Few amino acid mutations in H6 Influenza A virus from South American Lineage increase viral replication efficiency in poultry

Autores
Rimondi, Agustina; Olivera, Valeria S.; Soria, Ivana; Parisi, Gustavo Daniel; Rumbo, Martín; Pérez, Daniel R.
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In chickens, infections due to influenza A virus (IAV) can be mild to severe and lethal. The study of IAV infections in poultry has been mostly limited to strains from the North American and Eurasian lineages, whereas limited information exists on similar studies with strains from the South American lineage (SAm). To better evaluate the risk of introduction of a prototypical SAm IAV strain into poultry, chickens were infected with a wild-type SAm origin strain (WT557/H6N2). The resulting virus progeny was serially passaged in chickens 20 times, and the immunopathological effects of the last passage virus, 20Ch557/H6N2, in chickens were compared to those of the parental strain. A comparison of complete viral genome sequences indicated that the 20Ch557/H6N2 strain contained 13 amino acid differences compared to the wild-type strain. Five of these mutations are in functionally relevant regions of the viral surface glycoproteins hemagglutinin (HA) and neuraminidase (NA). However, despite higher and more prolonged virus shedding in chickens inoculated with the 20Ch557/H6N2 strain compared to those that received the WT557/H6N2 strain, transmission to naïve chickens was not observed for either group. Analyses by flow cytometry of mononuclear cells and lymphocyte subpopulations from the lamina propria and intraepithelial lymphocytic cells (IELs) from the ileum revealed a significant increase in the percentages of CD3CTCRgdC IELs in chickens inoculated with the 20Ch557/H6N2 strain compared to those inoculated with the WT557/H6N2 strain.
Instituto de Estudios Inmunológicos y Fisiopatológicos
Materia
Biología
Ciencias Veterinarias
influenza A virus
South American IAV
viral adaptation
viral fitness
viral transmission
chicken studies
risk to poultry
immunopathogenesis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/155926
Acceder
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spelling Few amino acid mutations in H6 Influenza A virus from South American Lineage increase viral replication efficiency in poultryRimondi, AgustinaOlivera, Valeria S.Soria, IvanaParisi, Gustavo DanielRumbo, MartínPérez, Daniel R.BiologíaCiencias Veterinariasinfluenza A virusSouth American IAVviral adaptationviral fitnessviral transmissionchicken studiesrisk to poultryimmunopathogenesisIn chickens, infections due to influenza A virus (IAV) can be mild to severe and lethal. The study of IAV infections in poultry has been mostly limited to strains from the North American and Eurasian lineages, whereas limited information exists on similar studies with strains from the South American lineage (SAm). To better evaluate the risk of introduction of a prototypical SAm IAV strain into poultry, chickens were infected with a wild-type SAm origin strain (WT557/H6N2). The resulting virus progeny was serially passaged in chickens 20 times, and the immunopathological effects of the last passage virus, 20Ch557/H6N2, in chickens were compared to those of the parental strain. A comparison of complete viral genome sequences indicated that the 20Ch557/H6N2 strain contained 13 amino acid differences compared to the wild-type strain. Five of these mutations are in functionally relevant regions of the viral surface glycoproteins hemagglutinin (HA) and neuraminidase (NA). However, despite higher and more prolonged virus shedding in chickens inoculated with the 20Ch557/H6N2 strain compared to those that received the WT557/H6N2 strain, transmission to naïve chickens was not observed for either group. Analyses by flow cytometry of mononuclear cells and lymphocyte subpopulations from the lamina propria and intraepithelial lymphocytic cells (IELs) from the ileum revealed a significant increase in the percentages of CD3CTCRgdC IELs in chickens inoculated with the 20Ch557/H6N2 strain compared to those inoculated with the WT557/H6N2 strain.Instituto de Estudios Inmunológicos y Fisiopatológicos2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/155926enginfo:eu-repo/semantics/altIdentifier/issn/1664-302Xinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2022.953738info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:40:27Zoai:sedici.unlp.edu.ar:10915/155926Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:40:28.214SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Few amino acid mutations in H6 Influenza A virus from South American Lineage increase viral replication efficiency in poultry
title Few amino acid mutations in H6 Influenza A virus from South American Lineage increase viral replication efficiency in poultry
spellingShingle Few amino acid mutations in H6 Influenza A virus from South American Lineage increase viral replication efficiency in poultry
Rimondi, Agustina
Biología
Ciencias Veterinarias
influenza A virus
South American IAV
viral adaptation
viral fitness
viral transmission
chicken studies
risk to poultry
immunopathogenesis
title_short Few amino acid mutations in H6 Influenza A virus from South American Lineage increase viral replication efficiency in poultry
title_full Few amino acid mutations in H6 Influenza A virus from South American Lineage increase viral replication efficiency in poultry
title_fullStr Few amino acid mutations in H6 Influenza A virus from South American Lineage increase viral replication efficiency in poultry
title_full_unstemmed Few amino acid mutations in H6 Influenza A virus from South American Lineage increase viral replication efficiency in poultry
title_sort Few amino acid mutations in H6 Influenza A virus from South American Lineage increase viral replication efficiency in poultry
dc.creator.none.fl_str_mv Rimondi, Agustina
Olivera, Valeria S.
