RSUME, a Small RWD-Containing Protein, Enhances SUMO Conjugation and Stabilizes HIF-1α during Hypoxia

Autores
Carbia-Nagashima, A.; Gerez, J.; Perez-Castro, C.; Paez-Pereda, M.; Silberstein, S.; Stalla, G.K.; Holsboer, F.; Arzt, E.
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
SUMO conjugation to proteins is involved in the regulation of diverse cellular functions. We have identified a protein, RWD-containing sumoylation enhancer (RSUME), that enhances overall SUMO-1, -2, and -3 conjugation by interacting with the SUMO conjugase Ubc9. RSUME increases noncovalent binding of SUMO-1 to Ubc9 and enhances Ubc9 thioester formation and SUMO polymerization. RSUME enhances the sumoylation of IkB in vitro and in cultured cells, leading to an inhibition of NF-kB transcriptional activity. RSUME is induced by hypoxia and enhances the sumoylation of HIF-1α, promoting its stabilization and transcriptional activity during hypoxia. Disruption of the RWD domain structure of RSUME demonstrates that this domain is critical for RSUME action. Together, these findings point to a central role of RSUME in the regulation of sumoylation and, hence, several critical regulatory pathways in mammalian cells. © 2007 Elsevier Inc. All rights reserved.
Fil:Carbia-Nagashima, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Gerez, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Perez-Castro, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Paez-Pereda, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Silberstein, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
Cell 2007;131(2):309-323
Materia
CELLBIO
PROTEINS
cell protein
hypoxia inducible factor 1alpha
I kappa B
immunoglobulin enhancer binding protein
protein RSUME
protein Ubc9
SUMO 1 protein
sumo 2 protein
sumo 3 protein
SUMO protein
unclassified drug
animal cell
article
cell function
conjugation
controlled study
genetic transcription
human
human cell
hypoxia
mammal cell
mouse
nonhuman
nucleotide sequence
polymerization
priority journal
protein binding
protein domain
protein expression
protein protein interaction
protein stability
protein structure
rat
regulatory mechanism
sumoylation
Amino Acid Sequence
Animals
Base Sequence
Cell Hypoxia
Cell Line, Tumor
Cercopithecus aethiops
Humans
Hypoxia-Inducible Factor 1, alpha Subunit
I-kappa B Kinase
Molecular Sequence Data
NF-kappa B
Organ Specificity
Protein Binding
Small Ubiquitin-Related Modifier Proteins
SUMO-1 Protein
Transcription Factors
Ubiquitin-Conjugating Enzymes
Ubiquitins
Mammalia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_00928674_v131_n2_p309_CarbiaNagashima
Acceder
id BDUBAFCEN_8f68a112b00f23dd7992fd03de2c723d
oai_identifier_str paperaa:paper_00928674_v131_n2_p309_CarbiaNagashima
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling RSUME, a Small RWD-Containing Protein, Enhances SUMO Conjugation and Stabilizes HIF-1α during HypoxiaCarbia-Nagashima, A.Gerez, J.Perez-Castro, C.Paez-Pereda, M.Silberstein, S.Stalla, G.K.Holsboer, F.Arzt, E.CELLBIOPROTEINScell proteinhypoxia inducible factor 1alphaI kappa Bimmunoglobulin enhancer binding proteinprotein RSUMEprotein Ubc9SUMO 1 proteinsumo 2 proteinsumo 3 proteinSUMO proteinunclassified druganimal cellarticlecell functionconjugationcontrolled studygenetic transcriptionhumanhuman cellhypoxiamammal cellmousenonhumannucleotide sequencepolymerizationpriority journalprotein bindingprotein domainprotein expressionprotein protein interactionprotein stabilityprotein structureratregulatory mechanismsumoylationAmino Acid SequenceAnimalsBase SequenceCell HypoxiaCell Line, TumorCercopithecus aethiopsHumansHypoxia-Inducible Factor 1, alpha SubunitI-kappa B KinaseMolecular Sequence DataNF-kappa BOrgan SpecificityProtein BindingSmall Ubiquitin-Related Modifier ProteinsSUMO-1 ProteinTranscription FactorsUbiquitin-Conjugating EnzymesUbiquitinsMammaliaSUMO conjugation to