Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina

Autores
Suárez, O.V.; Cueto, G.R.; Cavia, R.; Villafañe, I.E.G.; Bilenca, D.N.; Edelstein, A.; Martínez, P.; Miguel, S.; Bellomo, C.; Hodara, K.; Padula, P.J.; Busch, M.
Año de publicación
2003
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We studied hantavirus seroprevalence and virus variability in rodent populations in Diego Gaynor, northwest of Buenos Aires province, Argentina. Rodent samplings were conducted in railroads and cropfield borders in March and July 1999, September and December 2000, and March 2001. Antibody detection was performed by an enzyme link immunosorbent assay (ELISA), using the recombinant nucleoprotein of Andes (AND) virus as antigen. Tissue samples were taken from positive antibody individuals in order to confirm the presence of hantavirus genomic material and to identify virus genotypes. Akodon azarae was the most abundant species, followed by Oligoryzomys flavescens, while Calomys laucha and C. musculinus were rarely caught. We found a rate of seroprevalence of 9.3% for a total sample of 291 A. azarae and 13.5% for 37 O. flavescens. After molecular analyses of hantavirus, we confirmed the presence of hantavirus genomic material in 16 individuals with ELISA (+) results and two individuals with ELISA (-). Four amplimers for each species were sequenced and compared to the corresponding sequences of representative hantaviruses. We identified the AND Cent Lee from three O. flavescens, and the Pergamino virus from four A. azarae and from one O. flavescens. A. azarae males had higher seroprevalence than females, and heavier individuals showed higher seroprevalence than lighter ones. We did not find seroprevalence differences according to sex in O. flavescens, although this result may have been produced by the low sample size. The lowest seroprevalence was found in a period of high rodent density, when juveniles prevailed in the population. We found higher seroprevalences than those detected in previous studies for other localities of central Argentina where cases of hantavirus pulmonary syndrome (HPS) have been reported. The presence of AND Cent Lee virus in rodent populations of the study area, which is responsible of HPS cases in central Argentina, suggests that human populations are at risk of HPS disease, although there were not reported cases of this disease until today.
Fil:Suárez, O.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Cueto, G.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Cavia, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Bilenca, D.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Edelstein, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Hodara, K. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Padula, P.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Busch, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
Mem. Inst. Oswaldo Cruz 2003;98(6):727-732
Materia
Argentina
Hantavirus pulmonary syndrome
Seroprevalence
Sigmodontine rodents
nucleoprotein
recombinant protein
virus antigen
virus protein
antibody detection
Argentina
article
enzyme linked immunosorbent assay
gene sequence
genotype
Hantavirus
Hantavirus pulmonary syndrome
histopathology
molecular biology
nonhuman
railway
rodent
seroprevalence
species comparison
virus genome
virus identification
Akodon
Akodon azarae
Andes virus
Calomys
Calomys laucha
Calomys musculinus
Convict Creek 107 virus
Hantaan virus Lee
Hantavirus
Oligoryzomys
Oligoryzomys flavescens
Pergamino virus
Rodentia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_00740276_v98_n6_p727_Suarez

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oai_identifier_str paperaa:paper_00740276_v98_n6_p727_Suarez
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Prevalence of Infection with Hantavirus in Rodent Populations of Central ArgentinaSuárez, O.V.Cueto, G.R.Cavia, R.Villafañe, I.E.G.Bilenca, D.N.Edelstein, A.Martínez, P.Miguel, S.Bellomo, C.Hodara, K.Padula, P.J.Busch, M.ArgentinaHantavirus pulmonary syndromeSeroprevalenceSigmodontine rodentsnucleoproteinrecombinant proteinvirus antigenvirus proteinantibody detectionArgentinaarticleenzyme linked immunosorbent assaygene sequencegenotypeHantavirusHantavirus pulmonary syndromehistopathologymolecular biologynonhumanrailwayrodentseroprevalencespecies comparisonvirus genomevirus identificationAkodonAkodon azaraeAndes virusCalomysCalomys lauchaCalomys musculinusConvict Creek 107 virusHantaan virus LeeHantavirusOligoryzomysOligoryzomys flavescensPergamino virusRodentiaWe studied hantavirus