Occurrence of antimicrobial serin-proteinases in sunflower seeds
- Autores
- Mendieta, Julieta Renee; Giudici, Ana Marcela; de la Canal, Laura
- Año de publicación
- 2004
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Using an experimental approach directed to the isolation of antimicrobial proteins, we have detected the presence of a trypsin inhibitor (TI) with associated antifungal activity in sunflower seeds. Purification of the isolated protein by affinity chromatography on a trypsin-agarose matrix confirmed that a trypsin inhibitor was responsible for the inhibition of spore germination of the fungal pathogen Sclerotinia sclerotiorum. The protein is a potent antifungal compound as it can completely inhibit the germination of S. sclerotiorum ascospores at a concentration of 14 μg/ml. The putative contribution of this TI to control fungal invasion is discussed.
Fil: Mendieta, Julieta Renee. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Giudici, Ana Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: de la Canal, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina - Materia
-
Helianthus Annuus
Trypsin Inhibitor
Antifungal
Sclerotinia Sclerotiorum - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/30446
Ver los metadatos del registro completo
id |
CONICETDig_a2eff6681700f716a3d8e70efb1136e8 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/30446 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Occurrence of antimicrobial serin-proteinases in sunflower seedsMendieta, Julieta ReneeGiudici, Ana Marcelade la Canal, LauraHelianthus AnnuusTrypsin InhibitorAntifungalSclerotinia Sclerotiorumhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Using an experimental approach directed to the isolation of antimicrobial proteins, we have detected the presence of a trypsin inhibitor (TI) with associated antifungal activity in sunflower seeds. Purification of the isolated protein by affinity chromatography on a trypsin-agarose matrix confirmed that a trypsin inhibitor was responsible for the inhibition of spore germination of the fungal pathogen Sclerotinia sclerotiorum. The protein is a potent antifungal compound as it can completely inhibit the germination of S. sclerotiorum ascospores at a concentration of 14 μg/ml. The putative contribution of this TI to control fungal invasion is discussed.Fil: Mendieta, Julieta Renee. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Giudici, Ana Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: de la Canal, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaWiley Blackwell Publishing, Inc2004-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30446Mendieta, Julieta Renee; Giudici, Ana Marcela; de la Canal, Laura; Occurrence of antimicrobial serin-proteinases in sunflower seeds; Wiley Blackwell Publishing, Inc; Journal of Phytopathology-Phytopathologische Zeitschrift; 152; 6-2004; 43-470931-1785CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1046/j.1439-0434.2003.00799.xinfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1046/j.1439-0434.2003.00799.x/abstract;jsessionid=47D59F18CDC907A1D8D331A762A484A8.f04t03info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:53Zoai:ri.conicet.gov.ar:11336/30446instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:53.738CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Occurrence of antimicrobial serin-proteinases in sunflower seeds |
title |
Occurrence of antimicrobial serin-proteinases in sunflower seeds |
spellingShingle |
Occurrence of antimicrobial serin-proteinases in sunflower seeds Mendieta, Julieta Renee Helianthus Annuus Trypsin Inhibitor Antifungal Sclerotinia Sclerotiorum |
title_short |
Occurrence of antimicrobial serin-proteinases in sunflower seeds |
title_full |
Occurrence of antimicrobial serin-proteinases in sunflower seeds |
title_fullStr |
Occurrence of antimicrobial serin-proteinases in sunflower seeds |
title_full_unstemmed |
Occurrence of antimicrobial serin-proteinases in sunflower seeds |
title_sort |
Occurrence of antimicrobial serin-proteinases in sunflower seeds |
dc.creator.none.fl_str_mv |
Mendieta, Julieta Renee Giudici, Ana Marcela de la Canal, Laura |
author |
Mendieta, Julieta Renee |
author_facet |
Mendieta, Julieta Renee Giudici, Ana Marcela de la Canal, Laura |
author_role |
author |
author2 |
Giudici, Ana Marcela de la Canal, Laura |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Helianthus Annuus Trypsin Inhibitor Antifungal Sclerotinia Sclerotiorum |
topic |
Helianthus Annuus Trypsin Inhibitor Antifungal Sclerotinia Sclerotiorum |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Using an experimental approach directed to the isolation of antimicrobial proteins, we have detected the presence of a trypsin inhibitor (TI) with associated antifungal activity in sunflower seeds. Purification of the isolated protein by affinity chromatography on a trypsin-agarose matrix confirmed that a trypsin inhibitor was responsible for the inhibition of spore germination of the fungal pathogen Sclerotinia sclerotiorum. The protein is a potent antifungal compound as it can completely inhibit the germination of S. sclerotiorum ascospores at a concentration of 14 μg/ml. The putative contribution of this TI to control fungal invasion is discussed. Fil: Mendieta, Julieta Renee. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina Fil: Giudici, Ana Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina Fil: de la Canal, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina |
description |
Using an experimental approach directed to the isolation of antimicrobial proteins, we have detected the presence of a trypsin inhibitor (TI) with associated antifungal activity in sunflower seeds. Purification of the isolated protein by affinity chromatography on a trypsin-agarose matrix confirmed that a trypsin inhibitor was responsible for the inhibition of spore germination of the fungal pathogen Sclerotinia sclerotiorum. The protein is a potent antifungal compound as it can completely inhibit the germination of S. sclerotiorum ascospores at a concentration of 14 μg/ml. The putative contribution of this TI to control fungal invasion is discussed. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/30446 Mendieta, Julieta Renee; Giudici, Ana Marcela; de la Canal, Laura; Occurrence of antimicrobial serin-proteinases in sunflower seeds; Wiley Blackwell Publishing, Inc; Journal of Phytopathology-Phytopathologische Zeitschrift; 152; 6-2004; 43-47 0931-1785 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/30446 |
identifier_str_mv |
Mendieta, Julieta Renee; Giudici, Ana Marcela; de la Canal, Laura; Occurrence of antimicrobial serin-proteinases in sunflower seeds; Wiley Blackwell Publishing, Inc; Journal of Phytopathology-Phytopathologische Zeitschrift; 152; 6-2004; 43-47 0931-1785 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1046/j.1439-0434.2003.00799.x info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1046/j.1439-0434.2003.00799.x/abstract;jsessionid=47D59F18CDC907A1D8D331A762A484A8.f04t03 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
publisher.none.fl_str_mv |
Wiley Blackwell Publishing, Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613516748652544 |
score |
13.070432 |