Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease
- Autores
- Allegretti, Yessica Lorena; Bondar, Constanza María; Guzman, Luciana; Cueto Rua, Eduardo; Chopita, Néstor Alfredo; Fuertes, Mercedes; Zwirner, Norberto W.; Chirdo, Fernando Gabriel
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The MICA/B genes (MHC class I chain related genes A and B) encode for non conventional class I HLA molecules which have no role in antigen presentation. MICA/B are up-regulated by different stress conditions such as heat-shock, oxidative stress, neoplasic transformation and viral infection. Particularly, MICA/B are expressed in enterocytes where they can mediate enterocyte apoptosis when recognised by the activating NKG2D receptor present on intraepithelial lymphocytes. This mechanism was suggested to play a major pathogenic role in active celiac disease (CD). Due to the importance of MICA/B in CD pathogenesis we studied their expression in duodenal tissue from CD patients. By immunofluorescence confocal microscopy and flow cytometry we established that MICA/B was mainly intracellularly located in enterocytes. In addition, we identified MICA/B+ T cells in both the intraepithelial and lamina propria compartments. We also found MICA/B+ B cells, plasma cells and some macrophages in the lamina propria. The pattern of MICA/B staining in mucosal tissue in severe enteropathy was similar to that found in in vitro models of cellular stress. In such models, MICA/B were located in stress granules that are associated to the oxidative and ER stress response observed in active CD enteropathy. Our results suggest that expression of MICA/B in the intestinal mucosa of CD patients is linked to disregulation of mucosa homeostasis in which the stress response plays an active role.
Facultad de Ciencias Exactas
Facultad de Ciencias Médicas - Materia
-
Ciencias Exactas
Ciencias Médicas
Celiac Disease
Cellular Stress
MICA/B genes - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/85478
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Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac DiseaseAllegretti, Yessica LorenaBondar, Constanza MaríaGuzman, LucianaCueto Rua, EduardoChopita, Néstor AlfredoFuertes, MercedesZwirner, Norberto W.Chirdo, Fernando GabrielCiencias ExactasCiencias MédicasCeliac DiseaseCellular StressMICA/B genesThe MICA/B genes (<i>MHC class I chain related genes A and B</i>) encode for non conventional class I HLA molecules which have no role in antigen presentation. MICA/B are up-regulated by different stress conditions such as heat-shock, oxidative stress, neoplasic transformation and viral infection. Particularly, MICA/B are expressed in enterocytes where they can mediate enterocyte apoptosis when recognised by the activating NKG2D receptor present on intraepithelial lymphocytes. This mechanism was suggested to play a major pathogenic role in active celiac disease (CD). Due to the importance of MICA/B in CD pathogenesis we studied their expression in duodenal tissue from CD patients. By immunofluorescence confocal microscopy and flow cytometry we established that MICA/B was mainly intracellularly located in enterocytes. In addition, we identified MICA/B<SUP>+</SUP> T cells in both the intraepithelial and lamina propria compartments. We also found MICA/B<SUP>+</SUP> B cells, plasma cells and some macrophages in the lamina propria. The pattern of MICA/B staining in mucosal tissue in severe enteropathy was similar to that found in <i>in vitro</i> models of cellular stress. In such models, MICA/B were located in stress granules that are associated to the oxidative and ER stress response observed in active CD enteropathy. Our results suggest that expression of MICA/B in the intestinal mucosa of CD patients is linked to disregulation of mucosa homeostasis in which the stress response plays an active role.Facultad de Ciencias ExactasFacultad de Ciencias Médicas2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/85478enginfo:eu-repo/semantics/altIdentifier/issn/1932-6203info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0073658info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:16:29Zoai:sedici.unlp.edu.ar:10915/85478Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:16:30.184SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease |
title |
Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease |
spellingShingle |
Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease Allegretti, Yessica Lorena Ciencias Exactas Ciencias Médicas Celiac Disease Cellular Stress MICA/B genes |
title_short |
Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease |
title_full |
Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease |
title_fullStr |
Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease |
title_full_unstemmed |
Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease |
