Lack of Galactose or Galacturonic Acid in <i>Bradyrhizobium japonicum</i> USDA 110 Exopolysaccharide Leads to Different Symbiotic Responses in Soybean

Autores
Quelas, Juan Ignacio; Mongiardini, Elías Javier; Casabuono, Adriana C.; López García, Silvina Laura; Althabegoiti, María Julia; Covelli, Julieta Mariana; Pérez Giménez, Julieta; Couto, Alicia S.; Lodeiro, Aníbal Roberto
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Exopolysaccharide (EPS) and lipopolysaccharide (LPS) from Bradyrhizobium japonicum are important for infection and nodulation of soybean (Glycine max), although their roles are not completely understood. To better understand this, we constructed mutants in B. japonicum USDA 110 impaired in galactose or galacturonic acid incorporation into the EPS without affecting the LPS. The derivative LP 3010 had a deletion of lspL-ugdH and produced EPS without galacturonic acid whereas LP 3013, with an insertion in exoB, produced EPS without galactose. In addition, the strain LP 3017, with both mutations, had EPS devoid of both galactosides. The missing galactosides were not replaced by other sugars. The defects in EPS had different consequences. LP 3010 formed biofilms and nodulated but was defective in competitiveness for nodulation; and, inside nodules, the peribacteroid membranes tended to fuse, leading to the merging of symbiosomes. Meanwhile, LP 3013 and LP 3017 were unable to form biofilms and produced empty pseudonodules but exoB suppressor mutants were obtained when LP 3013 plant inoculation was supplemented with wild-type EPS. Similar phenotypes were observed with all these mutants in G. soja. Therefore, the lack of each galactoside in the EPS has a different functional effect on the B. japonicum-soybean symbiosis.
Facultad de Ciencias Exactas
Instituto de Biotecnologia y Biologia Molecular
Materia
Ciencias Exactas
Biología
Química
exopolysaccharide
lipopolysaccharide
Bradyrhizobium japonicum
mutants
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/123573
Acceder
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repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Lack of Galactose or Galacturonic Acid in <i>Bradyrhizobium japonicum</i> USDA 110 Exopolysaccharide Leads to Different Symbiotic Responses in SoybeanQuelas, Juan IgnacioMongiardini, Elías JavierCasabuono, Adriana C.López García, Silvina LauraAlthabegoiti, María JuliaCovelli, Julieta MarianaPérez Giménez, JulietaCouto, Alicia S.Lodeiro, Aníbal RobertoCiencias ExactasBiologíaQuímicaexopolysaccharidelipopolysaccharideBradyrhizobium japonicummutantsExopolysaccharide (EPS) and lipopolysaccharide (LPS) from <i>Bradyrhizobium japonicum</i> are important for infection and nodulation of soybean (<i>Glycine max</i>), although their roles are not completely understood. To better understand this, we constructed mutants in <i>B. japonicum</i> USDA 110 impaired in galactose or galacturonic acid incorporation into the EPS without affecting the LPS. The derivative LP 3010 had a deletion of <i>lspL-ugdH</i> and produced EPS without galacturonic acid whereas LP 3013, with an insertion in <i>exoB</i>, produced EPS without galactose. In addition, the strain LP 3017, with both mutations, had EPS devoid of both galactosides. The missing galactosides were not replaced by other sugars. The defects in EPS had different consequences. LP 3010 formed biofilms and nodulated but was defective in competitiveness for nodulation; and, inside nodules, the peribacteroid membranes tended to fuse, leading to the merging of symbiosomes. Meanwhile, LP 3013 and LP 3017 were unable to form biofilms and produced empty pseudonodules but <i>exoB</i> suppressor mutants were obtained when LP 3013 plant inoculation was supplemented with wild-type EPS. Similar phenotypes were observed with all these mutants in <i>G. soja</i>. Therefore, the lack of each galactoside in the EPS has a different functional effect on the <i>B. japonicum</i>-soybean symbiosis.Facultad de Ciencias ExactasInstituto de Biotecnologia y Biologia Molecular2010info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1592-1604http://sedici.unlp.edu.ar/handle/10915/123573enginfo:eu-repo/semantics/altIdentifier/issn/0894-0282info:eu-repo/semantics/altIdentifier/issn/1943-7706info:eu-repo/semantics/altIdentifier/pmid/20636103info:eu-repo/semantics/altIdentifier/doi/10.1094/mpmi-05-10-0122info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:01:34Zoai:sedici.unlp.edu.ar:10915/123573Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:01:35.202SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Lack of Galactose or Galacturonic Acid in <i>Bradyrhizobium japonicum</i> USDA 110 Exopolysaccharide Leads to Different Symbiotic Responses in Soybean
title Lack of Galactose or Galacturonic Acid in <i>Bradyrhizobium japonicum</i> USDA 110 Exopolysaccharide Leads to Different Symbiotic Responses in Soybean
spellingShingle Lack of Galactose or Galacturonic Acid in <i>Bradyrhizobium japonicum</i> USDA 110 Exopolysaccharide Leads to Different Symbiotic Responses in Soybean
Quelas, Juan Ignacio
Ciencias Exactas
Biología
Química
exopolysaccharide
lipopolysaccharide
Bradyrhizobium japonicum
mutants
title_short Lack of Galactose or Galacturonic Acid in <i>Bradyrhizobium japonicum</i> USDA 110 Exopolysaccharide Leads to Different Symbiotic Responses in Soybean
title_full Lack of Galactose or Galacturonic Acid in <i>Bradyrhizobium japonicum</i> USDA 110 Exopolysaccharide Leads to Different Symbiotic Responses in Soybean
title_fullStr Lack of Galactose or Galacturonic Acid in <i>Bradyrhizobium japonicum</i> USDA 110 Exopolysaccharide Leads to Different Symbiotic Responses in Soybean
title_full_unstemmed Lack of Galactose or Galacturonic Acid in <i>Bradyrhizobium japonicum</i> USDA 110 Exopolysaccharide Leads to Different Symbiotic Responses in Soybean
title_sort Lack of Galactose or Galacturonic Acid in <i>Bradyrhizobium japonicum</i> USDA 110 Exopolysaccharide Leads to Different Symbiotic Responses in Soybean
dc.creator.none.fl_str_mv Quelas, Juan Ignacio
Mongiardini, Elías Javier
Casabuono, Adriana C.
