Isolation of five Enterobacteriaceae species harbouring blaNDM-1 and mcr-1 plasmids from a single paediatric patient

Autores
Martino, Florencia; Tijet, Nathalie; Melano, Roberto; Petroni, Alejandro; Heinz, E; De Belder, Denise; Faccone, Diego; Rapoport, Melina J.; Biondi, Estefania; Rodrigo, Veronica; Vázquez, Miryam; Pasteran, Fernando; Thomson, Nicholas R; Corso, Alejandra; Gomez, Sonia A.
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Martino, Florencia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentin
Fil: Tijet, Nathalie. Public Health Ontario Laboratories, Toronto, Ontario; Canadá.
Fil: Melano, Roberto. Public Health Ontario Laboratories, Toronto, Ontario; Canadá.
Fil: Petroni, Alejandro. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
Fil: Heinz, E. The Welcome Trust Sanger Institute, Hinxton, Cambridge; Reino Unido.
Fil: De Belder, Denise. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
Fil: Faccone, Diego. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
Fil: Rapoport, Melina J. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
Fil: Biondi, Estefania. Hospital de Niños Dr. Ricardo Gutiérrez, Ciudad Autónoma de Buenos Aires; Argentina.
Fil: Rodrigo, Veronica. Hospital de Niños Dr. Ricardo Gutiérrez, Ciudad Autónoma de Buenos Aires; Argentina.
Fil: Vázquez, Miryam. Hospital de Niños Dr. Ricardo Gutiérrez, Ciudad Autónoma de Buenos Aires; Argentina.
Fil: Pasteran, Fernando. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
Fil: Thomson, Nicholas R. The Welcome Trust Sanger Institute, Hinxton, Cambridge; Reino Unido.
Fil: Corso, Alejandra. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
Fil: Gomez, Sonia A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
In Argentina, NDM metallo-β-lactamase was first reported in 2013. By now, it has disseminated throughout the country in diverse Gram negative bacteria. Here, we report the case of a paediatric patient that underwent a 1-year hospitalisation due to erythrodermic psoriasis in 2014 and received multiple antimicrobial treatments. During his stay, five isolates were obtained from rectal swabs (rs) or blood culture (bc) suspicious of carbapenemase production: a K. quasipneumoniae subsp. quasipneumoniae (rs), Citrobacter freundii (rs), Escherichia coli (bc), Enterobacter cloacae (rs), and a Serratia marcescens (bc). The isolates were studied with broth microdilution, biparental conjugation and plasmid and whole genome sequencing (Illumina). All isolates harboured an 138,998-bp type 1 IncC plasmid that carried blaNDM-1, bleMBL, blaCMY-6, rmtC, aac(6')-Ib, and sul1 resistance genes. Additionally, the blaNDM-plasmids contained ISKpn8 an insertion sequence previously described as associated only to blaKPC. One isolate, a colistin-resistant E. coli, also carried a mcr-1-containing an IncI2 plasmid, which did not harbour additional resistance. The whole genome of K. quasipneumoniae subsp. quasipneumoniae isolate was fully sequenced. This isolate harboured, additionally to blaNDM, three plasmid-mediated quinolone resistance genes: qnrB4, qnrB52 and aac(6')-Ib-cr1. The E. cloacae isolate also harboured qnrA1. These findings alert to the underestimated horizontal dissemination of multidrug-resistant plasmids limiting treatment options with last resort antimicrobials. This research was funded by Agencia Nacional de Promoción Científica y Tecnológica - Fondo para la Investigación Científica y Tecnológica, PICT-2012-0145 to SAG. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Fuente
PLoS One 2019;14(9):e0221960
Materia
Pediatría
Enterobacteriaceae
Plásmidos
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:Publications/123456789/1531
Acceder
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network_acronym_str SGCANLIS
repository_id_str a
network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling Isolation of five Enterobacteriaceae species harbouring blaNDM-1 and mcr-1 plasmids from a single paediatric patientMartino, FlorenciaTijet, NathalieMelano, RobertoPetroni, AlejandroHeinz, EDe Belder, DeniseFaccone, DiegoRapoport, Melina J.Biondi, EstefaniaRodrigo, VeronicaVázquez, MiryamPasteran, FernandoThomson, Nicholas RCorso, AlejandraGomez, Sonia A.PediatríaEnterobacteriaceaePlásmidosFil: Martino, Florencia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; ArgentinFil: Tijet, Nathalie. Public Health Ontario Laboratories, Toronto, Ontario; Canadá.Fil: Melano, Roberto. Public Health Ontario Laboratories, Toronto, Ontario; Canadá.Fil: Petroni, Alejandro. