Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina
- Autores
- Suárez, O.V.; Cueto, G.R.; Cavia, R.; Villafañe, I.E.G.; Bilenca, D.N.; Edelstein, A.; Martínez, P.; Miguel, S.; Bellomo, C.; Hodara, K.; Padula, P.J.; Busch, M.
- Año de publicación
- 2003
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- We studied hantavirus seroprevalence and virus variability in rodent populations in Diego Gaynor, northwest of Buenos Aires province, Argentina. Rodent samplings were conducted in railroads and cropfield borders in March and July 1999, September and December 2000, and March 2001. Antibody detection was performed by an enzyme link immunosorbent assay (ELISA), using the recombinant nucleoprotein of Andes (AND) virus as antigen. Tissue samples were taken from positive antibody individuals in order to confirm the presence of hantavirus genomic material and to identify virus genotypes. Akodon azarae was the most abundant species, followed by Oligoryzomys flavescens, while Calomys laucha and C. musculinus were rarely caught. We found a rate of seroprevalence of 9.3% for a total sample of 291 A. azarae and 13.5% for 37 O. flavescens. After molecular analyses of hantavirus, we confirmed the presence of hantavirus genomic material in 16 individuals with ELISA (+) results and two individuals with ELISA (-). Four amplimers for each species were sequenced and compared to the corresponding sequences of representative hantaviruses. We identified the AND Cent Lee from three O. flavescens, and the Pergamino virus from four A. azarae and from one O. flavescens. A. azarae males had higher seroprevalence than females, and heavier individuals showed higher seroprevalence than lighter ones. We did not find seroprevalence differences according to sex in O. flavescens, although this result may have been produced by the low sample size. The lowest seroprevalence was found in a period of high rodent density, when juveniles prevailed in the population. We found higher seroprevalences than those detected in previous studies for other localities of central Argentina where cases of hantavirus pulmonary syndrome (HPS) have been reported. The presence of AND Cent Lee virus in rodent populations of the study area, which is responsible of HPS cases in central Argentina, suggests that human populations are at risk of HPS disease, although there were not reported cases of this disease until today.
Fil:Suárez, O.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Cueto, G.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Cavia, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Bilenca, D.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Edelstein, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Hodara, K. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Padula, P.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Busch, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. - Fuente
- Mem. Inst. Oswaldo Cruz 2003;98(6):727-732
- Materia
-
Argentina
Hantavirus pulmonary syndrome
Seroprevalence
Sigmodontine rodents
nucleoprotein
recombinant protein
virus antigen
virus protein
antibody detection
Argentina
article
enzyme linked immunosorbent assay
gene sequence
genotype
Hantavirus
Hantavirus pulmonary syndrome
histopathology
molecular biology
nonhuman
railway
rodent
seroprevalence
species comparison
virus genome
virus identification
Akodon
Akodon azarae
Andes virus
Calomys
Calomys laucha
Calomys musculinus
Convict Creek 107 virus
Hantaan virus Lee
Hantavirus
Oligoryzomys
Oligoryzomys flavescens
Pergamino virus
Rodentia - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/2.5/ar
- Repositorio
- Institución
- Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
- OAI Identificador
- paperaa:paper_00740276_v98_n6_p727_Suarez
Ver los metadatos del registro completo
id |
BDUBAFCEN_577262dd9f2f2b5da42ac65fe87fb343 |
---|---|
oai_identifier_str |
paperaa:paper_00740276_v98_n6_p727_Suarez |
network_acronym_str |
BDUBAFCEN |
repository_id_str |
1896 |
network_name_str |
Biblioteca Digital (UBA-FCEN) |
spelling |
Prevalence of Infection with Hantavirus in Rodent Populations of Central ArgentinaSuárez, O.V.Cueto, G.R.Cavia, R.Villafañe, I.E.G.Bilenca, D.N.Edelstein, A.Martínez, P.Miguel, S.Bellomo, C.Hodara, K.Padula, P.J.Busch, M.ArgentinaHantavirus pulmonary syndromeSeroprevalenceSigmodontine rodentsnucleoproteinrecombinant proteinvirus antigenvirus proteinantibody detectionArgentinaarticleenzyme linked immunosorbent assaygene sequencegenotypeHantavirusHantavirus pulmonary syndromehistopathologymolecular biologynonhumanrailwayrodentseroprevalencespecies comparisonvirus genomevirus identificationAkodonAkodon azaraeAndes virusCalomysCalomys lauchaCalomys musculinusConvict Creek 107 virusHantaan virus LeeHantavirusOligoryzomysOligoryzomys flavescensPergamino virusRodentiaWe studied hantavirus seroprevalence and virus variability in rodent populations in Diego Gaynor, northwest of Buenos Aires province, Argentina. Rodent samplings were conducted in railroads and cropfield borders in March and July 1999, September and December 2000, and March 2001. Antibody detection was performed by an enzyme link immunosorbent assay (ELISA), using the recombinant nucleoprotein of Andes (AND) virus as antigen. Tissue samples were taken from positive antibody individuals in order to confirm the presence of hantavirus genomic material and to identify virus genotypes. Akodon azarae was the most abundant species, followed