Evaluation of commercial enzyme-linked immunosorbent assay kits for detection of tuberculosis in Argentinean population

Autores
Imaz, María Susana; Comini, Marcelo Alberto; Zerbini, Elsa V.; Sequeira, María Delfina; Latini, Omar A.; Claus, Juan Daniel; Singh, Mahavir
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pathozyme-Myco G (Myco G), M, A, and TB complex plus (Omega Diagnostics Ltd., Alloa, Scotland) were evaluated for the serological diagnosis of pulmonary tuberculosis (TB) in an Argentinean population. Sera from 58 patients with pulmonary TB, 24 subjects with pulmonary mycobacteriosis or mycoses (pulmonary MM group), and 45 subjects with other underlying disorders (control group) were analyzed. The sensitivities of the tests ranged from 29% (Myco M) to 82% (Myco G) in smear-positive patients (17 subjects) and from 29% (TB complex plus) to 49% (Myco G) in smear-negative patients (41 subjects). The specificities of the assays varied from 93% (Myco M) to 100% (Myco G and TB complex plus) in controls and from 62% (Myco A) to 96% (TB complex plus) in the pulmonary MM group. Overall, for the diagnosis of smear-negative patients, Myco G had the best characteristics, with a sensitivity of 49% and specificities of 100% for controls and 75% for the pulmonary MM group; after its combination with TB complex plus, its sensitivity improved to 59%. Nevertheless, despite its relatively poor capacity to discriminate between pulmonary TB and pulmonary MM, Myco G, alone or in combination with TB complex plus, would be a useful diagnostic tool for patients with suspected pulmonary TB living in areas where the relative prevalence of pulmonary MM was low.
Fil: Imaz, María Susana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; Argentina.
Fil: Comini, Marcelo Alberto. Universidad Nacional del Litoral. Instituto de Tecnología Biológica; Argentina.
Fil: Zerbini, Elsa. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; Argentina.
Fil: Sequeira, María Delfina. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; Argentina.
Fil: Latini, Omar A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; Argentina.
Fil: Claus, Juan Daniel. Universidad Nacional del Litoral. Instituto de Tecnología Biológica; Argentina.
Fil: Singh, Mahavir. Gesellschaft fu¨r Biotechnologische Forschung mbH; Alemania.
Fuente
Journal of Clinical Microbiology, 2004, 42(2), 884–887.
Materia
Ensayo de Inmunoadsorción Enzimática
Juego de Reactivos para Diagnóstico
Reproducibilidad de Resultados
Tuberculosis Pulmonar
Argentina
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:123456789/278
Acceder
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oai_identifier_str oai:sgc.anlis.gob.ar:123456789/278
network_acronym_str SGCANLIS
repository_id_str a
network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling Evaluation of commercial enzyme-linked immunosorbent assay kits for detection of tuberculosis in Argentinean populationImaz, María SusanaComini, Marcelo AlbertoZerbini, Elsa V.Sequeira, María DelfinaLatini, Omar A.Claus, Juan DanielSingh, MahavirEnsayo de Inmunoadsorción EnzimáticaJuego de Reactivos para DiagnósticoReproducibilidad de ResultadosTuberculosis PulmonarArgentinaPathozyme-Myco G (Myco G), M, A, and TB complex plus (Omega Diagnostics Ltd., Alloa, Scotland) were evaluated for the serological diagnosis of pulmonary tuberculosis (TB) in an Argentinean population. Sera from 58 patients with pulmonary TB, 24 subjects with pulmonary mycobacteriosis or mycoses (pulmonary MM group), and 45 subjects with other underlying disorders (control group) were analyzed. The sensitivities of the tests ranged from 29% (Myco M) to 82% (Myco