Emergence and spread of Neisseria gonorrhoeae isolates with decreased susceptibility to extended-spectrum Cephalosporins in Argentina, 2009 to 2013
- Autores
- Gianecini, Ricardo A; Romero, María Mercedes; Oviedo, Claudia; Vacchino, Martin; Galarza, Patricia G.
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fil: Gianecini, Ricardo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Romero, Maria de Las Mercedes. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Oviedo, Claudia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Vacchino, Martin. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Galarza, Patricia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Background: The emergence of Neisseria gonorrhoeae strains with decreased susceptibility to cephalosporins represents a major concern globally. The aim of this study was to examine the phenotypic and molecular characteristics of N. gonorrhoeae isolates with decreased susceptibility to ceftriaxone and cefixime in Argentina. Methods: A total of 1987 isolates were collected during 2009 and 2013. The susceptibility to penicillin G, tetracycline, ciprofloxacin, cefixime, ceftriaxone, and azithromycin was determined using the agar dilution method. The major extended-spectrum cephalosporin resistance determinants (penA, mtrR, and porB1b) were sequenced in 42 N. gonorrhoeae isolates that showed decreased susceptibility to ceftriaxone (minimum inhibitory concentration [MIC], 0.06-0.125 mg/L) and cefixime (MIC, 0.125-0.25 mg/L). Genotyping by N. gonorrhoeae multiantigen sequence typing (NG-MAST) was performed. Results: Between 2009 and 2013, there was a shift in the modal MICs for ceftriaxone. Among the 42 isolates exhibiting decreased susceptibility to ceftriaxone and cefixime, 95.2% were resistant to penicillin G, 95.2% to tetracycline, 97.6% to ciprofloxacin, and 33.3% to azithromycin. Thirty-five (83.3%) of the 42 isolates had a mosaic penA allele XXXIV, which has been previously associated with resistance to ceftriaxone and cefixime as well as treatment failures. The isolates that contained the mosaic penicillin-binding protein 2 (PBP2) XXXIV were associated with NG-MAST ST1407 or closely related genotypes. Conclusions: In Argentina, N. gonorrhoeae isolates with decreased susceptibility to cefixime and ceftriaxone have now emerged, mostly due to the introduction of the internationally spread multidrug-resistant NG-MAST ST1407. - Fuente
- Sexually Transmitted Diseases 2017; 44(6):351-355
- Materia
-
Antibacterianos
Argentina
Cefixima
Ceftriaxona
Resistencia a las Cefalosporinas
Gonorrea
Humanos
Pruebas de Sensibilidad Microbiana
Tipificación Molecular
Neisseria gonorrhoeae
Filogenia
Vigilancia de Guardia
Análisis de Secuencia de ADN - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- Repositorio
- Institución
- Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
- OAI Identificador
- oai:sgc.anlis.gob.ar:Publications/123456789/1813
Ver los metadatos del registro completo
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Emergence and spread of Neisseria gonorrhoeae isolates with decreased susceptibility to extended-spectrum Cephalosporins in Argentina, 2009 to 2013Gianecini, Ricardo ARomero, María MercedesOviedo, ClaudiaVacchino, MartinGalarza, Patricia G.AntibacterianosArgentinaCefiximaCeftriaxonaResistencia a las CefalosporinasGonorreaHumanosPruebas de Sensibilidad MicrobianaTipificación MolecularNeisseria gonorrhoeaeFilogeniaVigilancia de GuardiaAnálisis de Secuencia de ADNFil: Gianecini, Ricardo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Romero, Maria de Las Mercedes. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Oviedo, Claudia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Vacchino, Martin. