Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line

Autores
Correa García, S.; Casas, A.; Perotti, C.; Batlle, A.; Bermúdez Moretti, M.
Año de publicación
2003
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
δ-aminolevulinic acid (ALA) is the precursor in the biosynthesis of porphyrins. The knowledge of both the regulation of ALA entrance and efflux from the cells and the control of porphyrin biosynthesis is essential to improve ALA-mediated photodynamic therapy. In this work, we studied the regulation of ALA uptake and efflux by endogenously accumulated ALA and/or porphyrins in murine mammary adenocarcinoma cells. Under our set of conditions, the haem synthesis inhibitor succinyl acetone completely prevented porphobilinogen and porphyrin synthesis from ALA, and led to an increase in the intracellular ALA pool. However, neither intracellular ALA nor porphyrin pools regulate ALA uptake or efflux during the first 15 min of the process. Based on temperature dependence data, ALA but not γ-aminobutyric acid (GABA) efflux is mediated by a diffusion mechanism. Moreover, the addition of extracellular GABA not only did not influence the rate of ALA efflux but on the contrary it affected ALA uptake, showing the contribution of a saturable mechanism for the uptake, but not for the efflux of ALA from the cells. © 2003 Cancer Research UK.
Fil:Correa García, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Casas, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Perotti, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Bermúdez Moretti, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
Br. J. Cancer 2003;89(1):173-177
Materia
δ-aminolevulinic acid
ALA efflux
ALA uptake
Photodynamic therapy
4 aminobutyric acid
aminolevulinic acid
heme derivative
porphobilinogen
porphyrin
succinylacetone
animal cell
article
breast adenocarcinoma
cancer cell culture
cell transport
controlled study
diffusion
heme synthesis
mouse
nonhuman
photodynamic therapy
priority journal
protein function
protein protein interaction
protein transport
temperature dependence
Adenocarcinoma
Aminolevulinic Acid
Animals
gamma-Aminobutyric Acid
Mammary Neoplasms, Animal
Mice
Photochemotherapy
Photosensitizing Agents
Porphyrins
Tumor Cells, Cultured
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_00070920_v89_n1_p173_CorreaGarcia
Acceder
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oai_identifier_str paperaa:paper_00070920_v89_n1_p173_CorreaGarcia
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell lineCorrea García, S.Casas, A.Perotti, C.Batlle, A.Bermúdez Moretti, M.δ-aminolevulinic acidALA effluxALA uptakePhotodynamic therapy4 aminobutyric acidaminolevulinic acidheme derivativeporphobilinogenporphyrinsuccinylacetoneanimal cellarticlebreast adenocarcinomacancer cell culturecell transportcontrolled studydiffusionheme synthesismousenonhumanphotodynamic therapypriority journalprotein functionprotein protein interactionprotein transporttemperature dependenceAdenocarcinomaAminolevulinic AcidAnimalsgamma-Aminobutyric AcidMammary Neoplasms, AnimalMicePhotochemotherapyPhotosensitizing AgentsPorphyrinsTumor Cells, Culturedδ-aminolevulinic acid (ALA) is the precursor in the biosynthesis of porphyrins. The knowledge of both the regulation of ALA entrance and efflux from the cells and the control of porphyrin biosynthesis is essential to improve ALA-mediated photodynamic therapy. In this work, we studied the regulation of ALA uptake and efflux by endogenously accumulated ALA and/or porphyrins in murine mammary adenocarcinoma cells. Under our set of conditions, the haem synthesis inhibitor succinyl acetone completely prevented porphobilinogen and porphyrin synthesis from ALA, and led to an increase in the intracellular ALA pool. However, neither intracellular ALA nor porphyrin pools regulate ALA uptake or efflux during the first 15 min of the process. Based on temperature dependence data, ALA but not γ-aminobutyric acid (GABA) efflux is mediated by a diffusion mechanism. Moreover, the addition of extracellular GABA not only did not influence the rate of ALA efflux but on the contrary it affected ALA uptake, showing the contribution of a saturable mechanism for the uptake, but not for the efflux of ALA from the cells. © 2003 Cancer Research UK.Fil:Correa García, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Casas, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Perotti, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Bermúdez Moretti, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2003info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00070920_v89_n1_p173_CorreaGarciaBr. J. Cancer 2003;89(1):173-177reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:42:57Zpaperaa:paper_00070920_v89_n1_p173_CorreaGarciaInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:42:59.041Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line
title Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line
spellingShingle Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line
Correa García, S.
