Defective signalling in salivary glands precedes the autoimmune response in the non-obese diabetic mouse model of sialadenitis

• Autores: Rosignoli, F.; Roca, V.; Meiss, R.; Leceta, J.; Gomariz, R.P.; Leirós, C.P.
• Año de publicación: 2005
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Clin. Exp. Immunol. 2005;142(3):411-418
• Materia: Autoimmune response; Nitric oxide signalling; NOD mice; Sialadenitis; Sjögren's syndrome; cyclic AMP; cyclic GMP; neurotransmitter; nitric oxide; nitric oxide synthase; vasoactive intestinal polypeptide; animal experiment; animal model; animal tissue; article; autoimmunity; cell infiltration; cytokine production; enzyme activity; female; immune response; mononuclear cell; mouse; mouse strain; nonhuman; parotid gland; priority journal; salivary gland; salivation; sialoadenitis; signal transduction; Sjoegren syndrome; submandibular gland; Animals; Autoantibodies; Autoimmunity; Cyclic GMP; Cytokines; Diabetes Mellitus, Type 1; Disease Models, Animal; Female; Mice; Mice, Inbred BALB C; Mice, Inbred NOD; Nitric Oxide Synthase; Parotid Gland; Salivary Glands; Sialadenitis; Signal Transduction; Submandibular Gland; Vasoactive Intestinal Peptide
• Institución: Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales

The spontaneous non-obese diabetic (NOD) mouse model of Sjögren's syndrome provides a valuable tool to study the onset and progression of both the autoimmune response and secretory dysfunction. Our purpose was to analyse the temporal decline of salivary secretion in NOD mice in relation to the autoimmune response and alterations in various signalling pathways involved in saliva secretion within each salivary gland. A progressive loss of nitric oxide synthase activity in submandibular and parotid glands started at 12 weeks of age and paralleled the decline in salivary secretion. This defect was associated with a lower response to vasoactive intestinal peptide in salivary flow rate, cAMP and nitric oxide/cGMP production. No signs of mononuclear infiltrates or local cytokine production were detectable in salivary glands in the time period studied (10-16 weeks of age). Our data support a disease model for sialadenitis in NOD mice in which the early stages are characterized by defective neurotransmitter-mediated signalling in major salivary glands that precedes the autoimmune response. © 2005 British Society for Immunology.
Fil:Rosignoli, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Roca, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Leirós, C.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.