Developmental Programming: Does Prenatal Steroid Excess Disrupt the Ovarian VEGF System in Sheep?1

Autores
Ortega, Hugo Hector; Veiga-Lopez, A.; Sreedharan, S.; Velázquez, Melisa María del Luján; Salvetti, Natalia Raquel; Padmanabhan, Vasantha
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Prenatal testosterone (T), but not dihydrotestosterone (DHT), excess disrupts ovarian cyclicity and increases follicular recruitment and persistence. We hypothesized that the disruption in the vascular endothelial growth factor (VEGF) system contributes to the enhancement of follicular recruitment and persistence in prenatal T-treated sheep. Impact of T / DHT treatments from days 30-90 of gestation on VEGFA, VEGFB, and their receptor (VEGFR-1 (FLT1), VEGFR-2 (KDR), and VEGFR-3 (FLT4)) protein expression was examined by immunohistochemistry on fetal day 90, 140, 22 weeks, 10 months (postpubertal), and 21 months (adult) of age. Arterial morphometry was performed in fetal day 140 and postpubertal ovaries. VEGFA and VEGFB expression were found in granulosa cells at all stages of follicular development with increased expression in antral follicles. VEGFA was present in theca interna, while VEGFB was present in theca interna / externa, and stromal cells. All 3 receptors were expressed in the granulosa, theca, and stromal cells during all stages of follicular development. VEGFR-3 increased with follicular differentiation with the highest level seen in the granulosa cells of antral follicles. None of the members of the VEGF family or their receptor expression were altered by age, or prenatal T / DHT treatments. At fetal day 140, area, wall thickness, and wall area of arteries from the ovarian hilum were larger in prenatal T- and DHT-treated females, suggestive of early androgenic programming of arterial differentiation. This may facilitate increased delivery of endocrine factors and thus indirectly contribute to the development of multifollicular phenotype.
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Veiga-Lopez, A.. University of Michigan; Estados Unidos
Fil: Sreedharan, S.. University of Michigan; Estados Unidos
Fil: Velázquez, Melisa María del Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Salvetti, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Padmanabhan, Vasantha. University of Michigan; Estados Unidos
Materia
ADIPOSE TISSUE
GROWTH
INSULIN
LEPTIN
OVARY
STEROIDOGENESIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/110986
Acceder
id CONICETDig_ce9e85a51bb79a208fd8ebd9d875243b
oai_identifier_str oai:ri.conicet.gov.ar:11336/110986
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Developmental Programming: Does Prenatal Steroid Excess Disrupt the Ovarian VEGF System in Sheep?1Ortega, Hugo HectorVeiga-Lopez, A.Sreedharan, S.Velázquez, Melisa María del LujánSalvetti, Natalia RaquelPadmanabhan, VasanthaADIPOSE TISSUEGROWTHINSULINLEPTINOVARYSTEROIDOGENESIShttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3Prenatal testosterone (T), but not dihydrotestosterone (DHT), excess disrupts ovarian cyclicity and increases follicular recruitment and persistence. We hypothesized that the disruption in the vascular endothelial growth factor (VEGF) system contributes to the enhancement of follicular recruitment and persistence in prenatal T-treated sheep. Impact of T / DHT treatments from days 30-90 of gestation on VEGFA, VEGFB, and their receptor (VEGFR-1 (FLT1), VEGFR-2 (KDR), and VEGFR-3 (FLT4)) protein expression was examined by immunohistochemistry on fetal day 90, 140, 