Small non-coding RNA landscape is modified by GPAT2 silencing in MDA-MB-231 cells

Autores
Lacunza, Ezequiel; Montanaro, Mauro Aldo; Salvati, Annamaria; Memoli, Domenico; Rizzo, Francesca; Henning, María Florencia; Quiroga, Ivana Yoseli; Guillou, Hervé; Abba, Martín Carlos; González Baró, María del Rosario; Weisz, Alessandro; Pellón Maisón, Magalí
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Glycerol-3-phosphate acyltransferase-2 is a member of “cancer-testis gene” family. Initially linked to lipid metabolism, this gene has been recently found involved also in PIWI-interacting RNAs biogenesis in germline stem cells. To investigate its role in piRNA metabolism in cancer, the gene was silenced in MDA-MB-231 breast cancer cells and small RNA sequencing was applied. PIWI-interacting RNAs and tRNA-derived fragments expression profiles showed changes following GPAT2 silencing. Interestingly, a marked shift in length distribution for both small RNAs was detected in GPAT2-silenced cells. Most downregulated PIWI-interacting RNAs are single copy in the genome, intragenic, hosted in snoRNAs and previously found to be upregulated in cancer cells. Putative targets of these PIWI-interacting RNAs are linked to lipid metabolism. Downregulated tRNA derived fragments derived from, socalled ‘differentiation tRNAs’, whereas upregulated ones derived from proliferationlinked tRNAs. miRNA amounts decrease after Glycerol-3-phosphate acyltransferase-2 silencing and functional enrichment analysis of deregulated miRNA putative targets point to mitochondrial biogenesis, IGF1R signaling and oxidative metabolism of lipids and lipoproteins. In addition, miRNAs known to be overexpressed in breast cancer tumors with poor prognosis where found downregulated in GPAT2-silenced cells. In conclusion, GPAT2 silencing quantitatively and qualitatively affects the population of PIWI-interacting RNAs, tRNA derived fragments and miRNAs which, in combination, result in a more differentiated cancer cell phenotype.
Centro de Investigaciones Inmunológicas Básicas y Aplicadas
Instituto de Investigaciones Bioquímicas de La Plata
Materia
Ciencias Médicas
GPAT2
breast cancer
piRNAs
tRNA derived fragments
small non-coding RNAs
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/3.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/107772
Acceder
id SEDICI_d84666a40b50aa3bddca7621f5fae18d
oai_identifier_str oai:sedici.unlp.edu.ar:10915/107772
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Small non-coding RNA landscape is modified by GPAT2 silencing in MDA-MB-231 cellsLacunza, EzequielMontanaro, Mauro AldoSalvati, AnnamariaMemoli, DomenicoRizzo, FrancescaHenning, María FlorenciaQuiroga, Ivana YoseliGuillou, HervéAbba, Martín CarlosGonzález Baró, María del RosarioWeisz, AlessandroPellón Maisón, MagalíCiencias MédicasGPAT2breast cancerpiRNAstRNA derived fragmentssmall non-coding RNAsGlycerol-3-phosphate acyltransferase-2 is a member of “cancer-testis gene” family. Initially linked to lipid metabolism, this gene has been recently found involved also in PIWI-interacting RNAs biogenesis in germline stem cells. To investigate its role in piRNA metabolism in cancer, the gene was silenced in MDA-MB-231 breast cancer cells and small RNA sequencing was applied. PIWI-interacting RNAs and tRNA-derived fragments expression profiles showed changes following GPAT2 silencing. Interestingly, a marked shift in length distribution for both small RNAs was detected in GPAT2-silenced cells. Most downregulated PIWI-interacting RNAs are single copy in the genome, intragenic, hosted in snoRNAs and previously found to be upregulated in cancer cells. Putative targets of these PIWI-interacting RNAs are linked to lipid metabolism. Downregulated tRNA derived fragments derived from, socalled ‘differentiation tRNAs’, whereas upregulated ones derived from proliferationlinked tRNAs. miRNA amounts decrease after Glycerol-3-phosphate acyltransferase-2 silencing and functional enrichment analysis of deregulated miRNA putative targets point to mitochondrial biogenesis, IGF1R signaling and oxidative metabolism of lipids and lipoproteins. In addition, miRNAs known to be overexpressed in breast cancer tumors with poor prognosis where found downregulated in GPAT2-silenced cells. In conclusion, GPAT2 silencing quantitatively and qualitatively affects the population of PIWI-interacting RNAs, tRNA derived fragments and miRNAs which, in combination, result in a more differentiated cancer cell phenotype.Centro de Investigaciones Inmunológicas Básicas y AplicadasInstituto de Investigaciones Bioquímicas de La Plata2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf28141-28154http://sedici.unlp.edu.ar/handle/10915/107772enginfo:eu-repo/semantics/altIdentifier/url/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6021339&blobtype=pdfinfo:eu-repo/semantics/altIdentifier/issn/1949-2553info:eu-repo/semantics/altIdentifier/pmid/29963267info:eu-repo/semantics/altIdentifier/doi/10.18632/oncotarget.25582info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/Creative Commons Attribution 3.0 Unported (CC BY 3.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:56:07Zoai:sedici.unlp.edu.ar:10915/107772Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:56:07.688SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Small non-coding RNA landscape is modified by GPAT2 silencing in MDA-MB-231 cells
title Small non-coding RNA landscape is modified by GPAT2 silencing in MDA-MB-231 cells
spellingShingle Small non-coding RNA landscape is modified by GPAT2 silencing in MDA-MB-231 cells
Lacunza, Ezequiel
Ciencias Médicas
GPAT2
breast cancer
piRNAs
tRNA derived fragments
