Impaired polyhydroxybutyrate biosynthesis from glucose in Pseudomonas sp. 14-3 is due to a defective β-ketothiolase gene

Autores
Ayub, N.D.; Julia Pettinari, M.; Méndez, B.S.; López, N.I.
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pseudomonas sp. 14-3 accumulates polyhydroxybutyrate (PHB) from octanoate, but not from glucose. To elucidate this unusual phenotype, genes responsible for the synthesis of PHB were cloned and analyzed. A PHB polymerase gene (phaC) was found downstream from genes coding for a β-ketothiolase (phaA), an acetoacetyl-coenzyme A reductase (phaB) and a putative transcriptional regulator (phaR). All genes were similar to pha genes from several related species, but differences were observed in the distal region of phaA. Complementation with heterologous β-ketothiolase genes from Azotobacter sp. FA8 or Pseudomonas putida GPp104 restored the capability of Pseudomonas sp. 14-3 to synthesize PHB from glucose, demonstrating that its β-ketothiolase was nonfunctional. Analysis of the genome sequences of other Pseudomonas species has revealed the existence of putative β-ketothiolase genes. The functionality of one of these thiolase genes, belonging to P. putida GPp104, was experimentally demonstrated. Pseudomonas sp. 14-3 is the first natural phaA mutant described, that despite this mutation accumulates high amounts of PHB when growing on fatty acids. © 2006 Federation of European Microbiological Societies.
Fil:Ayub, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Julia Pettinari, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
FEMS Microbiol. Lett. 2006;264(1):125-131
Materia
β-ketothiolase
Natural mutants
Polyhydroxybutyrate
Pseudomonas
acetyl coenzyme A acyltransferase
butyric acid derivative
polyhydroxybutyrate
unclassified drug
article
Azotobacter
biosynthesis
controlled study
gene expression
gene mutation
gene sequence
genetic analysis
molecular cloning
nonhuman
nucleotide sequence
phenotype
priority journal
Pseudomonas
Pseudomonas putida
transcription regulation
Acetyl-CoA C-Acyltransferase
Bacterial Proteins
Base Sequence
Cloning, Molecular
Genetic Complementation Test
Genome, Bacterial
Glucose
Hydroxybutyrates
Molecular Sequence Data
Octanoic Acids
Polyesters
Pseudomonas
Sequence Alignment
Azotobacter
Pseudomonas
Pseudomonas putida
Pseudomonas sp. 14-3
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_03781097_v264_n1_p125_Ayub
Acceder
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oai_identifier_str paperaa:paper_03781097_v264_n1_p125_Ayub
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Impaired polyhydroxybutyrate biosynthesis from glucose in Pseudomonas sp. 14-3 is due to a defective β-ketothiolase geneAyub, N.D.Julia Pettinari, M.Méndez, B.S.López, N.I.β-ketothiolaseNatural mutantsPolyhydroxybutyratePseudomonasacetyl coenzyme A acyltransferasebutyric acid derivativepolyhydroxybutyrateunclassified drugarticleAzotobacterbiosynthesiscontrolled studygene expressiongene mutationgene sequencegenetic analysismolecular cloningnonhumannucleotide sequencephenotypepriority journalPseudomonasPseudomonas putidatranscription regulationAcetyl-CoA C-AcyltransferaseBacterial ProteinsBase SequenceCloning, MolecularGenetic Complementation TestGenome, BacterialGlucoseHydroxybutyratesMolecular Sequence DataOctanoic AcidsPolyestersPseudomonasSequence AlignmentAzotobacterPseudomonasPseudomonas putidaPseudomonas sp. 14-3Pseudomonas sp. 14-3 accumulates polyhydroxybutyrate (PHB) from octanoate, but not from glucose. To elucidate this unusual phenotype, genes