Optimization of biomass production of a mutant of Yarrowia lipolytica with an increased lipase activity using raw glycerol

• Autores: Galvagno, M.A.; Iannone, L.J.; Bianchi, J.; Kronberg, F.; Rost, E.; Carstens, M.R.; Cerrutti, P.
• Año de publicación: 2011
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Rev. Argent. Microbiol. 2011;43(3):218-225
• Materia: Biomass; Fatty acids; Glycerol; Lipase; Yarrowia lipolytica mutant; biodiesel; carbon; fatty acid; glycerol; olive oil; organic nitrogen; triacylglycerol lipase; article; batch fermentation; batch reactor; biomass production; controlled study; enzyme activity; enzyme synthesis; fermentation optimization; fungus mutant; nonhuman; process optimization; response surface method; Yarrowia lipolytica; Biofuels; Biomass; Bioreactors; Culture Media; Culture Media, Conditioned; DNA, Fungal; DNA, Intergenic; Fermentation; Fungal Proteins; Genes, Fungal; Glycerol; Hyphae; Industrial Microbiology; Lipase; Mycology; Yarrowia
• Institución: Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales

The yeast Yarrowia lipolytica accumulates oils and is able to produce extracellular lipases when growing in different carbon sources including glycerol, the principal by-product of the biodiesel industry. In this study, biomass production of a novel mutant strain of Y. lipolytica was statistically optimized by Response Surface Methodology in media containing biodiesel-derived glycerol as main carbon source. This strain exhibited distinctive morphological and fatty acid profile characteristics, and showed an increased extracellular lipase activity. An organic source of nitrogen and the addition of 1.0 g/l olive oil were necessary for significant lipase production. Plackett-Burman and Central Composite Statistical Designs were employed for screening and optimization of fermentation in shaken flasks cultures, and the maximum values obtained were 16.1 g/l for biomass and 12.2 Units/ml for lipase, respectively. Optimized batch bioprocess was thereafter scaled in aerated bioreactors and the values reached for lipase specific activity after 95 % of the glycerol had been consumed, were three-fold higher than those obtained in shaken flasks cultures. A sustainable bioprocess to obtain biomass and extracellular lipase activity was attained by maximizing the use of the by-products of biodiesel industry.
Fil:Galvagno, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Iannone, L.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Cerrutti, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.