Trypanosoma cruzi surface mucins with exposed variant epitopes

Autores
Pollevick, G.D.; Di Noia, J.M.; Salto, M.L.; Lima, C.; Leguizamón, M.S.; De Lederkremer, R.M.; Frasch, A.C.C.
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The protozoan parasite Trypanosoma cruzi, the agent of Chagas disease, has a large number of mucin molecules on its surface, whose expression is regulated during the life cycle. These mucins are the main acceptors of sialic acid, a monosaccharide that is required by the parasite to infect and survive in the mammalian host. A large mucin-like gene family named TcMUC containing about 500 members has been identified previously in T. cruzi. TcMUC can be divided into two subfamilies according to the presence or absence of tandem repeats in the central region of the genes. In this work, T. cruzi parasites were transfected with one tagged member of each subfamily. Only the product from the gene with repeats was highly O-glycosylated in vivo. The O-linked oligosaccharides consisted mainly of β-D-Galp(1→4)-GlcNAc and β-D-Galp(1→4)[β-D-Galp(1→6)]-D-GlcNAc. The same glycosyl moieties were found in endogenous mucins. The mature product was anchored by glycosylphosphatidylinositol to the plasma membrane and exposed to the medium. Sera from infected mice recognized the recombinant product of one repeats-containing gene thus showing that they are expressed during the infection. TcMUC genes encode a hypervariable region at the N terminus. We now show that the hypervariable region is indeed present in the exposed mature N termini of the mucins because sera from infected hosts recognized peptides having sequences from this region. The results are discussed in comparison with the mucins from the insect stages of the parasite (Di Noia, J. M., D'Orso, I., Sanchez, D. O., and Frasch, A. C. C. (2000) J. Biol. Chem. 275, 10218-10227) which do not have variable regions.
Fil:Pollevick, G.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Di Noia, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Salto, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Leguizamón, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:De Lederkremer, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
J. Biol. Chem. 2000;275(36):27671-27680
Materia
epitope
gene product
glycosylphosphatidylinositol
monosaccharide
mucin
sialic acid
amino terminal sequence
animal cell
article
cell interaction
cell membrane
Chagas disease
gene expression
gene sequence
genetic transfection
mammal
multigene family
nonhuman
polyacrylamide gel electrophoresis
priority journal
protein expression
tandem repeat
Trypanosoma cruzi
vertebrate
Amino Acid Sequence
Animals
Antigens, Protozoan
Base Sequence
Carbohydrate Sequence
Chagas Disease
Epitopes
Glycosylation
Glycosylphosphatidylinositols
Mice
Molecular Sequence Data
Mucins
Oligosaccharides
Recombinant Proteins
Transfection
Trypanosoma cruzi
Variation (Genetics)
Animalia
Insecta
Mammalia
Protozoa
Trypanosoma
Trypanosoma cruzi
Vertebrata
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_00219258_v275_n36_p27671_Pollevick

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oai_identifier_str paperaa:paper_00219258_v275_n36_p27671_Pollevick
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Trypanosoma cruzi surface mucins with exposed variant epitopesPollevick, G.D.Di Noia, J.M.Salto, M.L.Lima, C.Leguizamón, M.S.De Lederkremer, R.M.Frasch, A.C.C.epitopegene productglycosylphosphatidylinositolmonosaccharidemucinsialic acidamino terminal sequenceanimal cellarticlecell interactioncell membraneChagas diseasegene expressiongene sequencegenetic transfectionmammalmultigene familynonhumanpolyacrylamide gel electrophoresispriority journalprotein expressiontandem repeatTrypanosoma cruzivertebrateAmino Acid SequenceAnimalsAntigens, ProtozoanBase SequenceCarbohydrate SequenceChagas DiseaseEpitopesGlycosylationGlycosylphosphatidylinositolsMiceMolecular Sequence DataMucinsOligosaccharidesRecombinant ProteinsTransfectionTrypanosoma cruziVariation (Genetics)AnimaliaInsectaMammaliaProtozoaTrypanosomaTrypanosoma