Susceptibility Patterns and Molecular Identification of Trichosporon Species

Autores
Rodríguez-Tudela, Juan L.; Diaz-Guerra, Teresa M.; Mellado, Emilia; Cano, Virginia; Tapia, Cecilia; Perkins, Alexander; Gomez-Lopez, Alicia; Rodero, Laura; Cuenca-Estrella, Manuel
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Rodriguez-Tudela, Juan L. Instituto de Salud Carlos III. Servicio de Micología; España.
Fil: Diaz-Guerra, Teresa M. Instituto de Salud Carlos III. Servicio de Micología; España.
Fil: Mellado, Emilia. Instituto de Salud Carlos III. Servicio de Micología; España.
Fil: Cano, Virginia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.
Fil: Tapia, Cecilia. Universidad de Chile. Programa de Microbiología y Micología; Chile.
Fil: Perkins, Alexander. Instituto de Salud Carlos III. Servicio de Micología; España.
Fil: Gomez-Lopez, Alicia. Instituto de Salud Carlos III. Servicio de Micología; España.
Fil: Rodero, Laura. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.
Fil: Cuenca-Estrella, Manuel. Instituto de Salud Carlos III. Servicio de Micología; España.
The physiological patterns, the sequence polymorphisms of the internal transcriber spacer (ITS), and intergenic spacer regions (IGS) of the rRNA genes and the antifungal susceptibility profile were evaluated for their ability to identify Trichosporon spp. and their specificity for the identification of 49 clinical isolates of Trichosporon spp. Morphological and biochemical methodologies were unable to differentiate among the Trichosporon species. ITS sequencing was also unable to differentiate several species. However, IGS1 sequencing unambiguously identified all Trichosporon isolates. Following the results of DNA-based identification, Trichosporon asahii was the species most frequently isolated from deep sites (15 of 25 strains; 60%). In the main, other Trichosporon species were recovered from cutaneous samples. The majority of T. asahii, T. faecale, and T. coremiiforme clinical isolates exhibited resistance in vitro to amphotericin B, with geometric mean (GM) MICs >4 mug/ml. The other species of Trichosporon did not show high MICs of amphotericin B, and GM MICs were <1 mug/ml. Azole agents were active in vitro against the majority of clinical strains. The most potent compound in vitro was voriconazole, with a GM MIC
Fuente
Antimicrobial Agents and Chemotherapy, 2005, 49(10), 4026–4034.
Materia
Trichosporon
Farmacorresistencia Fúngica
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:Publications/123456789/220
Acceder
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network_acronym_str SGCANLIS
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network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling Susceptibility Patterns and Molecular Identification of Trichosporon SpeciesRodríguez-Tudela, Juan L.Diaz-Guerra, Teresa M.Mellado, EmiliaCano, VirginiaTapia, CeciliaPerkins, AlexanderGomez-Lopez, AliciaRodero, LauraCuenca-Estrella, ManuelTrichosporonFarmacorresistencia FúngicaFil: Rodriguez-Tudela, Juan L. Instituto de Salud Carlos III. Servicio de Micología; España.Fil: Diaz-Guerra, Teresa M. Instituto de Salud Carlos III. Servicio de Micología; España.Fil: Mellado, Emilia. Instituto de Salud Carlos III. Servicio de Micología; España.Fil: Cano, Virginia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.Fil: Tapia, Cecilia. Universidad de Chile. Programa de Microbiología y Micología; Chile.Fil: Perkins, Alexander. Instituto de Salud Carlos III. Servicio de Micología; España.Fil: Gomez-Lopez, Alicia. Instituto de Salud Carlos III. Servicio de Micología; España.Fil: Rodero, Laura. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.Fil: Cuenca-Estrella, Manuel. Instituto de Salud Carlos III. Servicio de Micología; España.The physiological patterns, the sequence polymorphisms of the internal transcriber spacer (ITS), and intergenic spacer regions (IGS) of the rRNA genes and the antifungal susceptibility profile were evaluated for their ability to identify Trichosporon spp. and their specificity for the identification of 49 clinical isolates of Trichosporon spp. Morphological and biochemical methodologies were unable to differentiate among the Trichosporon species. ITS sequencing was also unable to differentiate several species. However, IGS1 sequencing unambiguously identified all Trichosporon isolates. Following the results of DNA-based identification, Trichosporon asahii was the species most frequently isolated from deep sites (15 of 25 strains; 60%). In the main, other Trichosporon species were recovered from cutaneous samples. The majority of T. asahii, T. faecale, and T. coremiiforme clinical isolates exhibited resistance in vitro to amphotericin B, with geometric mean (GM) MICs >4 mug/ml. The other species of Trichosporon did not show high MICs of amphotericin B, and GM MICs were <1 mug/ml. Azole agents were active in vitro against the majority of clinical strains. The most potent compound in vitro was voriconazole, with a GM MIC </=0.14 mug/ml. The sequencing of IGS correctly identified Trichosporon isolates; however, this technique is not available in many clinical laboratories, and strains should be dispatched to reference centers where these complex methods are available. Therefore, it seems to be more practical to perform antifungal susceptibility testing of all isolates belonging to Trichosporon spp., since correct identification could take several weeks, delaying the indication of an antifungal agent which exhibits activity against the infectious strain.American Society for Microbiology2005-10info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1098-6596http://sgc.anlis.gob.ar/handle/123456789/220http://aac.asm.org/content/49/10/4026.full.pdf+html10.1128-AAC.49.10.4026-4034.2005 Free PMC articleAntimicrobial Agents and Chemotherapy, 2005, 49(10), 4026–4034.reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISAntimicrobial agents and chemotherapyenginfo:eu-repo/semantics/openAccess2025-09-04T11:15:23Zoai:sgc.anlis.gob.ar:Publications/123456789/220Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-04 11:15:23.744Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv Susceptibility Patterns and Molecular Identification of Trichosporon Species
title Susceptibility Patterns and Molecular Identification of Trichosporon Species
spellingShingle Susceptibility Patterns and Molecular Identification of Trichosporon Species
Rodríguez-Tudela, Juan L.
