Analysis of the source of heterogeneity in the osmotic response of plant membrane vesicles
- Autores
- Alleva, Karina; Chara, Osvaldo; Sutka, Moira; Amodeo, Gabriela
- Año de publicación
- 2008
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Plasma membrane vesicles have been widely employed to understand the biophysics of water movements, especially when active aquaporins are present. In general, water permeability coefficients in these preparations outcome from the analysis of the osmotic response of the vesicles by means of light scattering. As from now, this is possible by following a theoretical approach that assumes that scattered light follows a single exponential function and that this behavior is the consequence of vesicle volume changes due to an osmotic challenge. However, some experimental data do not necessarily fit to single exponentials but to double ones. It is argued that the observed double exponential behavior has two possible causes: different vesicle population in terms of permeability or in terms of size distribution. As classical models cannot identify this source of heterogeneity, a mathematical modeling approach was developed based on phenomenological equations of water transport. In the three comparative models presented here, it was assumed that water moves according to an osmotic mechanism across the vesicles, and there is no solute movement across them. Interestingly, when tested in a well described plasma membrane vesicle preparation, the application of these models indicates that the source of heterogeneity in the osmotic response is vesicles having different permeability, clearly discarding the variable size effect. In conclusion, the mathematical approach presented here allows to identify the source of heterogeneity; this information being of particular interest, especially when studying gating mechanisms triggered in water channel activity.
Instituto de Física de Líquidos y Sistemas Biológicos - Materia
-
Ciencias Exactas
Biología
Aquaporins
Stopped flow
Osmotic permeability
Mathematical modeling
Simulations
Water transport - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/145882
Ver los metadatos del registro completo
id |
SEDICI_f4e27f6fc8cc1a3b89c2992eb65afb1d |
---|---|
oai_identifier_str |
oai:sedici.unlp.edu.ar:10915/145882 |
network_acronym_str |
SEDICI |
repository_id_str |
1329 |
network_name_str |
SEDICI (UNLP) |
spelling |
Analysis of the source of heterogeneity in the osmotic response of plant membrane vesiclesAlleva, KarinaChara, OsvaldoSutka, MoiraAmodeo, GabrielaCiencias ExactasBiologíaAquaporinsStopped flowOsmotic permeabilityMathematical modelingSimulationsWater transportPlasma membrane vesicles have been widely employed to understand the biophysics of water movements, especially when active aquaporins are present. In general, water permeability coefficients in these preparations outcome from the analysis of the osmotic response of the vesicles by means of light scattering. As from now, this is possible by following a theoretical approach that assumes that scattered light follows a single exponential function and that this behavior is the consequence of vesicle volume changes due to an osmotic challenge. However, some experimental data do not necessarily fit to single exponentials but to double ones. It is argued that the observed double exponential behavior has two possible causes: different vesicle population in terms of permeability or in terms of size distribution. As classical models cannot identify this source of heterogeneity, a mathematical modeling approach was developed based on phenomenological equations of water transport. In the three comparative models presented here, it was assumed that water moves according to an osmotic mechanism across the vesicles, and there is no solute movement across them. Interestingly, when tested in a well described plasma membrane vesicle preparation, the application of these models indicates that the source of heterogeneity in the osmotic response is vesicles having different permeability, clearly discarding the variable size effect. In conclusion, the mathematical approach presented here allows to identify the source of heterogeneity; this information being of particular interest, especially when studying gating mechanisms triggered in water channel activity.Instituto de Física de Líquidos y Sistemas Biológicos2008-09-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf175-184http://sedici.unlp.edu.ar/handle/10915/145882enginfo:eu-repo/semantics/altIdentifier/issn/1432-1017info:eu-repo/semantics/altIdentifier/issn/0175-7571info:eu-repo/semantics/altIdentifier/doi/10.1007/s00249-008-0365-1info:eu-repo/semantics/altIdentifier/pmid/18769911info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:17Zoai:sedici.unlp.edu.ar:10915/145882Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:18.152SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Analysis of the source of heterogeneity in the osmotic response of plant membrane vesicles |
title |
Analysis of the source of heterogeneity in the osmotic response of plant membrane vesicles |
spellingShingle |
Analysis of the source of heterogeneity in the osmotic response of plant membrane vesicles Alleva, Karina Ciencias Exactas Biología Aquaporins Stopped flow Osmotic permeability Mathematical modeling Simulations Water transport |
title_short |
Analysis of the source of heterogeneity in the osmotic response of plant membrane vesicles |
