Modulation of the Akt Pathway Reveals a Novel Link with PERK/eIF2α, which Is Relevant during Hypoxia

Autores
Blaustein, M.; Pérez-Munizaga, D.; Sánchez, M.A.; Urrutia, C.; Grande, A.; Risso, G.; Srebrow, A.; Alfaro, J.; Colman-Lerner, A.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The unfolded protein response (UPR) and the Akt signaling pathway share several regulatory functions and have the capacity to determine cell outcome under specific conditions. However, both pathways have largely been studied independently. Here, we asked whether the Akt pathway regulates the UPR. To this end, we used a series of chemical compounds that modulate PI3K/Akt pathway and monitored the activity of the three UPR branches: PERK, IRE1 and ATF6. The antiproliferative and antiviral drug Akt-IV strongly and persistently activated all three branches of the UPR. We present evidence that activation of PERK/eIF2α requires Akt and that PERK is a direct Akt target. Chemical activation of this novel Akt/PERK pathway by Akt-IV leads to cell death, which was largely dependent on the presence of PERK and IRE1. Finally, we show that hypoxia-induced activation of eIF2α requires Akt, providing a physiologically relevant condition for the interaction between Akt and the PERK branch of the UPR. These data suggest the UPR and the Akt pathway signal to one another as a means of controlling cell fate. © 2013 Blaustein et al.
Fil:Blaustein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Grande, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Risso, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Srebrow, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Colman-Lerner, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
PLoS ONE 2013;8(7)
Materia
activating transcription factor 6
cell protein
initiation factor 2alpha
inositol requiring protein 1
PERK protein
phosphatidylinositol 3 kinase
protein kinase B
unclassified drug
article
cell death
cell fate
controlled study
human
human cell
hypoxia
physiological process
protein phosphorylation
protein protein interaction
protein targeting
protein unfolding
signal transduction
Cell Hypoxia
Cell Line
Cell Line, Tumor
Cell Survival
eIF-2 Kinase
Eukaryotic Initiation Factor-2
HeLa Cells
Humans
Proto-Oncogene Proteins c-akt
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_19326203_v8_n7_p_Blaustein

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oai_identifier_str paperaa:paper_19326203_v8_n7_p_Blaustein
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Modulation of the Akt Pathway Reveals a Novel Link with PERK/eIF2α, which Is Relevant during HypoxiaBlaustein, M.Pérez-Munizaga, D.Sánchez, M.A.Urrutia, C.Grande, A.Risso, G.Srebrow, A.Alfaro, J.Colman-Lerner, A.activating transcription factor 6cell proteininitiation factor 2alphainositol requiring protein 1PERK proteinphosphatidylinositol 3 kinaseprotein kinase Bunclassified drugarticlecell deathcell fatecontrolled studyhumanhuman cellhypoxiaphysiological processprotein phosphorylationprotein protein interactionprotein targetingprotein unfoldingsignal transductionCell HypoxiaCell LineCell Line, TumorCell SurvivaleIF-2 KinaseEukaryotic Initiation Factor-2HeLa CellsHumansProto-Oncogene Proteins c-aktThe unfolded protein response (UPR) and the Akt signaling pathway share several regulatory functions and have the capacity to determine cell outcome under specific conditions. However, both pathways have largely been studied independently. Here, we asked whether the Akt pathway regulates the UPR. To this end, we used a series of chemical compounds that modulate PI3K/Akt pathway and monitored the activity of the three UPR branches: PERK, IRE1 and ATF6. The antiproliferative and antiviral drug Akt-IV strongly and persistently activated all three branches of the UPR. We present evidence that activation of PERK/eIF2α requires Akt and that PERK is a direct Akt target. Chemical activation of this novel Akt/PERK pathway by Akt-IV leads to cell death, which was largely dependent on the presence of PERK and IRE1. Finally, we show that hypoxia-induced activation of eIF2α requires Akt, providing a physiologically relevant condition for the interaction between Akt and the PERK branch of the UPR. These data suggest the UPR and the Akt pathway signal to one another as a means of controlling cell fate. © 2013 Blaustein et al.Fil:Blaustein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Grande, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Risso, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Srebrow, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Colman-Lerner, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_19326203_v8_n7_p_BlausteinPLoS ONE 2013;8(7)reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:43:05Zpaperaa:paper_19326203_v8_n7_p_BlausteinInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:43:07.164Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Modulation of the Akt Pathway Reveals a Novel Link with PERK/eIF2α, which Is Relevant during Hypoxia
title Modulation of the Akt Pathway Reveals a Novel Link with PERK/eIF2α, which Is Relevant during Hypoxia
spellingShingle Modulation of the Akt Pathway Reveals a Novel Link with PERK/eIF2α, which Is Relevant during Hypoxia
Blaustein, M.
