Novel processing in a mammalian nuclear 28S pre-rRNA: Tissue-specific elimination of an 'intron' bearing a hidden break site

Autores
Melen, G.J.; Pesce, C.G.; Rossi, M.S.; Kornblihtt, A.R.
Año de publicación
1999
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Splitting and apparent splicing of ribosomal RNA, both previously unknown in vertebrates, were found in rodents of the genus Ctenomys. Instead of being formed by a single molecule of 4.4 kb, 28S rRNA is split in two molecules of 2.6 and 1.8 kb. A hidden break, mapping within a 106 bp 'intron' located in the D6 divergent region, is expressed in mature ribosomes of liver, lung, heart and spleen, as well as in primary fibroblast cultures. Testis-specific processing eliminates the intron and concomitantly the break site, producing non-split 28S rRNA molecules exclusively in this organ. The intron is flanked by two 9 bp direct repeats, revealing the acquisition by insertion of a novel rRNA processing strategy in the evolution of higher organisms.
Fil:Melen, G.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Pesce, C.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Rossi, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
EMBO J. 1999;18(11):3107-3118
Materia
Mammalian cells
Ribosomal RNA processing
RNA 28s
RNA precursor
animal tissue
article
fibroblast culture
heart
intron
liver
lung
male
nonhuman
priority journal
RNA cleavage
RNA processing
rodent
spleen
testis
tissue specificity
Animals
Base Sequence
Blotting, Northern
Cells, Cultured
Electrophoresis, Polyacrylamide Gel
Evolution, Molecular
Introns
Male
Mice
Models, Genetic
Molecular Sequence Data
Molecular Weight
Nucleic Acid Conformation
Organ Specificity
Rats
Repetitive Sequences, Nucleic Acid
RNA Precursors
RNA Splicing
RNA, Ribosomal, 28S
Rodentia
Testis
Thermodynamics
Animalia
Ctenomys
Mammalia
Rodentia
Vertebrata
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_02614189_v18_n11_p3107_Melen
Acceder
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oai_identifier_str paperaa:paper_02614189_v18_n11_p3107_Melen
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Novel processing in a mammalian nuclear 28S pre-rRNA: Tissue-specific elimination of an 'intron' bearing a hidden break siteMelen, G.J.Pesce, C.G.Rossi, M.S.Kornblihtt, A.R.Mammalian cellsRibosomal RNA processingRNA 28sRNA precursoranimal tissuearticlefibroblast cultureheartintronliverlungmalenonhumanpriority journalRNA cleavageRNA processingrodentspleentestistissue specificityAnimalsBase SequenceBlotting, NorthernCells, CulturedElectrophoresis, Polyacrylamide GelEvolution, MolecularIntronsMaleMiceModels, GeneticMolecular Sequence DataMolecular WeightNucleic Acid ConformationOrgan SpecificityRatsRepetitive Sequences, Nucleic AcidRNA PrecursorsRNA SplicingRNA, Ribosomal, 28SRodentiaTestisThermodynamicsAnimaliaCtenomysMammaliaRodentiaVertebrataSplitting and apparent splicing of ribosomal RNA, both previously unknown in vertebrates, were found in rodents of the genus Ctenomys. Instead of being formed by a single molecule of 4.4 kb, 28S rRNA is split in two molecules of 2.6 and 1.8 kb. A hidden break, mapping within a 106 bp 'intron' located in the D6 divergent region, is expressed in mature ribosomes of liver, lung, heart and spleen, as well as in primary fibroblast cultures. Testis-specific processing eliminates the intron and concomitantly the break site, producing non-split 28S rRNA molecules exclusively in this organ. The intron is flanked by two 9 bp direct repeats, revealing the acquisition by insertion of a novel rRNA processing strategy in the evolution of higher organisms.Fil:Melen, G.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Pesce, C.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Rossi, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.1999info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_02614189_v18_n11_p3107_MelenEMBO J. 1999;18(11):3107-3118reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-10-30T11:21:04Zpaperaa:paper_02614189_v18_n11_p3107_MelenInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-10-30 11:21:06.213Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Novel processing in a mammalian nuclear 28S pre-rRNA: Tissue-specific elimination of an 'intron' bearing a hidden break site
title Novel processing in a mammalian nuclear 28S pre-rRNA: Tissue-specific elimination of an 'intron' bearing a hidden break site
spellingShingle Novel processing in a mammalian nuclear 28S pre-rRNA: Tissue-specific elimination of an 'intron' bearing a hidden break site
Melen, G.J.
