Trypanosoma cruzi: biological characterization of a isolate from an endemic area and its susceptibility to conventional drugs

Autores
Grosso, Noelia L.; Bua, Jacqueline; Perrone, Alina E.; Gonzalez, Mariela N.; Bustos, Patricia L.; Postan, Miriam; Fichera, Laura E.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Grosso, Noelia L. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
Fil: Bua, Jacqueline. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
Fil: Perrone, Alina E. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
Fil: Gonzalez, Mariela N. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
Fil: Bustos, Patricia L. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
Fil: Postan, Miriam. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
Fil: Fichera, Laura E. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
We describe some biological and molecular characteristics of a Trypanosoma cruzi isolate derived from a Triatomine captured in Nicaragua. PCR based typification showed that this isolate, named Nicaragua, belonged to the lineage Tc I. Nicaragua infected culture cells were treated with allopurinol, showing different behavior according to the cellular compartment, being cardiomyocyte primary cultures more resistant to this drug. The course of the infection in a mice experimental model and its susceptibility to benznidazole and allopurinol was analyzed. In benznidazole treatment, mice reverted the high lethal effect of parasites during the acute infection, however, a few parasites were detected in the heart of 88% of mice 1 year post-infection. Since T. cruzi is a heterogeneous species population it is important to study and characterize different parasites actually circulating in humans in endemic areas. In this work we show that T. cruzi Nicaragua isolate, is sensitive to early benznidazole treatment.
Fuente
Experimental Parasitology 2010; 126(2): 239-244.
Materia
Trypanosoma cruzi
Alopurinol
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:Publications/123456789/461
Acceder
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oai_identifier_str oai:sgc.anlis.gob.ar:Publications/123456789/461
network_acronym_str SGCANLIS
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network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling Trypanosoma cruzi: biological characterization of a isolate from an endemic area and its susceptibility to conventional drugsGrosso, Noelia L.Bua, JacquelinePerrone, Alina E.Gonzalez, Mariela N.Bustos, Patricia L.Postan, MiriamFichera, Laura E.Trypanosoma cruziAlopurinolFil: Grosso, Noelia L. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.Fil: Bua, Jacqueline. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.Fil: Perrone, Alina E. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.Fil: Gonzalez, Mariela N. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.Fil: Bustos, Patricia L. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.Fil: Postan, Miriam. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.Fil: Fichera, Laura E. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.We describe some biological and molecular characteristics of a Trypanosoma cruzi isolate derived from a Triatomine captured in Nicaragua. PCR based typification showed that this isolate, named Nicaragua, belonged to the lineage Tc I. Nicaragua infected culture cells were treated with allopurinol, showing different behavior according to the cellular compartment, being cardiomyocyte primary cultures more resistant to this drug. The course of the infection in a mice experimental model and its susceptibility to benznidazole and allopurinol was analyzed. In benznidazole treatment, mice reverted the high lethal effect of parasites during the acute infection, however, a few parasites were detected in the heart of 88% of mice 1 year post-infection. Since T. cruzi is a heterogeneous species population it is important to study and characterize different parasites actually circulating in humans in endemic areas. In this work we show that T. cruzi Nicaragua isolate, is sensitive to early benznidazole treatment.Elsevier2010info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://sgc.anlis.gob.ar/handle/123456789/46110.1016/j.exppara.2010.05.010Experimental Parasitology 2010; 126(2): 239-244.reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISExperimental parasitologyenginfo:eu-repo/semantics/openAccess2025-09-04T11:15:51Zoai:sgc.anlis.gob.ar:Publications/123456789/461Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-04 11:15:51.6Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv Trypanosoma cruzi: biological characterization of a isolate from an endemic area and its susceptibility to conventional drugs
title Trypanosoma cruzi: biological characterization of a isolate from an endemic area and its susceptibility to conventional drugs
spellingShingle Trypanosoma cruzi: biological characterization of a isolate from an endemic area and its susceptibility to conventional drugs
Grosso, Noelia L.
Trypanosoma cruzi
Alopurinol
title_short Trypanosoma cruzi: biological characterization of a isolate from an endemic area and its susceptibility to conventional drugs
title_full Trypanosoma cruzi: biological characterization of a isolate from an endemic area and its susceptibility to conventional drugs
title_fullStr Trypanosoma cruzi: biological characterization of a isolate from an endemic area and its susceptibility to conventional drugs
title_full_unstemmed Trypanosoma cruzi: biological characterization of a isolate from an endemic area and its susceptibility to conventional drugs
title_sort Trypanosoma cruzi: biological characterization of a isolate from an endemic area and its susceptibility to conventional drugs
dc.creator.none.fl_str_mv Grosso, Noelia L.
Bua, Jacqueline
Perrone, Alina E.
Gonzalez, Mariela N.
Bustos, Patricia L.
Postan, Miriam
Fichera, Laura E.
author Grosso, Noelia L.
author_facet Grosso, Noelia L.
Bua, Jacqueline
Perrone, Alina E.
Gonzalez, Mariela N.
Bustos, Patricia L.
Postan, Miriam
Fichera, Laura E.
author_role author
author2 Bua, Jacqueline
Perrone, Alina E.
Gonzalez, Mariela N.
Bustos, Patricia L.
Postan, Miriam
Fichera, Laura E.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Trypanosoma cruzi
Alopurinol
topic Trypanosoma cruzi
Alopurinol
dc.description.none.fl_txt_mv Fil: Grosso, Noelia L. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
Fil: Bua, Jacqueline. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
Fil: Perrone, Alina E. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
Fil: Gonzalez, Mariela N. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
Fil: Bustos, Patricia L. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
Fil: Postan, Miriam. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
Fil: Fichera, Laura E. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
We describe some biological and molecular characteristics of a Trypanosoma cruzi isolate derived from a Triatomine captured in Nicaragua. PCR based typification showed that this isolate, named Nicaragua, belonged to the lineage Tc I. Nicaragua infected culture cells were treated with allopurinol, showing different behavior according to the cellular compartment, being cardiomyocyte primary cultures more resistant to this drug. The course of the infection in a mice experimental model and its susceptibility to benznidazole and allopurinol was analyzed. In benznidazole treatment, mice reverted the high lethal effect of parasites during the acute infection, however, a few parasites were detected in the heart of 88% of mice 1 year post-infection. Since T. cruzi is a heterogeneous species population it is important to study and characterize different parasites actually circulating in humans in endemic areas. In this work we show that T. cruzi Nicaragua isolate, is sensitive to early benznidazole treatment.
description Fil: Grosso, Noelia L. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sgc.anlis.gob.ar/handle/123456789/461
10.1016/j.exppara.2010.05.010
url http://sgc.anlis.gob.ar/handle/123456789/461
identifier_str_mv 10.1016/j.exppara.2010.05.010
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Experimental parasitology
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Experimental Parasitology 2010; 126(2): 239-244.
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
collection Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
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score 12.623145

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