Fluorescence polarization assay for diagnosis of human brucellosis

Autores
Lucero, Nidia E.; Escobar, Gabriela I.; Ayala, Sandra M.; Silva Paulo, Patricia; Nielsen, Klaus
Año de publicación
2003
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fluorescence polarization immunoassay (FPA) uses molecular rotational properties to measure antibody binding toantigendirectly. Thepotential useof thismethodwas assessed incomparison to a competitive enzyme immunoassay (CELISA) and conventional serological tests for the diagnosis of brucellosis on a total of 587 human sera. Based on 340 sera from asymptomatic blood donors with no evidence of brucellosis, the specificity of the FPAwas97·9% using a cut-off value of 72mP. Sera fromBrucella-infected patients (11Brucellamelitensis,32Brucella abortus,32Brucella suis and oneBrucellasp.) yielded a sensitivity estimate of 96·1%. In tests on 84 sera from suspected brucellosis patients, the FPA detected 80 cases. Of 87 sera from patients with probable infection, 15 were detected by both CELISA and FPA, three by CELISA only and four by FPA only. The discrepancies inbothgroups involvedserawith low, declining titres. TheFPAuses a sampleof 40 l serum, takesabout 5 min tocompleteandhasbeendemonstrated tobeaccurate for thedetectionof antibodies toB. abortus, B. melitensisandB. suisand for identifying patients suffering relapses. Because of the ease of the procedure, it could be readily adopted for use in clinical laboratories and blood banks.
Fil: Lucero, Nidia E. ANLIS Dr.C.G.Malbrán. Laboratorio de Brucelosis; Argentina.
Fil: Escobar, Gabriela I. ANLIS Dr.C.G.Malbrán. Laboratorio de Brucelosis; Argentina.
Fil: Ayala, Sandra M. ANLIS Dr.C.G.Malbrán. Laboratorio de Brucelosis; Argentina.
Fil: Silva Paulo, Patricia, Comision Nacional de Energia Atomica. Centro Atomico de Ezeiza; Argentina.
Fil: Nielsen, Klaus. Canadian Food Inspection Agency. Animal Diseases Research Institute; Canada.
Fuente
Journal of Medical Microbiology, 2003, 52(10), 883–887.
Materia
Brucelosis
Diagnóstico
Inmunoensayo de Polarización Fluorescente
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:123456789/355
Acceder
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oai_identifier_str oai:sgc.anlis.gob.ar:123456789/355
network_acronym_str SGCANLIS
repository_id_str a
network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling Fluorescence polarization assay for diagnosis of human brucellosisLucero, Nidia E.Escobar, Gabriela I.Ayala, Sandra M.Silva Paulo, PatriciaNielsen, KlausBrucelosisDiagnósticoInmunoensayo de Polarización FluorescenteFluorescence polarization immunoassay (FPA) uses molecular rotational properties to measure antibody binding toantigendirectly. Thepotential useof thismethodwas assessed incomparison to a competitive enzyme immunoassay (CELISA) and conventional serological tests for the diagnosis of brucellosis on a total of 587 human sera. Based on 340 sera from asymptomatic blood donors with no evidence of brucellosis, the specificity of the FPAwas97·9% using a cut-off value of 72mP. Sera fromBrucella-infected patients (11Brucellamelitensis,32Brucella abortus,32Brucella suis and oneBrucellasp.) yielded a sensitivity estimate of 96·1%. In tests on 84 sera from suspected brucellosis patients, the FPA detected 80 cases. Of 87 sera from patients with probable infection, 15 were detected by both CELISA and FPA, three by CELISA only and four by FPA only. The discrepancies inbothgroups involvedserawith low, declining titres. TheFPAuses a sampleof 40 l serum, takesabout 5 min tocompleteandhasbeendemonstrated tobeaccurate for thedetectionof antibodies toB. abortus, B. melitensisandB. suisand for identifying patients suffering relapses. Because of the ease of the procedure, it could be readily adopted for use in clinical laboratories and blood banks.Fil: Lucero, Nidia E. ANLIS Dr.C.G.Malbrán. Laboratorio de Brucelosis; Argentina.Fil: Escobar, Gabriela I. ANLIS Dr.C.G.Malbrán. Laboratorio de Brucelosis; Argentina.Fil: Ayala, Sandra M. ANLIS Dr.C.G.Malbrán. Laboratorio de Brucelosis; Argentina.Fil: Silva Paulo, Patricia, Comision Nacional de Energia Atomica. Centro Atomico de Ezeiza; Argentina.Fil: Nielsen, Klaus. Canadian Food Inspection Agency. Animal Diseases Research Institute; Canada.2003info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf0022-2615http://sgc.anlis.gob.ar/handle/123456789/355http://jmm.sgmjournals.org/content/52/10/883.full.pdfJournal of Medical Microbiology, 2003, 52(10), 883–887.reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISenginfo:eu-repo/semantics/openAccess2025-09-29T14:29:59Zoai:sgc.anlis.gob.ar:123456789/355Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-29 14:30:00.284Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv Fluorescence polarization assay for diagnosis of human brucellosis
title Fluorescence polarization assay for diagnosis of human brucellosis
spellingShingle Fluorescence polarization assay for diagnosis of human brucellosis
Lucero, Nidia E.
