Inhibitory effect of leptin on the rat ovary during the ovulatory process

Autores
Ricci, A.G.; Di Yorio, M.P.; Faletti, A.G.
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aims of this study were to investigate the negative action of leptin on some intraovarian ovulatory mediators during the ovulatory process and to assess whether leptin is able to alter the expression of its ovarian receptors. Immature rats primed with gonadotrophins were used to induce ovulation. Serum leptin concentration was diminished 4 h after human chorionic gonadotrophin (hCG) administration, whereas the ovarian expression of leptin receptors, measured by western blot, was increased by the gonadotrophin treatment. Serum progesterone level, ovulation rate and ovarian prostaglandin E (PGE) content were reduced in rats primed with equine chorionic gonadotrophin (eCG)/hCG and treated with acute doses of leptin (five doses of 5 μg each). These inhibitory effects were confirmed by in vitro studies, where the presence of leptin reduced the concentrations of progesterone, PGE and nitrites in the media of both ovarian explants and preovulatory follicle cultures. We also investigated whether these negative effects were mediated by changes in the expression of the ovarian leptin receptors. Since leptin treatment did not alter the expression of ovarian leptin receptor, the inhibitory effect of leptin on the ovulatory process may not be mediated by changes in the expression of its receptors at ovarian level, at least at the concentrations assayed. In summary, the ovulatory process was significantly inhibited in response to an acute treatment with leptin, and this effect may be due, at least in part, to the direct or indirect impairment of some ovarian factors, such as prostaglandins and nitric oxide. © 2006 Society for Reproduction and Fertility.
Fil:Ricci, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Di Yorio, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Faletti, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
Reproduction 2006;132(5):771-780
Materia
chorionic gonadotropin
leptin
leptin receptor
nitric oxide
nitrite
progesterone
prostaglandin E
animal experiment
animal tissue
article
controlled study
culture medium
explant
female
hormone action
hormone blood level
in vitro study
nonhuman
ovary follicle
ovulation
ovulation induction
priority journal
progesterone blood level
protein expression
rat
tissue culture
Western blotting
Animals
Blotting, Western
Depression, Chemical
Female
Gonadotropins, Equine
Leptin
Ovary
Ovulation
Rats
Rats, Sprague-Dawley
Receptors, Cell Surface
Sexual Maturation
Tissue Culture Techniques
Equidae
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_14701626_v132_n5_p771_Ricci

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oai_identifier_str paperaa:paper_14701626_v132_n5_p771_Ricci
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Inhibitory effect of leptin on the rat ovary during the ovulatory processRicci, A.G.Di Yorio, M.P.Faletti, A.G.chorionic gonadotropinleptinleptin receptornitric oxidenitriteprogesteroneprostaglandin Eanimal experimentanimal tissuearticlecontrolled studyculture mediumexplantfemalehormone actionhormone blood levelin vitro studynonhumanovary follicleovulationovulation inductionpriority journalprogesterone blood levelprotein expressionrattissue cultureWestern blottingAnimalsBlotting, WesternDepression, ChemicalFemaleGonadotropins, EquineLeptinOvaryOvulationRatsRats, Sprague-DawleyReceptors, Cell SurfaceSexual MaturationTissue Culture TechniquesEquidaeThe aims of this study were to investigate the negative action of leptin on some intraovarian ovulatory mediators during the ovulatory process and to assess whether leptin is able to alter the expression of its ovarian receptors. Immature rats primed with gonadotrophins were used to induce ovulation. Serum leptin concentration was diminished 4 h after human chorionic gonadotrophin (hCG) administration, whereas the ovarian expression of leptin receptors, measured by western blot, was increased by the gonadotrophin treatment. Serum progesterone level, ovulation rate and ovarian prostaglandin E (PGE) content were reduced in rats primed with equine chorionic gonadotrophin (eCG)/hCG and treated with acute doses of leptin (five doses of 5 μg each). These inhibitory effects were confirmed by in vitro studies, where the presence of leptin reduced the concentrations of progesterone, PGE and nitrites in the media of both ovarian explants and preovulatory follicle cultures. We also investigated whether these negative effects were mediated by changes in the expression of the ovarian leptin receptors. Since leptin treatment did not alter the expression of ovarian leptin receptor, the inhibitory effect of leptin on the ovulatory process may not be mediated by changes in the expression of its receptors at ovarian level, at least at the concentrations assayed. In summary, the ovulatory process was significantly inhibited in response to an acute treatment with leptin, and this effect may be due, at least in part, to the direct or indirect impairment of some ovarian factors, such as prostaglandins and nitric oxide. © 2006 Society for Reproduction and Fertility.Fil:Ricci, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Di Yorio, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Faletti, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2006info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_14701626_v132_n5_p771_RicciReproduction 2006;132(5):771-780reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-04T09:48:40Zpaperaa:paper_14701626_v132_n5_p771_RicciInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-04 09:48:42.883Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Inhibitory effect of leptin on the rat ovary during the ovulatory process
title Inhibitory effect of leptin on the rat ovary during the ovulatory process
spellingShingle Inhibitory effect of leptin on the rat ovary during the ovulatory process
Ricci, A.G.
chorionic gonadotropin
leptin
leptin receptor
nitric oxide
nitrite
progesterone
prostaglandin E
animal experiment
animal tissue
article
controlled study
culture medium
explant
female
hormone action
hormone blood level
in vitro study
nonhuman
ovary follicle
ovulation
ovulation induction
priority journal
progesterone blood level
protein expression
rat
tissue culture
Western blotting
Animals
Blotting, Western
Depression, Chemical
Female
Gonadotropins, Equine
Leptin
Ovary
Ovulation
Rats
Rats, Sprague-Dawley
Receptors, Cell Surface
Sexual Maturation
Tissue Culture Techniques
Equidae
title_short Inhibitory effect of leptin on the rat ovary during the ovulatory process
title_full Inhibitory effect of leptin on the rat ovary during the ovulatory process
title_fullStr Inhibitory effect of leptin on the rat ovary during the ovulatory process
title_full_unstemmed Inhibitory effect of leptin on the rat ovary during the ovulatory process
title_sort Inhibitory effect of leptin on the rat ovary during the ovulatory process
dc.creator.none.fl_str_mv Ricci, A.G.
