Trypanosoma cruzi surface mucins with exposed variant epitopes
- Autores
- Pollevick, G.D.; Di Noia, J.M.; Salto, M.L.; Lima, C.; Leguizamón, M.S.; De Lederkremer, R.M.; Frasch, A.C.C.
- Año de publicación
- 2000
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The protozoan parasite Trypanosoma cruzi, the agent of Chagas disease, has a large number of mucin molecules on its surface, whose expression is regulated during the life cycle. These mucins are the main acceptors of sialic acid, a monosaccharide that is required by the parasite to infect and survive in the mammalian host. A large mucin-like gene family named TcMUC containing about 500 members has been identified previously in T. cruzi. TcMUC can be divided into two subfamilies according to the presence or absence of tandem repeats in the central region of the genes. In this work, T. cruzi parasites were transfected with one tagged member of each subfamily. Only the product from the gene with repeats was highly O-glycosylated in vivo. The O-linked oligosaccharides consisted mainly of β-D-Galp(1→4)-GlcNAc and β-D-Galp(1→4)[β-D-Galp(1→6)]-D-GlcNAc. The same glycosyl moieties were found in endogenous mucins. The mature product was anchored by glycosylphosphatidylinositol to the plasma membrane and exposed to the medium. Sera from infected mice recognized the recombinant product of one repeats-containing gene thus showing that they are expressed during the infection. TcMUC genes encode a hypervariable region at the N terminus. We now show that the hypervariable region is indeed present in the exposed mature N termini of the mucins because sera from infected hosts recognized peptides having sequences from this region. The results are discussed in comparison with the mucins from the insect stages of the parasite (Di Noia, J. M., D'Orso, I., Sanchez, D. O., and Frasch, A. C. C. (2000) J. Biol. Chem. 275, 10218-10227) which do not have variable regions.
Fil:Pollevick, G.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Di Noia, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Salto, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Leguizamón, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:De Lederkremer, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. - Fuente
- J. Biol. Chem. 2000;275(36):27671-27680
- Materia
-
epitope
gene product
glycosylphosphatidylinositol
monosaccharide
mucin
sialic acid
amino terminal sequence
animal cell
article
cell interaction
cell membrane
Chagas disease
gene expression
gene sequence
genetic transfection
mammal
multigene family
nonhuman
polyacrylamide gel electrophoresis
priority journal
protein expression
tandem repeat
Trypanosoma cruzi
vertebrate
Amino Acid Sequence
Animals
Antigens, Protozoan
Base Sequence
Carbohydrate Sequence
Chagas Disease
Epitopes
Glycosylation
Glycosylphosphatidylinositols
Mice
Molecular Sequence Data
Mucins
Oligosaccharides
Recombinant Proteins
Transfection
Trypanosoma cruzi
Variation (Genetics)
Animalia
Insecta
Mammalia
Protozoa
Trypanosoma
Trypanosoma cruzi
Vertebrata - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/2.5/ar
- Repositorio
- Institución
- Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
- OAI Identificador
- paperaa:paper_00219258_v275_n36_p27671_Pollevick
Ver los metadatos del registro completo
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spelling |
Trypanosoma cruzi surface mucins with exposed variant epitopesPollevick, G.D.Di Noia, J.M.Salto, M.L.Lima, C.Leguizamón, M.S.De Lederkremer, R.M.Frasch, A.C.C.epitopegene productglycosylphosphatidylinositolmonosaccharidemucinsialic acidamino terminal sequenceanimal cellarticlecell interactioncell membraneChagas diseasegene expressiongene sequencegenetic transfectionmammalmultigene familynonhumanpolyacrylamide gel electrophoresispriority journalprotein expressiontandem repeatTrypanosoma cruzivertebrateAmino Acid SequenceAnimalsAntigens, ProtozoanBase SequenceCarbohydrate SequenceChagas DiseaseEpitopesGlycosylationGlycosylphosphatidylinositolsMiceMolecular Sequence DataMucinsOligosaccharidesRecombinant ProteinsTransfectionTrypanosoma cruziVariation (Genetics)AnimaliaInsectaMammaliaProtozoaTrypanosomaTrypanosoma cruziVertebrataThe protozoan parasite Trypanosoma cruzi, the agent of Chagas disease, has a large number of mucin molecules on its surface, whose expression is regulated during the life cycle. These mucins are the main acceptors of sialic acid, a monosaccharide that is required by the parasite to infect and survive in the mammalian host. A large mucin-like gene family named TcMUC containing about 500 members has been identified previously in T. cruzi. TcMUC can be divided into two subfamilies according to the presence or absence of tandem repeats in the central region of the genes. In this work, T. cruzi