Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stages

Autores
Rodríguez, M.V.; Mendiondo, G.M.; Maskin, L.; Gudesblat, G.E.; Iusem, N.D.; Benech-Arnold, R.L.
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background and AimsPre-harvest sprouting susceptibility in grain sorghum (Sorghum bicolor) is related to low seed dormancy and reduced embryo sensitivity to inhibition of germination by abscisic acid (ABA). Intra-specific variability for pre-harvest sprouting might involve differential regulation of ABA signalling genes.MethodsSorghum genes encoding homologues for ABA signalling components from other species (ABI5, ABI4, VP1, ABI1 and PKABA1) were studied at the transcriptional and protein level (ABI5) during grain imbibition for two sorghum lines with contrasting sprouting phenotypes and in response to hormones.Key ResultsTranscript levels of these genes and protein levels of ABI5 were higher in imbibed immature caryopses of the more dormant line. Dormancy loss was related to lower transcript levels of these genes and lower ABI5 protein levels in both genotypes. Exogenous ABA inhibited germination of isolated embryos but failed to prevent ABI5 rapid decrease supporting a role for the seed coat in regulating ABI5 levels.ConclusionsSeveral genes involved in ABA signalling are regulated differently in imbibed caryopses from two sorghum lines with contrasting pre-harvest sprouting response before - but not after - physiological maturity. A role for ABI5 in the expression of dormancy during grain development is discussed.
Fil:Rodríguez, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Mendiondo, G.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Maskin, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Gudesblat, G.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Iusem, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
Ann. Bot. 2009;104(5):975-985
Materia
ABI5
Abscisic acid
Hormone signalling
Pre-harvest sprouting
Seed dormancy
Sorghum bicolor
abscisic acid
phytohormone
signal transducing adaptor protein
vegetable protein
abscisic acid
developmental stage
embryo
gene expression
harvesting
hormone
imbibition
inhibition
intraspecific variation
protein
seed dormancy
sorghum
article
gene expression regulation
genetics
germination
growth, development and aging
physiology
plant gene
plant seed
polymerase chain reaction
sorghum
Western blotting
Abscisic Acid
Adaptor Proteins, Signal Transducing
Blotting, Western
Gene Expression Regulation, Plant
Genes, Plant
Germination
Plant Growth Regulators
Plant Proteins
Polymerase Chain Reaction
Seeds
Sorghum
Sorghum bicolor
Sorghum bicolor bicolor
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_03057364_v104_n5_p975_Rodriguez
Acceder
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oai_identifier_str paperaa:paper_03057364_v104_n5_p975_Rodriguez
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stagesRodríguez, M.V.Mendiondo, G.M.Maskin, L.Gudesblat, G.E.Iusem, N.D.Benech-Arnold, R.L.ABI5Abscisic acidHormone signallingPre-harvest sproutingSeed dormancySorghum bicolorabscisic acidphytohormonesignal transducing adaptor proteinvegetable proteinabscisic aciddevelopmental stageembryogene expressionharvestinghormoneimbibitioninhibitionintraspecific variationproteinseed dormancysorghumarticlegene expression regulationgeneticsgerminationgrowth, development and agingphysiologyplant geneplant seedpolymerase chain reactionsorghumWestern blottingAbscisic AcidAdaptor Proteins, Signal TransducingBlotting, WesternGene Expression Regulation, PlantGenes, PlantGerminationPlant Growth RegulatorsPlant ProteinsPolymerase Chain ReactionSeedsSorghumSorghum bicolorSorghum bicolor bicolorBackground and AimsPre-harvest sprouting susceptibility in grain sorghum (Sorghum bicolor) is related to low seed dormancy and reduced embryo sensitivity to inhibition of germination by abscisic acid (ABA). Intra-specific variability for pre-harvest sprouting might involve differential regulation of ABA signalling genes.MethodsSorghum genes encoding homologues for ABA signalling components from other species (ABI5, ABI4, VP1, ABI1 and PKABA1) were studied at the transcriptional and protein level (ABI5) during grain imbibition for two sorghum lines with contrasting sprouting phenotypes and in response to hormones.Key ResultsTranscript levels of these genes and protein levels of ABI5 were higher in imbibed immature caryopses of the more dormant line. Dormancy loss was related to lower transcript levels of these genes and lower ABI5 protein levels in both genotypes. Exogenous ABA inhibited germination of isolated embryos but failed to prevent ABI5 rapid decrease supporting a role for the seed coat in regulating ABI5 levels.ConclusionsSeveral genes involved in ABA signalling are regulated differently in imbibed caryopses from two sorghum lines with contrasting pre-harvest sprouting response before - but not after - physiological maturity. A role for ABI5 in the expression of dormancy during grain development is discussed.Fil:Rodríguez, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Mendiondo, G.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Maskin, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Gudesblat, G.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Iusem, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2009info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_03057364_v104_n5_p975_RodriguezAnn. Bot. 2009;104(5):975-985reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-10-16T09:30:09Zpaperaa:paper_03057364_v104_n5_p975_RodriguezInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-10-16 09:30:10.861Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stages
title Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stages
spellingShingle Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stages
Rodríguez, M.V.
