Molecular identification of mumps virus genotypes from clinical samples: Standardized method of analysis

Autores
Palacios, Gustavo; Jabado, Omar J.; Cisterna, Daniel; de Ory, F.; Renwick, N.; Echevarria, J. E.; Castellanos, A.; Mosquera, M.; Freire, María Cecilia; Campos, Rodolfo; Lipkin, W. Ian
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Palacios, Gustavo. Columbia University. Jerome L. and Dawn Greene Infectious Disease Laboratory; Estados Unidos.
Fil: Jabado, O. Columbia University. Jerome L. and Dawn Greene Infectious Disease Laboratory; Estados Unidos.
Fil: Cisterna, Daniel. Instituto de Salud Carlos III. Services of Diagnostic Microbiology and Virology; España.
Fil: de Ory, F. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología. División Neurovirosis; Argentina.
Fil: Renwick, N. Columbia University. Jerome L. and Dawn Greene Infectious Disease Laboratory; Estados Unidos.
Fil: Echevarria, J. E. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología. División Neurovirosis; Argentina.
Fil: Castellanos, A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología. División Neurovirosis; Argentina.
Fil: Mosquera, M. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología. División Neurovirosis; Argentina.
Fil: Freire, María Cecilia. tituto de Salud Carlos III. Services of Diagnostic Microbiology and Virology; España.
Fil: Campos, Rodolfo. Universidad de Buenos Aires. Cátedra de Virología; Argentina.
Fil: Lipkin, W. Ian. Columbia University. Jerome L. and Dawn Greene Infectious Disease Laboratory; Estados Unidos.
A sensitive nested reverse transcription-PCR assay, targeting a short fragment of the gene encoding the small hydrophobic protein (SH gene), was developed to allow rapid characterization of mumps virus in clinical samples. The sensitivity and specificity of the assay were established using representative genotypes A, B, C, D, E, and F. Mumps virus RNA was characterized directly from cerebrospinal fluid (CSF) samples and in extracts of mumps virus isolates from patients with various clinical syndromes. Direct sequencing of products and subsequent phylogenetic analysis enabled genetic classification. A simple web-based system of sequence analysis was established. The study also allowed characterization of mumps virus strains from Argentina as part of a new subgenotype. This PCR assay for characterization of mumps infections coupled to a web-based analytical program provides a rapid method for identification of known and novel strains.
Fuente
Journal of Clinical Microbiology 2005;43(4):1869–1878
Materia
Virus de la Parotiditis
Sensibilidad y Especificidad
Reacción en Cadena de la Polimerasa
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:123456789/291
Acceder
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network_acronym_str SGCANLIS
repository_id_str a
network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling Molecular identification of mumps virus genotypes from clinical samples: Standardized method of analysisPalacios, GustavoJabado, Omar J.Cisterna, Danielde Ory, F.Renwick, N.Echevarria, J. E.Castellanos, A.Mosquera, M.Freire, María CeciliaCampos, RodolfoLipkin, W. IanVirus de la ParotiditisSensibilidad y EspecificidadReacción en Cadena de la PolimerasaFil: Palacios, Gustavo. Columbia University. Jerome L. and Dawn Greene Infectious Disease Laboratory; Estados Unidos.Fil: Jabado, O. Columbia University. Jerome L. and Dawn Greene Infectious Disease Laboratory; Estados Unidos.Fil: Cisterna, Daniel. Instituto de Salud Carlos III. Services of Diagnostic Microbiology and Virology; España.Fil: de Ory, F. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología. División Neurovirosis; Argentina.Fil: Renwick, N. Columbia University. Jerome L. and Dawn Greene Infectious Disease Laboratory; Estados Unidos.Fil: Echevarria, J. E. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología. División Neurovirosis; Argentina.Fil: Castellanos, A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología. División Neurovirosis; Argentina.Fil: Mosquera, M. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología. División Neurovirosis; Argentina.Fil: Freire, María Cecilia. tituto de Salud Carlos III. Services of Diagnostic Microbiology and Virology; España.Fil: Campos, Rodolfo. Universidad de Buenos Aires. Cátedra de Virología; Argentina.Fil: Lipkin, W. Ian. Columbia University. Jerome L. and Dawn Greene Infectious Disease Laboratory; Estados Unidos.A sensitive nested reverse transcription-PCR assay, targeting a short fragment of the gene encoding the small hydrophobic protein (SH gene), was developed to allow rapid characterization of mumps virus in clinical samples. The sensitivity and specificity of the assay were established using representative genotypes A, B, C, D, E, and F. Mumps virus RNA was characterized directly from cerebrospinal fluid (CSF) samples and in extracts of mumps virus isolates from patients with various clinical syndromes. Direct sequencing of products and subsequent phylogenetic analysis enabled genetic classification. A simple web-based system of sequence analysis was established. The study also allowed characterization of mumps virus strains from Argentina as part of a new subgenotype. This PCR assay for characterization of mumps infections coupled to a web-based analytical program provides a rapid method for identification of known and novel strains.2005-04info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1098-660Xhttp://sgc.anlis.gob.ar/handle/123456789/291http://jcm.asm.org/content/43/4/1869.full.pdf+htmlJournal of Clinical Microbiology 2005;43(4):1869–1878reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISJournal of clinical microbiologyenginfo:eu-repo/semantics/openAccess2025-10-23T11:19:36Zoai:sgc.anlis.gob.ar:123456789/291Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-10-23 11:19:36.248Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv Molecular identification of mumps virus genotypes from clinical samples: Standardized method of analysis
title Molecular identification of mumps virus genotypes from clinical samples: Standardized method of analysis
spellingShingle Molecular identification of mumps virus genotypes from clinical samples: Standardized method of analysis
Palacios, Gustavo
Virus de la Parotiditis
Sensibilidad y Especificidad
Reacción en Cadena de la Polimerasa
title_short Molecular identification of mumps virus genotypes from clinical samples: Standardized method of analysis
title_full Molecular identification of mumps virus genotypes from clinical samples: Standardized method of analysis
title_fullStr Molecular identification of mumps virus genotypes from clinical samples: Standardized method of analysis
title_full_unstemmed Molecular identification of mumps virus genotypes from clinical samples: Standardized method of analysis
title_sort Molecular identification of mumps virus genotypes from clinical samples: Standardized method of analysis
dc.creator.none.fl_str_mv Palacios, Gustavo
Jabado, Omar J.
