Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples

Autores
Kucinskaite-Kodze, Indre; Petraityte-Burneikiene, Rasa; Zvirbliene, Aurelija; Hjelle, Brian; Medina, Rafael A.; Gedvilaite, Alma; Razanskiene, Ausra; Schmidt-Chanasit, Jonas; Mertens, Marc; Padula, Paula; Sasnauskas, Kestutis; Ulrich, Rainer G.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Kucinskaite-Kodze, Indre. Vilnius University. Institute of Biotechnology; Lituania.
Fil: Petraityte-Burneikiene, Rasa. Vilnius University. Institute of Biotechnology; Lituania.
Fil: Zvirbliene, Aurelija. Vilnius University. Institute of Biotechnology; Lituania.
Fil: Hjelle, Brian. University of New Mexico School of Medicine. Department of Pathology; Estados Unidos.
Fil: Medina, Rafael A. University of New Mexico School of Medicine. Department of Pathology; Estados Unidos.
Fil: Gedvilaite, Alma. Vilnius University. Institute of Biotechnology; Lituania.
Fil: Razanskiene, Ausra. Vilnius University. Institute of Biotechnology; Lituania.
Fil: Schmidt-Chanasit, Jonas. Federal Research Institute for Animal Health. Institute of Epidemiology; Alemania.
Fil: Mertens, Marc. Federal Research Institute for Animal Health. Institute of Epidemiology; Alemania.
Fil: Padula, Paula. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina.
Fil: Sasnauskas, Kestutis. Vilnius University. Institute of Biotechnology; Lituania.
Fil: Ulrich, Rainer G. Federal Research Institute for Animal Health. Institute of Epidemiology; Alemania.
Monoclonal antibodies are important tools for various applications in hantavirus diagnostics. Recently, we generated Puumala virus (PUUV)-reactive monoclonal antibodies (mAbs) by immunisation of mice with chimeric polyomavirus-derived virus-like particles (VLPs) harbouring the 120-amino-acid-long amino-terminal region of the PUUV nucleocapsid (N) protein. Here, we describe the generation of two mAbs by co-immunisation of mice with hexahistidine-tagged full-length N proteins of Sin Nombre virus (SNV) and Andes virus (ANDV), their characterization by different immunoassays and comparison with the previously generated mAbs raised against a segment of PUUV N protein inserted into VLPs. All of the mAbs reacted strongly in ELISA and western blot tests with the antigens used for immunization and cross-reacted to varying extents with N proteins of other hantaviruses. All mAbs raised against a segment of the PUUV N protein presented on chimeric VLPs and both mAbs raised against the full-length AND/SNV N protein reacted with Vero cells infected with different hantaviruses. The reactivity of mAbs with native viral nucleocapsids was also confirmed by their reactivity in immunohistochemistry assays with kidney tissue specimens from experimentally SNV-infected rodents and human heart tissue specimens from hantavirus cardiopulmonary syndrome patients. Therefore, the described mAbs represent useful tools for the immunodetection of hantavirus infection.
Fuente
Archives of Virology, 2011, 156(3), 443-456.