Di Yorio, M.P.
Faletti, A.G.
author Ricci, A.G.
author_facet Ricci, A.G.
Di Yorio, M.P.
Faletti, A.G.
author_role author
author2 Di Yorio, M.P.
Faletti, A.G.
author2_role author
author
dc.subject.none.fl_str_mv chorionic gonadotropin
leptin
leptin receptor
nitric oxide
nitrite
progesterone
prostaglandin E
animal experiment
animal tissue
article
controlled study
culture medium
explant
female
hormone action
hormone blood level
in vitro study
nonhuman
ovary follicle
ovulation
ovulation induction
priority journal
progesterone blood level
protein expression
rat
tissue culture
Western blotting
Animals
Blotting, Western
Depression, Chemical
Female
Gonadotropins, Equine
Leptin
Ovary
Ovulation
Rats
Rats, Sprague-Dawley
Receptors, Cell Surface
Sexual Maturation
Tissue Culture Techniques
Equidae
topic chorionic gonadotropin
leptin
leptin receptor
nitric oxide
nitrite
progesterone
prostaglandin E
animal experiment
animal tissue
article
controlled study
culture medium
explant
female
hormone action
hormone blood level
in vitro study
nonhuman
ovary follicle
ovulation
ovulation induction
priority journal
progesterone blood level
protein expression
rat
tissue culture
Western blotting
Animals
Blotting, Western
Depression, Chemical
Female
Gonadotropins, Equine
Leptin
Ovary
Ovulation
Rats
Rats, Sprague-Dawley
Receptors, Cell Surface
Sexual Maturation
Tissue Culture Techniques
Equidae
dc.description.none.fl_txt_mv The aims of this study were to investigate the negative action of leptin on some intraovarian ovulatory mediators during the ovulatory process and to assess whether leptin is able to alter the expression of its ovarian receptors. Immature rats primed with gonadotrophins were used to induce ovulation. Serum leptin concentration was diminished 4 h after human chorionic gonadotrophin (hCG) administration, whereas the ovarian expression of leptin receptors, measured by western blot, was increased by the gonadotrophin treatment. Serum progesterone level, ovulation rate and ovarian prostaglandin E (PGE) content were reduced in rats primed with equine chorionic gonadotrophin (eCG)/hCG and treated with acute doses of leptin (five doses of 5 μg each). These inhibitory effects were confirmed by in vitro studies, where the presence of leptin reduced the concentrations of progesterone, PGE and nitrites in the media of both ovarian explants and preovulatory follicle cultures. We also investigated whether these negative effects were mediated by changes in the expression of the ovarian leptin receptors. Since leptin treatment did not alter the expression of ovarian leptin receptor, the inhibitory effect of leptin on the ovulatory process may not be mediated by changes in the expression of its receptors at ovarian level, at least at the concentrations assayed. In summary, the ovulatory process was significantly inhibited in response to an acute treatment with leptin, and this effect may be due, at least in part, to the direct or indirect impairment of some ovarian factors, such as prostaglandins and nitric oxide. © 2006 Society for Reproduction and Fertility.
Fil:Ricci, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Di Yorio, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Faletti, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description The aims of this study were to investigate the negative action of leptin on some intraovarian ovulatory mediators during the ovulatory process and to assess whether leptin is able to alter the expression of its ovarian receptors. Immature rats primed with gonadotrophins were used to induce ovulation. Serum leptin concentration was diminished 4 h after human chorionic gonadotrophin (hCG) administration, whereas the ovarian expression of leptin receptors, measured by western blot, was increased by the gonadotrophin treatment. Serum progesterone level, ovulation rate and ovarian prostaglandin E (PGE) content were reduced in rats primed with equine chorionic gonadotrophin (eCG)/hCG and treated with acute doses of leptin (five doses of 5 μg each). These inhibitory effects were confirmed by in vitro studies, where the presence of leptin reduced the concentrations of progesterone, PGE and nitrites in the media of both ovarian explants and preovulatory follicle cultures. We also investigated whether these negative effects were mediated by changes in the expression of the ovarian leptin receptors. Since leptin treatment did not alter the expression of ovarian leptin receptor, the inhibitory effect of leptin on the ovulatory process may not be mediated by changes in the expression of its receptors at ovarian level, at least at the concentrations assayed. In summary, the ovulatory process was significantly inhibited in response to an acute treatment with leptin, and this effect may be due, at least in part, to the direct or indirect impairment of some ovarian factors, such as prostaglandins and nitric oxide. © 2006 Society for Reproduction and Fertility.
publishDate 2006
dc.date.none.fl_str_mv 2006
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_14701626_v132_n5_p771_Ricci
url http://hdl.handle.net/20.500.12110/paper_14701626_v132_n5_p771_Ricci
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Reproduction 2006;132(5):771-780
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
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