Cisterna, Daniel
de Ory, F.
Renwick, N.
Echevarria, J. E.
Castellanos, A.
Mosquera, M.
Freire, María Cecilia
Campos, Rodolfo
Lipkin, W. Ian
author Palacios, Gustavo
author_facet Palacios, Gustavo
Jabado, Omar J.
Cisterna, Daniel
de Ory, F.
Renwick, N.
Echevarria, J. E.
Castellanos, A.
Mosquera, M.
Freire, María Cecilia
Campos, Rodolfo
Lipkin, W. Ian
author_role author
author2 Jabado, Omar J.
Cisterna, Daniel
de Ory, F.
Renwick, N.
Echevarria, J. E.
Castellanos, A.
Mosquera, M.
Freire, María Cecilia
Campos, Rodolfo
Lipkin, W. Ian
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Virus de la Parotiditis
Sensibilidad y Especificidad
Reacción en Cadena de la Polimerasa
topic Virus de la Parotiditis
Sensibilidad y Especificidad
Reacción en Cadena de la Polimerasa
dc.description.none.fl_txt_mv Fil: Palacios, Gustavo. Columbia University. Jerome L. and Dawn Greene Infectious Disease Laboratory; Estados Unidos.
Fil: Jabado, O. Columbia University. Jerome L. and Dawn Greene Infectious Disease Laboratory; Estados Unidos.
Fil: Cisterna, Daniel. Instituto de Salud Carlos III. Services of Diagnostic Microbiology and Virology; España.
Fil: de Ory, F. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología. División Neurovirosis; Argentina.
Fil: Renwick, N. Columbia University. Jerome L. and Dawn Greene Infectious Disease Laboratory; Estados Unidos.
Fil: Echevarria, J. E. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología. División Neurovirosis; Argentina.
Fil: Castellanos, A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología. División Neurovirosis; Argentina.
Fil: Mosquera, M. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología. División Neurovirosis; Argentina.
Fil: Freire, María Cecilia. tituto de Salud Carlos III. Services of Diagnostic Microbiology and Virology; España.
Fil: Campos, Rodolfo. Universidad de Buenos Aires. Cátedra de Virología; Argentina.
Fil: Lipkin, W. Ian. Columbia University. Jerome L. and Dawn Greene Infectious Disease Laboratory; Estados Unidos.
A sensitive nested reverse transcription-PCR assay, targeting a short fragment of the gene encoding the small hydrophobic protein (SH gene), was developed to allow rapid characterization of mumps virus in clinical samples. The sensitivity and specificity of the assay were established using representative genotypes A, B, C, D, E, and F. Mumps virus RNA was characterized directly from cerebrospinal fluid (CSF) samples and in extracts of mumps virus isolates from patients with various clinical syndromes. Direct sequencing of products and subsequent phylogenetic analysis enabled genetic classification. A simple web-based system of sequence analysis was established. The study also allowed characterization of mumps virus strains from Argentina as part of a new subgenotype. This PCR assay for characterization of mumps infections coupled to a web-based analytical program provides a rapid method for identification of known and novel strains.
description Fil: Palacios, Gustavo. Columbia University. Jerome L. and Dawn Greene Infectious Disease Laboratory; Estados Unidos.
publishDate 2005
dc.date.none.fl_str_mv 2005-04
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 1098-660X
http://sgc.anlis.gob.ar/handle/123456789/291
http://jcm.asm.org/content/43/4/1869.full.pdf+html
identifier_str_mv 1098-660X
url http://sgc.anlis.gob.ar/handle/123456789/291
http://jcm.asm.org/content/43/4/1869.full.pdf+html
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of clinical microbiology
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Journal of Clinical Microbiology 2005;43(4):1869–1878
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
collection Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
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