cruziVertebrataThe protozoan parasite Trypanosoma cruzi, the agent of Chagas disease, has a large number of mucin molecules on its surface, whose expression is regulated during the life cycle. These mucins are the main acceptors of sialic acid, a monosaccharide that is required by the parasite to infect and survive in the mammalian host. A large mucin-like gene family named TcMUC containing about 500 members has been identified previously in T. cruzi. TcMUC can be divided into two subfamilies according to the presence or absence of tandem repeats in the central region of the genes. In this work, T. cruzi parasites were transfected with one tagged member of each subfamily. Only the product from the gene with repeats was highly O-glycosylated in vivo. The O-linked oligosaccharides consisted mainly of β-D-Galp(1→4)-GlcNAc and β-D-Galp(1→4)[β-D-Galp(1→6)]-D-GlcNAc. The same glycosyl moieties were found in endogenous mucins. The mature product was anchored by glycosylphosphatidylinositol to the plasma membrane and exposed to the medium. Sera from infected mice recognized the recombinant product of one repeats-containing gene thus showing that they are expressed during the infection. TcMUC genes encode a hypervariable region at the N terminus. We now show that the hypervariable region is indeed present in the exposed mature N termini of the mucins because sera from infected hosts recognized peptides having sequences from this region. The results are discussed in comparison with the mucins from the insect stages of the parasite (Di Noia, J. M., D'Orso, I., Sanchez, D. O., and Frasch, A. C. C. (2000) J. Biol. Chem. 275, 10218-10227) which do not have variable regions.Fil:Pollevick, G.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Di Noia, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Salto, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Leguizamón, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:De Lederkremer, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2000info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00219258_v275_n36_p27671_PollevickJ. Biol. Chem. 2000;275(36):27671-27680reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:43:06Zpaperaa:paper_00219258_v275_n36_p27671_PollevickInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:43:07.29Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Trypanosoma cruzi surface mucins with exposed variant epitopes
title Trypanosoma cruzi surface mucins with exposed variant epitopes
spellingShingle Trypanosoma cruzi surface mucins with exposed variant epitopes
Pollevick, G.D.
epitope
gene product
glycosylphosphatidylinositol
monosaccharide
mucin
sialic acid
amino terminal sequence
animal cell
article
cell interaction
cell membrane
Chagas disease
gene expression
gene sequence
genetic transfection
mammal
multigene family
nonhuman
polyacrylamide gel electrophoresis
priority journal
protein expression
tandem repeat
Trypanosoma cruzi
vertebrate
Amino Acid Sequence
Animals
Antigens, Protozoan
Base Sequence
Carbohydrate Sequence
Chagas Disease
Epitopes
Glycosylation
Glycosylphosphatidylinositols
Mice
Molecular Sequence Data
Mucins
Oligosaccharides
Recombinant Proteins
Transfection
Trypanosoma cruzi
Variation (Genetics)
Animalia
Insecta
Mammalia
Protozoa
Trypanosoma
Trypanosoma cruzi
Vertebrata
title_short Trypanosoma cruzi surface mucins with exposed variant epitopes
title_full Trypanosoma cruzi surface mucins with exposed variant epitopes
title_fullStr Trypanosoma cruzi surface mucins with exposed variant epitopes
title_full_unstemmed Trypanosoma cruzi surface mucins with exposed variant epitopes
title_sort Trypanosoma cruzi surface mucins with exposed variant epitopes
dc.creator.none.fl_str_mv Pollevick, G.D.
Di Noia, J.M.
Salto, M.L.
Lima, C.
Leguizamón, M.S.
De Lederkremer, R.M.
Frasch, A.C.C.
author Pollevick, G.D.
author_facet Pollevick, G.D.
Di Noia, J.M.
Salto, M.L.
Lima, C.
Leguizamón, M.S.
De Lederkremer, R.M.
Frasch, A.C.C.
author_role author
author2 Di Noia, J.M.
Salto, M.L.
Lima, C.
Leguizamón, M.S.
De Lederkremer, R.M.