δ-aminolevulinic acid
ALA efflux
ALA uptake
Photodynamic therapy
4 aminobutyric acid
aminolevulinic acid
heme derivative
porphobilinogen
porphyrin
succinylacetone
animal cell
article
breast adenocarcinoma
cancer cell culture
cell transport
controlled study
diffusion
heme synthesis
mouse
nonhuman
photodynamic therapy
priority journal
protein function
protein protein interaction
protein transport
temperature dependence
Adenocarcinoma
Aminolevulinic Acid
Animals
gamma-Aminobutyric Acid
Mammary Neoplasms, Animal
Mice
Photochemotherapy
Photosensitizing Agents
Porphyrins
Tumor Cells, Cultured
title_short Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line
title_full Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line
title_fullStr Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line
title_full_unstemmed Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line
title_sort Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line
dc.creator.none.fl_str_mv Correa García, S.
Casas, A.
Perotti, C.
Batlle, A.
Bermúdez Moretti, M.
author Correa García, S.
author_facet Correa García, S.
Casas, A.
Perotti, C.
Batlle, A.
Bermúdez Moretti, M.
author_role author
author2 Casas, A.
Perotti, C.
Batlle, A.
Bermúdez Moretti, M.
author2_role author
author
author
author
dc.subject.none.fl_str_mv δ-aminolevulinic acid
ALA efflux
ALA uptake
Photodynamic therapy
4 aminobutyric acid
aminolevulinic acid
heme derivative
porphobilinogen
porphyrin
succinylacetone
animal cell
article
breast adenocarcinoma
cancer cell culture
cell transport
controlled study
diffusion
heme synthesis
mouse
nonhuman
photodynamic therapy
priority journal
protein function
protein protein interaction
protein transport
temperature dependence
Adenocarcinoma
Aminolevulinic Acid
Animals
gamma-Aminobutyric Acid
Mammary Neoplasms, Animal
Mice
Photochemotherapy
Photosensitizing Agents
Porphyrins
Tumor Cells, Cultured
topic δ-aminolevulinic acid
ALA efflux
ALA uptake
Photodynamic therapy
4 aminobutyric acid
aminolevulinic acid
heme derivative
porphobilinogen
porphyrin
succinylacetone
animal cell
article
breast adenocarcinoma
cancer cell culture
cell transport
controlled study
diffusion
heme synthesis
mouse
nonhuman
photodynamic therapy
priority journal
protein function
protein protein interaction
protein transport
temperature dependence
Adenocarcinoma
Aminolevulinic Acid
Animals
gamma-Aminobutyric Acid
Mammary Neoplasms, Animal
Mice
Photochemotherapy
Photosensitizing Agents
Porphyrins
Tumor Cells, Cultured
dc.description.none.fl_txt_mv δ-aminolevulinic acid (ALA) is the precursor in the biosynthesis of porphyrins. The knowledge of both the regulation of ALA entrance and efflux from the cells and the control of porphyrin biosynthesis is essential to improve ALA-mediated photodynamic therapy. In this work, we studied the regulation of ALA uptake and efflux by endogenously accumulated ALA and/or porphyrins in murine mammary adenocarcinoma cells. Under our set of conditions, the haem synthesis inhibitor succinyl acetone completely prevented porphobilinogen and porphyrin synthesis from ALA, and led to an increase in the intracellular ALA pool. However, neither intracellular ALA nor porphyrin pools regulate ALA uptake or efflux during the first 15 min of the process. Based on temperature dependence data, ALA but not γ-aminobutyric acid (GABA) efflux is mediated by a diffusion mechanism. Moreover, the addition of extracellular GABA not only did not influence the rate of ALA efflux but on the contrary it affected ALA uptake, showing the contribution of a saturable mechanism for the uptake, but not for the efflux of ALA from the cells. © 2003 Cancer Research UK.
Fil:Correa García, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Casas, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Perotti, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Bermúdez Moretti, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description δ-aminolevulinic acid (ALA) is the precursor in the biosynthesis of porphyrins. The knowledge of both the regulation of ALA entrance and efflux from the cells and the control of porphyrin biosynthesis is essential to improve ALA-mediated photodynamic therapy. In this work, we studied the regulation of ALA uptake and efflux by endogenously accumulated ALA and/or porphyrins in murine mammary adenocarcinoma cells. Under our set of conditions, the haem synthesis inhibitor succinyl acetone completely prevented porphobilinogen and porphyrin synthesis from ALA, and led to an increase in the intracellular ALA pool. However, neither intracellular ALA nor porphyrin pools regulate ALA uptake or efflux during the first 15 min of the process. Based on temperature dependence data, ALA but not γ-aminobutyric acid (GABA) efflux is mediated by a diffusion mechanism. Moreover, the addition of extracellular GABA not only did not influence the rate of ALA efflux but on the contrary it affected ALA uptake, showing the contribution of a saturable mechanism for the uptake, but not for the efflux of ALA from the cells. © 2003 Cancer Research UK.
publishDate 2003
dc.date.none.fl_str_mv 2003
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_00070920_v89_n1_p173_CorreaGarcia
url http://hdl.handle.net/20.500.12110/paper_00070920_v89_n1_p173_CorreaGarcia
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Br. J. Cancer 2003;89(1):173-177
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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score 13.070432

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