In vitro infection by different strains of Paracoccidioides brasiliensis

Autores
Canteros, Cristina Elena; Soria, Marcelo; Rivas, María Cristina; Pérez, Celeste Luján; Tous, Mónica; Lee, William; Rodero, Laura; Davel, Graciela Odelsia
Año de publicación
2000
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Canteros, C. E. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.
Fil: Davel, Graciela Odelsia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.
We analyzed the in vitro infection process by P. brasiliensis and the effect of extracellular factor(s) produced on monolayers of mammalian Vero cell lines. The yeast phase of four strains was studied: B339 (avirulent or slightly virulent), U, (intermediate virulence), 93745 and 63265 (both highly virulent). Strains of intermediate and high virulence had higher adherence at first contact (about 16%). Strain B339 had a slower adherence at first contact (8%) than the others during the same period. The production of extracellular proteases, soluble extracellular factor(s) and extracellular antigen gP43 showed no correlation with the in vitro physiopathogenicity of the analyzed strains. We demonstrate that the Vero model presented in this paper is a suitable system to study infection and virulence in vitro. We are currently assessing its usefulness as a tool for the analysis of the interaction between pathogen, host and antifungal agents.
Fuente
Revista Argentina de Microbiología 2000;32(3):116-22
Materia
Animales
Chlorocebus aethiops
Medios de Cultivo
Proteínas Fúngicas
Glicoproteínas
Micología
Oligosacáridos
Paracoccidioides
Especificidad de la Especie
Células Vero
Virulencia
Antígenos Fúngicos
Nivel de accesibilidad
acceso abierto
Condiciones de uso
none
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:123456789/1816
Acceder
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oai_identifier_str oai:sgc.anlis.gob.ar:123456789/1816
network_acronym_str SGCANLIS
repository_id_str a
network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling In vitro infection by different strains of Paracoccidioides brasiliensisCanteros, Cristina ElenaSoria, MarceloRivas, María CristinaPérez, Celeste LujánTous, MónicaLee, WilliamRodero, LauraDavel, Graciela OdelsiaAnimalesChlorocebus aethiopsMedios de CultivoProteínas FúngicasGlicoproteínasMicologíaOligosacáridosParacoccidioidesEspecificidad de la EspecieCélulas VeroVirulenciaAntígenos FúngicosFil: Canteros, C. E. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.Fil: Davel, Graciela Odelsia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.We analyzed the in vitro infection process by P. brasiliensis and the effect of extracellular factor(s) produced on monolayers of mammalian Vero cell lines. The yeast phase of four strains was studied: B339 (avirulent or slightly virulent), U, (intermediate virulence), 93745 and 63265 (both highly virulent). Strains of intermediate and high virulence had higher adherence at first contact (about 16%). Strain B339 had a slower adherence at first contact (8%) than the others during the same period. The production of extracellular proteases, soluble extracellular factor(s) and extracellular antigen gP43 showed no correlation with the in vitro physiopathogenicity of the analyzed strains. We demonstrate that the Vero model presented in this paper is a suitable system to study infection and virulence in vitro. We are currently assessing its usefulness as a tool for the analysis of the interaction between pathogen, host and antifungal agents.2000info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf0325-7541http://sgc.anlis.gob.ar/handle/123456789/1816Revista Argentina de Microbiología 2000;32(3):116-22reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISRevista Argentina de microbiologianoneinfo:eu-repo/semantics/openAccessspa2025-10-16T10:11:42Zoai:sgc.anlis.gob.ar:123456789/1816Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-10-16 10:11:42.449Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv In vitro infection by different strains of Paracoccidioides brasiliensis
title In vitro infection by different strains of Paracoccidioides brasiliensis
spellingShingle In vitro infection by different strains of Paracoccidioides brasiliensis
Canteros, Cristina Elena
Animales
Chlorocebus aethiops
Medios de Cultivo
Proteínas Fúngicas
Glicoproteínas
Micología
Oligosacáridos
Paracoccidioides
Especificidad de la Especie
Células Vero
Virulencia
Antígenos Fúngicos
title_short In vitro infection by different strains of Paracoccidioides brasiliensis
title_full In vitro infection by different strains of Paracoccidioides brasiliensis
title_fullStr In vitro infection by different strains of Paracoccidioides brasiliensis
title_full_unstemmed In vitro infection by different strains of Paracoccidioides brasiliensis
title_sort In vitro infection by different strains of Paracoccidioides brasiliensis
dc.creator.none.fl_str_mv Canteros, Cristina Elena
Soria, Marcelo
Rivas, María Cristina
Pérez, Celeste Luján
Tous, Mónica
Lee, William
Rodero, Laura
Davel, Graciela Odelsia
author Canteros, Cristina Elena
author_facet Canteros, Cristina Elena
Soria, Marcelo
Rivas, María Cristina
Pérez, Celeste Luján
Tous, Mónica
Lee, William
Rodero, Laura
Davel, Graciela Odelsia
author_role author
author2 Soria, Marcelo
Rivas, María Cristina
Pérez, Celeste Luján
Tous, Mónica
Lee, William
Rodero, Laura
Davel, Graciela Odelsia
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Animales
Chlorocebus aethiops
Medios de Cultivo
Proteínas Fúngicas
Glicoproteínas
Micología
Oligosacáridos
Paracoccidioides
Especificidad de la Especie
Células Vero
Virulencia
Antígenos Fúngicos
topic Animales
Chlorocebus aethiops
Medios de Cultivo
Proteínas Fúngicas
Glicoproteínas
Micología
Oligosacáridos
Paracoccidioides
Especificidad de la Especie
Células Vero
Virulencia
Antígenos Fúngicos
dc.description.none.fl_txt_mv Fil: Canteros, C. E. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.
Fil: Davel, Graciela Odelsia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.
We analyzed the in vitro infection process by P. brasiliensis and the effect of extracellular factor(s) produced on monolayers of mammalian Vero cell lines. The yeast phase of four strains was studied: B339 (avirulent or slightly virulent), U, (intermediate virulence), 93745 and 63265 (both highly virulent). Strains of intermediate and high virulence had higher adherence at first contact (about 16%). Strain B339 had a slower adherence at first contact (8%) than the others during the same period. The production of extracellular proteases, soluble extracellular factor(s) and extracellular antigen gP43 showed no correlation with the in vitro physiopathogenicity of the analyzed strains. We demonstrate that the Vero model presented in this paper is a suitable system to study infection and virulence in vitro. We are currently assessing its usefulness as a tool for the analysis of the interaction between pathogen, host and antifungal agents.
description Fil: Canteros, C. E. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.
publishDate 2000
dc.date.none.fl_str_mv 2000
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 0325-7541
http://sgc.anlis.gob.ar/handle/123456789/1816
identifier_str_mv 0325-7541
url http://sgc.anlis.gob.ar/handle/123456789/1816
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv Revista Argentina de microbiologia
dc.rights.none.fl_str_mv none
info:eu-repo/semantics/openAccess
rights_invalid_str_mv none
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Revista Argentina de Microbiología 2000;32(3):116-22
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
collection Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
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score 12.712165

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