responsible for the synthesis of PHB were cloned and analyzed. A PHB polymerase gene (phaC) was found downstream from genes coding for a β-ketothiolase (phaA), an acetoacetyl-coenzyme A reductase (phaB) and a putative transcriptional regulator (phaR). All genes were similar to pha genes from several related species, but differences were observed in the distal region of phaA. Complementation with heterologous β-ketothiolase genes from Azotobacter sp. FA8 or Pseudomonas putida GPp104 restored the capability of Pseudomonas sp. 14-3 to synthesize PHB from glucose, demonstrating that its β-ketothiolase was nonfunctional. Analysis of the genome sequences of other Pseudomonas species has revealed the existence of putative β-ketothiolase genes. The functionality of one of these thiolase genes, belonging to P. putida GPp104, was experimentally demonstrated. Pseudomonas sp. 14-3 is the first natural phaA mutant described, that despite this mutation accumulates high amounts of PHB when growing on fatty acids. © 2006 Federation of European Microbiological Societies.Fil:Ayub, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Julia Pettinari, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2006info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_03781097_v264_n1_p125_AyubFEMS Microbiol. Lett. 2006;264(1):125-131reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-11T10:21:54Zpaperaa:paper_03781097_v264_n1_p125_AyubInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-11 10:21:55.656Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Impaired polyhydroxybutyrate biosynthesis from glucose in Pseudomonas sp. 14-3 is due to a defective β-ketothiolase gene
title Impaired polyhydroxybutyrate biosynthesis from glucose in Pseudomonas sp. 14-3 is due to a defective β-ketothiolase gene
spellingShingle Impaired polyhydroxybutyrate biosynthesis from glucose in Pseudomonas sp. 14-3 is due to a defective β-ketothiolase gene
Ayub, N.D.
β-ketothiolase
Natural mutants
Polyhydroxybutyrate
Pseudomonas
acetyl coenzyme A acyltransferase
butyric acid derivative
polyhydroxybutyrate
unclassified drug
article
Azotobacter
biosynthesis
controlled study
gene expression
gene mutation
gene sequence
genetic analysis
molecular cloning
nonhuman
nucleotide sequence
phenotype
priority journal
Pseudomonas
Pseudomonas putida
transcription regulation
Acetyl-CoA C-Acyltransferase
Bacterial Proteins
Base Sequence
Cloning, Molecular
Genetic Complementation Test
Genome, Bacterial
Glucose
Hydroxybutyrates
Molecular Sequence Data
Octanoic Acids
Polyesters
Pseudomonas
Sequence Alignment
Azotobacter
Pseudomonas
Pseudomonas putida
Pseudomonas sp. 14-3
title_short Impaired polyhydroxybutyrate biosynthesis from glucose in Pseudomonas sp. 14-3 is due to a defective β-ketothiolase gene
title_full Impaired polyhydroxybutyrate biosynthesis from glucose in Pseudomonas sp. 14-3 is due to a defective β-ketothiolase gene
title_fullStr Impaired polyhydroxybutyrate biosynthesis from glucose in Pseudomonas sp. 14-3 is due to a defective β-ketothiolase gene
title_full_unstemmed Impaired polyhydroxybutyrate biosynthesis from glucose in Pseudomonas sp. 14-3 is due to a defective β-ketothiolase gene
title_sort Impaired polyhydroxybutyrate biosynthesis from glucose in Pseudomonas sp. 14-3 is due to a defective β-ketothiolase gene
dc.creator.none.fl_str_mv Ayub, N.D.
Julia Pettinari, M.
Méndez, B.S.
López, N.I.
author Ayub, N.D.
author_facet Ayub, N.D.
Julia Pettinari, M.
Méndez, B.S.
López, N.I.
author_role author
author2 Julia Pettinari, M.
Méndez, B.S.