Trichosporon
Farmacorresistencia Fúngica
title_short Susceptibility Patterns and Molecular Identification of Trichosporon Species
title_full Susceptibility Patterns and Molecular Identification of Trichosporon Species
title_fullStr Susceptibility Patterns and Molecular Identification of Trichosporon Species
title_full_unstemmed Susceptibility Patterns and Molecular Identification of Trichosporon Species
title_sort Susceptibility Patterns and Molecular Identification of Trichosporon Species
dc.creator.none.fl_str_mv Rodríguez-Tudela, Juan L.
Diaz-Guerra, Teresa M.
Mellado, Emilia
Cano, Virginia
Tapia, Cecilia
Perkins, Alexander
Gomez-Lopez, Alicia
Rodero, Laura
Cuenca-Estrella, Manuel
author Rodríguez-Tudela, Juan L.
author_facet Rodríguez-Tudela, Juan L.
Diaz-Guerra, Teresa M.
Mellado, Emilia
Cano, Virginia
Tapia, Cecilia
Perkins, Alexander
Gomez-Lopez, Alicia
Rodero, Laura
Cuenca-Estrella, Manuel
author_role author
author2 Diaz-Guerra, Teresa M.
Mellado, Emilia
Cano, Virginia
Tapia, Cecilia
Perkins, Alexander
Gomez-Lopez, Alicia
Rodero, Laura
Cuenca-Estrella, Manuel
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Trichosporon
Farmacorresistencia Fúngica
topic Trichosporon
Farmacorresistencia Fúngica
dc.description.none.fl_txt_mv Fil: Rodriguez-Tudela, Juan L. Instituto de Salud Carlos III. Servicio de Micología; España.
Fil: Diaz-Guerra, Teresa M. Instituto de Salud Carlos III. Servicio de Micología; España.
Fil: Mellado, Emilia. Instituto de Salud Carlos III. Servicio de Micología; España.
Fil: Cano, Virginia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.
Fil: Tapia, Cecilia. Universidad de Chile. Programa de Microbiología y Micología; Chile.
Fil: Perkins, Alexander. Instituto de Salud Carlos III. Servicio de Micología; España.
Fil: Gomez-Lopez, Alicia. Instituto de Salud Carlos III. Servicio de Micología; España.
Fil: Rodero, Laura. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.
Fil: Cuenca-Estrella, Manuel. Instituto de Salud Carlos III. Servicio de Micología; España.
The physiological patterns, the sequence polymorphisms of the internal transcriber spacer (ITS), and intergenic spacer regions (IGS) of the rRNA genes and the antifungal susceptibility profile were evaluated for their ability to identify Trichosporon spp. and their specificity for the identification of 49 clinical isolates of Trichosporon spp. Morphological and biochemical methodologies were unable to differentiate among the Trichosporon species. ITS sequencing was also unable to differentiate several species. However, IGS1 sequencing unambiguously identified all Trichosporon isolates. Following the results of DNA-based identification, Trichosporon asahii was the species most frequently isolated from deep sites (15 of 25 strains; 60%). In the main, other Trichosporon species were recovered from cutaneous samples. The majority of T. asahii, T. faecale, and T. coremiiforme clinical isolates exhibited resistance in vitro to amphotericin B, with geometric mean (GM) MICs >4 mug/ml. The other species of Trichosporon did not show high MICs of amphotericin B, and GM MICs were <1 mug/ml. Azole agents were active in vitro against the majority of clinical strains. The most potent compound in vitro was voriconazole, with a GM MIC </=0.14 mug/ml. The sequencing of IGS correctly identified Trichosporon isolates; however, this technique is not available in many clinical laboratories, and strains should be dispatched to reference centers where these complex methods are available. Therefore, it seems to be more practical to perform antifungal susceptibility testing of all isolates belonging to Trichosporon spp., since correct identification could take several weeks, delaying the indication of an antifungal agent which exhibits activity against the infectious strain.
description Fil: Rodriguez-Tudela, Juan L. Instituto de Salud Carlos III. Servicio de Micología; España.
publishDate 2005
dc.date.none.fl_str_mv 2005-10
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 1098-6596
http://sgc.anlis.gob.ar/handle/123456789/220
http://aac.asm.org/content/49/10/4026.full.pdf+html
10.1128-AAC.49.10.4026-4034.2005 Free PMC article
identifier_str_mv 1098-6596
10.1128-AAC.49.10.4026-4034.2005 Free PMC article
url http://sgc.anlis.gob.ar/handle/123456789/220
http://aac.asm.org/content/49/10/4026.full.pdf+html
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Antimicrobial agents and chemotherapy
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv Antimicrobial Agents and Chemotherapy, 2005, 49(10), 4026–4034.
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
collection Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
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score 12.623145

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