An alternative method in distinguishing cattle transferrin phenotypes

Autores
Quinteros, Indalecio Rodolfo; Miller, Wilmer J.
Año de publicación
1968
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A modification of the method described by Kristjansson (1963) allows easier distinction of the components and position of every major cattle transferrin phenotype. The modification is based on increasing the percentage of starch (15%) and reducing the pH of the gel buffer to 6.8. In all the experiments, when a voltage of 350 was applied, a tray of ice was placed over the starch gel for the remainder of the electrophoresis. Different cattle transferrin phenotypes from our modified electrophoresis method are composed as follows: Type A, 4 bands; D1, 4 bands; D1D2, 4 bands; D2, 4 bands; E, 4 bands; AD1, 6 bands; AD2, 6 bands; AE, 8 bands and sometimes 9 bands; D1E, 6 bands and sometimes 7 bands; and D2E, 6 bands. The position of the fourth D band is distinctly different in D1 vs. D2 types.
Universidad Nacional de La Plata
Materia
Veterinaria
Química
cattle transferrin phenotype
starch
gel buffer
electrophoresis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/142057
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/142057
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling An alternative method in distinguishing cattle transferrin phenotypesQuinteros, Indalecio RodolfoMiller, Wilmer J.VeterinariaQuímicacattle transferrin phenotypestarchgel bufferelectrophoresisA modification of the method described by Kristjansson (1963) allows easier distinction of the components and position of every major cattle transferrin phenotype. The modification is based on increasing the percentage of starch (15%) and reducing the pH of the gel buffer to 6.8. In all the experiments, when a voltage of 350 was applied, a tray of ice was placed over the starch gel for the remainder of the electrophoresis. Different cattle transferrin phenotypes from our modified electrophoresis method are composed as follows: Type A, 4 bands; D1, 4 bands; D1D2, 4 bands; D2, 4 bands; E, 4 bands; AD1, 6 bands; AD2, 6 bands; AE, 8 bands and sometimes 9 bands; D1E, 6 bands and sometimes 7 bands; and D2E, 6 bands. The position of the fourth D band is distinctly different in D1 vs. D2 types.Universidad Nacional de La Plata1968info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf213-218http://sedici.unlp.edu.ar/handle/10915/142057enginfo:eu-repo/semantics/altIdentifier/issn/0006-2928info:eu-repo/semantics/altIdentifier/issn/1573-4927info:eu-repo/semantics/altIdentifier/doi/10.1007/bf01474760info:eu-repo/semantics/altIdentifier/pmid/5715185info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-22T17:13:06Zoai:sedici.unlp.edu.ar:10915/142057Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-22 17:13:07.17SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv An alternative method in distinguishing cattle transferrin phenotypes
title An alternative method in distinguishing cattle transferrin phenotypes
spellingShingle An alternative method in distinguishing cattle transferrin phenotypes
Quinteros, Indalecio Rodolfo
Veterinaria
Química
cattle transferrin phenotype
starch
gel buffer
electrophoresis
title_short An alternative method in distinguishing cattle transferrin phenotypes
title_full An alternative method in distinguishing cattle transferrin phenotypes
title_fullStr An alternative method in distinguishing cattle transferrin phenotypes
title_full_unstemmed An alternative method in distinguishing cattle transferrin phenotypes
title_sort An alternative method in distinguishing cattle transferrin phenotypes
dc.creator.none.fl_str_mv Quinteros, Indalecio Rodolfo
Miller, Wilmer J.
author Quinteros, Indalecio Rodolfo
author_facet Quinteros, Indalecio Rodolfo
Miller, Wilmer J.
author_role author
author2 Miller, Wilmer J.
author2_role author
dc.subject.none.fl_str_mv Veterinaria
Química
cattle transferrin phenotype
starch
gel buffer
electrophoresis
topic Veterinaria
Química
cattle transferrin phenotype
starch
gel buffer
electrophoresis
dc.description.none.fl_txt_mv A modification of the method described by Kristjansson (1963) allows easier distinction of the components and position of every major cattle transferrin phenotype. The modification is based on increasing the percentage of starch (15%) and reducing the pH of the gel buffer to 6.8. In all the experiments, when a voltage of 350 was applied, a tray of ice was placed over the starch gel for the remainder of the electrophoresis. Different cattle transferrin phenotypes from our modified electrophoresis method are composed as follows: Type A, 4 bands; D1, 4 bands; D1D2, 4 bands; D2, 4 bands; E, 4 bands; AD1, 6 bands; AD2, 6 bands; AE, 8 bands and sometimes 9 bands; D1E, 6 bands and sometimes 7 bands; and D2E, 6 bands. The position of the fourth D band is distinctly different in D1 vs. D2 types.
Universidad Nacional de La Plata
description A modification of the method described by Kristjansson (1963) allows easier distinction of the components and position of every major cattle transferrin phenotype. The modification is based on increasing the percentage of starch (15%) and reducing the pH of the gel buffer to 6.8. In all the experiments, when a voltage of 350 was applied, a tray of ice was placed over the starch gel for the remainder of the electrophoresis. Different cattle transferrin phenotypes from our modified electrophoresis method are composed as follows: Type A, 4 bands; D1, 4 bands; D1D2, 4 bands; D2, 4 bands; E, 4 bands; AD1, 6 bands; AD2, 6 bands; AE, 8 bands and sometimes 9 bands; D1E, 6 bands and sometimes 7 bands; and D2E, 6 bands. The position of the fourth D band is distinctly different in D1 vs. D2 types.
publishDate 1968
dc.date.none.fl_str_mv 1968
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/142057
url http://sedici.unlp.edu.ar/handle/10915/142057
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0006-2928
info:eu-repo/semantics/altIdentifier/issn/1573-4927
info:eu-repo/semantics/altIdentifier/doi/10.1007/bf01474760
info:eu-repo/semantics/altIdentifier/pmid/5715185
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
213-218
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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