seroprevalence and virus variability in rodent populations in Diego Gaynor, northwest of Buenos Aires province, Argentina. Rodent samplings were conducted in railroads and cropfield borders in March and July 1999, September and December 2000, and March 2001. Antibody detection was performed by an enzyme link immunosorbent assay (ELISA), using the recombinant nucleoprotein of Andes (AND) virus as antigen. Tissue samples were taken from positive antibody individuals in order to confirm the presence of hantavirus genomic material and to identify virus genotypes. Akodon azarae was the most abundant species, followed by Oligoryzomys flavescens, while Calomys laucha and C. musculinus were rarely caught. We found a rate of seroprevalence of 9.3% for a total sample of 291 A. azarae and 13.5% for 37 O. flavescens. After molecular analyses of hantavirus, we confirmed the presence of hantavirus genomic material in 16 individuals with ELISA (+) results and two individuals with ELISA (-). Four amplimers for each species were sequenced and compared to the corresponding sequences of representative hantaviruses. We identified the AND Cent Lee from three O. flavescens, and the Pergamino virus from four A. azarae and from one O. flavescens. A. azarae males had higher seroprevalence than females, and heavier individuals showed higher seroprevalence than lighter ones. We did not find seroprevalence differences according to sex in O. flavescens, although this result may have been produced by the low sample size. The lowest seroprevalence was found in a period of high rodent density, when juveniles prevailed in the population. We found higher seroprevalences than those detected in previous studies for other localities of central Argentina where cases of hantavirus pulmonary syndrome (HPS) have been reported. The presence of AND Cent Lee virus in rodent populations of the study area, which is responsible of HPS cases in central Argentina, suggests that human populations are at risk of HPS disease, although there were not reported cases of this disease until today.Fil:Suárez, O.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Cueto, G.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Cavia, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Bilenca, D.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Edelstein, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Hodara, K. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Padula, P.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Busch, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2003info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00740276_v98_n6_p727_SuarezMem. Inst. Oswaldo Cruz 2003;98(6):727-732reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-10-23T11:18:15Zpaperaa:paper_00740276_v98_n6_p727_SuarezInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-10-23 11:18:17.094Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina
title Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina
spellingShingle Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina
Suárez, O.V.
Argentina
Hantavirus pulmonary syndrome
Seroprevalence
Sigmodontine rodents
nucleoprotein
recombinant protein
virus antigen
virus protein
antibody detection
Argentina
article
enzyme linked immunosorbent assay
gene sequence
genotype
Hantavirus
Hantavirus pulmonary syndrome
histopathology
molecular biology
nonhuman
railway
rodent
seroprevalence
species comparison
virus genome
virus identification
Akodon
Akodon azarae
Andes virus
Calomys
Calomys laucha
Calomys musculinus
Convict Creek 107 virus
Hantaan virus Lee
Hantavirus
Oligoryzomys
Oligoryzomys flavescens
Pergamino virus
Rodentia
title_short Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina
title_full Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina
title_fullStr Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina
title_full_unstemmed Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina
title_sort Prevalence of Infection with Hantavirus in Rodent Populations of Central Argentina
dc.creator.none.fl_str_mv Suárez, O.V.
Cueto, G.R.
Cavia, R.
Villafañe, I.E.G.
Bilenca, D.N.
Edelstein, A.
Martínez, P.
Miguel, S.
Bellomo, C.
Hodara, K.
Padula, P.J.
Busch, M.
author Suárez, O.V.
author_facet Suárez, O.V.
Cueto, G.R.
Cavia, R.
Villafañe, I.E.G.
Bilenca, D.N.
Edelstein, A.
Martínez, P.
Miguel, S.
Bellomo, C.
Hodara, K.
Padula, P.J.
Busch, M.
author_role author
author2 Cueto, G.R.
Cavia, R.
Villafañe, I.E.G.
Bilenca, D.N.
Edelstein, A.
Martínez, P.
Miguel, S.
Bellomo, C.
Hodara, K.
Padula, P.J.