title_sort |
Broad MICA/B Expression in the Small Bowel Mucosa: A Link between Cellular Stress and Celiac Disease |
dc.creator.none.fl_str_mv |
Allegretti, Yessica Lorena Bondar, Constanza María Guzman, Luciana Cueto Rua, Eduardo Chopita, Néstor Alfredo Fuertes, Mercedes Zwirner, Norberto W. Chirdo, Fernando Gabriel |
author |
Allegretti, Yessica Lorena |
author_facet |
Allegretti, Yessica Lorena Bondar, Constanza María Guzman, Luciana Cueto Rua, Eduardo Chopita, Néstor Alfredo Fuertes, Mercedes Zwirner, Norberto W. Chirdo, Fernando Gabriel |
author_role |
author |
author2 |
Bondar, Constanza María Guzman, Luciana Cueto Rua, Eduardo Chopita, Néstor Alfredo Fuertes, Mercedes Zwirner, Norberto W. Chirdo, Fernando Gabriel |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
Ciencias Exactas Ciencias Médicas Celiac Disease Cellular Stress MICA/B genes |
topic |
Ciencias Exactas Ciencias Médicas Celiac Disease Cellular Stress MICA/B genes |
dc.description.none.fl_txt_mv |
The MICA/B genes (<i>MHC class I chain related genes A and B</i>) encode for non conventional class I HLA molecules which have no role in antigen presentation. MICA/B are up-regulated by different stress conditions such as heat-shock, oxidative stress, neoplasic transformation and viral infection. Particularly, MICA/B are expressed in enterocytes where they can mediate enterocyte apoptosis when recognised by the activating NKG2D receptor present on intraepithelial lymphocytes. This mechanism was suggested to play a major pathogenic role in active celiac disease (CD). Due to the importance of MICA/B in CD pathogenesis we studied their expression in duodenal tissue from CD patients. By immunofluorescence confocal microscopy and flow cytometry we established that MICA/B was mainly intracellularly located in enterocytes. In addition, we identified MICA/B<SUP>+</SUP> T cells in both the intraepithelial and lamina propria compartments. We also found MICA/B<SUP>+</SUP> B cells, plasma cells and some macrophages in the lamina propria. The pattern of MICA/B staining in mucosal tissue in severe enteropathy was similar to that found in <i>in vitro</i> models of cellular stress. In such models, MICA/B were located in stress granules that are associated to the oxidative and ER stress response observed in active CD enteropathy. Our results suggest that expression of MICA/B in the intestinal mucosa of CD patients is linked to disregulation of mucosa homeostasis in which the stress response plays an active role. Facultad de Ciencias Exactas Facultad de Ciencias Médicas |
description |
The MICA/B genes (<i>MHC class I chain related genes A and B</i>) encode for non conventional class I HLA molecules which have no role in antigen presentation. MICA/B are up-regulated by different stress conditions such as heat-shock, oxidative stress, neoplasic transformation and viral infection. Particularly, MICA/B are expressed in enterocytes where they can mediate enterocyte apoptosis when recognised by the activating NKG2D receptor present on intraepithelial lymphocytes. This mechanism was suggested to play a major pathogenic role in active celiac disease (CD). Due to the importance of MICA/B in CD pathogenesis we studied their expression in duodenal tissue from CD patients. By immunofluorescence confocal microscopy and flow cytometry we established that MICA/B was mainly intracellularly located in enterocytes. In addition, we identified MICA/B<SUP>+</SUP> T cells in both the intraepithelial and lamina propria compartments. We also found MICA/B<SUP>+</SUP> B cells, plasma cells and some macrophages in the lamina propria. The pattern of MICA/B staining in mucosal tissue in severe enteropathy was similar to that found in <i>in vitro</i> models of cellular stress. In such models, MICA/B were located in stress granules that are associated to the oxidative and ER stress response observed in active CD enteropathy. Our results suggest that expression of MICA/B in the intestinal mucosa of CD patients is linked to disregulation of mucosa homeostasis in which the stress response plays an active role. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
status_str |
publishedVersion |
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http://sedici.unlp.edu.ar/handle/10915/85478 |
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http://sedici.unlp.edu.ar/handle/10915/85478 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
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info:eu-repo/semantics/altIdentifier/issn/1932-6203 info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0073658 |
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info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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openAccess |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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