parasites were transfected with one tagged member of each subfamily. Only the product from the gene with repeats was highly O-glycosylated in vivo. The O-linked oligosaccharides consisted mainly of β-D-Galp(1→4)-GlcNAc and β-D-Galp(1→4)[β-D-Galp(1→6)]-D-GlcNAc. The same glycosyl moieties were found in endogenous mucins. The mature product was anchored by glycosylphosphatidylinositol to the plasma membrane and exposed to the medium. Sera from infected mice recognized the recombinant product of one repeats-containing gene thus showing that they are expressed during the infection. TcMUC genes encode a hypervariable region at the N terminus. We now show that the hypervariable region is indeed present in the exposed mature N termini of the mucins because sera from infected hosts recognized peptides having sequences from this region. The results are discussed in comparison with the mucins from the insect stages of the parasite (Di Noia, J. M., D'Orso, I., Sanchez, D. O., and Frasch, A. C. C. (2000) J. Biol. Chem. 275, 10218-10227) which do not have variable regions.Fil:Pollevick, G.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Di Noia, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Salto, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Leguizamón, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:De Lederkremer, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2000info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00219258_v275_n36_p27671_PollevickJ. Biol. Chem. 2000;275(36):27671-27680reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:43:06Zpaperaa:paper_00219258_v275_n36_p27671_PollevickInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:43:07.29Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse |
dc.title.none.fl_str_mv |
Trypanosoma cruzi surface mucins with exposed variant epitopes |
title |
Trypanosoma cruzi surface mucins with exposed variant epitopes |
spellingShingle |
Trypanosoma cruzi surface mucins with exposed variant epitopes Pollevick, G.D. epitope gene product glycosylphosphatidylinositol monosaccharide mucin sialic acid amino terminal sequence animal cell article cell interaction cell membrane Chagas disease gene expression gene sequence genetic transfection mammal multigene family nonhuman polyacrylamide gel electrophoresis priority journal protein expression tandem repeat Trypanosoma cruzi vertebrate Amino Acid Sequence Animals Antigens, Protozoan Base Sequence Carbohydrate Sequence Chagas Disease Epitopes Glycosylation Glycosylphosphatidylinositols Mice Molecular Sequence Data Mucins Oligosaccharides Recombinant Proteins Transfection Trypanosoma cruzi Variation (Genetics) Animalia Insecta Mammalia Protozoa Trypanosoma Trypanosoma cruzi Vertebrata |
title_short |
Trypanosoma cruzi surface mucins with exposed variant epitopes |
title_full |
Trypanosoma cruzi surface mucins with exposed variant epitopes |
title_fullStr |
Trypanosoma cruzi surface mucins with exposed variant epitopes |
title_full_unstemmed |
Trypanosoma cruzi surface mucins with exposed variant epitopes |
title_sort |
Trypanosoma cruzi surface mucins with exposed variant epitopes |
dc.creator.none.fl_str_mv |
Pollevick, G.D. Di Noia, J.M. Salto, M.L. Lima, C. Leguizamón, M.S. De Lederkremer, R.M. Frasch, A.C.C. |
author |
Pollevick, G.D. |
author_facet |
Pollevick, G.D. Di Noia, J.M. Salto, M.L. Lima, C. Leguizamón, M.S. De Lederkremer, R.M. Frasch, A.C.C. |
author_role |
author |
author2 |
Di Noia, J.M. Salto, M.L. Lima, C. Leguizamón, M.S. De Lederkremer, R.M. Frasch, A.C.C. |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
epitope gene product glycosylphosphatidylinositol monosaccharide mucin sialic acid amino terminal sequence animal cell article cell interaction cell membrane Chagas disease gene expression gene sequence genetic transfection mammal multigene family nonhuman polyacrylamide gel electrophoresis priority journal protein expression tandem repeat Trypanosoma cruzi vertebrate Amino Acid Sequence Animals Antigens, Protozoan Base Sequence Carbohydrate Sequence Chagas Disease Epitopes Glycosylation Glycosylphosphatidylinositols Mice Molecular Sequence Data Mucins Oligosaccharides Recombinant Proteins Transfection Trypanosoma cruzi Variation (Genetics) Animalia Insecta Mammalia Protozoa Trypanosoma Trypanosoma cruzi Vertebrata |
topic |
epitope gene product glycosylphosphatidylinositol monosaccharide mucin sialic acid amino terminal sequence animal cell article cell interaction cell membrane Chagas disease gene expression gene sequence genetic transfection mammal multigene family nonhuman polyacrylamide gel electrophoresis priority journal protein expression tandem repeat Trypanosoma cruzi vertebrate Amino Acid Sequence Animals Antigens, Protozoan Base Sequence Carbohydrate Sequence Chagas Disease Epitopes Glycosylation Glycosylphosphatidylinositols Mice Molecular Sequence Data Mucins Oligosaccharides Recombinant Proteins Transfection Trypanosoma cruzi Variation (Genetics) Animalia Insecta Mammalia Protozoa Trypanosoma Trypanosoma cruzi Vertebrata |
