antisense
Hydrogen Peroxide
Mitogen-Activated Protein Kinase 3
Plant Epidermis
Plant Leaves
Plants, Genetically Modified
Signal Transduction
Transformation, Genetic
Arabidopsis
Arabidopsis thaliana
dc.description.none.fl_txt_mv MAP kinases have been linked to guard cell signalling. Arabidopsis thaliana MAP Kinase 3 (MPK3) is known to be activated by abscisic acid (ABA) and hydrogen peroxide (H2O2), which also control stomatal movements. We therefore studied the possible role of MPK3 in guard cell signalling through guard cell-specific antisense inhibition of MPK3 expression. Such transgenic plants contained reduced levels of MPK3 mRNA in the guard cells and displayed partial insensitivity to ABA in inhibition of stomatal opening, but responded normally to this hormone in stomatal closure. However, ABA-induced stomatal closure was reduced compared with controls when cytoplasmic alkalinization was prevented with sodium butyrate. MPK3 antisense plants were less sensitive to exogenous H2O2, both in inhibition of stomatal opening and in promotion of stomatal closure, thus MPK3 is required for the signalling of this compound. ABA-induced H2O2 synthesis was normal in these plants, indicating that MPK3 probably acts in signalling downstream of H2O2. These results provide clear evidence for the important role of MPK3 in the perception of ABA and H 2O2 in guard cells. © The Authors (2007).
Fil:Gudesblat, G.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Iusem, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description MAP kinases have been linked to guard cell signalling. Arabidopsis thaliana MAP Kinase 3 (MPK3) is known to be activated by abscisic acid (ABA) and hydrogen peroxide (H2O2), which also control stomatal movements. We therefore studied the possible role of MPK3 in guard cell signalling through guard cell-specific antisense inhibition of MPK3 expression. Such transgenic plants contained reduced levels of MPK3 mRNA in the guard cells and displayed partial insensitivity to ABA in inhibition of stomatal opening, but responded normally to this hormone in stomatal closure. However, ABA-induced stomatal closure was reduced compared with controls when cytoplasmic alkalinization was prevented with sodium butyrate. MPK3 antisense plants were less sensitive to exogenous H2O2, both in inhibition of stomatal opening and in promotion of stomatal closure, thus MPK3 is required for the signalling of this compound. ABA-induced H2O2 synthesis was normal in these plants, indicating that MPK3 probably acts in signalling downstream of H2O2. These results provide clear evidence for the important role of MPK3 in the perception of ABA and H 2O2 in guard cells. © The Authors (2007).
publishDate 2007
dc.date.none.fl_str_mv 2007
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_0028646X_v173_n4_p713_Gudesblat
url http://hdl.handle.net/20.500.12110/paper_0028646X_v173_n4_p713_Gudesblat
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv New Phytol. 2007;173(4):713-721
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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