Materia
Anticuerpos Monoclonales
Hantavirus
Inmunohistoquímica
Roedores
Humanos
Nivel de accesibilidad
acceso abierto
Condiciones de uso
none
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:123456789/385
Acceder
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oai_identifier_str oai:sgc.anlis.gob.ar:123456789/385
network_acronym_str SGCANLIS
repository_id_str a
network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samplesKucinskaite-Kodze, IndrePetraityte-Burneikiene, RasaZvirbliene, AurelijaHjelle, BrianMedina, Rafael A.Gedvilaite, AlmaRazanskiene, AusraSchmidt-Chanasit, JonasMertens, MarcPadula, PaulaSasnauskas, KestutisUlrich, Rainer G.Anticuerpos MonoclonalesHantavirusInmunohistoquímicaRoedoresHumanosFil: Kucinskaite-Kodze, Indre. Vilnius University. Institute of Biotechnology; Lituania.Fil: Petraityte-Burneikiene, Rasa. Vilnius University. Institute of Biotechnology; Lituania.Fil: Zvirbliene, Aurelija. Vilnius University. Institute of Biotechnology; Lituania.Fil: Hjelle, Brian. University of New Mexico School of Medicine. Department of Pathology; Estados Unidos.Fil: Medina, Rafael A. University of New Mexico School of Medicine. Department of Pathology; Estados Unidos.Fil: Gedvilaite, Alma. Vilnius University. Institute of Biotechnology; Lituania.Fil: Razanskiene, Ausra. Vilnius University. Institute of Biotechnology; Lituania.Fil: Schmidt-Chanasit, Jonas. Federal Research Institute for Animal Health. Institute of Epidemiology; Alemania.Fil: Mertens, Marc. Federal Research Institute for Animal Health. Institute of Epidemiology; Alemania.Fil: Padula, Paula. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina.Fil: Sasnauskas, Kestutis. Vilnius University. Institute of Biotechnology; Lituania.Fil: Ulrich, Rainer G. Federal Research Institute for Animal Health. Institute of Epidemiology; Alemania.Monoclonal antibodies are important tools for various applications in hantavirus diagnostics. Recently, we generated Puumala virus (PUUV)-reactive monoclonal antibodies (mAbs) by immunisation of mice with chimeric polyomavirus-derived virus-like particles (VLPs) harbouring the 120-amino-acid-long amino-terminal region of the PUUV nucleocapsid (N) protein. Here, we describe the generation of two mAbs by co-immunisation of mice with hexahistidine-tagged full-length N proteins of Sin Nombre virus (SNV) and Andes virus (ANDV), their characterization by different immunoassays and comparison with the previously generated mAbs raised against a segment of PUUV N protein inserted into VLPs. All of the mAbs reacted strongly in ELISA and western blot tests with the antigens used for immunization and cross-reacted to varying extents with N proteins of other hantaviruses. All mAbs raised against a segment of the PUUV N protein presented on chimeric VLPs and both mAbs raised against the full-length AND/SNV N protein reacted with Vero cells infected with different hantaviruses. The reactivity of mAbs with native viral nucleocapsids was also confirmed by their reactivity in immunohistochemistry assays with kidney tissue specimens from experimentally SNV-infected rodents and human heart tissue specimens from hantavirus cardiopulmonary syndrome patients. Therefore, the described mAbs represent useful tools for the immunodetection of hantavirus infection.2011info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1432-8798http://sgc.anlis.gob.ar/handle/123456789/385http://link.springer.com/article/10.1007%2Fs00705-010-0879-6Archives of Virology, 2011, 156(3), 443-456.reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISArchives of virologynoneinfo:eu-repo/semantics/openAccesseng2025-09-29T14:29:59Zoai:sgc.anlis.gob.ar:123456789/385Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-29 14:30:00.443Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples
title Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples
spellingShingle Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples
Kucinskaite-Kodze, Indre
Anticuerpos Monoclonales
Hantavirus
Inmunohistoquímica
Roedores
Humanos
title_short Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples
title_full Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples
title_fullStr Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples
title_full_unstemmed Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples
title_sort Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples
dc.creator.none.fl_str_mv Kucinskaite-Kodze, Indre
Petraityte-Burneikiene, Rasa
Zvirbliene, Aurelija
Hjelle, Brian
Medina, Rafael A.
Gedvilaite, Alma
Razanskiene, Ausra
Schmidt-Chanasit, Jonas
Mertens, Marc
Padula, Paula
Sasnauskas, Kestutis
Ulrich, Rainer G.
author Kucinskaite-Kodze, Indre
author_facet Kucinskaite-Kodze, Indre
Petraityte-Burneikiene, Rasa
Zvirbliene, Aurelija
Hjelle, Brian
Medina, Rafael A.
