Pseudomonas populations causing pith necrosis of tomato and pepper in Argentina are highly diverse

Autores
Alippi, Adriana Mónica; Dal Bó, Elena; Ronco, Blanca Lía; López, María Virginia; López, Ana Claudia; Aguilar, Orlando Mario
Año de publicación
2003
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pseudomonas species causing pith necrosis symptoms on tomato and pepper collected in different areas of Argentina were identified as Pseudomonas corrugata, P. viridiflava and Pseudomonas spp. Their diversity was analysed and compared with reference strains on the basis of their phenotypic characteristics, copper and antibiotic sensitivity tests, serology, pathogenicity, DNA fingerprinting and restriction fragment length polymorphism (RFLP) analysis of a 16S rRNA gene fragment. All P. corrugata strains tested were copper-resistant while P. viridiflava strains were more variable. Numerical analysis of phenotypic data showed that all P. corrugata strains formed a single phenon that clustered at a level of about 93%, while all the P. viridiflava strains clustered in a separated phenon at a level of 94%. Genomic analysis by repetitive (rep)-PCR and 16S rRNA-RFLP fingerprinting and serological analysis showed that the two species contained considerable genetic diversity. Inoculations of tomato and pepper plants with strains from both hosts caused similar pith necrosis symptoms. Strains of both P. corrugata and P. viridiflava were grouped according to their geographical origin and not according to the original host. This is the first report of Pseudomonas viridiflava causing pith necrosis on pepper.
Facultad de Ciencias Agrarias y Forestales
Instituto de Biotecnologia y Biologia Molecular
Materia
Ciencias Agrarias
Copper resistance
Pathogenicity
Pepper
Pseudomonas corrugata
Pseudomonas viridiflava
Tomato pith necrosis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/83291
Acceder
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network_name_str SEDICI (UNLP)
spelling Pseudomonas populations causing pith necrosis of tomato and pepper in Argentina are highly diverseAlippi, Adriana MónicaDal Bó, ElenaRonco, Blanca LíaLópez, María VirginiaLópez, Ana ClaudiaAguilar, Orlando MarioCiencias AgrariasCopper resistancePathogenicityPepperPseudomonas corrugataPseudomonas viridiflavaTomato pith necrosisPseudomonas species causing pith necrosis symptoms on tomato and pepper collected in different areas of Argentina were identified as Pseudomonas corrugata, P. viridiflava and Pseudomonas spp. Their diversity was analysed and compared with reference strains on the basis of their phenotypic characteristics, copper and antibiotic sensitivity tests, serology, pathogenicity, DNA fingerprinting and restriction fragment length polymorphism (RFLP) analysis of a 16S rRNA gene fragment. All P. corrugata strains tested were copper-resistant while P. viridiflava strains were more variable. Numerical analysis of phenotypic data showed that all P. corrugata strains formed a single phenon that clustered at a level of about 93%, while all the P. viridiflava strains clustered in a separated phenon at a level of 94%. Genomic analysis by repetitive (rep)-PCR and 16S rRNA-RFLP fingerprinting and serological analysis showed that the two species contained considerable genetic diversity. Inoculations of tomato and pepper plants with strains from both hosts caused similar pith necrosis symptoms. Strains of both P. corrugata and P. viridiflava were grouped according to their geographical origin and not according to the original host. This is the first report of Pseudomonas viridiflava causing pith necrosis on pepper.Facultad de Ciencias Agrarias y ForestalesInstituto de Biotecnologia y Biologia Molecular2003info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf287-302http://sedici.unlp.edu.ar/handle/10915/83291enginfo:eu-repo/semantics/altIdentifier/issn/0032-0862info:eu-repo/semantics/altIdentifier/doi/10.1046/j.1365-3059.2003.00850.xinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:15:50Zoai:sedici.unlp.edu.ar:10915/83291Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:15:50.824SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Pseudomonas populations causing pith necrosis of tomato and pepper in Argentina are highly diverse
title Pseudomonas populations causing pith necrosis of tomato and pepper in Argentina are highly diverse
spellingShingle Pseudomonas populations causing pith necrosis of tomato and pepper in Argentina are highly diverse
Alippi, Adriana Mónica
Ciencias Agrarias
Copper resistance
Pathogenicity