Soria, Ivana
Parisi, Gustavo Daniel
Rumbo, Martín
Pérez, Daniel R.
author Rimondi, Agustina
author_facet Rimondi, Agustina
Olivera, Valeria S.
Soria, Ivana
Parisi, Gustavo Daniel
Rumbo, Martín
Pérez, Daniel R.
author_role author
author2 Olivera, Valeria S.
Soria, Ivana
Parisi, Gustavo Daniel
Rumbo, Martín
Pérez, Daniel R.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Ciencias Veterinarias
influenza A virus
South American IAV
viral adaptation
viral fitness
viral transmission
chicken studies
risk to poultry
immunopathogenesis
topic Biología
Ciencias Veterinarias
influenza A virus
South American IAV
viral adaptation
viral fitness
viral transmission
chicken studies
risk to poultry
immunopathogenesis
dc.description.none.fl_txt_mv In chickens, infections due to influenza A virus (IAV) can be mild to severe and lethal. The study of IAV infections in poultry has been mostly limited to strains from the North American and Eurasian lineages, whereas limited information exists on similar studies with strains from the South American lineage (SAm). To better evaluate the risk of introduction of a prototypical SAm IAV strain into poultry, chickens were infected with a wild-type SAm origin strain (WT557/H6N2). The resulting virus progeny was serially passaged in chickens 20 times, and the immunopathological effects of the last passage virus, 20Ch557/H6N2, in chickens were compared to those of the parental strain. A comparison of complete viral genome sequences indicated that the 20Ch557/H6N2 strain contained 13 amino acid differences compared to the wild-type strain. Five of these mutations are in functionally relevant regions of the viral surface glycoproteins hemagglutinin (HA) and neuraminidase (NA). However, despite higher and more prolonged virus shedding in chickens inoculated with the 20Ch557/H6N2 strain compared to those that received the WT557/H6N2 strain, transmission to naïve chickens was not observed for either group. Analyses by flow cytometry of mononuclear cells and lymphocyte subpopulations from the lamina propria and intraepithelial lymphocytic cells (IELs) from the ileum revealed a significant increase in the percentages of CD3CTCRgdC IELs in chickens inoculated with the 20Ch557/H6N2 strain compared to those inoculated with the WT557/H6N2 strain.
Instituto de Estudios Inmunológicos y Fisiopatológicos
description In chickens, infections due to influenza A virus (IAV) can be mild to severe and lethal. The study of IAV infections in poultry has been mostly limited to strains from the North American and Eurasian lineages, whereas limited information exists on similar studies with strains from the South American lineage (SAm). To better evaluate the risk of introduction of a prototypical SAm IAV strain into poultry, chickens were infected with a wild-type SAm origin strain (WT557/H6N2). The resulting virus progeny was serially passaged in chickens 20 times, and the immunopathological effects of the last passage virus, 20Ch557/H6N2, in chickens were compared to those of the parental strain. A comparison of complete viral genome sequences indicated that the 20Ch557/H6N2 strain contained 13 amino acid differences compared to the wild-type strain. Five of these mutations are in functionally relevant regions of the viral surface glycoproteins hemagglutinin (HA) and neuraminidase (NA). However, despite higher and more prolonged virus shedding in chickens inoculated with the 20Ch557/H6N2 strain compared to those that received the WT557/H6N2 strain, transmission to naïve chickens was not observed for either group. Analyses by flow cytometry of mononuclear cells and lymphocyte subpopulations from the lamina propria and intraepithelial lymphocytic cells (IELs) from the ileum revealed a significant increase in the percentages of CD3CTCRgdC IELs in chickens inoculated with the 20Ch557/H6N2 strain compared to those inoculated with the WT557/H6N2 strain.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/155926
url http://sedici.unlp.edu.ar/handle/10915/155926
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1664-302X
info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2022.953738
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
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instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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