Inhibitory effect of leptin on the rat ovary during the ovulatory process
- Autores
- Ricci, A.G.; Di Yorio, M.P.; Faletti, A.G.
- Año de publicación
- 2006
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The aims of this study were to investigate the negative action of leptin on some intraovarian ovulatory mediators during the ovulatory process and to assess whether leptin is able to alter the expression of its ovarian receptors. Immature rats primed with gonadotrophins were used to induce ovulation. Serum leptin concentration was diminished 4 h after human chorionic gonadotrophin (hCG) administration, whereas the ovarian expression of leptin receptors, measured by western blot, was increased by the gonadotrophin treatment. Serum progesterone level, ovulation rate and ovarian prostaglandin E (PGE) content were reduced in rats primed with equine chorionic gonadotrophin (eCG)/hCG and treated with acute doses of leptin (five doses of 5 μg each). These inhibitory effects were confirmed by in vitro studies, where the presence of leptin reduced the concentrations of progesterone, PGE and nitrites in the media of both ovarian explants and preovulatory follicle cultures. We also investigated whether these negative effects were mediated by changes in the expression of the ovarian leptin receptors. Since leptin treatment did not alter the expression of ovarian leptin receptor, the inhibitory effect of leptin on the ovulatory process may not be mediated by changes in the expression of its receptors at ovarian level, at least at the concentrations assayed. In summary, the ovulatory process was significantly inhibited in response to an acute treatment with leptin, and this effect may be due, at least in part, to the direct or indirect impairment of some ovarian factors, such as prostaglandins and nitric oxide. © 2006 Society for Reproduction and Fertility.
Fil:Ricci, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Di Yorio, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Faletti, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. - Fuente
- Reproduction 2006;132(5):771-780
- Materia
-
chorionic gonadotropin
leptin
leptin receptor
nitric oxide
nitrite
progesterone
prostaglandin E
animal experiment
animal tissue
article
controlled study
culture medium
explant
female
hormone action
hormone blood level
in vitro study
nonhuman
ovary follicle
ovulation
ovulation induction
priority journal
progesterone blood level
protein expression
rat
tissue culture
Western blotting
Animals
Blotting, Western
Depression, Chemical
Female
Gonadotropins, Equine
Leptin
Ovary
Ovulation
Rats
Rats, Sprague-Dawley
Receptors, Cell Surface
Sexual Maturation
Tissue Culture Techniques
Equidae - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/2.5/ar
- Repositorio
- Institución
- Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
- OAI Identificador
- paperaa:paper_14701626_v132_n5_p771_Ricci
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Inhibitory effect of leptin on the rat ovary during the ovulatory processRicci, A.G.Di Yorio, M.P.Faletti, A.G.chorionic gonadotropinleptinleptin receptornitric oxidenitriteprogesteroneprostaglandin Eanimal experimentanimal tissuearticlecontrolled studyculture mediumexplantfemalehormone actionhormone blood levelin vitro studynonhumanovary follicleovulationovulation inductionpriority journalprogesterone blood levelprotein expressionrattissue cultureWestern blottingAnimalsBlotting, WesternDepression, ChemicalFemaleGonadotropins, EquineLeptinOvaryOvulationRatsRats, Sprague-DawleyReceptors, Cell SurfaceSexual MaturationTissue Culture TechniquesEquidaeThe aims of this study were to investigate the negative action of leptin on some intraovarian ovulatory mediators during the ovulatory process and to assess whether leptin is able to alter the expression of its ovarian receptors. Immature rats primed with gonadotrophins were used to induce ovulation. Serum leptin concentration was diminished 4 h after human chorionic gonadotrophin (hCG) administration, whereas the ovarian expression of leptin receptors, measured by western blot, was increased by the gonadotrophin treatment. Serum progesterone level, ovulation rate and ovarian prostaglandin E (PGE) content were reduced in rats primed with equine chorionic gonadotrophin (eCG)/hCG and treated with acute doses of leptin (five doses of 5 μg each). These inhibitory effects were confirmed by in vitro studies, where the presence of leptin reduced the concentrations of progesterone, PGE and nitrites in the media of both ovarian explants and preovulatory follicle cultures. We also investigated whether these negative effects were mediated by changes in the expression of the ovarian leptin receptors. Since leptin treatment did not alter the expression of ovarian leptin receptor, the inhibitory effect of leptin on the ovulatory process may not be mediated by changes in the expression of its receptors at ovarian level, at least at the concentrations assayed. In summary, the ovulatory process was significantly inhibited in response to an acute treatment with leptin, and this effect may be due, at least in part, to the direct or indirect impairment of some ovarian factors, such as prostaglandins and nitric oxide. © 2006 Society for Reproduction and Fertility.Fil:Ricci, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Di Yorio, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Faletti, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2006info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_14701626_v132_n5_p771_RicciReproduction 2006;132(5):771-780reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-04T09:48:40Zpaperaa:paper_14701626_v132_n5_p771_RicciInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-04 09:48:42.883Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse |
dc.title.none.fl_str_mv |
Inhibitory effect of leptin on the rat ovary during the ovulatory process |
title |
Inhibitory effect of leptin on the rat ovary during the ovulatory process |
spellingShingle |
Inhibitory effect of leptin on the rat ovary during the ovulatory process Ricci, A.G. chorionic gonadotropin leptin leptin receptor nitric oxide nitrite progesterone prostaglandin E animal experiment animal tissue article controlled study culture medium explant female hormone action hormone blood level in vitro study nonhuman ovary follicle ovulation ovulation induction priority journal progesterone blood level protein expression rat tissue culture Western blotting Animals Blotting, Western Depression, Chemical Female Gonadotropins, Equine Leptin Ovary Ovulation Rats Rats, Sprague-Dawley Receptors, Cell Surface Sexual Maturation Tissue Culture Techniques Equidae |