López García, Silvina Laura
Althabegoiti, María Julia
Covelli, Julieta Mariana
Pérez Giménez, Julieta
Couto, Alicia S.
Lodeiro, Aníbal Roberto
author Quelas, Juan Ignacio
author_facet Quelas, Juan Ignacio
Mongiardini, Elías Javier
Casabuono, Adriana C.
López García, Silvina Laura
Althabegoiti, María Julia
Covelli, Julieta Mariana
Pérez Giménez, Julieta
Couto, Alicia S.
Lodeiro, Aníbal Roberto
author_role author
author2 Mongiardini, Elías Javier
Casabuono, Adriana C.
López García, Silvina Laura
Althabegoiti, María Julia
Covelli, Julieta Mariana
Pérez Giménez, Julieta
Couto, Alicia S.
Lodeiro, Aníbal Roberto
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Biología
Química
exopolysaccharide
lipopolysaccharide
Bradyrhizobium japonicum
mutants
topic Ciencias Exactas
Biología
Química
exopolysaccharide
lipopolysaccharide
Bradyrhizobium japonicum
mutants
dc.description.none.fl_txt_mv Exopolysaccharide (EPS) and lipopolysaccharide (LPS) from <i>Bradyrhizobium japonicum</i> are important for infection and nodulation of soybean (<i>Glycine max</i>), although their roles are not completely understood. To better understand this, we constructed mutants in <i>B. japonicum</i> USDA 110 impaired in galactose or galacturonic acid incorporation into the EPS without affecting the LPS. The derivative LP 3010 had a deletion of <i>lspL-ugdH</i> and produced EPS without galacturonic acid whereas LP 3013, with an insertion in <i>exoB</i>, produced EPS without galactose. In addition, the strain LP 3017, with both mutations, had EPS devoid of both galactosides. The missing galactosides were not replaced by other sugars. The defects in EPS had different consequences. LP 3010 formed biofilms and nodulated but was defective in competitiveness for nodulation; and, inside nodules, the peribacteroid membranes tended to fuse, leading to the merging of symbiosomes. Meanwhile, LP 3013 and LP 3017 were unable to form biofilms and produced empty pseudonodules but <i>exoB</i> suppressor mutants were obtained when LP 3013 plant inoculation was supplemented with wild-type EPS. Similar phenotypes were observed with all these mutants in <i>G. soja</i>. Therefore, the lack of each galactoside in the EPS has a different functional effect on the <i>B. japonicum</i>-soybean symbiosis.
Facultad de Ciencias Exactas
Instituto de Biotecnologia y Biologia Molecular
description Exopolysaccharide (EPS) and lipopolysaccharide (LPS) from <i>Bradyrhizobium japonicum</i> are important for infection and nodulation of soybean (<i>Glycine max</i>), although their roles are not completely understood. To better understand this, we constructed mutants in <i>B. japonicum</i> USDA 110 impaired in galactose or galacturonic acid incorporation into the EPS without affecting the LPS. The derivative LP 3010 had a deletion of <i>lspL-ugdH</i> and produced EPS without galacturonic acid whereas LP 3013, with an insertion in <i>exoB</i>, produced EPS without galactose. In addition, the strain LP 3017, with both mutations, had EPS devoid of both galactosides. The missing galactosides were not replaced by other sugars. The defects in EPS had different consequences. LP 3010 formed biofilms and nodulated but was defective in competitiveness for nodulation; and, inside nodules, the peribacteroid membranes tended to fuse, leading to the merging of symbiosomes. Meanwhile, LP 3013 and LP 3017 were unable to form biofilms and produced empty pseudonodules but <i>exoB</i> suppressor mutants were obtained when LP 3013 plant inoculation was supplemented with wild-type EPS. Similar phenotypes were observed with all these mutants in <i>G. soja</i>. Therefore, the lack of each galactoside in the EPS has a different functional effect on the <i>B. japonicum</i>-soybean symbiosis.
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/123573
url http://sedici.unlp.edu.ar/handle/10915/123573
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/issn/1943-7706
info:eu-repo/semantics/altIdentifier/pmid/20636103
info:eu-repo/semantics/altIdentifier/doi/10.1094/mpmi-05-10-0122
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
1592-1604
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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