by Oligoryzomys flavescens, while Calomys laucha and C. musculinus were rarely caught. We found a rate of seroprevalence of 9.3% for a total sample of 291 A. azarae and 13.5% for 37 O. flavescens. After molecular analyses of hantavirus, we confirmed the presence of hantavirus genomic material in 16 individuals with ELISA (+) results and two individuals with ELISA (-). Four amplimers for each species were sequenced and compared to the corresponding sequences of representative hantaviruses. We identified the AND Cent Lee from three O. flavescens, and the Pergamino virus from four A. azarae and from one O. flavescens. A. azarae males had higher seroprevalence than females, and heavier individuals showed higher seroprevalence than lighter ones. We did not find seroprevalence differences according to sex in O. flavescens, although this result may have been produced by the low sample size. The lowest seroprevalence was found in a period of high rodent density, when juveniles prevailed in the population. We found higher seroprevalences than those detected in previous studies for other localities of central Argentina where cases of hantavirus pulmonary syndrome (HPS) have been reported. The presence of AND Cent Lee virus in rodent populations of the study area, which is responsible of HPS cases in central Argentina, suggests that human populations are at risk of HPS disease, although there were not reported cases of this disease until today.Fil:Suárez, O.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Cueto, G.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Cavia, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Bilenca, D.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Edelstein, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Hodara, K. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Padula, P.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Busch, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2003info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00740276_v98_n6_p727_SuarezMem. Inst. Oswaldo Cruz 2003;98(6):727-732reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-10-23T11:18:15Zpaperaa:paper_00740276_v98_n6_p727_SuarezInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-10-23 11:18:17.094Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse |
dc.title.none.fl_str_mv |
Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina |
title |
Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina |
spellingShingle |
Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina Suárez, O.V. Argentina Hantavirus pulmonary syndrome Seroprevalence Sigmodontine rodents nucleoprotein recombinant protein virus antigen virus protein antibody detection Argentina article enzyme linked immunosorbent assay gene sequence genotype Hantavirus Hantavirus pulmonary syndrome histopathology molecular biology nonhuman railway rodent seroprevalence species comparison virus genome virus identification Akodon Akodon azarae Andes virus Calomys Calomys laucha Calomys musculinus Convict Creek 107 virus Hantaan virus Lee Hantavirus Oligoryzomys Oligoryzomys flavescens Pergamino virus Rodentia |
title_short |
Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina |
title_full |
Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina |
title_fullStr |
Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina |
title_full_unstemmed |
Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina |
title_sort |
Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina |
dc.creator.none.fl_str_mv |
Suárez, O.V. Cueto, G.R. Cavia, R. Villafañe, I.E.G. Bilenca, D.N. Edelstein, A. Martínez, P. Miguel, S. Bellomo, C. Hodara, K. Padula, P.J. Busch, M. |
author |
Suárez, O.V. |
author_facet |
Suárez, O.V. Cueto, G.R. Cavia, R. Villafañe, I.E.G. Bilenca, D.N. Edelstein, A. Martínez, P. Miguel, S. Bellomo, C. Hodara, K. Padula, P.J. Busch, M. |
author_role |
author |
author2 |
Cueto, G.R. Cavia, R. Villafañe, I.E.G. Bilenca, D.N. Edelstein, A. Martínez, P. Miguel, S. Bellomo, C. Hodara, K. Padula, P.J. Busch, M. |
author2_role |
author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Argentina Hantavirus pulmonary syndrome Seroprevalence Sigmodontine rodents nucleoprotein recombinant protein virus antigen virus protein antibody detection Argentina article enzyme linked immunosorbent assay gene sequence genotype Hantavirus Hantavirus pulmonary syndrome histopathology molecular biology nonhuman railway rodent seroprevalence species comparison virus genome virus identification Akodon Akodon azarae Andes virus Calomys Calomys laucha Calomys musculinus Convict Creek 107 virus Hantaan virus Lee Hantavirus Oligoryzomys Oligoryzomys flavescens Pergamino virus Rodentia |
topic |
Argentina Hantavirus pulmonary syndrome Seroprevalence Sigmodontine rodents nucleoprotein recombinant protein virus antigen virus protein antibody detection Argentina article enzyme linked immunosorbent assay gene sequence genotype Hantavirus Hantavirus pulmonary syndrome histopathology molecular biology nonhuman railway rodent seroprevalence species comparison virus genome virus identification Akodon Akodon azarae Andes virus Calomys Calomys laucha Calomys musculinus Convict Creek 107 virus Hantaan virus Lee Hantavirus Oligoryzomys Oligoryzomys flavescens Pergamino virus Rodentia |
dc.description.none.fl_txt_mv |