Mammalian cells
Ribosomal RNA processing
RNA 28s
RNA precursor
animal tissue
article
fibroblast culture
heart
intron
liver
lung
male
nonhuman
priority journal
RNA cleavage
RNA processing
rodent
spleen
testis
tissue specificity
Animals
Base Sequence
Blotting, Northern
Cells, Cultured
Electrophoresis, Polyacrylamide Gel
Evolution, Molecular
Introns
Male
Mice
Models, Genetic
Molecular Sequence Data
Molecular Weight
Nucleic Acid Conformation
Organ Specificity
Rats
Repetitive Sequences, Nucleic Acid
RNA Precursors
RNA Splicing
RNA, Ribosomal, 28S
Rodentia
Testis
Thermodynamics
Animalia
Ctenomys
Mammalia
Rodentia
Vertebrata
title_short Novel processing in a mammalian nuclear 28S pre-rRNA: Tissue-specific elimination of an 'intron' bearing a hidden break site
title_full Novel processing in a mammalian nuclear 28S pre-rRNA: Tissue-specific elimination of an 'intron' bearing a hidden break site
title_fullStr Novel processing in a mammalian nuclear 28S pre-rRNA: Tissue-specific elimination of an 'intron' bearing a hidden break site
title_full_unstemmed Novel processing in a mammalian nuclear 28S pre-rRNA: Tissue-specific elimination of an 'intron' bearing a hidden break site
title_sort Novel processing in a mammalian nuclear 28S pre-rRNA: Tissue-specific elimination of an 'intron' bearing a hidden break site
dc.creator.none.fl_str_mv Melen, G.J.
Pesce, C.G.
Rossi, M.S.
Kornblihtt, A.R.
author Melen, G.J.
author_facet Melen, G.J.
Pesce, C.G.
Rossi, M.S.
Kornblihtt, A.R.
author_role author
author2 Pesce, C.G.
Rossi, M.S.
Kornblihtt, A.R.
author2_role author
author
author
dc.subject.none.fl_str_mv Mammalian cells
Ribosomal RNA processing
RNA 28s
RNA precursor
animal tissue
article
fibroblast culture
heart
intron
liver
lung
male
nonhuman
priority journal
RNA cleavage
RNA processing
rodent
spleen
testis
tissue specificity
Animals
Base Sequence
Blotting, Northern
Cells, Cultured
Electrophoresis, Polyacrylamide Gel
Evolution, Molecular
Introns
Male
Mice
Models, Genetic
Molecular Sequence Data
Molecular Weight
Nucleic Acid Conformation
Organ Specificity
Rats
Repetitive Sequences, Nucleic Acid
RNA Precursors
RNA Splicing
RNA, Ribosomal, 28S
Rodentia
Testis
Thermodynamics
Animalia
Ctenomys
Mammalia
Rodentia
Vertebrata
topic Mammalian cells
Ribosomal RNA processing
RNA 28s
RNA precursor
animal tissue
article
fibroblast culture
heart
intron
liver
lung
male
nonhuman
priority journal
RNA cleavage
RNA processing
rodent
spleen
testis
tissue specificity
Animals
Base Sequence
Blotting, Northern
Cells, Cultured
Electrophoresis, Polyacrylamide Gel
Evolution, Molecular
Introns
Male
Mice
Models, Genetic
Molecular Sequence Data
Molecular Weight
Nucleic Acid Conformation
Organ Specificity
Rats
Repetitive Sequences, Nucleic Acid
RNA Precursors
RNA Splicing
RNA, Ribosomal, 28S
Rodentia
Testis
Thermodynamics
Animalia
Ctenomys
Mammalia
Rodentia
Vertebrata
dc.description.none.fl_txt_mv Splitting and apparent splicing of ribosomal RNA, both previously unknown in vertebrates, were found in rodents of the genus Ctenomys. Instead of being formed by a single molecule of 4.4 kb, 28S rRNA is split in two molecules of 2.6 and 1.8 kb. A hidden break, mapping within a 106 bp 'intron' located in the D6 divergent region, is expressed in mature ribosomes of liver, lung, heart and spleen, as well as in primary fibroblast cultures. Testis-specific processing eliminates the intron and concomitantly the break site, producing non-split 28S rRNA molecules exclusively in this organ. The intron is flanked by two 9 bp direct repeats, revealing the acquisition by insertion of a novel rRNA processing strategy in the evolution of higher organisms.
Fil:Melen, G.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Pesce, C.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Rossi, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description Splitting and apparent splicing of ribosomal RNA, both previously unknown in vertebrates, were found in rodents of the genus Ctenomys. Instead of being formed by a single molecule of 4.4 kb, 28S rRNA is split in two molecules of 2.6 and 1.8 kb. A hidden break, mapping within a 106 bp 'intron' located in the D6 divergent region, is expressed in mature ribosomes of liver, lung, heart and spleen, as well as in primary fibroblast cultures. Testis-specific processing eliminates the intron and concomitantly the break site, producing non-split 28S rRNA molecules exclusively in this organ. The intron is flanked by two 9 bp direct repeats, revealing the acquisition by insertion of a novel rRNA processing strategy in the evolution of higher organisms.
publishDate 1999
dc.date.none.fl_str_mv 1999
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_02614189_v18_n11_p3107_Melen
url http://hdl.handle.net/20.500.12110/paper_02614189_v18_n11_p3107_Melen
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv EMBO J. 1999;18(11):3107-3118
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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score 13.10058

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