Direct Profiling of Phytochemicals in Tulip Tissues and In Vivo Monitoring of the Change of Carbohydrate Content in Tulip Bulbs by Probe Electrospray Ionization Mass Spectrometry

• Autores: Yu, Z.; Chen, L.C.; Suzuki, H.; Ariyada, O.; Erra-Balsells, R.; Nonami, H.; Hiraoka, K.
• Año de publicación: 2009
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: J. Am. Soc. Mass Spectrom. 2009;20(12):2304-2311
• Materia: Bioactive compounds; Carbohydrate content; Direct analysis; Direct profiling; Electrospray ionization mass spectrometry; Electrosprays; Flower buds; In-vivo; Metabolic changes; Phytochemicals; Sample consumption; Sample preparation; Time-of-flight mass spectrometers; Amines; Amino acids; Biological systems; Carbohydrates; Ionization; Mass spectrometers; Mass spectrometry; Organic acids; Particle detectors; Probes; Vegetation; Electrospray ionization; amino acid; anthocyanin; carbohydrate; pelargonidin; plant medicinal product; amino acid analysis; article; carbohydrate analysis; controlled study; drug structure; electrospray mass spectrometry; flower; foliage; in vivo study; nonhuman; petal; plant bulb; plant growth; plant leaf; plant tissue; time of flight mass spectrometry; tulip; Carbohydrates; Plant Roots; Spectrometry, Mass, Electrospray Ionization; Transducers; Tulipa; Tulipa
• Institución: Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales

Probe electrospray ionization (PESI) is a recently developed ESI-based ionization technique which generates electrospray from the tip of a solid needle. In this study, we have applied PESI interfaced with a time of flight mass spectrometer (TOF-MS) for direct profiling of phytochemicals in a section of a tulip bulb in different regions, including basal plate, outer and inner rims of scale, flower bud and foliage leaves. Different parts of tulip petals and leaves have also been investigated. Carbohydrates, amino acids and other phytochemicals were detected. A series of in vivo PESI-MS experiments were carried out on the second outermost scales of four living tulip bulbs to monitoring the change of carbohydrate content during the first week of initial growth. The breakdown of carbohydrates was observed which was in accordance with previous reports achieved by other techniques. This study has indicated that PESI-MS can be used for rapid and direct analysis of phytochemicals in living biological systems with advantages of low sample consumption and little sample preparation. Therefore, PESI-MS can be a new choice for direct analysis/profiling of bioactive compounds or monitoring metabolic changes in living biological systems. © 2009.
Fil:Erra-Balsells, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.