Brucelosis
Diagnóstico
Inmunoensayo de Polarización Fluorescente
title_short Fluorescence polarization assay for diagnosis of human brucellosis
title_full Fluorescence polarization assay for diagnosis of human brucellosis
title_fullStr Fluorescence polarization assay for diagnosis of human brucellosis
title_full_unstemmed Fluorescence polarization assay for diagnosis of human brucellosis
title_sort Fluorescence polarization assay for diagnosis of human brucellosis
dc.creator.none.fl_str_mv Lucero, Nidia E.
Escobar, Gabriela I.
Ayala, Sandra M.
Silva Paulo, Patricia
Nielsen, Klaus
author Lucero, Nidia E.
author_facet Lucero, Nidia E.
Escobar, Gabriela I.
Ayala, Sandra M.
Silva Paulo, Patricia
Nielsen, Klaus
author_role author
author2 Escobar, Gabriela I.
Ayala, Sandra M.
Silva Paulo, Patricia
Nielsen, Klaus
author2_role author
author
author
author
dc.subject.none.fl_str_mv Brucelosis
Diagnóstico
Inmunoensayo de Polarización Fluorescente
topic Brucelosis
Diagnóstico
Inmunoensayo de Polarización Fluorescente
dc.description.none.fl_txt_mv Fluorescence polarization immunoassay (FPA) uses molecular rotational properties to measure antibody binding toantigendirectly. Thepotential useof thismethodwas assessed incomparison to a competitive enzyme immunoassay (CELISA) and conventional serological tests for the diagnosis of brucellosis on a total of 587 human sera. Based on 340 sera from asymptomatic blood donors with no evidence of brucellosis, the specificity of the FPAwas97·9% using a cut-off value of 72mP. Sera fromBrucella-infected patients (11Brucellamelitensis,32Brucella abortus,32Brucella suis and oneBrucellasp.) yielded a sensitivity estimate of 96·1%. In tests on 84 sera from suspected brucellosis patients, the FPA detected 80 cases. Of 87 sera from patients with probable infection, 15 were detected by both CELISA and FPA, three by CELISA only and four by FPA only. The discrepancies inbothgroups involvedserawith low, declining titres. TheFPAuses a sampleof 40 l serum, takesabout 5 min tocompleteandhasbeendemonstrated tobeaccurate for thedetectionof antibodies toB. abortus, B. melitensisandB. suisand for identifying patients suffering relapses. Because of the ease of the procedure, it could be readily adopted for use in clinical laboratories and blood banks.
Fil: Lucero, Nidia E. ANLIS Dr.C.G.Malbrán. Laboratorio de Brucelosis; Argentina.
Fil: Escobar, Gabriela I. ANLIS Dr.C.G.Malbrán. Laboratorio de Brucelosis; Argentina.
Fil: Ayala, Sandra M. ANLIS Dr.C.G.Malbrán. Laboratorio de Brucelosis; Argentina.
Fil: Silva Paulo, Patricia, Comision Nacional de Energia Atomica. Centro Atomico de Ezeiza; Argentina.
Fil: Nielsen, Klaus. Canadian Food Inspection Agency. Animal Diseases Research Institute; Canada.
description Fluorescence polarization immunoassay (FPA) uses molecular rotational properties to measure antibody binding toantigendirectly. Thepotential useof thismethodwas assessed incomparison to a competitive enzyme immunoassay (CELISA) and conventional serological tests for the diagnosis of brucellosis on a total of 587 human sera. Based on 340 sera from asymptomatic blood donors with no evidence of brucellosis, the specificity of the FPAwas97·9% using a cut-off value of 72mP. Sera fromBrucella-infected patients (11Brucellamelitensis,32Brucella abortus,32Brucella suis and oneBrucellasp.) yielded a sensitivity estimate of 96·1%. In tests on 84 sera from suspected brucellosis patients, the FPA detected 80 cases. Of 87 sera from patients with probable infection, 15 were detected by both CELISA and FPA, three by CELISA only and four by FPA only. The discrepancies inbothgroups involvedserawith low, declining titres. TheFPAuses a sampleof 40 l serum, takesabout 5 min tocompleteandhasbeendemonstrated tobeaccurate for thedetectionof antibodies toB. abortus, B. melitensisandB. suisand for identifying patients suffering relapses. Because of the ease of the procedure, it could be readily adopted for use in clinical laboratories and blood banks.
publishDate 2003
dc.date.none.fl_str_mv 2003
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 0022-2615
http://sgc.anlis.gob.ar/handle/123456789/355
http://jmm.sgmjournals.org/content/52/10/883.full.pdf
identifier_str_mv 0022-2615
url http://sgc.anlis.gob.ar/handle/123456789/355
http://jmm.sgmjournals.org/content/52/10/883.full.pdf
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Journal of Medical Microbiology, 2003, 52(10), 883–887.
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
collection Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
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