Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease

Autores
Allegretti, Yessica Lorena; Bondar, Constanza María; Guzman, Luciana; Cueto Rua, Eduardo; Chopita, Néstor Alfredo; Fuertes, Mercedes; Zwirner, Norberto W.; Chirdo, Fernando Gabriel
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The MICA/B genes (MHC class I chain related genes A and B) encode for non conventional class I HLA molecules which have no role in antigen presentation. MICA/B are up-regulated by different stress conditions such as heat-shock, oxidative stress, neoplasic transformation and viral infection. Particularly, MICA/B are expressed in enterocytes where they can mediate enterocyte apoptosis when recognised by the activating NKG2D receptor present on intraepithelial lymphocytes. This mechanism was suggested to play a major pathogenic role in active celiac disease (CD). Due to the importance of MICA/B in CD pathogenesis we studied their expression in duodenal tissue from CD patients. By immunofluorescence confocal microscopy and flow cytometry we established that MICA/B was mainly intracellularly located in enterocytes. In addition, we identified MICA/B+ T cells in both the intraepithelial and lamina propria compartments. We also found MICA/B+ B cells, plasma cells and some macrophages in the lamina propria. The pattern of MICA/B staining in mucosal tissue in severe enteropathy was similar to that found in in vitro models of cellular stress. In such models, MICA/B were located in stress granules that are associated to the oxidative and ER stress response observed in active CD enteropathy. Our results suggest that expression of MICA/B in the intestinal mucosa of CD patients is linked to disregulation of mucosa homeostasis in which the stress response plays an active role.
Facultad de Ciencias Exactas
Facultad de Ciencias Médicas
Materia
Ciencias Exactas
Ciencias Médicas
Celiac Disease
Cellular Stress
MICA/B genes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/85478

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network_name_str SEDICI (UNLP)
spelling Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac DiseaseAllegretti, Yessica LorenaBondar, Constanza MaríaGuzman, LucianaCueto Rua, EduardoChopita, Néstor AlfredoFuertes, MercedesZwirner, Norberto W.Chirdo, Fernando GabrielCiencias ExactasCiencias MédicasCeliac DiseaseCellular StressMICA/B genesThe MICA/B genes (<i>MHC class I chain related genes A and B</i>) encode for non conventional class I HLA molecules which have no role in antigen presentation. MICA/B are up-regulated by different stress conditions such as heat-shock, oxidative stress, neoplasic transformation and viral infection. Particularly, MICA/B are expressed in enterocytes where they can mediate enterocyte apoptosis when recognised by the activating NKG2D receptor present on intraepithelial lymphocytes. This mechanism was suggested to play a major pathogenic role in active celiac disease (CD). Due to the importance of MICA/B in CD pathogenesis we studied their expression in duodenal tissue from CD patients. By immunofluorescence confocal microscopy and flow cytometry we established that MICA/B was mainly intracellularly located in enterocytes. In addition, we identified MICA/B<SUP>+</SUP> T cells in both the intraepithelial and lamina propria compartments. We also found MICA/B<SUP>+</SUP> B cells, plasma cells and some macrophages in the lamina propria. The pattern of MICA/B staining in mucosal tissue in severe enteropathy was similar to that found in <i>in vitro</i> models of cellular stress. In such models, MICA/B were located in stress granules that are associated to the oxidative and ER stress response observed in active CD enteropathy. Our results suggest that expression of MICA/B in the intestinal mucosa of CD patients is linked to disregulation of mucosa homeostasis in which the stress response plays an active role.Facultad de Ciencias ExactasFacultad de Ciencias Médicas2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/85478enginfo:eu-repo/semantics/altIdentifier/issn/1932-6203info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0073658info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:16:29Zoai:sedici.unlp.edu.ar:10915/85478Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:16:30.184SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease
title Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease
spellingShingle Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease
Allegretti, Yessica Lorena
Ciencias Exactas
Ciencias Médicas
Celiac Disease
Cellular Stress
MICA/B genes
title_short Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease
title_full Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease
title_fullStr Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease
title_full_unstemmed Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease
title_sort Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease
dc.creator.none.fl_str_mv Allegretti, Yessica Lorena
Bondar, Constanza María
Guzman, Luciana
Cueto Rua, Eduardo
Chopita, Néstor Alfredo
Fuertes, Mercedes
Zwirner, Norberto W.
