An alternative method in distinguishing cattle transferrin phenotypes
- Autores
- Quinteros, Indalecio Rodolfo; Miller, Wilmer J.
- Año de publicación
- 1968
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- A modification of the method described by Kristjansson (1963) allows easier distinction of the components and position of every major cattle transferrin phenotype. The modification is based on increasing the percentage of starch (15%) and reducing the pH of the gel buffer to 6.8. In all the experiments, when a voltage of 350 was applied, a tray of ice was placed over the starch gel for the remainder of the electrophoresis. Different cattle transferrin phenotypes from our modified electrophoresis method are composed as follows: Type A, 4 bands; D1, 4 bands; D1D2, 4 bands; D2, 4 bands; E, 4 bands; AD1, 6 bands; AD2, 6 bands; AE, 8 bands and sometimes 9 bands; D1E, 6 bands and sometimes 7 bands; and D2E, 6 bands. The position of the fourth D band is distinctly different in D1 vs. D2 types.
Universidad Nacional de La Plata - Materia
-
Veterinaria
Química
cattle transferrin phenotype
starch
gel buffer
electrophoresis - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/142057
Ver los metadatos del registro completo
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spelling |
An alternative method in distinguishing cattle transferrin phenotypesQuinteros, Indalecio RodolfoMiller, Wilmer J.VeterinariaQuímicacattle transferrin phenotypestarchgel bufferelectrophoresisA modification of the method described by Kristjansson (1963) allows easier distinction of the components and position of every major cattle transferrin phenotype. The modification is based on increasing the percentage of starch (15%) and reducing the pH of the gel buffer to 6.8. In all the experiments, when a voltage of 350 was applied, a tray of ice was placed over the starch gel for the remainder of the electrophoresis. Different cattle transferrin phenotypes from our modified electrophoresis method are composed as follows: Type A, 4 bands; D1, 4 bands; D1D2, 4 bands; D2, 4 bands; E, 4 bands; AD1, 6 bands; AD2, 6 bands; AE, 8 bands and sometimes 9 bands; D1E, 6 bands and sometimes 7 bands; and D2E, 6 bands. The position of the fourth D band is distinctly different in D1 vs. D2 types.Universidad Nacional de La Plata1968info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf213-218http://sedici.unlp.edu.ar/handle/10915/142057enginfo:eu-repo/semantics/altIdentifier/issn/0006-2928info:eu-repo/semantics/altIdentifier/issn/1573-4927info:eu-repo/semantics/altIdentifier/doi/10.1007/bf01474760info:eu-repo/semantics/altIdentifier/pmid/5715185info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:11Zoai:sedici.unlp.edu.ar:10915/142057Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:12.256SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
An alternative method in distinguishing cattle transferrin phenotypes |
title |
An alternative method in distinguishing cattle transferrin phenotypes |
spellingShingle |
An alternative method in distinguishing cattle transferrin phenotypes Quinteros, Indalecio Rodolfo Veterinaria Química cattle transferrin phenotype starch gel buffer electrophoresis |
title_short |
An alternative method in distinguishing cattle transferrin phenotypes |
title_full |
An alternative method in distinguishing cattle transferrin phenotypes |
title_fullStr |
An alternative method in distinguishing cattle transferrin phenotypes |
title_full_unstemmed |
An alternative method in distinguishing cattle transferrin phenotypes |
title_sort |
An alternative method in distinguishing cattle transferrin phenotypes |
dc.creator.none.fl_str_mv |
Quinteros, Indalecio Rodolfo Miller, Wilmer J. |
author |
Quinteros, Indalecio Rodolfo |
author_facet |
Quinteros, Indalecio Rodolfo Miller, Wilmer J. |
author_role |
author |
author2 |
Miller, Wilmer J. |
author2_role |
author |
dc.subject.none.fl_str_mv |
Veterinaria Química cattle transferrin phenotype starch gel buffer electrophoresis |
topic |
Veterinaria Química cattle transferrin phenotype starch gel buffer electrophoresis |
dc.description.none.fl_txt_mv |
A modification of the method described by Kristjansson (1963) allows easier distinction of the components and position of every major cattle transferrin phenotype. The modification is based on increasing the percentage of starch (15%) and reducing the pH of the gel buffer to 6.8. In all the experiments, when a voltage of 350 was applied, a tray of ice was placed over the starch gel for the remainder of the electrophoresis. Different cattle transferrin phenotypes from our modified electrophoresis method are composed as follows: Type A, 4 bands; D1, 4 bands; D1D2, 4 bands; D2, 4 bands; E, 4 bands; AD1, 6 bands; AD2, 6 bands; AE, 8 bands and sometimes 9 bands; D1E, 6 bands and sometimes 7 bands; and D2E, 6 bands. The position of the fourth D band is distinctly different in D1 vs. D2 types. Universidad Nacional de La Plata |
description |
A modification of the method described by Kristjansson (1963) allows easier distinction of the components and position of every major cattle transferrin phenotype. The modification is based on increasing the percentage of starch (15%) and reducing the pH of the gel buffer to 6.8. In all the experiments, when a voltage of 350 was applied, a tray of ice was placed over the starch gel for the remainder of the electrophoresis. Different cattle transferrin phenotypes from our modified electrophoresis method are composed as follows: Type A, 4 bands; D1, 4 bands; D1D2, 4 bands; D2, 4 bands; E, 4 bands; AD1, 6 bands; AD2, 6 bands; AE, 8 bands and sometimes 9 bands; D1E, 6 bands and sometimes 7 bands; and D2E, 6 bands. The position of the fourth D band is distinctly different in D1 vs. D2 types. |
publishDate |
1968 |
dc.date.none.fl_str_mv |
1968 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/142057 |
url |
http://sedici.unlp.edu.ar/handle/10915/142057 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/issn/0006-2928 info:eu-repo/semantics/altIdentifier/issn/1573-4927 info:eu-repo/semantics/altIdentifier/doi/10.1007/bf01474760 info:eu-repo/semantics/altIdentifier/pmid/5715185 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
dc.format.none.fl_str_mv |
application/pdf 213-218 |
dc.source.none.fl_str_mv |
reponame:SEDICI (UNLP) instname:Universidad Nacional de La Plata instacron:UNLP |
reponame_str |
SEDICI (UNLP) |
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SEDICI (UNLP) |
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Universidad Nacional de La Plata |
instacron_str |
UNLP |
institution |
UNLP |
repository.name.fl_str_mv |
SEDICI (UNLP) - Universidad Nacional de La Plata |
repository.mail.fl_str_mv |
alira@sedici.unlp.edu.ar |
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1844616201523691520 |
score |
13.070432 |