G) in smear-positive patients (17 subjects) and from 29% (TB complex plus) to 49% (Myco G) in smear-negative patients (41 subjects). The specificities of the assays varied from 93% (Myco M) to 100% (Myco G and TB complex plus) in controls and from 62% (Myco A) to 96% (TB complex plus) in the pulmonary MM group. Overall, for the diagnosis of smear-negative patients, Myco G had the best characteristics, with a sensitivity of 49% and specificities of 100% for controls and 75% for the pulmonary MM group; after its combination with TB complex plus, its sensitivity improved to 59%. Nevertheless, despite its relatively poor capacity to discriminate between pulmonary TB and pulmonary MM, Myco G, alone or in combination with TB complex plus, would be a useful diagnostic tool for patients with suspected pulmonary TB living in areas where the relative prevalence of pulmonary MM was low.Fil: Imaz, María Susana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; Argentina.Fil: Comini, Marcelo Alberto. Universidad Nacional del Litoral. Instituto de Tecnología Biológica; Argentina.Fil: Zerbini, Elsa. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; Argentina.Fil: Sequeira, María Delfina. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; Argentina.Fil: Latini, Omar A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; Argentina.Fil: Claus, Juan Daniel. Universidad Nacional del Litoral. Instituto de Tecnología Biológica; Argentina.Fil: Singh, Mahavir. Gesellschaft fu¨r Biotechnologische Forschung mbH; Alemania.2004info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1098-660Xhttp://jcm.asm.org/content/42/2/884.full.pdf+htmlhttp://sgc.anlis.gob.ar/handle/123456789/278Journal of Clinical Microbiology, 2004, 42(2), 884–887.reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISARGenginfo:eu-repo/semantics/openAccess2025-11-27T10:18:31Zoai:sgc.anlis.gob.ar:123456789/278Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-11-27 10:18:31.895Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv Evaluation of commercial enzyme-linked immunosorbent assay kits for detection of tuberculosis in Argentinean population
title Evaluation of commercial enzyme-linked immunosorbent assay kits for detection of tuberculosis in Argentinean population
spellingShingle Evaluation of commercial enzyme-linked immunosorbent assay kits for detection of tuberculosis in Argentinean population
Imaz, María Susana
Ensayo de Inmunoadsorción Enzimática
Juego de Reactivos para Diagnóstico
Reproducibilidad de Resultados
Tuberculosis Pulmonar
Argentina
title_short Evaluation of commercial enzyme-linked immunosorbent assay kits for detection of tuberculosis in Argentinean population
title_full Evaluation of commercial enzyme-linked immunosorbent assay kits for detection of tuberculosis in Argentinean population
title_fullStr Evaluation of commercial enzyme-linked immunosorbent assay kits for detection of tuberculosis in Argentinean population
title_full_unstemmed Evaluation of commercial enzyme-linked immunosorbent assay kits for detection of tuberculosis in Argentinean population
title_sort Evaluation of commercial enzyme-linked immunosorbent assay kits for detection of tuberculosis in Argentinean population
dc.creator.none.fl_str_mv Imaz, María Susana
Comini, Marcelo Alberto
Zerbini, Elsa V.
Sequeira, María Delfina
Latini, Omar A.
Claus, Juan Daniel
Singh, Mahavir
author Imaz, María Susana
author_facet Imaz, María Susana
Comini, Marcelo Alberto
Zerbini, Elsa V.
Sequeira, María Delfina
Latini, Omar A.
Claus, Juan Daniel
Singh, Mahavir
author_role author
author2 Comini, Marcelo Alberto
Zerbini, Elsa V.
Sequeira, María Delfina
Latini, Omar A.
