Aldo-keto reductase and alcohol dehydrogenase contribute to benznidazole natural resistance in Trypanosoma cruzi

• Autores: González, Laura; García-Huertas, Paola; Triana Chavez, Omar; García, Gabriela Andrea; Murta, Silvane Maria Fonseca; Mejia Jaramillo, Ana M.
• Año de publicación: 2017
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: Enfermedad de Chagas; Resistencia a Medicamentos; Aldo-Ceto Reductasas
• Institución: Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"

Fil: Gonzalez, Laura. Universidad de Antioquia. Grupo de Biología y Control de Enfermedades Infecciosas-BCEI; Colombia.
Fil: García-Huertas, Paola. Universidad de Antioquia. Grupo de Biología y Control de Enfermedades Infecciosas-BCEI; Colombia.
Fil: Triana Chavez, Omar. Universidad de Antioquia. Grupo de Biología y Control de Enfermedades Infecciosas-BCEI; Colombia.
Fil: García, Gabriela Andrea. ANLIS Dr. C.G.Malbrán. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben"; Argentina.
Fil: Murta, Silvane Maria Fonseca. Centro de pesquisas Rene Rachoy; Brasil.
Fil: Mejía Jaramillo, Ana M. Universidad de Antioquia. Grupo de Biología y Control de Enfermedades Infecciosas-BCEI; Colombia.
The improvement of Chagas disease treatment is focused not only on the development of new drugs but also in understanding mechanisms of action and resistance to drugs conventionally used. Thus, some strategies aim to detect specific changes in proteins between sensitive and resistant parasites and to evaluate the role played in these processes by functional genomics. In this work, we used a natural Trypanosoma cruzi population resistant to benznidazole, which has clones with different susceptibilities to this drug without alterations in the NTR I gene. Using 2DE-gel electrophoresis, the aldo-keto reductase and the alcohol dehydrogenase proteins were found up regulated in the natural resistant clone and therefore their possible role in the resistance to benznidazole and glyoxal was investigated. Both genes were overexpressed in a drug sensitive T. cruzi clone and the biological changes in response to these compounds were evaluated. The results showed that the overexpression of these proteins enhances resistance to benznidazole and glyoxal in T. cruzi. Moreover, a decrease in mitochondrial and cell membrane damage was observed, accompanied by a drop in the intracellular concentration of reactive oxygen species after treatment. Our results suggest that these proteins are involved in the mechanism of action of benznidazole.