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Fil: Galarza, Patricia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.Background: The emergence of Neisseria gonorrhoeae strains with decreased susceptibility to cephalosporins represents a major concern globally. The aim of this study was to examine the phenotypic and molecular characteristics of N. gonorrhoeae isolates with decreased susceptibility to ceftriaxone and cefixime in Argentina. Methods: A total of 1987 isolates were collected during 2009 and 2013. The susceptibility to penicillin G, tetracycline, ciprofloxacin, cefixime, ceftriaxone, and azithromycin was determined using the agar dilution method. The major extended-spectrum cephalosporin resistance determinants (penA, mtrR, and porB1b) were sequenced in 42 N. gonorrhoeae isolates that showed decreased susceptibility to ceftriaxone (minimum inhibitory concentration [MIC], 0.06-0.125 mg/L) and cefixime (MIC, 0.125-0.25 mg/L). Genotyping by N. gonorrhoeae multiantigen sequence typing (NG-MAST) was performed. Results: Between 2009 and 2013, there was a shift in the modal MICs for ceftriaxone. Among the 42 isolates exhibiting decreased susceptibility to ceftriaxone and cefixime, 95.2% were resistant to penicillin G, 95.2% to tetracycline, 97.6% to ciprofloxacin, and 33.3% to azithromycin. Thirty-five (83.3%) of the 42 isolates had a mosaic penA allele XXXIV, which has been previously associated with resistance to ceftriaxone and cefixime as well as treatment failures. The isolates that contained the mosaic penicillin-binding protein 2 (PBP2) XXXIV were associated with NG-MAST ST1407 or closely related genotypes. Conclusions: In Argentina, N. gonorrhoeae isolates with decreased susceptibility to cefixime and ceftriaxone have now emerged, mostly due to the introduction of the internationally spread multidrug-resistant NG-MAST ST1407.Lippincott, Williams & Wilkins2017-06info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://sgc.anlis.gob.ar/handle/123456789/181310.1097/OLQ.0000000000000603Sexually Transmitted Diseases 2017; 44(6):351-355reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLIS#PLACEHOLDER_PARENT_METADATA_VALUE#datasetsSexually transmitted diseasesenginfo:eu-repo/semantics/openAccess2025-09-29T14:30:28Zoai:sgc.anlis.gob.ar:Publications/123456789/1813Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-29 14:30:28.491Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false |
dc.title.none.fl_str_mv |
Emergence and spread of Neisseria gonorrhoeae isolates with decreased susceptibility to extended-spectrum Cephalosporins in Argentina, 2009 to 2013 |
title |
Emergence and spread of Neisseria gonorrhoeae isolates with decreased susceptibility to extended-spectrum Cephalosporins in Argentina, 2009 to 2013 |
spellingShingle |
Emergence and spread of Neisseria gonorrhoeae isolates with decreased susceptibility to extended-spectrum Cephalosporins in Argentina, 2009 to 2013 Gianecini, Ricardo A Antibacterianos Argentina Cefixima Ceftriaxona Resistencia a las Cefalosporinas Gonorrea Humanos Pruebas de Sensibilidad Microbiana Tipificación Molecular Neisseria gonorrhoeae Filogenia Vigilancia de Guardia Análisis de Secuencia de ADN |
title_short |
Emergence and spread of Neisseria gonorrhoeae isolates with decreased susceptibility to extended-spectrum Cephalosporins in Argentina, 2009 to 2013 |
title_full |
Emergence and spread of Neisseria gonorrhoeae isolates with decreased susceptibility to extended-spectrum Cephalosporins in Argentina, 2009 to 2013 |
title_fullStr |
Emergence and spread of Neisseria gonorrhoeae isolates with decreased susceptibility to extended-spectrum Cephalosporins in Argentina, 2009 to 2013 |
title_full_unstemmed |
Emergence and spread of Neisseria gonorrhoeae isolates with decreased susceptibility to extended-spectrum Cephalosporins in Argentina, 2009 to 2013 |
title_sort |
Emergence and spread of Neisseria gonorrhoeae isolates with decreased susceptibility to extended-spectrum Cephalosporins in Argentina, 2009 to 2013 |
dc.creator.none.fl_str_mv |
Gianecini, Ricardo A Romero, María Mercedes Oviedo, Claudia Vacchino, Martin Galarza, Patricia G. |
author |
Gianecini, Ricardo A |
author_facet |
Gianecini, Ricardo A Romero, María Mercedes Oviedo, Claudia Vacchino, Martin Galarza, Patricia G. |
author_role |
author |
author2 |
