DNA content and expression of cell cycle proteins in caterpillar nuclei from fetal human cardiac myocytes

Autores
García Rivello, Hernán Jorge; Cabeza Meckert, Patricia Mildred; Gallo, Amelia; Palacios, Luis; Laguens, Rubén P.
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Caterpillar nuclei (CN) are characterized by their peculiar morphology, with chromatin distributed in clusters and running along the longitudinal axis of the nucleus. They can be observed in normal hearts of fetuses as well as in hearts of children and adults with rheumatic heart disease. This study has demonstrated by means of ploidy studies with digital image analysis that in the fetal heart (20.5±1.8 weeks) the CN (diploid = 5.6±8.4%; tetraploid = 46.2±24.2%; hypertetraploid = 46.9±26.3%) present higher DNA content than non-caterpillar myocyte nuclei (diploid = 89.4±6.2%; tetraploid = 10.0±4.1%; hypertetraploid = 1.5±1.3%) (P=0.000001, 0.00013, and 0.000038, respectively). Expression of proliferation cell nuclear antigen (PCNA; 30.6±11.7% in CN and 13.4±7.3% in non-caterpillar myocyte nuclei; P=0.0115) and cyclin B1 (2.8±3.8% and 12.6±15.6%, respectively; P=n.s.) was also positive in these nuclei. In conclusion, these results suggest that there exists a relationship between CN morphology and myocyte replication phenomena.
Universidad Nacional de La Plata
Materia
Medicina
Caterpillar nuclei
Cyclin B1
Fetal heart
Nuclear ploidy
PCNA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/134685
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/134685
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling DNA content and expression of cell cycle proteins in caterpillar nuclei from fetal human cardiac myocytesGarcía Rivello, Hernán JorgeCabeza Meckert, Patricia MildredGallo, AmeliaPalacios, LuisLaguens, Rubén P.MedicinaCaterpillar nucleiCyclin B1Fetal heartNuclear ploidyPCNACaterpillar nuclei (CN) are characterized by their peculiar morphology, with chromatin distributed in clusters and running along the longitudinal axis of the nucleus. They can be observed in normal hearts of fetuses as well as in hearts of children and adults with rheumatic heart disease. This study has demonstrated by means of ploidy studies with digital image analysis that in the fetal heart (20.5±1.8 weeks) the CN (diploid = 5.6±8.4%; tetraploid = 46.2±24.2%; hypertetraploid = 46.9±26.3%) present higher DNA content than non-caterpillar myocyte nuclei (diploid = 89.4±6.2%; tetraploid = 10.0±4.1%; hypertetraploid = 1.5±1.3%) (P=0.000001, 0.00013, and 0.000038, respectively). Expression of proliferation cell nuclear antigen (PCNA; 30.6±11.7% in CN and 13.4±7.3% in non-caterpillar myocyte nuclei; P=0.0115) and cyclin B1 (2.8±3.8% and 12.6±15.6%, respectively; P=n.s.) was also positive in these nuclei. In conclusion, these results suggest that there exists a relationship between CN morphology and myocyte replication phenomena.Universidad Nacional de La Plata2002info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf45-49http://sedici.unlp.edu.ar/handle/10915/134685enginfo:eu-repo/semantics/altIdentifier/issn/0945-6317info:eu-repo/semantics/altIdentifier/issn/1432-2307info:eu-repo/semantics/altIdentifier/doi/10.1007/s004280100486info:eu-repo/semantics/altIdentifier/pmid/11942576info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-29T15:36:59Zoai:sedici.unlp.edu.ar:10915/134685Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-29 15:36:59.559SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv DNA content and expression of cell cycle proteins in caterpillar nuclei from fetal human cardiac myocytes
title DNA content and expression of cell cycle proteins in caterpillar nuclei from fetal human cardiac myocytes
spellingShingle DNA content and expression of cell cycle proteins in caterpillar nuclei from fetal human cardiac myocytes
García Rivello, Hernán Jorge
Medicina
Caterpillar nuclei
Cyclin B1
Fetal heart
Nuclear ploidy
PCNA
title_short DNA content and expression of cell cycle proteins in caterpillar nuclei from fetal human cardiac myocytes
title_full DNA content and expression of cell cycle proteins in caterpillar nuclei from fetal human cardiac myocytes
title_fullStr DNA content and expression of cell cycle proteins in caterpillar nuclei from fetal human cardiac myocytes
title_full_unstemmed DNA content and expression of cell cycle proteins in caterpillar nuclei from fetal human cardiac myocytes
title_sort DNA content and expression of cell cycle proteins in caterpillar nuclei from fetal human cardiac myocytes
dc.creator.none.fl_str_mv García Rivello, Hernán Jorge
Cabeza Meckert, Patricia Mildred
Gallo, Amelia
Palacios, Luis
Laguens, Rubén P.
