Mammalian smaug is a translational repressor that forms cytoplasmic foci similar to stress granules

• Autores: Baez, M.V.; Boccaccio, G.L.
• Año de publicación: 2005
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: J. Biol. Chem. 2005;280(52):43131-43140
• Materia: Degradation; Genes; Proteins; RNA; Deadenylation; Gene expression; RNA-binding proteins; Cytology; messenger RNA; protein mSmaug 1; protein Smaug; protein Staufen; protein TIA 1; protein TIAR; repressor protein; RNA binding protein; unclassified drug; animal cell; article; cell granule; central nervous system; chromosome 14; cytoplasm; Drosophila; fibroblast; gene expression; genome; human; human cell; molecular recognition; nonhuman; nucleotide sequence; priority journal; protein degradation; protein expression; protein family; reporter gene; RNA sequence; RNA translation; stress; synapse; synaptosome; translation regulation; translation repression; Amino Acid Motifs; Animals; Blotting, Western; Cell Line; Central Nervous System; Cercopithecus aethiops; Chromosomes, Human, Pair 14; COS Cells; Cricetinae; Cytoplasm; Cytoplasmic Granules; DNA, Complementary; Drosophila; Drosophila Proteins; Fibroblasts; Gene Library; Hela Cells; Humans; Luciferases; Mice; Microscopy, Confocal; Microscopy, Fluorescence; Models, Genetic; Molecular Sequence Data; Neurons; Plasmids; Polyribosomes; Protein Binding; Protein Biosynthesis; Repressor Proteins; Reverse Transcriptase Polymerase Chain Reaction; RNA; RNA, Messenger; RNA-Binding Proteins; Synapses; Tissue Distribution; Transfection; Animalia; Mammalia; Murinae
• Institución: Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales

Cytoplasmic events depending on RNA-binding proteins contribute to the fine-tuning of gene expression. Sterile α motif-containing RNA-binding proteins constitute a novel family of post-transcriptional regulators that recognize a specific RNA sequence motif known as Smaug recognition element (SRE). The Drosophila member of this family, dSmaug, triggers the translational repression and deadenylation of maternal mRNAs by independent mechanisms, and the yeast homologue Vts1 stimulates degradation of SRE-containing messengers. Two homologous genes are present in the mammalian genome. Here we showed that hSmaug 1, encoded in human chromosome 14, represses the translation of reporter transcripts carrying SRE motifs. When expressed in fibroblasts, hSmaug 1 forms cytoplasmic granules that contain polyadenylated mRNA and the RNA-binding proteins Staufen, TIAR, TIA-1, and HuR. Smaug 1 foci are distinct from degradation foci. The murine protein mSmaug 1 is expressed in the central nervous system and is abundant in post-synaptic densities, a subcellular region where translation is tightly regulated by synaptic stimulation. Biochemical analysis indicated that mSmaug 1 is present in synaptoneurosomal 20 S particles. These results suggest a role for mammalian Smaug 1 in RNA granule formation and translation regulation in neurons. © 2005 by The American Society for Biochemistry and Molecular Biology, Inc.
Fil:Baez, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Boccaccio, G.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.