Modulation of the Akt Pathway Reveals a Novel Link with PERK/eIF2α, which Is Relevant during Hypoxia
- Autores
- Blaustein, M.; Pérez-Munizaga, D.; Sánchez, M.A.; Urrutia, C.; Grande, A.; Risso, G.; Srebrow, A.; Alfaro, J.; Colman-Lerner, A.
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The unfolded protein response (UPR) and the Akt signaling pathway share several regulatory functions and have the capacity to determine cell outcome under specific conditions. However, both pathways have largely been studied independently. Here, we asked whether the Akt pathway regulates the UPR. To this end, we used a series of chemical compounds that modulate PI3K/Akt pathway and monitored the activity of the three UPR branches: PERK, IRE1 and ATF6. The antiproliferative and antiviral drug Akt-IV strongly and persistently activated all three branches of the UPR. We present evidence that activation of PERK/eIF2α requires Akt and that PERK is a direct Akt target. Chemical activation of this novel Akt/PERK pathway by Akt-IV leads to cell death, which was largely dependent on the presence of PERK and IRE1. Finally, we show that hypoxia-induced activation of eIF2α requires Akt, providing a physiologically relevant condition for the interaction between Akt and the PERK branch of the UPR. These data suggest the UPR and the Akt pathway signal to one another as a means of controlling cell fate. © 2013 Blaustein et al.
Fil:Blaustein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Grande, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Risso, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Srebrow, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Colman-Lerner, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. - Fuente
- PLoS ONE 2013;8(7)
- Materia
-
activating transcription factor 6
cell protein
initiation factor 2alpha
inositol requiring protein 1
PERK protein
phosphatidylinositol 3 kinase
protein kinase B
unclassified drug
article
cell death
cell fate
controlled study
human
human cell
hypoxia
physiological process
protein phosphorylation
protein protein interaction
protein targeting
protein unfolding
signal transduction
Cell Hypoxia
Cell Line
Cell Line, Tumor
Cell Survival
eIF-2 Kinase
Eukaryotic Initiation Factor-2
HeLa Cells
Humans
Proto-Oncogene Proteins c-akt - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/2.5/ar
- Repositorio
- Institución
- Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
- OAI Identificador
- paperaa:paper_19326203_v8_n7_p_Blaustein
Ver los metadatos del registro completo
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Modulation of the Akt Pathway Reveals a Novel Link with PERK/eIF2α, which Is Relevant during HypoxiaBlaustein, M.Pérez-Munizaga, D.Sánchez, M.A.Urrutia, C.Grande, A.Risso, G.Srebrow, A.Alfaro, J.Colman-Lerner, A.activating transcription factor 6cell proteininitiation factor 2alphainositol requiring protein 1PERK proteinphosphatidylinositol 3 kinaseprotein kinase Bunclassified drugarticlecell deathcell fatecontrolled studyhumanhuman cellhypoxiaphysiological processprotein phosphorylationprotein protein interactionprotein targetingprotein unfoldingsignal transductionCell HypoxiaCell LineCell Line, TumorCell SurvivaleIF-2 KinaseEukaryotic Initiation Factor-2HeLa CellsHumansProto-Oncogene Proteins c-aktThe unfolded protein response (UPR) and the Akt signaling pathway share several regulatory functions and have the capacity to determine cell outcome under specific conditions. However, both pathways have largely been studied independently. Here, we asked whether the Akt pathway regulates the UPR. To this end, we used a series of chemical compounds that modulate PI3K/Akt pathway and monitored the activity of the three UPR branches: PERK, IRE1 and ATF6. The antiproliferative and antiviral drug Akt-IV strongly and persistently activated all three branches of the UPR. We present evidence that activation of PERK/eIF2α requires Akt and that PERK is a direct Akt target. Chemical activation of this novel Akt/PERK pathway by Akt-IV leads to cell death, which was largely dependent on the presence of PERK and IRE1. Finally, we show that hypoxia-induced activation of eIF2α requires Akt, providing a physiologically relevant condition for the interaction between Akt and the PERK branch of the UPR. These data suggest the UPR and the Akt pathway signal to one another as a means of controlling cell fate. © 2013 Blaustein et al.Fil:Blaustein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Grande, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Risso, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Srebrow, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Colman-Lerner, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_19326203_v8_n7_p_BlausteinPLoS ONE 2013;8(7)reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:43:05Zpaperaa:paper_19326203_v8_n7_p_BlausteinInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:43:07.164Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse |
dc.title.none.fl_str_mv |
Modulation of the Akt Pathway Reveals a Novel Link with PERK/eIF2α, which Is Relevant during Hypoxia |
title |
Modulation of the Akt Pathway Reveals a Novel Link with PERK/eIF2α, which Is Relevant during Hypoxia |
spellingShingle |
Modulation of the Akt Pathway Reveals a Novel Link with PERK/eIF2α, which Is Relevant during Hypoxia Blaustein, M. activating transcription factor 6 cell protein initiation factor 2alpha inositol requiring protein 1 PERK protein phosphatidylinositol 3 kinase protein kinase B unclassified drug article cell death cell fate controlled study human human cell hypoxia physiological process protein phosphorylation protein protein interaction protein targeting protein unfolding signal transduction Cell Hypoxia Cell Line Cell Line, Tumor Cell Survival eIF-2 Kinase Eukaryotic Initiation Factor-2 HeLa Cells Humans Proto-Oncogene Proteins c-akt |
