Distribution of HPV-16 variants among isolates from Paraguayan women with different grades of cervical lesion

• Autores: Mendoza, Laura Patricia; Picconi, María Alejandra; Mirazo, Santiago; Mongelos, Pamela; Gimenez, Graciela; Basiletti, Jorge; Arbiza, Juan
• Año de publicación: 2013
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: International Journal of Gynecology & Obstetrics 2013; 122(1):44-7
• Materia: Adolescente; Adulto; Anciano; Sitios de Unión; Neoplasia Intraepitelial Cervical; Femenino; Papillomavirus Humano 16; Humanos; Persona de Mediana Edad; Nucleótidos; Infecciones por Papillomavirus; Paraguay; Reacción en Cadena de la Polimerasa; Análisis de Secuencia de ADN; Factores de Transcripción; Neoplasias del Cuello Uterino; Adulto Joven
• Institución: Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"

Fil: Mendoza, Laura. National University of Asunción. Department of Public Health and Epidemiology. Health Sciences Research Institute; Paraguay.
Fil: Picconi, María A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Mirazo, Santiago. University of the Republic. Faculty of Sciences. Virology Section; Uruguay.
Fil: Mongelós, Pamela. National University of Asunción. Department of Public Health and Epidemiology. Health Sciences Research Institute; Paraguay.
Fil: Giménez, Graciela. National University of Asunción. Department of Public Health and Epidemiology. Health Sciences Research Institute; Paraguay.
Fil: Basiletti, Jorge. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
Fil: Arbiza, Juan. University of the Republic. Faculty of Sciences. Virology Section; Uruguay.
Objective: To determine the distribution of HPV-16 variants among Paraguayan women with different grades of cervical lesions. Methods: Sixty-seven HPV-16-positive cervical samples obtained from women attending health centers in Paraguay between March 2007 and April 2009 were examined, including 29 low-grade squamous intraepithelial lesion (LSIL), 29 high-grade squamous intraepithelial lesion (HSIL), 4 cervical cancer, and 5 normal cytology samples. The specimens were analyzed by PCR-directed sequencing of a 364-bp fragment of the long control region of HPV-16, and a phylogenetic tree was compiled with MEGA 5.0 software. Results: Most HPV-16 variants belonged to the European branch (82%); these variants were detected among 25 of 29 women with LSIL, 22 of 29 women with HSIL, 3 of 4 women with cervical cancer, and all women with normal cytology. Two isolates yielded new variants of the European branch with nucleotide substitutions at positions A7752C and A7810T. Non-European variants, such as African type 1 (1.5%) and Asian-American (16.5%), were detected only among women with cervical lesions (4/29, LSIL; 6/29, HSIL; 1/29, cervical cancer). These variants had at least 6 nucleotide substitutions adjacent to or within transcription factor binding sites. Conclusion: All branches of HPV-16 variants were detected among Paraguayan women with cervical lesions.