N-linked glycoproteins are related to schizogony of the intraerythrocytic stage in Plasmodium falciparum

• Autores: Kimura, E.A.; Couto, A.S.; Peres, V.J.; Casal, O.L.; Katzin, A.M.
• Año de publicación: 1996
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: J. BIOL. CHEM. 1996;271(24):14452-14461
• Materia: asparagine linked oligosaccharide; concanavalin a; glucan synthase; glycoprotein; sepharose; tunicamycin; article; controlled study; erythrocyte; life cycle; nonhuman; plasmodium falciparum; priority journal; protein glycosylation; protein synthesis; schizont; trophozoite; Amidohydrolases; Animals; Carbon Radioisotopes; Chromatography, Affinity; Chromatography, Gel; Chromatography, Paper; Chromatography, Thin Layer; Cycloheximide; Electrophoresis, Polyacrylamide Gel; Erythrocytes; Glucose; Glycoproteins; Humans; Kinetics; Malaria, Falciparum; Mannose; Methionine; Molecular Weight; Oligosaccharides; Parasitemia; Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; Plasmodium falciparum; Protozoan Proteins; Sulfur Radioisotopes; Tunicamycin
• Institución: Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales

Although the existence of O-linked oligosaccharide residues in glycoproteins of Plasmodium falciparum has been shown, the existence of N- linked glycoproteins is still a matter of controversy and skepticism. This report demonstrates the unequivocal presence of N-linked glycoproteins in P. falciparum, principally in the ring and young trophozoite stages of the intraerythrocytic cycle. These glycoproteins lose their capacity to bind to concanavalin A-Sepharose after treatment of cultures with tunicamycin under conditions that do not affect protein synthesis. When the glycoproteins were treated with N-Glycanase®, oligosaccharides were released. It was possible to identify an N-linked glycoprotein of >200 kDa in the ring stage and also N-linked glycoproteins in the range of 200-30 kDa in the trophozoite stage. Treatment of trophozoites with 12 μM tunicamycin inhibited differentiation to the schizont stage. To our knowledge, this is the first report in the literature unequivocally showing N-linked glycoproteins in trophozoites of P. falciparum as well as their importance for the differentiation of the intraerythrocytic stages of this parasite.
Fil:Couto, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Casal, O.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.