Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi.

Autores
Parussini, F.; Duschak, V.G.; Cazzulo, J.J.
Año de publicación
1998
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cysteine proteinase isoforms, immunologically cross-reactive with cruzipain and with a similar apparent molecular mass, have been identified in epimastigotes of Trypanosoma cruzi by extraction and phase partition using the detergent Triton X-114. These isoforms are concentrated in the microsomal fraction obtained after differential centrifugation, which is known to consist essentially of plasma membrane, can be labelled by incubation of live parasites with sulfo-NHS-biotin, and bind to cystatin-sepharose affinity columns. They are present, albeit with a different electrophoretic pattern, in the epimastigote, amastigote and trypomastigote stages of the parasite.
Fil:Parussini, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
Cell. Mol. Biol. (Noisy-le-grand) 1998;44(3):513-519
Materia
cysteine proteinase
isoenzyme
animal
article
cell membrane
enzymology
metabolism
Trypanosoma cruzi
Animals
Cell Membrane
Cysteine Endopeptidases
Isoenzymes
Trypanosoma cruzi
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_01455680_v44_n3_p513_Parussini
Acceder
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oai_identifier_str paperaa:paper_01455680_v44_n3_p513_Parussini
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi.Parussini, F.Duschak, V.G.Cazzulo, J.J.cysteine proteinaseisoenzymeanimalarticlecell membraneenzymologymetabolismTrypanosoma cruziAnimalsCell MembraneCysteine EndopeptidasesIsoenzymesTrypanosoma cruziCysteine proteinase isoforms, immunologically cross-reactive with cruzipain and with a similar apparent molecular mass, have been identified in epimastigotes of Trypanosoma cruzi by extraction and phase partition using the detergent Triton X-114. These isoforms are concentrated in the microsomal fraction obtained after differential centrifugation, which is known to consist essentially of plasma membrane, can be labelled by incubation of live parasites with sulfo-NHS-biotin, and bind to cystatin-sepharose affinity columns. They are present, albeit with a different electrophoretic pattern, in the epimastigote, amastigote and trypomastigote stages of the parasite.Fil:Parussini, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.1998info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_01455680_v44_n3_p513_ParussiniCell. Mol. Biol. (Noisy-le-grand) 1998;44(3):513-519reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:42:51Zpaperaa:paper_01455680_v44_n3_p513_ParussiniInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:42:53.129Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi.
title Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi.
spellingShingle Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi.
Parussini, F.
cysteine proteinase
isoenzyme
animal
article
cell membrane
enzymology
metabolism
Trypanosoma cruzi
Animals
Cell Membrane
Cysteine Endopeptidases
Isoenzymes
Trypanosoma cruzi
title_short Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi.
title_full Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi.
title_fullStr Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi.
title_full_unstemmed Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi.
title_sort Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi.
dc.creator.none.fl_str_mv Parussini, F.
Duschak, V.G.
Cazzulo, J.J.
author Parussini, F.
author_facet Parussini, F.
Duschak, V.G.
Cazzulo, J.J.
author_role author
author2 Duschak, V.G.
Cazzulo, J.J.
author2_role author
author
dc.subject.none.fl_str_mv cysteine proteinase
isoenzyme
animal
article
cell membrane
enzymology
metabolism
Trypanosoma cruzi
Animals
Cell Membrane
Cysteine Endopeptidases
Isoenzymes
Trypanosoma cruzi
topic cysteine proteinase
isoenzyme
animal
article
cell membrane
enzymology
metabolism
Trypanosoma cruzi
Animals
Cell Membrane
Cysteine Endopeptidases
Isoenzymes
Trypanosoma cruzi
dc.description.none.fl_txt_mv Cysteine proteinase isoforms, immunologically cross-reactive with cruzipain and with a similar apparent molecular mass, have been identified in epimastigotes of Trypanosoma cruzi by extraction and phase partition using the detergent Triton X-114. These isoforms are concentrated in the microsomal fraction obtained after differential centrifugation, which is known to consist essentially of plasma membrane, can be labelled by incubation of live parasites with sulfo-NHS-biotin, and bind to cystatin-sepharose affinity columns. They are present, albeit with a different electrophoretic pattern, in the epimastigote, amastigote and trypomastigote stages of the parasite.
Fil:Parussini, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description Cysteine proteinase isoforms, immunologically cross-reactive with cruzipain and with a similar apparent molecular mass, have been identified in epimastigotes of Trypanosoma cruzi by extraction and phase partition using the detergent Triton X-114. These isoforms are concentrated in the microsomal fraction obtained after differential centrifugation, which is known to consist essentially of plasma membrane, can be labelled by incubation of live parasites with sulfo-NHS-biotin, and bind to cystatin-sepharose affinity columns. They are present, albeit with a different electrophoretic pattern, in the epimastigote, amastigote and trypomastigote stages of the parasite.
publishDate 1998
dc.date.none.fl_str_mv 1998
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_01455680_v44_n3_p513_Parussini
url http://hdl.handle.net/20.500.12110/paper_01455680_v44_n3_p513_Parussini
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Cell. Mol. Biol. (Noisy-le-grand) 1998;44(3):513-519
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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score 13.070432

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