activating transcription factor 6
cell protein
initiation factor 2alpha
inositol requiring protein 1
PERK protein
phosphatidylinositol 3 kinase
protein kinase B
unclassified drug
article
cell death
cell fate
controlled study
human
human cell
hypoxia
physiological process
protein phosphorylation
protein protein interaction
protein targeting
protein unfolding
signal transduction
Cell Hypoxia
Cell Line
Cell Line, Tumor
Cell Survival
eIF-2 Kinase
Eukaryotic Initiation Factor-2
HeLa Cells
Humans
Proto-Oncogene Proteins c-akt
title_short Modulation of the Akt Pathway Reveals a Novel Link with PERK/eIF2α, which Is Relevant during Hypoxia
title_full Modulation of the Akt Pathway Reveals a Novel Link with PERK/eIF2α, which Is Relevant during Hypoxia
title_fullStr Modulation of the Akt Pathway Reveals a Novel Link with PERK/eIF2α, which Is Relevant during Hypoxia
title_full_unstemmed Modulation of the Akt Pathway Reveals a Novel Link with PERK/eIF2α, which Is Relevant during Hypoxia
title_sort Modulation of the Akt Pathway Reveals a Novel Link with PERK/eIF2α, which Is Relevant during Hypoxia
dc.creator.none.fl_str_mv Blaustein, M.
Pérez-Munizaga, D.
Sánchez, M.A.
Urrutia, C.
Grande, A.
Risso, G.
Srebrow, A.
Alfaro, J.
Colman-Lerner, A.
author Blaustein, M.
author_facet Blaustein, M.
Pérez-Munizaga, D.
Sánchez, M.A.
Urrutia, C.
Grande, A.
Risso, G.
Srebrow, A.
Alfaro, J.
Colman-Lerner, A.
author_role author
author2 Pérez-Munizaga, D.
Sánchez, M.A.
Urrutia, C.
Grande, A.
Risso, G.
Srebrow, A.
Alfaro, J.
Colman-Lerner, A.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv activating transcription factor 6
cell protein
initiation factor 2alpha
inositol requiring protein 1
PERK protein
phosphatidylinositol 3 kinase
protein kinase B
unclassified drug
article
cell death
cell fate
controlled study
human
human cell
hypoxia
physiological process
protein phosphorylation
protein protein interaction
protein targeting
protein unfolding
signal transduction
Cell Hypoxia
Cell Line
Cell Line, Tumor
Cell Survival
eIF-2 Kinase
Eukaryotic Initiation Factor-2
HeLa Cells
Humans
Proto-Oncogene Proteins c-akt
topic activating transcription factor 6
cell protein
initiation factor 2alpha
inositol requiring protein 1
PERK protein
phosphatidylinositol 3 kinase
protein kinase B
unclassified drug
article
cell death
cell fate
controlled study
human
human cell
hypoxia
physiological process
protein phosphorylation
protein protein interaction
protein targeting
protein unfolding
signal transduction
Cell Hypoxia
Cell Line
Cell Line, Tumor
Cell Survival
eIF-2 Kinase
Eukaryotic Initiation Factor-2
HeLa Cells
Humans
Proto-Oncogene Proteins c-akt
dc.description.none.fl_txt_mv The unfolded protein response (UPR) and the Akt signaling pathway share several regulatory functions and have the capacity to determine cell outcome under specific conditions. However, both pathways have largely been studied independently. Here, we asked whether the Akt pathway regulates the UPR. To this end, we used a series of chemical compounds that modulate PI3K/Akt pathway and monitored the activity of the three UPR branches: PERK, IRE1 and ATF6. The antiproliferative and antiviral drug Akt-IV strongly and persistently activated all three branches of the UPR. We present evidence that activation of PERK/eIF2α requires Akt and that PERK is a direct Akt target. Chemical activation of this novel Akt/PERK pathway by Akt-IV leads to cell death, which was largely dependent on the presence of PERK and IRE1. Finally, we show that hypoxia-induced activation of eIF2α requires Akt, providing a physiologically relevant condition for the interaction between Akt and the PERK branch of the UPR. These data suggest the UPR and the Akt pathway signal to one another as a means of controlling cell fate. © 2013 Blaustein et al.
Fil:Blaustein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Grande, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Risso, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Srebrow, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Colman-Lerner, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description The unfolded protein response (UPR) and the Akt signaling pathway share several regulatory functions and have the capacity to determine cell outcome under specific conditions. However, both pathways have largely been studied independently. Here, we asked whether the Akt pathway regulates the UPR. To this end, we used a series of chemical compounds that modulate PI3K/Akt pathway and monitored the activity of the three UPR branches: PERK, IRE1 and ATF6. The antiproliferative and antiviral drug Akt-IV strongly and persistently activated all three branches of the UPR. We present evidence that activation of PERK/eIF2α requires Akt and that PERK is a direct Akt target. Chemical activation of this novel Akt/PERK pathway by Akt-IV leads to cell death, which was largely dependent on the presence of PERK and IRE1. Finally, we show that hypoxia-induced activation of eIF2α requires Akt, providing a physiologically relevant condition for the interaction between Akt and the PERK branch of the UPR. These data suggest the UPR and the Akt pathway signal to one another as a means of controlling cell fate. © 2013 Blaustein et al.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_19326203_v8_n7_p_Blaustein
url http://hdl.handle.net/20.500.12110/paper_19326203_v8_n7_p_Blaustein
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv PLoS ONE 2013;8(7)
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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