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.Fil: Heinz, E. The Welcome Trust Sanger Institute, Hinxton, Cambridge; Reino Unido.Fil: De Belder, Denise. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.Fil: Faccone, Diego. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.Fil: Rapoport, Melina J. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.Fil: Biondi, Estefania. Hospital de Niños Dr. Ricardo Gutiérrez, Ciudad Autónoma de Buenos Aires; Argentina.Fil: Rodrigo, Veronica. Hospital de Niños Dr. Ricardo Gutiérrez, Ciudad Autónoma de Buenos Aires; Argentina.Fil: Vázquez, Miryam. Hospital de Niños Dr. Ricardo Gutiérrez, Ciudad Autónoma de Buenos Aires; Argentina.Fil: Pasteran, Fernando. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.Fil: Thomson, Nicholas R. The Welcome Trust Sanger Institute, Hinxton, Cambridge; Reino Unido.Fil: Corso, Alejandra. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.Fil: Gomez, Sonia A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.In Argentina, NDM metallo-β-lactamase was first reported in 2013. By now, it has disseminated throughout the country in diverse Gram negative bacteria. Here, we report the case of a paediatric patient that underwent a 1-year hospitalisation due to erythrodermic psoriasis in 2014 and received multiple antimicrobial treatments. During his stay, five isolates were obtained from rectal swabs (rs) or blood culture (bc) suspicious of carbapenemase production: a K. quasipneumoniae subsp. quasipneumoniae (rs), Citrobacter freundii (rs), Escherichia coli (bc), Enterobacter cloacae (rs), and a Serratia marcescens (bc). The isolates were studied with broth microdilution, biparental conjugation and plasmid and whole genome sequencing (Illumina). All isolates harboured an 138,998-bp type 1 IncC plasmid that carried blaNDM-1, bleMBL, blaCMY-6, rmtC, aac(6')-Ib, and sul1 resistance genes. Additionally, the blaNDM-plasmids contained ISKpn8 an insertion sequence previously described as associated only to blaKPC. One isolate, a colistin-resistant E. coli, also carried a mcr-1-containing an IncI2 plasmid, which did not harbour additional resistance. The whole genome of K. quasipneumoniae subsp. quasipneumoniae isolate was fully sequenced. This isolate harboured, additionally to blaNDM, three plasmid-mediated quinolone resistance genes: qnrB4, qnrB52 and aac(6')-Ib-cr1. The E. cloacae isolate also harboured qnrA1. These findings alert to the underestimated horizontal dissemination of multidrug-resistant plasmids limiting treatment options with last resort antimicrobials. This research was funded by Agencia Nacional de Promoción Científica y Tecnológica - Fondo para la Investigación Científica y Tecnológica, PICT-2012-0145 to SAG. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.2019-08-09info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1932-6203https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0221960http://sgc.anlis.gob.ar/handle/123456789/153110.1371/journal.pone.0221960PLoS One 2019;14(9):e0221960reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISPloS oneenginfo:eu-repo/semantics/openAccess2026-02-26T14:07:30Zoai:sgc.anlis.gob.ar:Publications/123456789/1531Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2026-02-26 14:07:30.853Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv Isolation of five Enterobacteriaceae species harbouring blaNDM-1 and mcr-1 plasmids from a single paediatric patient
title Isolation of five Enterobacteriaceae species harbouring blaNDM-1 and mcr-1 plasmids from a single paediatric patient
spellingShingle Isolation of five Enterobacteriaceae species harbouring blaNDM-1 and mcr-1 plasmids from a single paediatric patient
Martino, Florencia
Pediatría
Enterobacteriaceae
Plásmidos
title_short Isolation of five Enterobacteriaceae species harbouring blaNDM-1 and mcr-1 plasmids from a single paediatric patient
title_full Isolation of five Enterobacteriaceae species harbouring blaNDM-1 and mcr-1 plasmids from a single paediatric patient
title_fullStr Isolation of five Enterobacteriaceae species harbouring blaNDM-1 and mcr-1 plasmids from a single paediatric patient
title_full_unstemmed Isolation of five Enterobacteriaceae species harbouring blaNDM-1 and mcr-1 plasmids from a single paediatric patient
title_sort Isolation of five Enterobacteriaceae species harbouring blaNDM-1 and mcr-1 plasmids from a single paediatric patient
dc.creator.none.fl_str_mv Martino, Florencia
Tijet, Nathalie
Melano, Roberto
Petroni, Alejandro
Heinz, E
De Belder, Denise
Faccone, Diego
Rapoport, Melina J.