small non-coding RNAs
title_short Small non-coding RNA landscape is modified by GPAT2 silencing in MDA-MB-231 cells
title_full Small non-coding RNA landscape is modified by GPAT2 silencing in MDA-MB-231 cells
title_fullStr Small non-coding RNA landscape is modified by GPAT2 silencing in MDA-MB-231 cells
title_full_unstemmed Small non-coding RNA landscape is modified by GPAT2 silencing in MDA-MB-231 cells
title_sort Small non-coding RNA landscape is modified by GPAT2 silencing in MDA-MB-231 cells
dc.creator.none.fl_str_mv Lacunza, Ezequiel
Montanaro, Mauro Aldo
Salvati, Annamaria
Memoli, Domenico
Rizzo, Francesca
Henning, María Florencia
Quiroga, Ivana Yoseli
Guillou, Hervé
Abba, Martín Carlos
González Baró, María del Rosario
Weisz, Alessandro
Pellón Maisón, Magalí
author Lacunza, Ezequiel
author_facet Lacunza, Ezequiel
Montanaro, Mauro Aldo
Salvati, Annamaria
Memoli, Domenico
Rizzo, Francesca
Henning, María Florencia
Quiroga, Ivana Yoseli
Guillou, Hervé
Abba, Martín Carlos
González Baró, María del Rosario
Weisz, Alessandro
Pellón Maisón, Magalí
author_role author
author2 Montanaro, Mauro Aldo
Salvati, Annamaria
Memoli, Domenico
Rizzo, Francesca
Henning, María Florencia
Quiroga, Ivana Yoseli
Guillou, Hervé
Abba, Martín Carlos
González Baró, María del Rosario
Weisz, Alessandro
Pellón Maisón, Magalí
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Médicas
GPAT2
breast cancer
piRNAs
tRNA derived fragments
small non-coding RNAs
topic Ciencias Médicas
GPAT2
breast cancer
piRNAs
tRNA derived fragments
small non-coding RNAs
dc.description.none.fl_txt_mv Glycerol-3-phosphate acyltransferase-2 is a member of “cancer-testis gene” family. Initially linked to lipid metabolism, this gene has been recently found involved also in PIWI-interacting RNAs biogenesis in germline stem cells. To investigate its role in piRNA metabolism in cancer, the gene was silenced in MDA-MB-231 breast cancer cells and small RNA sequencing was applied. PIWI-interacting RNAs and tRNA-derived fragments expression profiles showed changes following GPAT2 silencing. Interestingly, a marked shift in length distribution for both small RNAs was detected in GPAT2-silenced cells. Most downregulated PIWI-interacting RNAs are single copy in the genome, intragenic, hosted in snoRNAs and previously found to be upregulated in cancer cells. Putative targets of these PIWI-interacting RNAs are linked to lipid metabolism. Downregulated tRNA derived fragments derived from, socalled ‘differentiation tRNAs’, whereas upregulated ones derived from proliferationlinked tRNAs. miRNA amounts decrease after Glycerol-3-phosphate acyltransferase-2 silencing and functional enrichment analysis of deregulated miRNA putative targets point to mitochondrial biogenesis, IGF1R signaling and oxidative metabolism of lipids and lipoproteins. In addition, miRNAs known to be overexpressed in breast cancer tumors with poor prognosis where found downregulated in GPAT2-silenced cells. In conclusion, GPAT2 silencing quantitatively and qualitatively affects the population of PIWI-interacting RNAs, tRNA derived fragments and miRNAs which, in combination, result in a more differentiated cancer cell phenotype.
Centro de Investigaciones Inmunológicas Básicas y Aplicadas
Instituto de Investigaciones Bioquímicas de La Plata
description Glycerol-3-phosphate acyltransferase-2 is a member of “cancer-testis gene” family. Initially linked to lipid metabolism, this gene has been recently found involved also in PIWI-interacting RNAs biogenesis in germline stem cells. To investigate its role in piRNA metabolism in cancer, the gene was silenced in MDA-MB-231 breast cancer cells and small RNA sequencing was applied. PIWI-interacting RNAs and tRNA-derived fragments expression profiles showed changes following GPAT2 silencing. Interestingly, a marked shift in length distribution for both small RNAs was detected in GPAT2-silenced cells. Most downregulated PIWI-interacting RNAs are single copy in the genome, intragenic, hosted in snoRNAs and previously found to be upregulated in cancer cells. Putative targets of these PIWI-interacting RNAs are linked to lipid metabolism. Downregulated tRNA derived fragments derived from, socalled ‘differentiation tRNAs’, whereas upregulated ones derived from proliferationlinked tRNAs. miRNA amounts decrease after Glycerol-3-phosphate acyltransferase-2 silencing and functional enrichment analysis of deregulated miRNA putative targets point to mitochondrial biogenesis, IGF1R signaling and oxidative metabolism of lipids and lipoproteins. In addition, miRNAs known to be overexpressed in breast cancer tumors with poor prognosis where found downregulated in GPAT2-silenced cells. In conclusion, GPAT2 silencing quantitatively and qualitatively affects the population of PIWI-interacting RNAs, tRNA derived fragments and miRNAs which, in combination, result in a more differentiated cancer cell phenotype.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/107772
url http://sedici.unlp.edu.ar/handle/10915/107772
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6021339&blobtype=pdf
info:eu-repo/semantics/altIdentifier/issn/1949-2553
info:eu-repo/semantics/altIdentifier/pmid/29963267
info:eu-repo/semantics/altIdentifier/doi/10.18632/oncotarget.25582
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/
Creative Commons Attribution 3.0 Unported (CC BY 3.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/
Creative Commons Attribution 3.0 Unported (CC BY 3.0)
dc.format.none.fl_str_mv application/pdf
28141-28154
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
_version_ 1842260448333791232
score 13.13397

Publicaciones similares