Romero, María Mercedes Oviedo, Claudia Vacchino, Martin Galarza, Patricia G. |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Antibacterianos Argentina Cefixima Ceftriaxona Resistencia a las Cefalosporinas Gonorrea Humanos Pruebas de Sensibilidad Microbiana Tipificación Molecular Neisseria gonorrhoeae Filogenia Vigilancia de Guardia Análisis de Secuencia de ADN |
topic |
Antibacterianos Argentina Cefixima Ceftriaxona Resistencia a las Cefalosporinas Gonorrea Humanos Pruebas de Sensibilidad Microbiana Tipificación Molecular Neisseria gonorrhoeae Filogenia Vigilancia de Guardia Análisis de Secuencia de ADN |
dc.description.none.fl_txt_mv |
Fil: Gianecini, Ricardo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. Fil: Romero, Maria de Las Mercedes. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. Fil: Oviedo, Claudia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. Fil: Vacchino, Martin. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. Fil: Galarza, Patricia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. Background: The emergence of Neisseria gonorrhoeae strains with decreased susceptibility to cephalosporins represents a major concern globally. The aim of this study was to examine the phenotypic and molecular characteristics of N. gonorrhoeae isolates with decreased susceptibility to ceftriaxone and cefixime in Argentina. Methods: A total of 1987 isolates were collected during 2009 and 2013. The susceptibility to penicillin G, tetracycline, ciprofloxacin, cefixime, ceftriaxone, and azithromycin was determined using the agar dilution method. The major extended-spectrum cephalosporin resistance determinants (penA, mtrR, and porB1b) were sequenced in 42 N. gonorrhoeae isolates that showed decreased susceptibility to ceftriaxone (minimum inhibitory concentration [MIC], 0.06-0.125 mg/L) and cefixime (MIC, 0.125-0.25 mg/L). Genotyping by N. gonorrhoeae multiantigen sequence typing (NG-MAST) was performed. Results: Between 2009 and 2013, there was a shift in the modal MICs for ceftriaxone. Among the 42 isolates exhibiting decreased susceptibility to ceftriaxone and cefixime, 95.2% were resistant to penicillin G, 95.2% to tetracycline, 97.6% to ciprofloxacin, and 33.3% to azithromycin. Thirty-five (83.3%) of the 42 isolates had a mosaic penA allele XXXIV, which has been previously associated with resistance to ceftriaxone and cefixime as well as treatment failures. The isolates that contained the mosaic penicillin-binding protein 2 (PBP2) XXXIV were associated with NG-MAST ST1407 or closely related genotypes. Conclusions: In Argentina, N. gonorrhoeae isolates with decreased susceptibility to cefixime and ceftriaxone have now emerged, mostly due to the introduction of the internationally spread multidrug-resistant NG-MAST ST1407. |
description |
Fil: Gianecini, Ricardo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06 |
dc.type.none.fl_str_mv |
info:ar-repo/semantics/articulo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sgc.anlis.gob.ar/handle/123456789/1813 10.1097/OLQ.0000000000000603 |
url |
http://sgc.anlis.gob.ar/handle/123456789/1813 |
identifier_str_mv |
10.1097/OLQ.0000000000000603 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
#PLACEHOLDER_PARENT_METADATA_VALUE# datasets Sexually transmitted diseases |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Lippincott, Williams & Wilkins |
publisher.none.fl_str_mv |
Lippincott, Williams & Wilkins |
dc.source.none.fl_str_mv |
Sexually Transmitted Diseases 2017; 44(6):351-355 reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" instacron:ANLIS |
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Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
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Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
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Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" |
instacron_str |
ANLIS |
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ANLIS |
repository.name.fl_str_mv |
Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" |
repository.mail.fl_str_mv |
biblioteca@anlis.gov.ar |
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1844621857050853376 |
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12.559606 |