Cyclosporine A from a nonpathogenic Fusarium oxysporum suppressing Sclerotinia sclerotiorum

• Autores: Rodríguez, M.A.; Cabrera, G.; Godeas, A.
• Año de publicación: 2006
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: J. Appl. Microbiol. 2006;100(3):575-586
• Materia: Antifungal activity; Biocontrol; Cyclosporine A; Fusarium oxysporum; Sclerotinia sclerotiorum; Soybean; cyclosporin A; fungicide; fungus; antifungal activity; article; chromatography; controlled study; correlation analysis; dilution; evaluation; fungal strain; fungus; fungus culture; Fusarium oxysporum; gene repression; greenhouse; growth inhibition; inoculation; metabolite; minimum inhibitory concentration; nonhuman; plant disease; purification; quantitative assay; sclerotinia sclerotiorum; soybean; spectroscopy; survival rate; Antifungal Agents; Ascomycota; Colony Count, Microbial; Culture Media; Cyclosporine; Food Microbiology; Fusarium; Microbial Sensitivity Tests; Pest Control, Biological; Soil Microbiology; Soybeans; Fungi; Fusarium oxysporum; Glycine max; Sclerotinia sclerotiorum
• Institución: Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales

Aims: To evaluate the antagonistic activity of Fusarium oxysporum nonpathogenic fungal strain S6 against the phytopathogenic fungus Sclerotinia sclerotiorum and to identify the antifungal compounds involved. Methods and Results: The antagonistic activity of Fusarium oxysporum strain S6 was determined in vitro by dual cultures. The metabolite responsible for the activity was isolated by chromatographic techniques, purified and identified by spectroscopic methods as cyclosporine A. The antifungal activity against the pathogen was correlated with the presence of this metabolite by a dilution assay and then quantified. Cyclosporine A caused both growth inhibition and suppression of sclerotia formation. In a greenhouse assay, a significant increase in the number of surviving soybean (Glycine max) plants was observed when S. sclerotiorum and F. oxysporum (S6) were inoculated together when compared with plants inoculated with S. sclerotiorum alone. Conclusion: Fusarium oxysporum (S6) may be a good fungal biological control agent for S. sclerotiorum and cyclosporine A is the responsible metabolite involved in its antagonistic activity in vitro. Significance and Impact of the Study: Cyclosporine A has not been previously described as an inhibitor of S. sclerotiorum. Its minimum inhibitory concentration (MIC) of 0·1 μg disc-1 makes it suitable to use as a biofungicide. In vivo experiments showed that F. oxysporum (S6) is a good candidate for the biocontrol of S. sclerotiorum in soybean. © 2006 The Society for Applied Microbiology.
Fil:Rodríguez, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Cabrera, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Godeas, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.