title_short |
Modulation of the Akt Pathway Reveals a Novel Link with PERK/eIF2α, which Is Relevant during Hypoxia |
title_full |
Modulation of the Akt Pathway Reveals a Novel Link with PERK/eIF2α, which Is Relevant during Hypoxia |
title_fullStr |
Modulation of the Akt Pathway Reveals a Novel Link with PERK/eIF2α, which Is Relevant during Hypoxia |
title_full_unstemmed |
Modulation of the Akt Pathway Reveals a Novel Link with PERK/eIF2α, which Is Relevant during Hypoxia |
title_sort |
Modulation of the Akt Pathway Reveals a Novel Link with PERK/eIF2α, which Is Relevant during Hypoxia |
dc.creator.none.fl_str_mv |
Blaustein, M. Pérez-Munizaga, D. Sánchez, M.A. Urrutia, C. Grande, A. Risso, G. Srebrow, A. Alfaro, J. Colman-Lerner, A. |
author |
Blaustein, M. |
author_facet |
Blaustein, M. Pérez-Munizaga, D. Sánchez, M.A. Urrutia, C. Grande, A. Risso, G. Srebrow, A. Alfaro, J. Colman-Lerner, A. |
author_role |
author |
author2 |
Pérez-Munizaga, D. Sánchez, M.A. Urrutia, C. Grande, A. Risso, G. Srebrow, A. Alfaro, J. Colman-Lerner, A. |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
activating transcription factor 6 cell protein initiation factor 2alpha inositol requiring protein 1 PERK protein phosphatidylinositol 3 kinase protein kinase B unclassified drug article cell death cell fate controlled study human human cell hypoxia physiological process protein phosphorylation protein protein interaction protein targeting protein unfolding signal transduction Cell Hypoxia Cell Line Cell Line, Tumor Cell Survival eIF-2 Kinase Eukaryotic Initiation Factor-2 HeLa Cells Humans Proto-Oncogene Proteins c-akt |
topic |
activating transcription factor 6 cell protein initiation factor 2alpha inositol requiring protein 1 PERK protein phosphatidylinositol 3 kinase protein kinase B unclassified drug article cell death cell fate controlled study human human cell hypoxia physiological process protein phosphorylation protein protein interaction protein targeting protein unfolding signal transduction Cell Hypoxia Cell Line Cell Line, Tumor Cell Survival eIF-2 Kinase Eukaryotic Initiation Factor-2 HeLa Cells Humans Proto-Oncogene Proteins c-akt |
dc.description.none.fl_txt_mv |
The unfolded protein response (UPR) and the Akt signaling pathway share several regulatory functions and have the capacity to determine cell outcome under specific conditions. However, both pathways have largely been studied independently. Here, we asked whether the Akt pathway regulates the UPR. To this end, we used a series of chemical compounds that modulate PI3K/Akt pathway and monitored the activity of the three UPR branches: PERK, IRE1 and ATF6. The antiproliferative and antiviral drug Akt-IV strongly and persistently activated all three branches of the UPR. We present evidence that activation of PERK/eIF2α requires Akt and that PERK is a direct Akt target. Chemical activation of this novel Akt/PERK pathway by Akt-IV leads to cell death, which was largely dependent on the presence of PERK and IRE1. Finally, we show that hypoxia-induced activation of eIF2α requires Akt, providing a physiologically relevant condition for the interaction between Akt and the PERK branch of the UPR. These data suggest the UPR and the Akt pathway signal to one another as a means of controlling cell fate. © 2013 Blaustein et al. Fil:Blaustein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Grande, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Risso, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Srebrow, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Colman-Lerner, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. |
description |
The unfolded protein response (UPR) and the Akt signaling pathway share several regulatory functions and have the capacity to determine cell outcome under specific conditions. However, both pathways have largely been studied independently. Here, we asked whether the Akt pathway regulates the UPR. To this end, we used a series of chemical compounds that modulate PI3K/Akt pathway and monitored the activity of the three UPR branches: PERK, IRE1 and ATF6. The antiproliferative and antiviral drug Akt-IV strongly and persistently activated all three branches of the UPR. We present evidence that activation of PERK/eIF2α requires Akt and that PERK is a direct Akt target. Chemical activation of this novel Akt/PERK pathway by Akt-IV leads to cell death, which was largely dependent on the presence of PERK and IRE1. Finally, we show that hypoxia-induced activation of eIF2α requires Akt, providing a physiologically relevant condition for the interaction between Akt and the PERK branch of the UPR. These data suggest the UPR and the Akt pathway signal to one another as a means of controlling cell fate. © 2013 Blaustein et al. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12110/paper_19326203_v8_n7_p_Blaustein |
url |
http://hdl.handle.net/20.500.12110/paper_19326203_v8_n7_p_Blaustein |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/2.5/ar |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
PLoS ONE 2013;8(7) reponame:Biblioteca Digital (UBA-FCEN) instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales instacron:UBA-FCEN |
reponame_str |
Biblioteca Digital (UBA-FCEN) |
collection |
Biblioteca Digital (UBA-FCEN) |
instname_str |
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
instacron_str |
UBA-FCEN |
institution |
UBA-FCEN |
repository.name.fl_str_mv |
Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
repository.mail.fl_str_mv |
ana@bl.fcen.uba.ar |
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13.070432 |