Claus, Juan Daniel
Singh, Mahavir
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ensayo de Inmunoadsorción Enzimática
Juego de Reactivos para Diagnóstico
Reproducibilidad de Resultados
Tuberculosis Pulmonar
Argentina
topic Ensayo de Inmunoadsorción Enzimática
Juego de Reactivos para Diagnóstico
Reproducibilidad de Resultados
Tuberculosis Pulmonar
Argentina
dc.description.none.fl_txt_mv Pathozyme-Myco G (Myco G), M, A, and TB complex plus (Omega Diagnostics Ltd., Alloa, Scotland) were evaluated for the serological diagnosis of pulmonary tuberculosis (TB) in an Argentinean population. Sera from 58 patients with pulmonary TB, 24 subjects with pulmonary mycobacteriosis or mycoses (pulmonary MM group), and 45 subjects with other underlying disorders (control group) were analyzed. The sensitivities of the tests ranged from 29% (Myco M) to 82% (Myco G) in smear-positive patients (17 subjects) and from 29% (TB complex plus) to 49% (Myco G) in smear-negative patients (41 subjects). The specificities of the assays varied from 93% (Myco M) to 100% (Myco G and TB complex plus) in controls and from 62% (Myco A) to 96% (TB complex plus) in the pulmonary MM group. Overall, for the diagnosis of smear-negative patients, Myco G had the best characteristics, with a sensitivity of 49% and specificities of 100% for controls and 75% for the pulmonary MM group; after its combination with TB complex plus, its sensitivity improved to 59%. Nevertheless, despite its relatively poor capacity to discriminate between pulmonary TB and pulmonary MM, Myco G, alone or in combination with TB complex plus, would be a useful diagnostic tool for patients with suspected pulmonary TB living in areas where the relative prevalence of pulmonary MM was low.
Fil: Imaz, María Susana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; Argentina.
Fil: Comini, Marcelo Alberto. Universidad Nacional del Litoral. Instituto de Tecnología Biológica; Argentina.
Fil: Zerbini, Elsa. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; Argentina.
Fil: Sequeira, María Delfina. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; Argentina.
Fil: Latini, Omar A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; Argentina.
Fil: Claus, Juan Daniel. Universidad Nacional del Litoral. Instituto de Tecnología Biológica; Argentina.
Fil: Singh, Mahavir. Gesellschaft fu¨r Biotechnologische Forschung mbH; Alemania.
description Pathozyme-Myco G (Myco G), M, A, and TB complex plus (Omega Diagnostics Ltd., Alloa, Scotland) were evaluated for the serological diagnosis of pulmonary tuberculosis (TB) in an Argentinean population. Sera from 58 patients with pulmonary TB, 24 subjects with pulmonary mycobacteriosis or mycoses (pulmonary MM group), and 45 subjects with other underlying disorders (control group) were analyzed. The sensitivities of the tests ranged from 29% (Myco M) to 82% (Myco G) in smear-positive patients (17 subjects) and from 29% (TB complex plus) to 49% (Myco G) in smear-negative patients (41 subjects). The specificities of the assays varied from 93% (Myco M) to 100% (Myco G and TB complex plus) in controls and from 62% (Myco A) to 96% (TB complex plus) in the pulmonary MM group. Overall, for the diagnosis of smear-negative patients, Myco G had the best characteristics, with a sensitivity of 49% and specificities of 100% for controls and 75% for the pulmonary MM group; after its combination with TB complex plus, its sensitivity improved to 59%. Nevertheless, despite its relatively poor capacity to discriminate between pulmonary TB and pulmonary MM, Myco G, alone or in combination with TB complex plus, would be a useful diagnostic tool for patients with suspected pulmonary TB living in areas where the relative prevalence of pulmonary MM was low.
publishDate 2004
dc.date.none.fl_str_mv 2004
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 1098-660X
http://jcm.asm.org/content/42/2/884.full.pdf+html
http://sgc.anlis.gob.ar/handle/123456789/278
identifier_str_mv 1098-660X
url http://jcm.asm.org/content/42/2/884.full.pdf+html
http://sgc.anlis.gob.ar/handle/123456789/278
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv ARG
dc.source.none.fl_str_mv Journal of Clinical Microbiology, 2004, 42(2), 884–887.
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
collection Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
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score 12.764688

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