Chirdo, Fernando Gabriel
author Allegretti, Yessica Lorena
author_facet Allegretti, Yessica Lorena
Bondar, Constanza María
Guzman, Luciana
Cueto Rua, Eduardo
Chopita, Néstor Alfredo
Fuertes, Mercedes
Zwirner, Norberto W.
Chirdo, Fernando Gabriel
author_role author
author2 Bondar, Constanza María
Guzman, Luciana
Cueto Rua, Eduardo
Chopita, Néstor Alfredo
Fuertes, Mercedes
Zwirner, Norberto W.
Chirdo, Fernando Gabriel
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Ciencias Médicas
Celiac Disease
Cellular Stress
MICA/B genes
topic Ciencias Exactas
Ciencias Médicas
Celiac Disease
Cellular Stress
MICA/B genes
dc.description.none.fl_txt_mv The MICA/B genes (<i>MHC class I chain related genes A and B</i>) encode for non conventional class I HLA molecules which have no role in antigen presentation. MICA/B are up-regulated by different stress conditions such as heat-shock, oxidative stress, neoplasic transformation and viral infection. Particularly, MICA/B are expressed in enterocytes where they can mediate enterocyte apoptosis when recognised by the activating NKG2D receptor present on intraepithelial lymphocytes. This mechanism was suggested to play a major pathogenic role in active celiac disease (CD). Due to the importance of MICA/B in CD pathogenesis we studied their expression in duodenal tissue from CD patients. By immunofluorescence confocal microscopy and flow cytometry we established that MICA/B was mainly intracellularly located in enterocytes. In addition, we identified MICA/B<SUP>+</SUP> T cells in both the intraepithelial and lamina propria compartments. We also found MICA/B<SUP>+</SUP> B cells, plasma cells and some macrophages in the lamina propria. The pattern of MICA/B staining in mucosal tissue in severe enteropathy was similar to that found in <i>in vitro</i> models of cellular stress. In such models, MICA/B were located in stress granules that are associated to the oxidative and ER stress response observed in active CD enteropathy. Our results suggest that expression of MICA/B in the intestinal mucosa of CD patients is linked to disregulation of mucosa homeostasis in which the stress response plays an active role.
Facultad de Ciencias Exactas
Facultad de Ciencias Médicas
description The MICA/B genes (<i>MHC class I chain related genes A and B</i>) encode for non conventional class I HLA molecules which have no role in antigen presentation. MICA/B are up-regulated by different stress conditions such as heat-shock, oxidative stress, neoplasic transformation and viral infection. Particularly, MICA/B are expressed in enterocytes where they can mediate enterocyte apoptosis when recognised by the activating NKG2D receptor present on intraepithelial lymphocytes. This mechanism was suggested to play a major pathogenic role in active celiac disease (CD). Due to the importance of MICA/B in CD pathogenesis we studied their expression in duodenal tissue from CD patients. By immunofluorescence confocal microscopy and flow cytometry we established that MICA/B was mainly intracellularly located in enterocytes. In addition, we identified MICA/B<SUP>+</SUP> T cells in both the intraepithelial and lamina propria compartments. We also found MICA/B<SUP>+</SUP> B cells, plasma cells and some macrophages in the lamina propria. The pattern of MICA/B staining in mucosal tissue in severe enteropathy was similar to that found in <i>in vitro</i> models of cellular stress. In such models, MICA/B were located in stress granules that are associated to the oxidative and ER stress response observed in active CD enteropathy. Our results suggest that expression of MICA/B in the intestinal mucosa of CD patients is linked to disregulation of mucosa homeostasis in which the stress response plays an active role.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/85478
url http://sedici.unlp.edu.ar/handle/10915/85478
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1932-6203
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0073658
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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