title_short |
Inhibitory effect of leptin on the rat ovary during the ovulatory process |
title_full |
Inhibitory effect of leptin on the rat ovary during the ovulatory process |
title_fullStr |
Inhibitory effect of leptin on the rat ovary during the ovulatory process |
title_full_unstemmed |
Inhibitory effect of leptin on the rat ovary during the ovulatory process |
title_sort |
Inhibitory effect of leptin on the rat ovary during the ovulatory process |
dc.creator.none.fl_str_mv |
Ricci, A.G. Di Yorio, M.P. Faletti, A.G. |
author |
Ricci, A.G. |
author_facet |
Ricci, A.G. Di Yorio, M.P. Faletti, A.G. |
author_role |
author |
author2 |
Di Yorio, M.P. Faletti, A.G. |
author2_role |
author author |
dc.subject.none.fl_str_mv |
chorionic gonadotropin leptin leptin receptor nitric oxide nitrite progesterone prostaglandin E animal experiment animal tissue article controlled study culture medium explant female hormone action hormone blood level in vitro study nonhuman ovary follicle ovulation ovulation induction priority journal progesterone blood level protein expression rat tissue culture Western blotting Animals Blotting, Western Depression, Chemical Female Gonadotropins, Equine Leptin Ovary Ovulation Rats Rats, Sprague-Dawley Receptors, Cell Surface Sexual Maturation Tissue Culture Techniques Equidae |
topic |
chorionic gonadotropin leptin leptin receptor nitric oxide nitrite progesterone prostaglandin E animal experiment animal tissue article controlled study culture medium explant female hormone action hormone blood level in vitro study nonhuman ovary follicle ovulation ovulation induction priority journal progesterone blood level protein expression rat tissue culture Western blotting Animals Blotting, Western Depression, Chemical Female Gonadotropins, Equine Leptin Ovary Ovulation Rats Rats, Sprague-Dawley Receptors, Cell Surface Sexual Maturation Tissue Culture Techniques Equidae |
dc.description.none.fl_txt_mv |
The aims of this study were to investigate the negative action of leptin on some intraovarian ovulatory mediators during the ovulatory process and to assess whether leptin is able to alter the expression of its ovarian receptors. Immature rats primed with gonadotrophins were used to induce ovulation. Serum leptin concentration was diminished 4 h after human chorionic gonadotrophin (hCG) administration, whereas the ovarian expression of leptin receptors, measured by western blot, was increased by the gonadotrophin treatment. Serum progesterone level, ovulation rate and ovarian prostaglandin E (PGE) content were reduced in rats primed with equine chorionic gonadotrophin (eCG)/hCG and treated with acute doses of leptin (five doses of 5 μg each). These inhibitory effects were confirmed by in vitro studies, where the presence of leptin reduced the concentrations of progesterone, PGE and nitrites in the media of both ovarian explants and preovulatory follicle cultures. We also investigated whether these negative effects were mediated by changes in the expression of the ovarian leptin receptors. Since leptin treatment did not alter the expression of ovarian leptin receptor, the inhibitory effect of leptin on the ovulatory process may not be mediated by changes in the expression of its receptors at ovarian level, at least at the concentrations assayed. In summary, the ovulatory process was significantly inhibited in response to an acute treatment with leptin, and this effect may be due, at least in part, to the direct or indirect impairment of some ovarian factors, such as prostaglandins and nitric oxide. © 2006 Society for Reproduction and Fertility. Fil:Ricci, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Di Yorio, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Faletti, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. |
description |
The aims of this study were to investigate the negative action of leptin on some intraovarian ovulatory mediators during the ovulatory process and to assess whether leptin is able to alter the expression of its ovarian receptors. Immature rats primed with gonadotrophins were used to induce ovulation. Serum leptin concentration was diminished 4 h after human chorionic gonadotrophin (hCG) administration, whereas the ovarian expression of leptin receptors, measured by western blot, was increased by the gonadotrophin treatment. Serum progesterone level, ovulation rate and ovarian prostaglandin E (PGE) content were reduced in rats primed with equine chorionic gonadotrophin (eCG)/hCG and treated with acute doses of leptin (five doses of 5 μg each). These inhibitory effects were confirmed by in vitro studies, where the presence of leptin reduced the concentrations of progesterone, PGE and nitrites in the media of both ovarian explants and preovulatory follicle cultures. We also investigated whether these negative effects were mediated by changes in the expression of the ovarian leptin receptors. Since leptin treatment did not alter the expression of ovarian leptin receptor, the inhibitory effect of leptin on the ovulatory process may not be mediated by changes in the expression of its receptors at ovarian level, at least at the concentrations assayed. In summary, the ovulatory process was significantly inhibited in response to an acute treatment with leptin, and this effect may be due, at least in part, to the direct or indirect impairment of some ovarian factors, such as prostaglandins and nitric oxide. © 2006 Society for Reproduction and Fertility. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12110/paper_14701626_v132_n5_p771_Ricci |
url |
http://hdl.handle.net/20.500.12110/paper_14701626_v132_n5_p771_Ricci |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/2.5/ar |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Reproduction 2006;132(5):771-780 reponame:Biblioteca Digital (UBA-FCEN) instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales instacron:UBA-FCEN |
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Biblioteca Digital (UBA-FCEN) |
instname_str |
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
instacron_str |
UBA-FCEN |
institution |
UBA-FCEN |
repository.name.fl_str_mv |
Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
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