Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and its sequence data
- Autores
- Gottlieb, A.M.; Giberti, G.C.; Poggio, L.
- Año de publicación
- 2005
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In order to clarify the relationships among southern South American (sSA) representatives of the genus Ilex, an amplified fragment length polymorphism (AFLP) analysis was accomplished. In addition, the phylogenetic relationships of the species were studied using ribosomal internal transcribed spacer (ITS) sequence data alone and in combination with AFLP data, taking into account the possible existence of paralogous sequences and the influence of alignment parameters. To explore stability of phylogenetic hypotheses, a sensitivity analysis was performed using 15 indel-substitution models. Within each species assayed, the AFLPs allowed the recognition of several diagnostic bands. Furthermore, the AFLP analysis revealed that individuals belonging to the same morpho-species formed coherent clades. In addition, some cases of geographical association were noted. Studies on ITS sequences revealed divergence between data obtained herein and sequence data downloaded from GenBank. The sensitivity analyses yielded different interspecific hypotheses of relationships. Notwithstanding, analyses of the ITS data alone and in combination with AFLPs, rendered clades stable to variation in the analytical parameters. Topologies obtained for the AFLPs, the ITS data alone and the combined analyses, demonstrated the existence of a group formed by I. argentina, I. brasiliensis, I. brevicuspis, I. integerrima, and I. theezans, and that I. dumosa and I. paraguariensis were distantly related to the former. Incongruence with traditional taxonomical treatments was found.
Fil:Gottlieb, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Poggio, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. - Fuente
- Am. J. Bot. 2005;92(2):352-369
- Materia
-
AFLP
Direct optimization
Ilex
ITS
Sensitivity analysis
molecular analysis
sensitivity analysis
taxonomy
Aquifoliaceae
Embryophyta
Ilex
Ilex
Ilex argentina
Ilex argentina
Ilex brasiliensis
Ilex brasiliensis
Ilex brevicuspis
Ilex brevicuspis
Ilex dumosa
Ilex dumosa
Ilex integerrima
Ilex integerrima
Ilex paraguariensis
Ilex paraguariensis
Ilex theezans
Ilex theezans - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/2.5/ar
- Repositorio
- Institución
- Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
- OAI Identificador
- paperaa:paper_00029122_v92_n2_p352_Gottlieb
Ver los metadatos del registro completo
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Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and its sequence dataGottlieb, A.M.Giberti, G.C.Poggio, L.AFLPDirect optimizationIlexITSSensitivity analysismolecular analysissensitivity analysistaxonomyAquifoliaceaeEmbryophytaIlexIlexIlex argentinaIlex argentinaIlex brasiliensisIlex brasiliensisIlex brevicuspisIlex brevicuspisIlex dumosaIlex dumosaIlex integerrimaIlex integerrimaIlex paraguariensisIlex paraguariensisIlex theezansIlex theezansIn order to clarify the relationships among southern South American (sSA) representatives of the genus Ilex, an amplified fragment length polymorphism (AFLP) analysis was accomplished. In addition, the phylogenetic relationships of the species were studied using ribosomal internal transcribed spacer (ITS) sequence data alone and in combination with AFLP data, taking into account the possible existence of paralogous sequences and the influence of alignment parameters. To explore stability of phylogenetic hypotheses, a sensitivity analysis was performed using 15 indel-substitution models. Within each species assayed, the AFLPs allowed the recognition of several diagnostic bands. Furthermore, the AFLP analysis revealed that individuals belonging to the same morpho-species formed coherent clades. In addition, some cases of geographical association were noted. Studies on ITS sequences revealed divergence between data obtained herein and sequence data downloaded from GenBank. The sensitivity analyses yielded different interspecific hypotheses of relationships. Notwithstanding, analyses of the ITS data alone and in combination with AFLPs, rendered clades stable to variation in the analytical parameters. Topologies obtained for the AFLPs, the ITS data alone and the combined analyses, demonstrated the existence of a group formed by I. argentina, I. brasiliensis, I. brevicuspis, I. integerrima, and I. theezans, and that I. dumosa and I. paraguariensis were distantly related to the former. Incongruence with traditional taxonomical treatments was found.Fil:Gottlieb, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Poggio, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2005info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00029122_v92_n2_p352_GottliebAm. J. Bot. 2005;92(2):352-369reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:43:01Zpaperaa:paper_00029122_v92_n2_p352_GottliebInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:43:01.992Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse |
dc.title.none.fl_str_mv |
Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and its sequence data |
title |
Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and its sequence data |
spellingShingle |
Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and its sequence data Gottlieb, A.M. AFLP Direct optimization Ilex ITS Sensitivity analysis molecular analysis sensitivity analysis taxonomy Aquifoliaceae Embryophyta Ilex Ilex Ilex argentina Ilex argentina Ilex brasiliensis Ilex brasiliensis Ilex brevicuspis Ilex brevicuspis Ilex dumosa Ilex dumosa Ilex integerrima Ilex integerrima Ilex paraguariensis Ilex paraguariensis Ilex theezans Ilex theezans |