proteins is involved in the regulation of diverse cellular functions. We have identified a protein, RWD-containing sumoylation enhancer (RSUME), that enhances overall SUMO-1, -2, and -3 conjugation by interacting with the SUMO conjugase Ubc9. RSUME increases noncovalent binding of SUMO-1 to Ubc9 and enhances Ubc9 thioester formation and SUMO polymerization. RSUME enhances the sumoylation of IkB in vitro and in cultured cells, leading to an inhibition of NF-kB transcriptional activity. RSUME is induced by hypoxia and enhances the sumoylation of HIF-1α, promoting its stabilization and transcriptional activity during hypoxia. Disruption of the RWD domain structure of RSUME demonstrates that this domain is critical for RSUME action. Together, these findings point to a central role of RSUME in the regulation of sumoylation and, hence, several critical regulatory pathways in mammalian cells. © 2007 Elsevier Inc. All rights reserved.Fil:Carbia-Nagashima, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Gerez, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Perez-Castro, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Paez-Pereda, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Silberstein, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2007info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00928674_v131_n2_p309_CarbiaNagashimaCell 2007;131(2):309-323reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-10-16T09:30:07Zpaperaa:paper_00928674_v131_n2_p309_CarbiaNagashimaInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-10-16 09:30:08.448Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv RSUME, a Small RWD-Containing Protein, Enhances SUMO Conjugation and Stabilizes HIF-1α during Hypoxia
title RSUME, a Small RWD-Containing Protein, Enhances SUMO Conjugation and Stabilizes HIF-1α during Hypoxia
spellingShingle RSUME, a Small RWD-Containing Protein, Enhances SUMO Conjugation and Stabilizes HIF-1α during Hypoxia
Carbia-Nagashima, A.
CELLBIO
PROTEINS
cell protein
hypoxia inducible factor 1alpha
I kappa B
immunoglobulin enhancer binding protein
protein RSUME
protein Ubc9
SUMO 1 protein
sumo 2 protein
sumo 3 protein
SUMO protein
unclassified drug
animal cell
article
cell function
conjugation
controlled study
genetic transcription
human
human cell
hypoxia
mammal cell
mouse
nonhuman
nucleotide sequence
polymerization
priority journal
protein binding
protein domain
protein expression
protein protein interaction
protein stability
protein structure
rat
regulatory mechanism
sumoylation
Amino Acid Sequence
Animals
Base Sequence
Cell Hypoxia
Cell Line, Tumor
Cercopithecus aethiops
Humans
Hypoxia-Inducible Factor 1, alpha Subunit
I-kappa B Kinase
Molecular Sequence Data
NF-kappa B
Organ Specificity
Protein Binding
Small Ubiquitin-Related Modifier Proteins
SUMO-1 Protein
Transcription Factors
Ubiquitin-Conjugating Enzymes
Ubiquitins
Mammalia
title_short RSUME, a Small RWD-Containing Protein, Enhances SUMO Conjugation and Stabilizes HIF-1α during Hypoxia
title_full RSUME, a Small RWD-Containing Protein, Enhances SUMO Conjugation and Stabilizes HIF-1α during Hypoxia
title_fullStr RSUME, a Small RWD-Containing Protein, Enhances SUMO Conjugation and Stabilizes HIF-1α during Hypoxia
title_full_unstemmed RSUME, a Small RWD-Containing Protein, Enhances SUMO Conjugation and Stabilizes HIF-1α during Hypoxia
title_sort RSUME, a Small RWD-Containing Protein, Enhances SUMO Conjugation and Stabilizes HIF-1α during Hypoxia
dc.creator.none.fl_str_mv Carbia-Nagashima, A.
Gerez, J.
Perez-Castro, C.
Paez-Pereda, M.
Silberstein, S.
Stalla, G.K.
Holsboer, F.
Arzt, E.
author Carbia-Nagashima, A.
author_facet Carbia-Nagashima, A.
Gerez, J.
Perez-Castro, C.
Paez-Pereda, M.
Silberstein, S.
Stalla, G.K.
Holsboer, F.
Arzt, E.
author_role author
author2 Gerez, J.
Perez-Castro, C.
Paez-Pereda, M.
Silberstein, S.
Stalla, G.K.
Holsboer, F.
