UV-MALDI mass spectrometry analysis of NBD-glycosphingolipids without an external matrix

Autores
Landoni, Malena; Duschak, Vilma G; Erra-Balsells, Rosa; Couto, Alicia S
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Landoni, Malena. CIHIDECAR-Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires; Argentina.
Fil: Duschak, Vilma G. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
Fil: Erra-Balsells, Rosa. Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria; Argentina.
Fil: Couto, Alicia S. Departamento de Quimica Organica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires; Argentina.
Each day, advances in the instrumentation and operating protocols bring new applications and insights into the molecular processes of ultra violet-matrix assisted laser desorption/ionization-mass spectrometry (UV-MALDI MS), increasing its potential use. We report here an approach in which mass spectrometry analysis of sphingolipids has been performed using a fluorescent tag (nitrobenz-2-oxa-1, 3-diazole, NBD) covalently linked to the sphingoid base as matrix. Thus, different labeled-sphingolipids were analyzed: ceramide, dihydroceramide, acetylceramide, glucosylceramide, galactosylceramide, galactosyldihydroceramide. In addition an extract of glycosphingolipids obtained from epimastigote forms of Trypanosoma cruzi metabolically labeled with NBD-ceramide was analyzed. The goal of this work is to show that no matrix needs to be added for the mass spectrometry analysis as the same tag used to label the lipids may generate efficiently analyte ions to obtain high quality signals.
Fuente
Journal of the American Society for Mass Spectrometry 2008; 19(7):923-6.
Materia
Análisis Espectral
Glicoesfingolípidos
Nivel de accesibilidad
acceso abierto
Condiciones de uso
none
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:123456789/2082

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network_acronym_str SGCANLIS
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network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling UV-MALDI mass spectrometry analysis of NBD-glycosphingolipids without an external matrixLandoni, MalenaDuschak, Vilma GErra-Balsells, RosaCouto, Alicia SAnálisis EspectralGlicoesfingolípidosFil: Landoni, Malena. CIHIDECAR-Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires; Argentina.Fil: Duschak, Vilma G. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.Fil: Erra-Balsells, Rosa. Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria; Argentina.Fil: Couto, Alicia S. Departamento de Quimica Organica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires; Argentina.Each day, advances in the instrumentation and operating protocols bring new applications and insights into the molecular processes of ultra violet-matrix assisted laser desorption/ionization-mass spectrometry (UV-MALDI MS), increasing its potential use. We report here an approach in which mass spectrometry analysis of sphingolipids has been performed using a fluorescent tag (nitrobenz-2-oxa-1, 3-diazole, NBD) covalently linked to the sphingoid base as matrix. Thus, different labeled-sphingolipids were analyzed: ceramide, dihydroceramide, acetylceramide, glucosylceramide, galactosylceramide, galactosyldihydroceramide. In addition an extract of glycosphingolipids obtained from epimastigote forms of Trypanosoma cruzi metabolically labeled with NBD-ceramide was analyzed. The goal of this work is to show that no matrix needs to be added for the mass spectrometry analysis as the same tag used to label the lipids may generate efficiently analyte ions to obtain high quality signals.American Chemical Society2008-07info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1044-0305http://sgc.anlis.gob.ar/handle/123456789/208210.1016/j.jasms.2008.04.017Journal of the American Society for Mass Spectrometry 2008; 19(7):923-6.reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISJournal of the American Society for Mass Spectrometrynoneinfo:eu-repo/semantics/openAccesseng2025-09-29T14:30:37Zoai:sgc.anlis.gob.ar:123456789/2082Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-29 14:30:38.034Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv UV-MALDI mass spectrometry analysis of NBD-glycosphingolipids without an external matrix
title UV-MALDI mass spectrometry analysis of NBD-glycosphingolipids without an external matrix
spellingShingle UV-MALDI mass spectrometry analysis of NBD-glycosphingolipids without an external matrix
Landoni, Malena
Análisis Espectral
Glicoesfingolípidos
title_short UV-MALDI mass spectrometry analysis of NBD-glycosphingolipids without an external matrix
title_full UV-MALDI mass spectrometry analysis of NBD-glycosphingolipids without an external matrix
title_fullStr UV-MALDI mass spectrometry analysis of NBD-glycosphingolipids without an external matrix
title_full_unstemmed UV-MALDI mass spectrometry analysis of NBD-glycosphingolipids without an external matrix
title_sort UV-MALDI mass spectrometry analysis of NBD-glycosphingolipids without an external matrix
dc.creator.none.fl_str_mv Landoni, Malena
Duschak, Vilma G
Erra-Balsells, Rosa
Couto, Alicia S
author Landoni, Malena
author_facet Landoni, Malena
Duschak, Vilma G
Erra-Balsells, Rosa
Couto, Alicia S
author_role author
author2 Duschak, Vilma G
Erra-Balsells, Rosa
Couto, Alicia S
author2_role author
author
author
dc.subject.none.fl_str_mv Análisis Espectral
Glicoesfingolípidos
topic Análisis Espectral
Glicoesfingolípidos
dc.description.none.fl_txt_mv Fil: Landoni, Malena. CIHIDECAR-Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires; Argentina.
Fil: Duschak, Vilma G. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
Fil: Erra-Balsells, Rosa. Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria; Argentina.
Fil: Couto, Alicia S. Departamento de Quimica Organica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires; Argentina.
Each day, advances in the instrumentation and operating protocols bring new applications and insights into the molecular processes of ultra violet-matrix assisted laser desorption/ionization-mass spectrometry (UV-MALDI MS), increasing its potential use. We report here an approach in which mass spectrometry analysis of sphingolipids has been performed using a fluorescent tag (nitrobenz-2-oxa-1, 3-diazole, NBD) covalently linked to the sphingoid base as matrix. Thus, different labeled-sphingolipids were analyzed: ceramide, dihydroceramide, acetylceramide, glucosylceramide, galactosylceramide, galactosyldihydroceramide. In addition an extract of glycosphingolipids obtained from epimastigote forms of Trypanosoma cruzi metabolically labeled with NBD-ceramide was analyzed. The goal of this work is to show that no matrix needs to be added for the mass spectrometry analysis as the same tag used to label the lipids may generate efficiently analyte ions to obtain high quality signals.
description Fil: Landoni, Malena. CIHIDECAR-Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires; Argentina.
publishDate 2008
dc.date.none.fl_str_mv 2008-07
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 1044-0305
http://sgc.anlis.gob.ar/handle/123456789/2082
10.1016/j.jasms.2008.04.017
identifier_str_mv 1044-0305
10.1016/j.jasms.2008.04.017
url http://sgc.anlis.gob.ar/handle/123456789/2082
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of the American Society for Mass Spectrometry
dc.rights.none.fl_str_mv none
info:eu-repo/semantics/openAccess
rights_invalid_str_mv none
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv Journal of the American Society for Mass Spectrometry 2008; 19(7):923-6.
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
collection Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
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