Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8

• Autores: Pettinari, M.J.; Vázquez, G.J.; Silberschmidt, D.; Rehm, B.; Steibüchel, A.; Méndez, B.S.
• Año de publicación: 2001
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Appl. Environ. Microbiol. 2001;67(3-12):5331-5334
• Materia: acetoacetyl coenzyme a reductase; acetoacetyl-CoA reductase; acetyl coenzyme A acyltransferase; acyltransferase; alcohol dehydrogenase; hydroxybutyric acid; poly beta hydroxybutyrate polymerase; poly(3 hydroxybutyric acid); poly-beta-hydroxybutyrate; poly-beta-hydroxybutyrate polymerase; polyester; article; Azotobacter; bacterial gene; DNA sequence; gene deletion; genetics; growth, development and aging; metabolism; molecular cloning; molecular genetics; Acetyl-CoA C-Acyltransferase; Acyltransferases; Alcohol Oxidoreductases; Azotobacter; Cloning, Molecular; Gene Deletion; Genes, Bacterial; Hydroxybutyrates; Molecular Sequence Data; Polyesters; Sequence Analysis, DNA
• Institución: Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales

Genes responsible for the synthesis of poly(3-hydroxybutyrate) (PHB) in Azotobacter sp. FA8 were cloned and analyzed. A PHB polymerase gene (phbC) was found downstream from genes coding for β-ketothiolase (phbA) and acetoacetyl-coenzyme A reductase (phbB). A PHB synthase mutant was obtained by gene inactivation and used for genetic studies. The phbC gene from this strain was introduced into Raktonia eutropha PHB-4 (phbC-negative mutant), and the recombinant accumulated PHB when either glucose or oclanoate was used as a source of carbon, indicating that this PHB synthase cannot incorporate medium-chuin-length hydroxyalkanoates into PHB.
Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Vázquez, G.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Silberschmidt, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.