ABI5
Abscisic acid
Hormone signalling
Pre-harvest sprouting
Seed dormancy
Sorghum bicolor
abscisic acid
phytohormone
signal transducing adaptor protein
vegetable protein
abscisic acid
developmental stage
embryo
gene expression
harvesting
hormone
imbibition
inhibition
intraspecific variation
protein
seed dormancy
sorghum
article
gene expression regulation
genetics
germination
growth, development and aging
physiology
plant gene
plant seed
polymerase chain reaction
sorghum
Western blotting
Abscisic Acid
Adaptor Proteins, Signal Transducing
Blotting, Western
Gene Expression Regulation, Plant
Genes, Plant
Germination
Plant Growth Regulators
Plant Proteins
Polymerase Chain Reaction
Seeds
Sorghum
Sorghum bicolor
Sorghum bicolor bicolor
title_short Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stages
title_full Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stages
title_fullStr Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stages
title_full_unstemmed Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stages
title_sort Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stages
dc.creator.none.fl_str_mv Rodríguez, M.V.
Mendiondo, G.M.
Maskin, L.
Gudesblat, G.E.
Iusem, N.D.
Benech-Arnold, R.L.
author Rodríguez, M.V.
author_facet Rodríguez, M.V.
Mendiondo, G.M.
Maskin, L.
Gudesblat, G.E.
Iusem, N.D.
Benech-Arnold, R.L.
author_role author
author2 Mendiondo, G.M.
Maskin, L.
Gudesblat, G.E.
Iusem, N.D.
Benech-Arnold, R.L.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ABI5
Abscisic acid
Hormone signalling
Pre-harvest sprouting
Seed dormancy
Sorghum bicolor
abscisic acid
phytohormone
signal transducing adaptor protein
vegetable protein
abscisic acid
developmental stage
embryo
gene expression
harvesting
hormone
imbibition
inhibition
intraspecific variation
protein
seed dormancy
sorghum
article
gene expression regulation
genetics
germination
growth, development and aging
physiology
plant gene
plant seed
polymerase chain reaction
sorghum
Western blotting
Abscisic Acid
Adaptor Proteins, Signal Transducing
Blotting, Western
Gene Expression Regulation, Plant
Genes, Plant
Germination
Plant Growth Regulators
Plant Proteins
Polymerase Chain Reaction
Seeds
Sorghum
Sorghum bicolor
Sorghum bicolor bicolor
topic ABI5
Abscisic acid
Hormone signalling
Pre-harvest sprouting
Seed dormancy
Sorghum bicolor
abscisic acid
phytohormone
signal transducing adaptor protein
vegetable protein
abscisic acid
developmental stage
embryo
gene expression
harvesting
hormone
imbibition
inhibition
intraspecific variation
protein
seed dormancy
sorghum
article
gene expression regulation
genetics
germination
growth, development and aging
physiology
plant gene
plant seed
polymerase chain reaction
sorghum
Western blotting
Abscisic Acid
Adaptor Proteins, Signal Transducing
Blotting, Western
Gene Expression Regulation, Plant
Genes, Plant
Germination
Plant Growth Regulators
Plant Proteins
Polymerase Chain Reaction
Seeds
Sorghum
Sorghum bicolor
Sorghum bicolor bicolor
dc.description.none.fl_txt_mv Background and AimsPre-harvest sprouting susceptibility in grain sorghum (Sorghum bicolor) is related to low seed dormancy and reduced embryo sensitivity to inhibition of germination by abscisic acid (ABA). Intra-specific variability for pre-harvest sprouting might involve differential regulation of ABA signalling genes.MethodsSorghum genes encoding homologues for ABA signalling components from other species (ABI5, ABI4, VP1, ABI1 and PKABA1) were studied at the transcriptional and protein level (ABI5) during grain imbibition for two sorghum lines with contrasting sprouting phenotypes and in response to hormones.Key ResultsTranscript levels of these genes and protein levels of ABI5 were higher in imbibed immature caryopses of the more dormant line. Dormancy loss was related to lower transcript levels of these genes and lower ABI5 protein levels in both genotypes. Exogenous ABA inhibited germination of isolated embryos but failed to prevent ABI5 rapid decrease supporting a role for the seed coat in regulating ABI5 levels.ConclusionsSeveral genes involved in ABA signalling are regulated differently in imbibed caryopses from two sorghum lines with contrasting pre-harvest sprouting response before - but not after - physiological maturity. A role for ABI5 in the expression of dormancy during grain development is discussed.
Fil:Rodríguez, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Mendiondo, G.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Maskin, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Gudesblat, G.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Iusem, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description Background and AimsPre-harvest sprouting susceptibility in grain sorghum (Sorghum bicolor) is related to low seed dormancy and reduced embryo sensitivity to inhibition of germination by abscisic acid (ABA). Intra-specific variability for pre-harvest sprouting might involve differential regulation of ABA signalling genes.MethodsSorghum genes encoding homologues for ABA signalling components from other species (ABI5, ABI4, VP1, ABI1 and PKABA1) were studied at the transcriptional and protein level (ABI5) during grain imbibition for two sorghum lines with contrasting sprouting phenotypes and in response to hormones.Key ResultsTranscript levels of these genes and protein levels of ABI5 were higher in imbibed immature caryopses of the more dormant line. Dormancy loss was related to lower transcript levels of these genes and lower ABI5 protein levels in both genotypes. Exogenous ABA inhibited germination of isolated embryos but failed to prevent ABI5 rapid decrease supporting a role for the seed coat in regulating ABI5 levels.ConclusionsSeveral genes involved in ABA signalling are regulated differently in imbibed caryopses from two sorghum lines with contrasting pre-harvest sprouting response before - but not after - physiological maturity. A role for ABI5 in the expression of dormancy during grain development is discussed.
publishDate 2009
dc.date.none.fl_str_mv 2009
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_03057364_v104_n5_p975_Rodriguez
url http://hdl.handle.net/20.500.12110/paper_03057364_v104_n5_p975_Rodriguez
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Ann. Bot. 2009;104(5):975-985
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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score 12.712165

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