title_full |
Analysis of the source of heterogeneity in the osmotic response of plant membrane vesicles |
title_fullStr |
Analysis of the source of heterogeneity in the osmotic response of plant membrane vesicles |
title_full_unstemmed |
Analysis of the source of heterogeneity in the osmotic response of plant membrane vesicles |
title_sort |
Analysis of the source of heterogeneity in the osmotic response of plant membrane vesicles |
dc.creator.none.fl_str_mv |
Alleva, Karina Chara, Osvaldo Sutka, Moira Amodeo, Gabriela |
author |
Alleva, Karina |
author_facet |
Alleva, Karina Chara, Osvaldo Sutka, Moira Amodeo, Gabriela |
author_role |
author |
author2 |
Chara, Osvaldo Sutka, Moira Amodeo, Gabriela |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Ciencias Exactas Biología Aquaporins Stopped flow Osmotic permeability Mathematical modeling Simulations Water transport |
topic |
Ciencias Exactas Biología Aquaporins Stopped flow Osmotic permeability Mathematical modeling Simulations Water transport |
dc.description.none.fl_txt_mv |
Plasma membrane vesicles have been widely employed to understand the biophysics of water movements, especially when active aquaporins are present. In general, water permeability coefficients in these preparations outcome from the analysis of the osmotic response of the vesicles by means of light scattering. As from now, this is possible by following a theoretical approach that assumes that scattered light follows a single exponential function and that this behavior is the consequence of vesicle volume changes due to an osmotic challenge. However, some experimental data do not necessarily fit to single exponentials but to double ones. It is argued that the observed double exponential behavior has two possible causes: different vesicle population in terms of permeability or in terms of size distribution. As classical models cannot identify this source of heterogeneity, a mathematical modeling approach was developed based on phenomenological equations of water transport. In the three comparative models presented here, it was assumed that water moves according to an osmotic mechanism across the vesicles, and there is no solute movement across them. Interestingly, when tested in a well described plasma membrane vesicle preparation, the application of these models indicates that the source of heterogeneity in the osmotic response is vesicles having different permeability, clearly discarding the variable size effect. In conclusion, the mathematical approach presented here allows to identify the source of heterogeneity; this information being of particular interest, especially when studying gating mechanisms triggered in water channel activity. Instituto de Física de Líquidos y Sistemas Biológicos |
description |
Plasma membrane vesicles have been widely employed to understand the biophysics of water movements, especially when active aquaporins are present. In general, water permeability coefficients in these preparations outcome from the analysis of the osmotic response of the vesicles by means of light scattering. As from now, this is possible by following a theoretical approach that assumes that scattered light follows a single exponential function and that this behavior is the consequence of vesicle volume changes due to an osmotic challenge. However, some experimental data do not necessarily fit to single exponentials but to double ones. It is argued that the observed double exponential behavior has two possible causes: different vesicle population in terms of permeability or in terms of size distribution. As classical models cannot identify this source of heterogeneity, a mathematical modeling approach was developed based on phenomenological equations of water transport. In the three comparative models presented here, it was assumed that water moves according to an osmotic mechanism across the vesicles, and there is no solute movement across them. Interestingly, when tested in a well described plasma membrane vesicle preparation, the application of these models indicates that the source of heterogeneity in the osmotic response is vesicles having different permeability, clearly discarding the variable size effect. In conclusion, the mathematical approach presented here allows to identify the source of heterogeneity; this information being of particular interest, especially when studying gating mechanisms triggered in water channel activity. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-09-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/145882 |
url |
http://sedici.unlp.edu.ar/handle/10915/145882 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/issn/1432-1017 info:eu-repo/semantics/altIdentifier/issn/0175-7571 info:eu-repo/semantics/altIdentifier/doi/10.1007/s00249-008-0365-1 info:eu-repo/semantics/altIdentifier/pmid/18769911 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
dc.format.none.fl_str_mv |
application/pdf 175-184 |
dc.source.none.fl_str_mv |
reponame:SEDICI (UNLP) instname:Universidad Nacional de La Plata instacron:UNLP |
reponame_str |
SEDICI (UNLP) |
collection |
SEDICI (UNLP) |
instname_str |
Universidad Nacional de La Plata |
instacron_str |
UNLP |
institution |
UNLP |
repository.name.fl_str_mv |
SEDICI (UNLP) - Universidad Nacional de La Plata |
repository.mail.fl_str_mv |
alira@sedici.unlp.edu.ar |
_version_ |
1844616202709630976 |
score |
13.070432 |