author García Rivello, Hernán Jorge
author_facet García Rivello, Hernán Jorge
Cabeza Meckert, Patricia Mildred
Gallo, Amelia
Palacios, Luis
Laguens, Rubén P.
author_role author
author2 Cabeza Meckert, Patricia Mildred
Gallo, Amelia
Palacios, Luis
Laguens, Rubén P.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Medicina
Caterpillar nuclei
Cyclin B1
Fetal heart
Nuclear ploidy
PCNA
topic Medicina
Caterpillar nuclei
Cyclin B1
Fetal heart
Nuclear ploidy
PCNA
dc.description.none.fl_txt_mv Caterpillar nuclei (CN) are characterized by their peculiar morphology, with chromatin distributed in clusters and running along the longitudinal axis of the nucleus. They can be observed in normal hearts of fetuses as well as in hearts of children and adults with rheumatic heart disease. This study has demonstrated by means of ploidy studies with digital image analysis that in the fetal heart (20.5±1.8 weeks) the CN (diploid = 5.6±8.4%; tetraploid = 46.2±24.2%; hypertetraploid = 46.9±26.3%) present higher DNA content than non-caterpillar myocyte nuclei (diploid = 89.4±6.2%; tetraploid = 10.0±4.1%; hypertetraploid = 1.5±1.3%) (P=0.000001, 0.00013, and 0.000038, respectively). Expression of proliferation cell nuclear antigen (PCNA; 30.6±11.7% in CN and 13.4±7.3% in non-caterpillar myocyte nuclei; P=0.0115) and cyclin B1 (2.8±3.8% and 12.6±15.6%, respectively; P=n.s.) was also positive in these nuclei. In conclusion, these results suggest that there exists a relationship between CN morphology and myocyte replication phenomena.
Universidad Nacional de La Plata
description Caterpillar nuclei (CN) are characterized by their peculiar morphology, with chromatin distributed in clusters and running along the longitudinal axis of the nucleus. They can be observed in normal hearts of fetuses as well as in hearts of children and adults with rheumatic heart disease. This study has demonstrated by means of ploidy studies with digital image analysis that in the fetal heart (20.5±1.8 weeks) the CN (diploid = 5.6±8.4%; tetraploid = 46.2±24.2%; hypertetraploid = 46.9±26.3%) present higher DNA content than non-caterpillar myocyte nuclei (diploid = 89.4±6.2%; tetraploid = 10.0±4.1%; hypertetraploid = 1.5±1.3%) (P=0.000001, 0.00013, and 0.000038, respectively). Expression of proliferation cell nuclear antigen (PCNA; 30.6±11.7% in CN and 13.4±7.3% in non-caterpillar myocyte nuclei; P=0.0115) and cyclin B1 (2.8±3.8% and 12.6±15.6%, respectively; P=n.s.) was also positive in these nuclei. In conclusion, these results suggest that there exists a relationship between CN morphology and myocyte replication phenomena.
publishDate 2002
dc.date.none.fl_str_mv 2002
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/134685
url http://sedici.unlp.edu.ar/handle/10915/134685
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0945-6317
info:eu-repo/semantics/altIdentifier/issn/1432-2307
info:eu-repo/semantics/altIdentifier/doi/10.1007/s004280100486
info:eu-repo/semantics/altIdentifier/pmid/11942576
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
45-49
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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