Brucella lumazine synthase elicits a mixed Th1-Th2 immune response and reduces infection in mice challenged with Brucella abortus 544 independently of the adjuvant formulation used

• Autores: Velikovsky, C.A.; Goldbaum, F.A.; Cassataro, J.; Estein, S.; Bowden, R.A.; Bruno, L.; Fossati, C.A.; Giambartolomei, G.H.
• Año de publicación: 2003
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Infect. Immun. 2003;71(10):5750-5755
• Materia: aluminum hydroxide; Brucella vaccine; Freund adjuvant; gamma interferon; immunoglobulin G; immunoglobulin G antibody; immunoglobulin G1; immunoglobulin G2; interleukin 10; interleukin 2; interleukin 4; lumazine; lumazine synthase; phosphoryl lipid A; recombinant enzyme; unclassified drug; animal experiment; animal model; article; Brucella abortus; brucellosis; controlled study; cytokine production; drug effect; drug formulation; female; immune response; immunization; immunogenicity; mouse; nonhuman; priority journal; spleen cell; Th1 cell; Th2 cell; Adjuvants, Immunologic; Animals; Antibodies, Bacterial; Brucella; Brucella Vaccine; Brucellosis; Female; Lymphocyte Activation; Mice; Mice, Inbred BALB C; Multienzyme Complexes; Th1 Cells; Th2 Cells; Vaccines, Synthetic
• Institución: Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales

The immunogenicity and protective efficacy of recombinant lumazine synthase from Brucella spp. (rBLS) administered with different adjuvants was evaluated in mice. Mice were immunized with rBLS in the absence or the presence of aluminum hydroxide gel (BLS-A1), monophosphoryl lipid A (BLS-MPA), or incomplete Freund's adjuvant (BLS-IFA). rBLS per se induced a vigorous immunoglobulin G (IgG) response, with high titers of IgG1 as well as IgG2. All the adjuvants increased this response; the BLS-IFA formulation was the most effective at inducing BLS-specific IgG antibodies. In addition, after in vitro stimulation with rBLS, spleen cells from BLS-IFA-, BLS-A1-, or BLS-MPA-immunized mice proliferated and produced interleukin-2 (IL-2), gamma interferon (IFN-γ), IL-10, and IL-4, suggesting the induction of a mixed Th1-Th2 response. Immunization with rBLS protected mice against challenge with B. abortus 544. The levels of protection in the spleen were similar for all adjuvants, but only BLS-A1 and BLS-IFA were effective in the liver. Our results indicate that BLS might be a useful candidate for the development of subunit vaccines against brucellosis, since it elicits antigen-specific cellular responses, with production of IFN-γ and protection, independently of the adjuvant formulation used.