Pepper
Pseudomonas corrugata
Pseudomonas viridiflava
Tomato pith necrosis
title_short Pseudomonas populations causing pith necrosis of tomato and pepper in Argentina are highly diverse
title_full Pseudomonas populations causing pith necrosis of tomato and pepper in Argentina are highly diverse
title_fullStr Pseudomonas populations causing pith necrosis of tomato and pepper in Argentina are highly diverse
title_full_unstemmed Pseudomonas populations causing pith necrosis of tomato and pepper in Argentina are highly diverse
title_sort Pseudomonas populations causing pith necrosis of tomato and pepper in Argentina are highly diverse
dc.creator.none.fl_str_mv Alippi, Adriana Mónica
Dal Bó, Elena
Ronco, Blanca Lía
López, María Virginia
López, Ana Claudia
Aguilar, Orlando Mario
author Alippi, Adriana Mónica
author_facet Alippi, Adriana Mónica
Dal Bó, Elena
Ronco, Blanca Lía
López, María Virginia
López, Ana Claudia
Aguilar, Orlando Mario
author_role author
author2 Dal Bó, Elena
Ronco, Blanca Lía
López, María Virginia
López, Ana Claudia
Aguilar, Orlando Mario
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Agrarias
Copper resistance
Pathogenicity
Pepper
Pseudomonas corrugata
Pseudomonas viridiflava
Tomato pith necrosis
topic Ciencias Agrarias
Copper resistance
Pathogenicity
Pepper
Pseudomonas corrugata
Pseudomonas viridiflava
Tomato pith necrosis
dc.description.none.fl_txt_mv Pseudomonas species causing pith necrosis symptoms on tomato and pepper collected in different areas of Argentina were identified as Pseudomonas corrugata, P. viridiflava and Pseudomonas spp. Their diversity was analysed and compared with reference strains on the basis of their phenotypic characteristics, copper and antibiotic sensitivity tests, serology, pathogenicity, DNA fingerprinting and restriction fragment length polymorphism (RFLP) analysis of a 16S rRNA gene fragment. All P. corrugata strains tested were copper-resistant while P. viridiflava strains were more variable. Numerical analysis of phenotypic data showed that all P. corrugata strains formed a single phenon that clustered at a level of about 93%, while all the P. viridiflava strains clustered in a separated phenon at a level of 94%. Genomic analysis by repetitive (rep)-PCR and 16S rRNA-RFLP fingerprinting and serological analysis showed that the two species contained considerable genetic diversity. Inoculations of tomato and pepper plants with strains from both hosts caused similar pith necrosis symptoms. Strains of both P. corrugata and P. viridiflava were grouped according to their geographical origin and not according to the original host. This is the first report of Pseudomonas viridiflava causing pith necrosis on pepper.
Facultad de Ciencias Agrarias y Forestales
Instituto de Biotecnologia y Biologia Molecular
description Pseudomonas species causing pith necrosis symptoms on tomato and pepper collected in different areas of Argentina were identified as Pseudomonas corrugata, P. viridiflava and Pseudomonas spp. Their diversity was analysed and compared with reference strains on the basis of their phenotypic characteristics, copper and antibiotic sensitivity tests, serology, pathogenicity, DNA fingerprinting and restriction fragment length polymorphism (RFLP) analysis of a 16S rRNA gene fragment. All P. corrugata strains tested were copper-resistant while P. viridiflava strains were more variable. Numerical analysis of phenotypic data showed that all P. corrugata strains formed a single phenon that clustered at a level of about 93%, while all the P. viridiflava strains clustered in a separated phenon at a level of 94%. Genomic analysis by repetitive (rep)-PCR and 16S rRNA-RFLP fingerprinting and serological analysis showed that the two species contained considerable genetic diversity. Inoculations of tomato and pepper plants with strains from both hosts caused similar pith necrosis symptoms. Strains of both P. corrugata and P. viridiflava were grouped according to their geographical origin and not according to the original host. This is the first report of Pseudomonas viridiflava causing pith necrosis on pepper.
publishDate 2003
dc.date.none.fl_str_mv 2003
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/83291
url http://sedici.unlp.edu.ar/handle/10915/83291
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0032-0862
info:eu-repo/semantics/altIdentifier/doi/10.1046/j.1365-3059.2003.00850.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
287-302
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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