UV-MALDI mass spectrometry analysis of NBD-glycosphingolipids without an external matrix
- Autores
- Landoni, Malena; Duschak, Vilma G; Erra-Balsells, Rosa; Couto, Alicia S
- Año de publicación
- 2008
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fil: Landoni, Malena. CIHIDECAR-Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires; Argentina.
Fil: Duschak, Vilma G. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
Fil: Erra-Balsells, Rosa. Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria; Argentina.
Fil: Couto, Alicia S. Departamento de Quimica Organica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires; Argentina.
Each day, advances in the instrumentation and operating protocols bring new applications and insights into the molecular processes of ultra violet-matrix assisted laser desorption/ionization-mass spectrometry (UV-MALDI MS), increasing its potential use. We report here an approach in which mass spectrometry analysis of sphingolipids has been performed using a fluorescent tag (nitrobenz-2-oxa-1, 3-diazole, NBD) covalently linked to the sphingoid base as matrix. Thus, different labeled-sphingolipids were analyzed: ceramide, dihydroceramide, acetylceramide, glucosylceramide, galactosylceramide, galactosyldihydroceramide. In addition an extract of glycosphingolipids obtained from epimastigote forms of Trypanosoma cruzi metabolically labeled with NBD-ceramide was analyzed. The goal of this work is to show that no matrix needs to be added for the mass spectrometry analysis as the same tag used to label the lipids may generate efficiently analyte ions to obtain high quality signals. - Fuente
- Journal of the American Society for Mass Spectrometry 2008; 19(7):923-6.
- Materia
-
Análisis Espectral
Glicoesfingolípidos - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- none
- Repositorio
- Institución
- Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
- OAI Identificador
- oai:sgc.anlis.gob.ar:123456789/2082
Ver los metadatos del registro completo
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UV-MALDI mass spectrometry analysis of NBD-glycosphingolipids without an external matrixLandoni, MalenaDuschak, Vilma GErra-Balsells, RosaCouto, Alicia SAnálisis EspectralGlicoesfingolípidosFil: Landoni, Malena. CIHIDECAR-Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires; Argentina.Fil: Duschak, Vilma G. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.Fil: Erra-Balsells, Rosa. Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria; Argentina.Fil: Couto, Alicia S. Departamento de Quimica Organica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires; Argentina.Each day, advances in the instrumentation and operating protocols bring new applications and insights into the molecular processes of ultra violet-matrix assisted laser desorption/ionization-mass spectrometry (UV-MALDI MS), increasing its potential use. We report here an approach in which mass spectrometry analysis of sphingolipids has been performed using a fluorescent tag (nitrobenz-2-oxa-1, 3-diazole, NBD) covalently linked to the sphingoid base as matrix. Thus, different labeled-sphingolipids were analyzed: ceramide, dihydroceramide, acetylceramide, glucosylceramide, galactosylceramide, galactosyldihydroceramide. In addition an extract of glycosphingolipids obtained from epimastigote forms of Trypanosoma cruzi metabolically labeled with NBD-ceramide was analyzed. The goal of this work is to show that no matrix needs to be added for the mass spectrometry analysis as the same tag used to label the lipids may generate efficiently analyte ions to obtain high quality signals.American Chemical Society2008-07info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1044-0305http://sgc.anlis.gob.ar/handle/123456789/208210.1016/j.jasms.2008.04.017Journal of the American Society for Mass Spectrometry 2008; 19(7):923-6.reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISJournal of the American Society for Mass Spectrometrynoneinfo:eu-repo/semantics/openAccesseng2025-09-29T14:30:37Zoai:sgc.anlis.gob.ar:123456789/2082Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-29 14:30:38.034Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false |
dc.title.none.fl_str_mv |
UV-MALDI mass spectrometry analysis of NBD-glycosphingolipids without an external matrix |
title |
UV-MALDI mass spectrometry analysis of NBD-glycosphingolipids without an external matrix |
spellingShingle |
UV-MALDI mass spectrometry analysis of NBD-glycosphingolipids without an external matrix Landoni, Malena Análisis Espectral Glicoesfingolípidos |
title_short |
UV-MALDI mass spectrometry analysis of NBD-glycosphingolipids without an external matrix |
title_full |
UV-MALDI mass spectrometry analysis of NBD-glycosphingolipids without an external matrix |
title_fullStr |
UV-MALDI mass spectrometry analysis of NBD-glycosphingolipids without an external matrix |
title_full_unstemmed |
UV-MALDI mass spectrometry analysis of NBD-glycosphingolipids without an external matrix |
title_sort |
UV-MALDI mass spectrometry analysis of NBD-glycosphingolipids without an external matrix |
dc.creator.none.fl_str_mv |
Landoni, Malena Duschak, Vilma G Erra-Balsells, Rosa Couto, Alicia S |
author |
Landoni, Malena |
author_facet |
Landoni, Malena Duschak, Vilma G Erra-Balsells, Rosa Couto, Alicia S |
author_role |
author |
author2 |
Duschak, Vilma G Erra-Balsells, Rosa Couto, Alicia S |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Análisis Espectral Glicoesfingolípidos |
topic |
Análisis Espectral Glicoesfingolípidos |
dc.description.none.fl_txt_mv |
Fil: Landoni, Malena. CIHIDECAR-Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires; Argentina. Fil: Duschak, Vilma G. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina. Fil: Erra-Balsells, Rosa. Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria; Argentina. Fil: Couto, Alicia S. Departamento de Quimica Organica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires; Argentina. Each day, advances in the instrumentation and operating protocols bring new applications and insights into the molecular processes of ultra violet-matrix assisted laser desorption/ionization-mass spectrometry (UV-MALDI MS), increasing its potential use. We report here an approach in which mass spectrometry analysis of sphingolipids has been performed using a fluorescent tag (nitrobenz-2-oxa-1, 3-diazole, NBD) covalently linked to the sphingoid base as matrix. Thus, different labeled-sphingolipids were analyzed: ceramide, dihydroceramide, acetylceramide, glucosylceramide, galactosylceramide, galactosyldihydroceramide. In addition an extract of glycosphingolipids obtained from epimastigote forms of Trypanosoma cruzi metabolically labeled with NBD-ceramide was analyzed. The goal of this work is to show that no matrix needs to be added for the mass spectrometry analysis as the same tag used to label the lipids may generate efficiently analyte ions to obtain high quality signals. |
description |
Fil: Landoni, Malena. CIHIDECAR-Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires; Argentina. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-07 |
dc.type.none.fl_str_mv |
info:ar-repo/semantics/articulo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
1044-0305 http://sgc.anlis.gob.ar/handle/123456789/2082 10.1016/j.jasms.2008.04.017 |
identifier_str_mv |
1044-0305 10.1016/j.jasms.2008.04.017 |
url |
http://sgc.anlis.gob.ar/handle/123456789/2082 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of the American Society for Mass Spectrometry |
dc.rights.none.fl_str_mv |
none info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
none |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
American Chemical Society |
publisher.none.fl_str_mv |
American Chemical Society |
dc.source.none.fl_str_mv |
Journal of the American Society for Mass Spectrometry 2008; 19(7):923-6. reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" instacron:ANLIS |
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Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
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Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
instname_str |
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" |
instacron_str |
ANLIS |
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ANLIS |
repository.name.fl_str_mv |
Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" |
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biblioteca@anlis.gov.ar |
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12.559606 |