Frasch, A.C.C.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv epitope
gene product
glycosylphosphatidylinositol
monosaccharide
mucin
sialic acid
amino terminal sequence
animal cell
article
cell interaction
cell membrane
Chagas disease
gene expression
gene sequence
genetic transfection
mammal
multigene family
nonhuman
polyacrylamide gel electrophoresis
priority journal
protein expression
tandem repeat
Trypanosoma cruzi
vertebrate
Amino Acid Sequence
Animals
Antigens, Protozoan
Base Sequence
Carbohydrate Sequence
Chagas Disease
Epitopes
Glycosylation
Glycosylphosphatidylinositols
Mice
Molecular Sequence Data
Mucins
Oligosaccharides
Recombinant Proteins
Transfection
Trypanosoma cruzi
Variation (Genetics)
Animalia
Insecta
Mammalia
Protozoa
Trypanosoma
Trypanosoma cruzi
Vertebrata
topic epitope
gene product
glycosylphosphatidylinositol
monosaccharide
mucin
sialic acid
amino terminal sequence
animal cell
article
cell interaction
cell membrane
Chagas disease
gene expression
gene sequence
genetic transfection
mammal
multigene family
nonhuman
polyacrylamide gel electrophoresis
priority journal
protein expression
tandem repeat
Trypanosoma cruzi
vertebrate
Amino Acid Sequence
Animals
Antigens, Protozoan
Base Sequence
Carbohydrate Sequence
Chagas Disease
Epitopes
Glycosylation
Glycosylphosphatidylinositols
Mice
Molecular Sequence Data
Mucins
Oligosaccharides
Recombinant Proteins
Transfection
Trypanosoma cruzi
Variation (Genetics)
Animalia
Insecta
Mammalia
Protozoa
Trypanosoma
Trypanosoma cruzi
Vertebrata
dc.description.none.fl_txt_mv The protozoan parasite Trypanosoma cruzi, the agent of Chagas disease, has a large number of mucin molecules on its surface, whose expression is regulated during the life cycle. These mucins are the main acceptors of sialic acid, a monosaccharide that is required by the parasite to infect and survive in the mammalian host. A large mucin-like gene family named TcMUC containing about 500 members has been identified previously in T. cruzi. TcMUC can be divided into two subfamilies according to the presence or absence of tandem repeats in the central region of the genes. In this work, T. cruzi parasites were transfected with one tagged member of each subfamily. Only the product from the gene with repeats was highly O-glycosylated in vivo. The O-linked oligosaccharides consisted mainly of β-D-Galp(1→4)-GlcNAc and β-D-Galp(1→4)[β-D-Galp(1→6)]-D-GlcNAc. The same glycosyl moieties were found in endogenous mucins. The mature product was anchored by glycosylphosphatidylinositol to the plasma membrane and exposed to the medium. Sera from infected mice recognized the recombinant product of one repeats-containing gene thus showing that they are expressed during the infection. TcMUC genes encode a hypervariable region at the N terminus. We now show that the hypervariable region is indeed present in the exposed mature N termini of the mucins because sera from infected hosts recognized peptides having sequences from this region. The results are discussed in comparison with the mucins from the insect stages of the parasite (Di Noia, J. M., D'Orso, I., Sanchez, D. O., and Frasch, A. C. C. (2000) J. Biol. Chem. 275, 10218-10227) which do not have variable regions.
Fil:Pollevick, G.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Di Noia, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Salto, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Leguizamón, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:De Lederkremer, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description The protozoan parasite Trypanosoma cruzi, the agent of Chagas disease, has a large number of mucin molecules on its surface, whose expression is regulated during the life cycle. These mucins are the main acceptors of sialic acid, a monosaccharide that is required by the parasite to infect and survive in the mammalian host. A large mucin-like gene family named TcMUC containing about 500 members has been identified previously in T. cruzi. TcMUC can be divided into two subfamilies according to the presence or absence of tandem repeats in the central region of the genes. In this work, T. cruzi parasites were transfected with one tagged member of each subfamily. Only the product from the gene with repeats was highly O-glycosylated in vivo. The O-linked oligosaccharides consisted mainly of β-D-Galp(1→4)-GlcNAc and β-D-Galp(1→4)[β-D-Galp(1→6)]-D-GlcNAc. The same glycosyl moieties were found in endogenous mucins. The mature product was anchored by glycosylphosphatidylinositol to the plasma membrane and exposed to the medium. Sera from infected mice recognized the recombinant product of one repeats-containing gene thus showing that they are expressed during the infection. TcMUC genes encode a hypervariable region at the N terminus. We now show that the hypervariable region is indeed present in the exposed mature N termini of the mucins because sera from infected hosts recognized peptides having sequences from this region. The results are discussed in comparison with the mucins from the insect stages of the parasite (Di Noia, J. M., D'Orso, I., Sanchez, D. O., and Frasch, A. C. C. (2000) J. Biol. Chem. 275, 10218-10227) which do not have variable regions.
publishDate 2000
dc.date.none.fl_str_mv 2000
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_00219258_v275_n36_p27671_Pollevick
url http://hdl.handle.net/20.500.12110/paper_00219258_v275_n36_p27671_Pollevick
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv J. Biol. Chem. 2000;275(36):27671-27680
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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