dc.description.none.fl_txt_mv |
The protozoan parasite Trypanosoma cruzi, the agent of Chagas disease, has a large number of mucin molecules on its surface, whose expression is regulated during the life cycle. These mucins are the main acceptors of sialic acid, a monosaccharide that is required by the parasite to infect and survive in the mammalian host. A large mucin-like gene family named TcMUC containing about 500 members has been identified previously in T. cruzi. TcMUC can be divided into two subfamilies according to the presence or absence of tandem repeats in the central region of the genes. In this work, T. cruzi parasites were transfected with one tagged member of each subfamily. Only the product from the gene with repeats was highly O-glycosylated in vivo. The O-linked oligosaccharides consisted mainly of β-D-Galp(1→4)-GlcNAc and β-D-Galp(1→4)[β-D-Galp(1→6)]-D-GlcNAc. The same glycosyl moieties were found in endogenous mucins. The mature product was anchored by glycosylphosphatidylinositol to the plasma membrane and exposed to the medium. Sera from infected mice recognized the recombinant product of one repeats-containing gene thus showing that they are expressed during the infection. TcMUC genes encode a hypervariable region at the N terminus. We now show that the hypervariable region is indeed present in the exposed mature N termini of the mucins because sera from infected hosts recognized peptides having sequences from this region. The results are discussed in comparison with the mucins from the insect stages of the parasite (Di Noia, J. M., D'Orso, I., Sanchez, D. O., and Frasch, A. C. C. (2000) J. Biol. Chem. 275, 10218-10227) which do not have variable regions. Fil:Pollevick, G.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Di Noia, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Salto, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Leguizamón, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:De Lederkremer, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. |
description |
The protozoan parasite Trypanosoma cruzi, the agent of Chagas disease, has a large number of mucin molecules on its surface, whose expression is regulated during the life cycle. These mucins are the main acceptors of sialic acid, a monosaccharide that is required by the parasite to infect and survive in the mammalian host. A large mucin-like gene family named TcMUC containing about 500 members has been identified previously in T. cruzi. TcMUC can be divided into two subfamilies according to the presence or absence of tandem repeats in the central region of the genes. In this work, T. cruzi parasites were transfected with one tagged member of each subfamily. Only the product from the gene with repeats was highly O-glycosylated in vivo. The O-linked oligosaccharides consisted mainly of β-D-Galp(1→4)-GlcNAc and β-D-Galp(1→4)[β-D-Galp(1→6)]-D-GlcNAc. The same glycosyl moieties were found in endogenous mucins. The mature product was anchored by glycosylphosphatidylinositol to the plasma membrane and exposed to the medium. Sera from infected mice recognized the recombinant product of one repeats-containing gene thus showing that they are expressed during the infection. TcMUC genes encode a hypervariable region at the N terminus. We now show that the hypervariable region is indeed present in the exposed mature N termini of the mucins because sera from infected hosts recognized peptides having sequences from this region. The results are discussed in comparison with the mucins from the insect stages of the parasite (Di Noia, J. M., D'Orso, I., Sanchez, D. O., and Frasch, A. C. C. (2000) J. Biol. Chem. 275, 10218-10227) which do not have variable regions. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12110/paper_00219258_v275_n36_p27671_Pollevick |
url |
http://hdl.handle.net/20.500.12110/paper_00219258_v275_n36_p27671_Pollevick |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/2.5/ar |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
J. Biol. Chem. 2000;275(36):27671-27680 reponame:Biblioteca Digital (UBA-FCEN) instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales instacron:UBA-FCEN |
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Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
instacron_str |
UBA-FCEN |
institution |
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repository.name.fl_str_mv |
Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
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