López, N.I.
author2_role author
author
author
dc.subject.none.fl_str_mv β-ketothiolase
Natural mutants
Polyhydroxybutyrate
Pseudomonas
acetyl coenzyme A acyltransferase
butyric acid derivative
polyhydroxybutyrate
unclassified drug
article
Azotobacter
biosynthesis
controlled study
gene expression
gene mutation
gene sequence
genetic analysis
molecular cloning
nonhuman
nucleotide sequence
phenotype
priority journal
Pseudomonas
Pseudomonas putida
transcription regulation
Acetyl-CoA C-Acyltransferase
Bacterial Proteins
Base Sequence
Cloning, Molecular
Genetic Complementation Test
Genome, Bacterial
Glucose
Hydroxybutyrates
Molecular Sequence Data
Octanoic Acids
Polyesters
Pseudomonas
Sequence Alignment
Azotobacter
Pseudomonas
Pseudomonas putida
Pseudomonas sp. 14-3
topic β-ketothiolase
Natural mutants
Polyhydroxybutyrate
Pseudomonas
acetyl coenzyme A acyltransferase
butyric acid derivative
polyhydroxybutyrate
unclassified drug
article
Azotobacter
biosynthesis
controlled study
gene expression
gene mutation
gene sequence
genetic analysis
molecular cloning
nonhuman
nucleotide sequence
phenotype
priority journal
Pseudomonas
Pseudomonas putida
transcription regulation
Acetyl-CoA C-Acyltransferase
Bacterial Proteins
Base Sequence
Cloning, Molecular
Genetic Complementation Test
Genome, Bacterial
Glucose
Hydroxybutyrates
Molecular Sequence Data
Octanoic Acids
Polyesters
Pseudomonas
Sequence Alignment
Azotobacter
Pseudomonas
Pseudomonas putida
Pseudomonas sp. 14-3
dc.description.none.fl_txt_mv Pseudomonas sp. 14-3 accumulates polyhydroxybutyrate (PHB) from octanoate, but not from glucose. To elucidate this unusual phenotype, genes responsible for the synthesis of PHB were cloned and analyzed. A PHB polymerase gene (phaC) was found downstream from genes coding for a β-ketothiolase (phaA), an acetoacetyl-coenzyme A reductase (phaB) and a putative transcriptional regulator (phaR). All genes were similar to pha genes from several related species, but differences were observed in the distal region of phaA. Complementation with heterologous β-ketothiolase genes from Azotobacter sp. FA8 or Pseudomonas putida GPp104 restored the capability of Pseudomonas sp. 14-3 to synthesize PHB from glucose, demonstrating that its β-ketothiolase was nonfunctional. Analysis of the genome sequences of other Pseudomonas species has revealed the existence of putative β-ketothiolase genes. The functionality of one of these thiolase genes, belonging to P. putida GPp104, was experimentally demonstrated. Pseudomonas sp. 14-3 is the first natural phaA mutant described, that despite this mutation accumulates high amounts of PHB when growing on fatty acids. © 2006 Federation of European Microbiological Societies.
Fil:Ayub, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Julia Pettinari, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description Pseudomonas sp. 14-3 accumulates polyhydroxybutyrate (PHB) from octanoate, but not from glucose. To elucidate this unusual phenotype, genes responsible for the synthesis of PHB were cloned and analyzed. A PHB polymerase gene (phaC) was found downstream from genes coding for a β-ketothiolase (phaA), an acetoacetyl-coenzyme A reductase (phaB) and a putative transcriptional regulator (phaR). All genes were similar to pha genes from several related species, but differences were observed in the distal region of phaA. Complementation with heterologous β-ketothiolase genes from Azotobacter sp. FA8 or Pseudomonas putida GPp104 restored the capability of Pseudomonas sp. 14-3 to synthesize PHB from glucose, demonstrating that its β-ketothiolase was nonfunctional. Analysis of the genome sequences of other Pseudomonas species has revealed the existence of putative β-ketothiolase genes. The functionality of one of these thiolase genes, belonging to P. putida GPp104, was experimentally demonstrated. Pseudomonas sp. 14-3 is the first natural phaA mutant described, that despite this mutation accumulates high amounts of PHB when growing on fatty acids. © 2006 Federation of European Microbiological Societies.
publishDate 2006
dc.date.none.fl_str_mv 2006
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_03781097_v264_n1_p125_Ayub
url http://hdl.handle.net/20.500.12110/paper_03781097_v264_n1_p125_Ayub
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv FEMS Microbiol. Lett. 2006;264(1):125-131
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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score 13.004268

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