Galectin-1 Prevents Infection and Damage Induced by Trypanosoma cruzi on Cardiac Cells

• Autores: Benatar, Alejandro Francisco; García, Gabriela Andrea; Bua, Jacqueline; Cerliani, Juan P; Postan, Miriam; Tasso, Laura Mónica; Scaglione, Jorge; Stupirski, Juan C; Toscano, Marta A; Rabinovich, Gabriel A; Gómez, Karina A
• Año de publicación: 2015
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: Enfermedad de Chagas; Ratones Noqueados; Miocitos Cardíacos; Interacciones Huésped-Parásitos; Parasitemia
• Institución: Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"

Fil: Benatar, Alejandro Francisco. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI). Laboratorio de Biología Molecular de la Enfermedad de Chagas (LabMECh); Argentina.
Fil: García, Gabriela Andrea. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología Dr. Mario Fatala Chaben; Argentina.
Fil: Bua, Jacqueline. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología Dr. Mario Fatala Chaben; Argentina.
Fil: Cerliani, Juan P. Instituto de Biología y Medicina Experimental (IBYME). Laboratorio de Inmunopatología; Argentina.
Fil: Postam, Miriam. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología Dr. Mario Fatala Chaben; Argentina.
Fil: Tasso, Laura Mónica. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI). Laboratorio de Biología Molecular de la Enfermedad de Chagas (LabMECh); Argentina.
Fil: Scaglione, Jorge. Hospital Pedro de Elizalde. Servicio de Cardiología. Sección Electrofisiología; Buenos Aires, Argentina.
Fil: Stupirski, Juan C. Instituto de Biología y Medicina Experimental (IBYME). Laboratorio de Inmunopatología; Argentina.
Fil: Toscano, Marta A. Instituto de Biología y Medicina Experimental (IBYME). Laboratorio de Inmunopatología; Argentina.
Fil: Rabinovich, Gabriel A. Instituto de Biología y Medicina Experimental (IBYME). Laboratorio de Inmunopatología; Argentina.
Fil: Gómez, Karina A. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI). Laboratorio de Biología Molecular de la Enfermedad de Chagas (LabMECh); Argentina.
Background: Chronic Chagas cardiomyopathy caused by Trypanosoma cruzi is the result of a pathologic process starting during the acute phase of parasite infection. Among different factors, the specific recognition of glycan structures by glycan-binding proteins from the parasite or from the mammalian host cells may play a critical role in the evolution of the infection. Methodology and principal findings: Here we investigated the contribution of galectin-1 (Gal-1), an endogenous glycan-binding protein abundantly expressed in human and mouse heart, to the pathophysiology of T. cruzi infection, particularly in the context of cardiac pathology. We found that exposure of HL-1 cardiac cells to Gal-1 reduced the percentage of infection by two different T. cruzi strains, Tulahuén (TcVI) and Brazil (TcI). In addition, Gal-1 prevented exposure of phosphatidylserine and early events in the apoptotic program by parasite infection on HL-1 cells. These effects were not mediated by direct interaction with the parasite surface, suggesting that Gal-1 may act through binding to host cells. Moreover, we also observed that T. cruzi infection altered the glycophenotype of cardiac cells, reducing binding of exogenous Gal-1 to the cell surface. Consistent with these data, Gal-1 deficient (Lgals1-/-) mice showed increased parasitemia, reduced signs of inflammation in heart and skeletal muscle tissues, and lower survival rates as compared to wild-type (WT) mice in response to intraperitoneal infection with T. cruzi Tulahuén strain. Conclusion/significance: Our results indicate that Gal-1 modulates T. cruzi infection of cardiac cells, highlighting the relevance of galectins and their ligands as regulators of host-parasite interactions.