Serotypes and clonal types of penicillin-susceptible streptococcus pneumoniae causing invasive disease in children in five Latin American countries

• Autores: Zemlicková, Helena; Crisóstomo, M Inês; Brandileone, Maria Cristina; Camou, Teresa; Castañeda, Elizabeth; Corso, Alejandra; Echániz-Avilés, Gabriela; Pásztor, Mónika; Tomasz, Alexander
• Año de publicación: 2005
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Microbial Drug Resistance-Mechanisms Epidemiology and Disease 2005;11(3):195-204.
• Materia: Penicilinas; Streptococcus pneumoniae; América Latina
• Institución: Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"

Fil: Zemlicková, Helena. The Rockefeller University, New York; Estados Unidos.
Fil: Crisóstomo, M Inês. Laboratory of Microbiology, The Rockefeller University, New York; Argentina.
Fil: Brandileone, Maria Cristina. Instituto Adolfo Lutz, São Paulo, Brasil.
Fil: Camou, Teresa. Area of Technology and Health Services Delivery, Unit of Essential Medicines, Vaccines and Health Technology; Estados Unidos.
Fil: Castañeda, Elizabeth. Instituto Nacional de Salud, Bogotá; Colombia.
Fil: Corso, Alejandra. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología; Argentina.
Fil: Echániz-Aviles, Gabriela. Instituto Nacional de Salud Pública, Cuernavaca; Mexico.
Fil: Pásztor, Mónika. Department of Biochemistry, Szent-Györgyi A. Medical University, Szeged; Hungría.
Fil: Tomasz, Alexander. Laboratory of Microbiology, The Rockefeller University, New York; Argentina.
We used multilocus sequencing typing (MLST) to determine the genetic backgrounds of 185 recent penicillin susceptible Streptococcus pneumoniae isolates with serotypes that most frequently cause invasive disease in preschool age children in five Latin American countries-Argentina, Brazil, Colombia, Mexico, and Uruguay. Most of the isolates were associated with pneumonia (90/185), meningitis (74/185), and bacteremia (17/185). The collection of strains included seven serotypes-14, 6B, 5, 1, 23 F-which represent the serotypes of S. pneumoniae most frequently associated with sterile site infections in children. Also included were strains expressing serotypes 7F and 3. Comparison of serotype and multilocus sequence type allowed division of the isolates into two groups: strains expressing serotypes 1, 5, 3, and 7 were represented by a relatively few sequence types while strains expressing serotypes 6B, 14, and 23 F showed great genetic diversity. The genetic diversity of serotypes 14, 6B, and 23 F may be related to the capacity of these serotypes to colonize the nasopharynx of healthy carriers during which opportunities for diversification through genetic exchanges can occur. The findings present an interesting contrast with the results of an earlier study in which over 80% of invasive penicillin- resistant serotype 14 and 23 isolates from the same countries were found to belong to as few as two pandemic clones of S. pneumoniae.