Population structure and diversity of the pathogenic fungus Aspergillus fumigatus isolated from different sources and geographic origins

• Autores: Duarte-Escalante, Esperanza; Zúñiga, Gerardo; Nava Ramírez, Oscar; Córdoba, Susana; Refojo, Nicolás; Arenas, Roberto; Delhaes, Laurence; Reyes-Montes, María del Rocío
• Año de publicación: 2009
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Memórias do Instituto Oswaldo Cruz 2009;104(3):427–433
• Materia: Aspergillus fumigatus; Análisis del Polimorfismo de Longitud de Fragmentos Amplificados; Variación Genética
• Institución: Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"

Fil: Duarte-Escalante, Esperanza. Universidad Nacional Autónoma de México. Laboratorio de Micología Molecular; México.
Fil: Zúñiga, Gerardo. Instituto Politécnico Nacional. Departamento de Zoología; México.
Fil: Nava Ramírez, Oscar. Universidad Nacional Autónoma de México. Laboratorio de Micología Molecular; México.
Fil: Córdoba, Susana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Departamento Micología; Argentina.
Fil: Refojo, Nicolás. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Departamento Micología; Argentina.
Fil: Arenas, Roberto. Hospital General Dr. Manuel Gea González; México.
Fil: Delhaes, Laurence. Pasteur Institute of Lille; Francia.
Fil: Reyes-Montes, María del Rocío. Universidad Nacional Autónoma de México. Laboratorio de Micología Molecular; México.
Fifty-five clinical and environmental Aspergillus fumigatusisolates from Mexico, Argentina, France and Peru were analyzed to determine their genetic variability, reproductive system and level of differentiation using amplified fragment length polymorphismmarkers. The level of genetic variability was assessed by measuring the percentage of polymorphic loci, number of effective alleles, expected heterozygocity and by performing an association index test (I A ). The degree of genetic differentiation and variation was determined using analysis of molecular variance at three levels. Using the paired genetic distances, a dendrogram was built to detect the genetic relationship among alleles. Finally, a network of haplotypes was constructed to determine the geographic relationship among them. The results indicate that the clinical isolates have greater genetic variability than the environmental isolates. The I A of the clinical and environmental isolates suggests a recombining population structure. The genetic differentiation among isolates and the dendrogram suggest that the groups of isolates are different. The network of haplotypes demonstrates that the majority of the isolates are grouped according to geographic origin.