22 weeks, 10 months (postpubertal), and 21 months (adult) of age. Arterial morphometry was performed in fetal day 140 and postpubertal ovaries. VEGFA and VEGFB expression were found in granulosa cells at all stages of follicular development with increased expression in antral follicles. VEGFA was present in theca interna, while VEGFB was present in theca interna / externa, and stromal cells. All 3 receptors were expressed in the granulosa, theca, and stromal cells during all stages of follicular development. VEGFR-3 increased with follicular differentiation with the highest level seen in the granulosa cells of antral follicles. None of the members of the VEGF family or their receptor expression were altered by age, or prenatal T / DHT treatments. At fetal day 140, area, wall thickness, and wall area of arteries from the ovarian hilum were larger in prenatal T- and DHT-treated females, suggestive of early androgenic programming of arterial differentiation. This may facilitate increased delivery of endocrine factors and thus indirectly contribute to the development of multifollicular phenotype.Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Veiga-Lopez, A.. University of Michigan; Estados UnidosFil: Sreedharan, S.. University of Michigan; Estados UnidosFil: Velázquez, Melisa María del Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Salvetti, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Padmanabhan, Vasantha. University of Michigan; Estados UnidosSociety for the Study of Reproduction2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/110986Ortega, Hugo Hector; Veiga-Lopez, A.; Sreedharan, S.; Velázquez, Melisa María del Luján; Salvetti, Natalia Raquel; et al.; Developmental Programming: Does Prenatal Steroid Excess Disrupt the Ovarian VEGF System in Sheep?1; Society for the Study of Reproduction; Biology of Reproduction; 93; 3; 10-2015; 58-650006-3363CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1095/biolreprod.115.131607info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:52Zoai:ri.conicet.gov.ar:11336/110986instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:53.277CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Developmental Programming: Does Prenatal Steroid Excess Disrupt the Ovarian VEGF System in Sheep?1
title Developmental Programming: Does Prenatal Steroid Excess Disrupt the Ovarian VEGF System in Sheep?1
spellingShingle Developmental Programming: Does Prenatal Steroid Excess Disrupt the Ovarian VEGF System in Sheep?1
Ortega, Hugo Hector
ADIPOSE TISSUE
GROWTH
INSULIN
LEPTIN
OVARY
STEROIDOGENESIS
title_short Developmental Programming: Does Prenatal Steroid Excess Disrupt the Ovarian VEGF System in Sheep?1
title_full Developmental Programming: Does Prenatal Steroid Excess Disrupt the Ovarian VEGF System in Sheep?1
title_fullStr Developmental Programming: Does Prenatal Steroid Excess Disrupt the Ovarian VEGF System in Sheep?1
title_full_unstemmed Developmental Programming: Does Prenatal Steroid Excess Disrupt the Ovarian VEGF System in Sheep?1
title_sort Developmental Programming: Does Prenatal Steroid Excess Disrupt the Ovarian VEGF System in Sheep?1
dc.creator.none.fl_str_mv Ortega, Hugo Hector
Veiga-Lopez, A.
Sreedharan, S.
Velázquez, Melisa María del Luján
Salvetti, Natalia Raquel
Padmanabhan, Vasantha
author Ortega, Hugo Hector
author_facet Ortega, Hugo Hector
Veiga-Lopez, A.
Sreedharan, S.
Velázquez, Melisa María del Luján
Salvetti, Natalia Raquel
Padmanabhan, Vasantha
author_role author
author2 Veiga-Lopez, A.
Sreedharan, S.