Arzt, E.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CELLBIO
PROTEINS
cell protein
hypoxia inducible factor 1alpha
I kappa B
immunoglobulin enhancer binding protein
protein RSUME
protein Ubc9
SUMO 1 protein
sumo 2 protein
sumo 3 protein
SUMO protein
unclassified drug
animal cell
article
cell function
conjugation
controlled study
genetic transcription
human
human cell
hypoxia
mammal cell
mouse
nonhuman
nucleotide sequence
polymerization
priority journal
protein binding
protein domain
protein expression
protein protein interaction
protein stability
protein structure
rat
regulatory mechanism
sumoylation
Amino Acid Sequence
Animals
Base Sequence
Cell Hypoxia
Cell Line, Tumor
Cercopithecus aethiops
Humans
Hypoxia-Inducible Factor 1, alpha Subunit
I-kappa B Kinase
Molecular Sequence Data
NF-kappa B
Organ Specificity
Protein Binding
Small Ubiquitin-Related Modifier Proteins
SUMO-1 Protein
Transcription Factors
Ubiquitin-Conjugating Enzymes
Ubiquitins
Mammalia
topic CELLBIO
PROTEINS
cell protein
hypoxia inducible factor 1alpha
I kappa B
immunoglobulin enhancer binding protein
protein RSUME
protein Ubc9
SUMO 1 protein
sumo 2 protein
sumo 3 protein
SUMO protein
unclassified drug
animal cell
article
cell function
conjugation
controlled study
genetic transcription
human
human cell
hypoxia
mammal cell
mouse
nonhuman
nucleotide sequence
polymerization
priority journal
protein binding
protein domain
protein expression
protein protein interaction
protein stability
protein structure
rat
regulatory mechanism
sumoylation
Amino Acid Sequence
Animals
Base Sequence
Cell Hypoxia
Cell Line, Tumor
Cercopithecus aethiops
Humans
Hypoxia-Inducible Factor 1, alpha Subunit
I-kappa B Kinase
Molecular Sequence Data
NF-kappa B
Organ Specificity
Protein Binding
Small Ubiquitin-Related Modifier Proteins
SUMO-1 Protein
Transcription Factors
Ubiquitin-Conjugating Enzymes
Ubiquitins
Mammalia
dc.description.none.fl_txt_mv SUMO conjugation to proteins is involved in the regulation of diverse cellular functions. We have identified a protein, RWD-containing sumoylation enhancer (RSUME), that enhances overall SUMO-1, -2, and -3 conjugation by interacting with the SUMO conjugase Ubc9. RSUME increases noncovalent binding of SUMO-1 to Ubc9 and enhances Ubc9 thioester formation and SUMO polymerization. RSUME enhances the sumoylation of IkB in vitro and in cultured cells, leading to an inhibition of NF-kB transcriptional activity. RSUME is induced by hypoxia and enhances the sumoylation of HIF-1α, promoting its stabilization and transcriptional activity during hypoxia. Disruption of the RWD domain structure of RSUME demonstrates that this domain is critical for RSUME action. Together, these findings point to a central role of RSUME in the regulation of sumoylation and, hence, several critical regulatory pathways in mammalian cells. © 2007 Elsevier Inc. All rights reserved.
Fil:Carbia-Nagashima, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Gerez, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Perez-Castro, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Paez-Pereda, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Silberstein, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description SUMO conjugation to proteins is involved in the regulation of diverse cellular functions. We have identified a protein, RWD-containing sumoylation enhancer (RSUME), that enhances overall SUMO-1, -2, and -3 conjugation by interacting with the SUMO conjugase Ubc9. RSUME increases noncovalent binding of SUMO-1 to Ubc9 and enhances Ubc9 thioester formation and SUMO polymerization. RSUME enhances the sumoylation of IkB in vitro and in cultured cells, leading to an inhibition of NF-kB transcriptional activity. RSUME is induced by hypoxia and enhances the sumoylation of HIF-1α, promoting its stabilization and transcriptional activity during hypoxia. Disruption of the RWD domain structure of RSUME demonstrates that this domain is critical for RSUME action. Together, these findings point to a central role of RSUME in the regulation of sumoylation and, hence, several critical regulatory pathways in mammalian cells. © 2007 Elsevier Inc. All rights reserved.
publishDate 2007
dc.date.none.fl_str_mv 2007
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_00928674_v131_n2_p309_CarbiaNagashima
url http://hdl.handle.net/20.500.12110/paper_00928674_v131_n2_p309_CarbiaNagashima
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Cell 2007;131(2):309-323
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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score 12.712165

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