Busch, M.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Argentina
Hantavirus pulmonary syndrome
Seroprevalence
Sigmodontine rodents
nucleoprotein
recombinant protein
virus antigen
virus protein
antibody detection
Argentina
article
enzyme linked immunosorbent assay
gene sequence
genotype
Hantavirus
Hantavirus pulmonary syndrome
histopathology
molecular biology
nonhuman
railway
rodent
seroprevalence
species comparison
virus genome
virus identification
Akodon
Akodon azarae
Andes virus
Calomys
Calomys laucha
Calomys musculinus
Convict Creek 107 virus
Hantaan virus Lee
Hantavirus
Oligoryzomys
Oligoryzomys flavescens
Pergamino virus
Rodentia
topic Argentina
Hantavirus pulmonary syndrome
Seroprevalence
Sigmodontine rodents
nucleoprotein
recombinant protein
virus antigen
virus protein
antibody detection
Argentina
article
enzyme linked immunosorbent assay
gene sequence
genotype
Hantavirus
Hantavirus pulmonary syndrome
histopathology
molecular biology
nonhuman
railway
rodent
seroprevalence
species comparison
virus genome
virus identification
Akodon
Akodon azarae
Andes virus
Calomys
Calomys laucha
Calomys musculinus
Convict Creek 107 virus
Hantaan virus Lee
Hantavirus
Oligoryzomys
Oligoryzomys flavescens
Pergamino virus
Rodentia
dc.description.none.fl_txt_mv We studied hantavirus seroprevalence and virus variability in rodent populations in Diego Gaynor, northwest of Buenos Aires province, Argentina. Rodent samplings were conducted in railroads and cropfield borders in March and July 1999, September and December 2000, and March 2001. Antibody detection was performed by an enzyme link immunosorbent assay (ELISA), using the recombinant nucleoprotein of Andes (AND) virus as antigen. Tissue samples were taken from positive antibody individuals in order to confirm the presence of hantavirus genomic material and to identify virus genotypes. Akodon azarae was the most abundant species, followed by Oligoryzomys flavescens, while Calomys laucha and C. musculinus were rarely caught. We found a rate of seroprevalence of 9.3% for a total sample of 291 A. azarae and 13.5% for 37 O. flavescens. After molecular analyses of hantavirus, we confirmed the presence of hantavirus genomic material in 16 individuals with ELISA (+) results and two individuals with ELISA (-). Four amplimers for each species were sequenced and compared to the corresponding sequences of representative hantaviruses. We identified the AND Cent Lee from three O. flavescens, and the Pergamino virus from four A. azarae and from one O. flavescens. A. azarae males had higher seroprevalence than females, and heavier individuals showed higher seroprevalence than lighter ones. We did not find seroprevalence differences according to sex in O. flavescens, although this result may have been produced by the low sample size. The lowest seroprevalence was found in a period of high rodent density, when juveniles prevailed in the population. We found higher seroprevalences than those detected in previous studies for other localities of central Argentina where cases of hantavirus pulmonary syndrome (HPS) have been reported. The presence of AND Cent Lee virus in rodent populations of the study area, which is responsible of HPS cases in central Argentina, suggests that human populations are at risk of HPS disease, although there were not reported cases of this disease until today.
Fil:Suárez, O.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Cueto, G.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Cavia, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Bilenca, D.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Edelstein, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Hodara, K. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Padula, P.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Busch, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description We studied hantavirus seroprevalence and virus variability in rodent populations in Diego Gaynor, northwest of Buenos Aires province, Argentina. Rodent samplings were conducted in railroads and cropfield borders in March and July 1999, September and December 2000, and March 2001. Antibody detection was performed by an enzyme link immunosorbent assay (ELISA), using the recombinant nucleoprotein of Andes (AND) virus as antigen. Tissue samples were taken from positive antibody individuals in order to confirm the presence of hantavirus genomic material and to identify virus genotypes. Akodon azarae was the most abundant species, followed by Oligoryzomys flavescens, while Calomys laucha and C. musculinus were rarely caught. We found a rate of seroprevalence of 9.3% for a total sample of 291 A. azarae and 13.5% for 37 O. flavescens. After molecular analyses of hantavirus, we confirmed the presence of hantavirus genomic material in 16 individuals with ELISA (+) results and two individuals with ELISA (-). Four amplimers for each species were sequenced and compared to the corresponding sequences of representative hantaviruses. We identified the AND Cent Lee from three O. flavescens, and the Pergamino virus from four A. azarae and from one O. flavescens. A. azarae males had higher seroprevalence than females, and heavier individuals showed higher seroprevalence than lighter ones. We did not find seroprevalence differences according to sex in O. flavescens, although this result may have been produced by the low sample size. The lowest seroprevalence was found in a period of high rodent density, when juveniles prevailed in the population. We found higher seroprevalences than those detected in previous studies for other localities of central Argentina where cases of hantavirus pulmonary syndrome (HPS) have been reported. The presence of AND Cent Lee virus in rodent populations of the study area, which is responsible of HPS cases in central Argentina, suggests that human populations are at risk of HPS disease, although there were not reported cases of this disease until today.
publishDate 2003
dc.date.none.fl_str_mv 2003
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_00740276_v98_n6_p727_Suarez
url http://hdl.handle.net/20.500.12110/paper_00740276_v98_n6_p727_Suarez
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Mem. Inst. Oswaldo Cruz 2003;98(6):727-732
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
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instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
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repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
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