title_short |
Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and its sequence data |
title_full |
Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and its sequence data |
title_fullStr |
Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and its sequence data |
title_full_unstemmed |
Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and its sequence data |
title_sort |
Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and its sequence data |
dc.creator.none.fl_str_mv |
Gottlieb, A.M. Giberti, G.C. Poggio, L. |
author |
Gottlieb, A.M. |
author_facet |
Gottlieb, A.M. Giberti, G.C. Poggio, L. |
author_role |
author |
author2 |
Giberti, G.C. Poggio, L. |
author2_role |
author author |
dc.subject.none.fl_str_mv |
AFLP Direct optimization Ilex ITS Sensitivity analysis molecular analysis sensitivity analysis taxonomy Aquifoliaceae Embryophyta Ilex Ilex Ilex argentina Ilex argentina Ilex brasiliensis Ilex brasiliensis Ilex brevicuspis Ilex brevicuspis Ilex dumosa Ilex dumosa Ilex integerrima Ilex integerrima Ilex paraguariensis Ilex paraguariensis Ilex theezans Ilex theezans |
topic |
AFLP Direct optimization Ilex ITS Sensitivity analysis molecular analysis sensitivity analysis taxonomy Aquifoliaceae Embryophyta Ilex Ilex Ilex argentina Ilex argentina Ilex brasiliensis Ilex brasiliensis Ilex brevicuspis Ilex brevicuspis Ilex dumosa Ilex dumosa Ilex integerrima Ilex integerrima Ilex paraguariensis Ilex paraguariensis Ilex theezans Ilex theezans |
dc.description.none.fl_txt_mv |
In order to clarify the relationships among southern South American (sSA) representatives of the genus Ilex, an amplified fragment length polymorphism (AFLP) analysis was accomplished. In addition, the phylogenetic relationships of the species were studied using ribosomal internal transcribed spacer (ITS) sequence data alone and in combination with AFLP data, taking into account the possible existence of paralogous sequences and the influence of alignment parameters. To explore stability of phylogenetic hypotheses, a sensitivity analysis was performed using 15 indel-substitution models. Within each species assayed, the AFLPs allowed the recognition of several diagnostic bands. Furthermore, the AFLP analysis revealed that individuals belonging to the same morpho-species formed coherent clades. In addition, some cases of geographical association were noted. Studies on ITS sequences revealed divergence between data obtained herein and sequence data downloaded from GenBank. The sensitivity analyses yielded different interspecific hypotheses of relationships. Notwithstanding, analyses of the ITS data alone and in combination with AFLPs, rendered clades stable to variation in the analytical parameters. Topologies obtained for the AFLPs, the ITS data alone and the combined analyses, demonstrated the existence of a group formed by I. argentina, I. brasiliensis, I. brevicuspis, I. integerrima, and I. theezans, and that I. dumosa and I. paraguariensis were distantly related to the former. Incongruence with traditional taxonomical treatments was found. Fil:Gottlieb, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Poggio, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. |
description |
In order to clarify the relationships among southern South American (sSA) representatives of the genus Ilex, an amplified fragment length polymorphism (AFLP) analysis was accomplished. In addition, the phylogenetic relationships of the species were studied using ribosomal internal transcribed spacer (ITS) sequence data alone and in combination with AFLP data, taking into account the possible existence of paralogous sequences and the influence of alignment parameters. To explore stability of phylogenetic hypotheses, a sensitivity analysis was performed using 15 indel-substitution models. Within each species assayed, the AFLPs allowed the recognition of several diagnostic bands. Furthermore, the AFLP analysis revealed that individuals belonging to the same morpho-species formed coherent clades. In addition, some cases of geographical association were noted. Studies on ITS sequences revealed divergence between data obtained herein and sequence data downloaded from GenBank. The sensitivity analyses yielded different interspecific hypotheses of relationships. Notwithstanding, analyses of the ITS data alone and in combination with AFLPs, rendered clades stable to variation in the analytical parameters. Topologies obtained for the AFLPs, the ITS data alone and the combined analyses, demonstrated the existence of a group formed by I. argentina, I. brasiliensis, I. brevicuspis, I. integerrima, and I. theezans, and that I. dumosa and I. paraguariensis were distantly related to the former. Incongruence with traditional taxonomical treatments was found. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12110/paper_00029122_v92_n2_p352_Gottlieb |
url |
http://hdl.handle.net/20.500.12110/paper_00029122_v92_n2_p352_Gottlieb |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/2.5/ar |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Am. J. Bot. 2005;92(2):352-369 reponame:Biblioteca Digital (UBA-FCEN) instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales instacron:UBA-FCEN |
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instname_str |
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
instacron_str |
UBA-FCEN |
institution |
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repository.name.fl_str_mv |
Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
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ana@bl.fcen.uba.ar |
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