Velázquez, Melisa María del Luján
Salvetti, Natalia Raquel
Padmanabhan, Vasantha
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ADIPOSE TISSUE
GROWTH
INSULIN
LEPTIN
OVARY
STEROIDOGENESIS
topic ADIPOSE TISSUE
GROWTH
INSULIN
LEPTIN
OVARY
STEROIDOGENESIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Prenatal testosterone (T), but not dihydrotestosterone (DHT), excess disrupts ovarian cyclicity and increases follicular recruitment and persistence. We hypothesized that the disruption in the vascular endothelial growth factor (VEGF) system contributes to the enhancement of follicular recruitment and persistence in prenatal T-treated sheep. Impact of T / DHT treatments from days 30-90 of gestation on VEGFA, VEGFB, and their receptor (VEGFR-1 (FLT1), VEGFR-2 (KDR), and VEGFR-3 (FLT4)) protein expression was examined by immunohistochemistry on fetal day 90, 140, 22 weeks, 10 months (postpubertal), and 21 months (adult) of age. Arterial morphometry was performed in fetal day 140 and postpubertal ovaries. VEGFA and VEGFB expression were found in granulosa cells at all stages of follicular development with increased expression in antral follicles. VEGFA was present in theca interna, while VEGFB was present in theca interna / externa, and stromal cells. All 3 receptors were expressed in the granulosa, theca, and stromal cells during all stages of follicular development. VEGFR-3 increased with follicular differentiation with the highest level seen in the granulosa cells of antral follicles. None of the members of the VEGF family or their receptor expression were altered by age, or prenatal T / DHT treatments. At fetal day 140, area, wall thickness, and wall area of arteries from the ovarian hilum were larger in prenatal T- and DHT-treated females, suggestive of early androgenic programming of arterial differentiation. This may facilitate increased delivery of endocrine factors and thus indirectly contribute to the development of multifollicular phenotype.
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Veiga-Lopez, A.. University of Michigan; Estados Unidos
Fil: Sreedharan, S.. University of Michigan; Estados Unidos
Fil: Velázquez, Melisa María del Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Salvetti, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Padmanabhan, Vasantha. University of Michigan; Estados Unidos
description Prenatal testosterone (T), but not dihydrotestosterone (DHT), excess disrupts ovarian cyclicity and increases follicular recruitment and persistence. We hypothesized that the disruption in the vascular endothelial growth factor (VEGF) system contributes to the enhancement of follicular recruitment and persistence in prenatal T-treated sheep. Impact of T / DHT treatments from days 30-90 of gestation on VEGFA, VEGFB, and their receptor (VEGFR-1 (FLT1), VEGFR-2 (KDR), and VEGFR-3 (FLT4)) protein expression was examined by immunohistochemistry on fetal day 90, 140, 22 weeks, 10 months (postpubertal), and 21 months (adult) of age. Arterial morphometry was performed in fetal day 140 and postpubertal ovaries. VEGFA and VEGFB expression were found in granulosa cells at all stages of follicular development with increased expression in antral follicles. VEGFA was present in theca interna, while VEGFB was present in theca interna / externa, and stromal cells. All 3 receptors were expressed in the granulosa, theca, and stromal cells during all stages of follicular development. VEGFR-3 increased with follicular differentiation with the highest level seen in the granulosa cells of antral follicles. None of the members of the VEGF family or their receptor expression were altered by age, or prenatal T / DHT treatments. At fetal day 140, area, wall thickness, and wall area of arteries from the ovarian hilum were larger in prenatal T- and DHT-treated females, suggestive of early androgenic programming of arterial differentiation. This may facilitate increased delivery of endocrine factors and thus indirectly contribute to the development of multifollicular phenotype.
publishDate 2015
dc.date.none.fl_str_mv 2015-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/110986
Ortega, Hugo Hector; Veiga-Lopez, A.; Sreedharan, S.; Velázquez, Melisa María del Luján; Salvetti, Natalia Raquel; et al.; Developmental Programming: Does Prenatal Steroid Excess Disrupt the Ovarian VEGF System in Sheep?1; Society for the Study of Reproduction; Biology of Reproduction; 93; 3; 10-2015; 58-65
0006-3363
CONICET Digital
CONICET
url http://hdl.handle.net/11336/110986
identifier_str_mv Ortega, Hugo Hector; Veiga-Lopez, A.; Sreedharan, S.; Velázquez, Melisa María del Luján; Salvetti, Natalia Raquel; et al.; Developmental Programming: Does Prenatal Steroid Excess Disrupt the Ovarian VEGF System in Sheep?1; Society for the Study of Reproduction; Biology of Reproduction; 93; 3; 10-2015; 58-65
0006-3363
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1095/biolreprod.115.131607
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society for the Study of Reproduction
publisher.none.fl_str_mv Society for the Study of Reproduction
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268944784687104
score 13.13397

Publicaciones similares