Gedvilaite, Alma
Razanskiene, Ausra
Schmidt-Chanasit, Jonas
Mertens, Marc
Padula, Paula
Sasnauskas, Kestutis
Ulrich, Rainer G.
author_role author
author2 Petraityte-Burneikiene, Rasa
Zvirbliene, Aurelija
Hjelle, Brian
Medina, Rafael A.
Gedvilaite, Alma
Razanskiene, Ausra
Schmidt-Chanasit, Jonas
Mertens, Marc
Padula, Paula
Sasnauskas, Kestutis
Ulrich, Rainer G.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Anticuerpos Monoclonales
Hantavirus
Inmunohistoquímica
Roedores
Humanos
topic Anticuerpos Monoclonales
Hantavirus
Inmunohistoquímica
Roedores
Humanos
dc.description.none.fl_txt_mv Fil: Kucinskaite-Kodze, Indre. Vilnius University. Institute of Biotechnology; Lituania.
Fil: Petraityte-Burneikiene, Rasa. Vilnius University. Institute of Biotechnology; Lituania.
Fil: Zvirbliene, Aurelija. Vilnius University. Institute of Biotechnology; Lituania.
Fil: Hjelle, Brian. University of New Mexico School of Medicine. Department of Pathology; Estados Unidos.
Fil: Medina, Rafael A. University of New Mexico School of Medicine. Department of Pathology; Estados Unidos.
Fil: Gedvilaite, Alma. Vilnius University. Institute of Biotechnology; Lituania.
Fil: Razanskiene, Ausra. Vilnius University. Institute of Biotechnology; Lituania.
Fil: Schmidt-Chanasit, Jonas. Federal Research Institute for Animal Health. Institute of Epidemiology; Alemania.
Fil: Mertens, Marc. Federal Research Institute for Animal Health. Institute of Epidemiology; Alemania.
Fil: Padula, Paula. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina.
Fil: Sasnauskas, Kestutis. Vilnius University. Institute of Biotechnology; Lituania.
Fil: Ulrich, Rainer G. Federal Research Institute for Animal Health. Institute of Epidemiology; Alemania.
Monoclonal antibodies are important tools for various applications in hantavirus diagnostics. Recently, we generated Puumala virus (PUUV)-reactive monoclonal antibodies (mAbs) by immunisation of mice with chimeric polyomavirus-derived virus-like particles (VLPs) harbouring the 120-amino-acid-long amino-terminal region of the PUUV nucleocapsid (N) protein. Here, we describe the generation of two mAbs by co-immunisation of mice with hexahistidine-tagged full-length N proteins of Sin Nombre virus (SNV) and Andes virus (ANDV), their characterization by different immunoassays and comparison with the previously generated mAbs raised against a segment of PUUV N protein inserted into VLPs. All of the mAbs reacted strongly in ELISA and western blot tests with the antigens used for immunization and cross-reacted to varying extents with N proteins of other hantaviruses. All mAbs raised against a segment of the PUUV N protein presented on chimeric VLPs and both mAbs raised against the full-length AND/SNV N protein reacted with Vero cells infected with different hantaviruses. The reactivity of mAbs with native viral nucleocapsids was also confirmed by their reactivity in immunohistochemistry assays with kidney tissue specimens from experimentally SNV-infected rodents and human heart tissue specimens from hantavirus cardiopulmonary syndrome patients. Therefore, the described mAbs represent useful tools for the immunodetection of hantavirus infection.
description Fil: Kucinskaite-Kodze, Indre. Vilnius University. Institute of Biotechnology; Lituania.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 1432-8798
http://sgc.anlis.gob.ar/handle/123456789/385
http://link.springer.com/article/10.1007%2Fs00705-010-0879-6
identifier_str_mv 1432-8798
url http://sgc.anlis.gob.ar/handle/123456789/385
http://link.springer.com/article/10.1007%2Fs00705-010-0879-6
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Archives of virology
dc.rights.none.fl_str_mv none
info:eu-repo/semantics/openAccess
rights_invalid_str_mv none
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Archives of Virology, 2011, 156(3), 443-456.
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
collection Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
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score 12.559606

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