We studied hantavirus seroprevalence and virus variability in rodent populations in Diego Gaynor, northwest of Buenos Aires province, Argentina. Rodent samplings were conducted in railroads and cropfield borders in March and July 1999, September and December 2000, and March 2001. Antibody detection was performed by an enzyme link immunosorbent assay (ELISA), using the recombinant nucleoprotein of Andes (AND) virus as antigen. Tissue samples were taken from positive antibody individuals in order to confirm the presence of hantavirus genomic material and to identify virus genotypes. Akodon azarae was the most abundant species, followed by Oligoryzomys flavescens, while Calomys laucha and C. musculinus were rarely caught. We found a rate of seroprevalence of 9.3% for a total sample of 291 A. azarae and 13.5% for 37 O. flavescens. After molecular analyses of hantavirus, we confirmed the presence of hantavirus genomic material in 16 individuals with ELISA (+) results and two individuals with ELISA (-). Four amplimers for each species were sequenced and compared to the corresponding sequences of representative hantaviruses. We identified the AND Cent Lee from three O. flavescens, and the Pergamino virus from four A. azarae and from one O. flavescens. A. azarae males had higher seroprevalence than females, and heavier individuals showed higher seroprevalence than lighter ones. We did not find seroprevalence differences according to sex in O. flavescens, although this result may have been produced by the low sample size. The lowest seroprevalence was found in a period of high rodent density, when juveniles prevailed in the population. We found higher seroprevalences than those detected in previous studies for other localities of central Argentina where cases of hantavirus pulmonary syndrome (HPS) have been reported. The presence of AND Cent Lee virus in rodent populations of the study area, which is responsible of HPS cases in central Argentina, suggests that human populations are at risk of HPS disease, although there were not reported cases of this disease until today. Fil:Suárez, O.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cueto, G.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cavia, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bilenca, D.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Edelstein, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hodara, K. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Padula, P.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Busch, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. |
description |
We studied hantavirus seroprevalence and virus variability in rodent populations in Diego Gaynor, northwest of Buenos Aires province, Argentina. Rodent samplings were conducted in railroads and cropfield borders in March and July 1999, September and December 2000, and March 2001. Antibody detection was performed by an enzyme link immunosorbent assay (ELISA), using the recombinant nucleoprotein of Andes (AND) virus as antigen. Tissue samples were taken from positive antibody individuals in order to confirm the presence of hantavirus genomic material and to identify virus genotypes. Akodon azarae was the most abundant species, followed by Oligoryzomys flavescens, while Calomys laucha and C. musculinus were rarely caught. We found a rate of seroprevalence of 9.3% for a total sample of 291 A. azarae and 13.5% for 37 O. flavescens. After molecular analyses of hantavirus, we confirmed the presence of hantavirus genomic material in 16 individuals with ELISA (+) results and two individuals with ELISA (-). Four amplimers for each species were sequenced and compared to the corresponding sequences of representative hantaviruses. We identified the AND Cent Lee from three O. flavescens, and the Pergamino virus from four A. azarae and from one O. flavescens. A. azarae males had higher seroprevalence than females, and heavier individuals showed higher seroprevalence than lighter ones. We did not find seroprevalence differences according to sex in O. flavescens, although this result may have been produced by the low sample size. The lowest seroprevalence was found in a period of high rodent density, when juveniles prevailed in the population. We found higher seroprevalences than those detected in previous studies for other localities of central Argentina where cases of hantavirus pulmonary syndrome (HPS) have been reported. The presence of AND Cent Lee virus in rodent populations of the study area, which is responsible of HPS cases in central Argentina, suggests that human populations are at risk of HPS disease, although there were not reported cases of this disease until today. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12110/paper_00740276_v98_n6_p727_Suarez |
url |
http://hdl.handle.net/20.500.12110/paper_00740276_v98_n6_p727_Suarez |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/2.5/ar |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Mem. Inst. Oswaldo Cruz 2003;98(6):727-732 reponame:Biblioteca Digital (UBA-FCEN) instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales instacron:UBA-FCEN |
reponame_str |
Biblioteca Digital (UBA-FCEN) |
collection |
Biblioteca Digital (UBA-FCEN) |
instname_str |
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
instacron_str |
UBA-FCEN |
institution |
UBA-FCEN |
repository.name.fl_str_mv |
Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
repository.mail.fl_str_mv |
ana@bl.fcen.uba.ar |
_version_ |
1846784876259311616 |
score |
12.6884985 |