Biondi, Estefania
Rodrigo, Veronica
Vázquez, Miryam
Pasteran, Fernando
Thomson, Nicholas R
Corso, Alejandra
Gomez, Sonia A.
author Martino, Florencia
author_facet Martino, Florencia
Tijet, Nathalie
Melano, Roberto
Petroni, Alejandro
Heinz, E
De Belder, Denise
Faccone, Diego
Rapoport, Melina J.
Biondi, Estefania
Rodrigo, Veronica
Vázquez, Miryam
Pasteran, Fernando
Thomson, Nicholas R
Corso, Alejandra
Gomez, Sonia A.
author_role author
author2 Tijet, Nathalie
Melano, Roberto
Petroni, Alejandro
Heinz, E
De Belder, Denise
Faccone, Diego
Rapoport, Melina J.
Biondi, Estefania
Rodrigo, Veronica
Vázquez, Miryam
Pasteran, Fernando
Thomson, Nicholas R
Corso, Alejandra
Gomez, Sonia A.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Pediatría
Enterobacteriaceae
Plásmidos
topic Pediatría
Enterobacteriaceae
Plásmidos
dc.description.none.fl_txt_mv Fil: Martino, Florencia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentin
Fil: Tijet, Nathalie. Public Health Ontario Laboratories, Toronto, Ontario; Canadá.
Fil: Melano, Roberto. Public Health Ontario Laboratories, Toronto, Ontario; Canadá.
Fil: Petroni, Alejandro. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
Fil: Heinz, E. The Welcome Trust Sanger Institute, Hinxton, Cambridge; Reino Unido.
Fil: De Belder, Denise. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
Fil: Faccone, Diego. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
Fil: Rapoport, Melina J. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
Fil: Biondi, Estefania. Hospital de Niños Dr. Ricardo Gutiérrez, Ciudad Autónoma de Buenos Aires; Argentina.
Fil: Rodrigo, Veronica. Hospital de Niños Dr. Ricardo Gutiérrez, Ciudad Autónoma de Buenos Aires; Argentina.
Fil: Vázquez, Miryam. Hospital de Niños Dr. Ricardo Gutiérrez, Ciudad Autónoma de Buenos Aires; Argentina.
Fil: Pasteran, Fernando. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
Fil: Thomson, Nicholas R. The Welcome Trust Sanger Institute, Hinxton, Cambridge; Reino Unido.
Fil: Corso, Alejandra. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
Fil: Gomez, Sonia A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
In Argentina, NDM metallo-β-lactamase was first reported in 2013. By now, it has disseminated throughout the country in diverse Gram negative bacteria. Here, we report the case of a paediatric patient that underwent a 1-year hospitalisation due to erythrodermic psoriasis in 2014 and received multiple antimicrobial treatments. During his stay, five isolates were obtained from rectal swabs (rs) or blood culture (bc) suspicious of carbapenemase production: a K. quasipneumoniae subsp. quasipneumoniae (rs), Citrobacter freundii (rs), Escherichia coli (bc), Enterobacter cloacae (rs), and a Serratia marcescens (bc). The isolates were studied with broth microdilution, biparental conjugation and plasmid and whole genome sequencing (Illumina). All isolates harboured an 138,998-bp type 1 IncC plasmid that carried blaNDM-1, bleMBL, blaCMY-6, rmtC, aac(6')-Ib, and sul1 resistance genes. Additionally, the blaNDM-plasmids contained ISKpn8 an insertion sequence previously described as associated only to blaKPC. One isolate, a colistin-resistant E. coli, also carried a mcr-1-containing an IncI2 plasmid, which did not harbour additional resistance. The whole genome of K. quasipneumoniae subsp. quasipneumoniae isolate was fully sequenced. This isolate harboured, additionally to blaNDM, three plasmid-mediated quinolone resistance genes: qnrB4, qnrB52 and aac(6')-Ib-cr1. The E. cloacae isolate also harboured qnrA1. These findings alert to the underestimated horizontal dissemination of multidrug-resistant plasmids limiting treatment options with last resort antimicrobials. This research was funded by Agencia Nacional de Promoción Científica y Tecnológica - Fondo para la Investigación Científica y Tecnológica, PICT-2012-0145 to SAG. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
description Fil: Martino, Florencia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentin
publishDate 2019
dc.date.none.fl_str_mv 2019-08-09
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 1932-6203
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0221960
http://sgc.anlis.gob.ar/handle/123456789/1531
10.1371/journal.pone.0221960
identifier_str_mv 1932-6203
10.1371/journal.pone.0221960
url https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0221960
http://sgc.anlis.gob.ar/handle/123456789/1531
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PloS one
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv PLoS One 2019;14(9):e0221960
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
collection Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
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