Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus Oocytes
- Autores
- Lizarraga, Raúl Martín; Anchordoquy, Juan Mateo; Galarza, Esteban Martín; Farnetano, Nicolás Agustín; Carranza Martín, Ana Cristina; Furnus, Cecilia Cristina; Mattioli, Guillermo Alberto; Anchordoquy, Juan Patricio
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Selenium (Se) is an essential trace element with important functions in animals and whose deficiency is associated with reproductive failures. The aim of this study was to investigate the effect of Se concentrations during in vitro maturation (IVM) of Bos taurus oocyte within the reference ranges for Se status in cattle. For this purpose, Aberdeen Angus cumulus–oocyte complexes (COCs) were matured in IVM medium supplemented with 0, 10, 50, and 100 ng/mL Se (control, deficient, marginal, and adequate, respectively). The results demonstrated that marginal and adequate Se concentrations added during IVM increased viability and non-apoptotic cumulus cells (CC). Moreover, the addition of Se to culture media decreased malondialdehyde level in COC with all studied concentrations and increased total glutathione content in CC and oocytes with 10 ng/mL Se. On the other hand, total antioxidant capacity of COC, nuclear maturation, and the developmental capacity of oocytes were not modified by Se supplementation. However, 10 ng/mL Se increased hatching rate. In conclusion, supplementation with 10 ng/mL Se during in vitro maturation of Bos primigenius taurus oocytes should be considered to improve embryo quality.
Instituto de Genética Veterinaria - Materia
-
Veterinaria
Selenium
MDA
TAC
Apoptosis
GSH
Embryo development - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/141479
Ver los metadatos del registro completo
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Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus OocytesLizarraga, Raúl MartínAnchordoquy, Juan MateoGalarza, Esteban MartínFarnetano, Nicolás AgustínCarranza Martín, Ana CristinaFurnus, Cecilia CristinaMattioli, Guillermo AlbertoAnchordoquy, Juan PatricioVeterinariaSeleniumMDATACApoptosisGSHEmbryo developmentSelenium (Se) is an essential trace element with important functions in animals and whose deficiency is associated with reproductive failures. The aim of this study was to investigate the effect of Se concentrations during in vitro maturation (IVM) of Bos taurus oocyte within the reference ranges for Se status in cattle. For this purpose, Aberdeen Angus cumulus–oocyte complexes (COCs) were matured in IVM medium supplemented with 0, 10, 50, and 100 ng/mL Se (control, deficient, marginal, and adequate, respectively). The results demonstrated that marginal and adequate Se concentrations added during IVM increased viability and non-apoptotic cumulus cells (CC). Moreover, the addition of Se to culture media decreased malondialdehyde level in COC with all studied concentrations and increased total glutathione content in CC and oocytes with 10 ng/mL Se. On the other hand, total antioxidant capacity of COC, nuclear maturation, and the developmental capacity of oocytes were not modified by Se supplementation. However, 10 ng/mL Se increased hatching rate. In conclusion, supplementation with 10 ng/mL Se during in vitro maturation of Bos primigenius taurus oocytes should be considered to improve embryo quality.Instituto de Genética Veterinaria2019-11-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf149-158http://sedici.unlp.edu.ar/handle/10915/141479enginfo:eu-repo/semantics/altIdentifier/issn/1559-0720info:eu-repo/semantics/altIdentifier/issn/0163-4984info:eu-repo/semantics/altIdentifier/doi/10.1007/s12011-019-01966-2info:eu-repo/semantics/altIdentifier/pmid/31705431info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:07Zoai:sedici.unlp.edu.ar:10915/141479Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:08.22SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus Oocytes |
title |
Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus Oocytes |
spellingShingle |
Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus Oocytes Lizarraga, Raúl Martín Veterinaria Selenium MDA TAC Apoptosis GSH Embryo development |
title_short |
Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus Oocytes |
title_full |
Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus Oocytes |
title_fullStr |
Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus Oocytes |
title_full_unstemmed |
Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus Oocytes |
title_sort |
Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus Oocytes |
dc.creator.none.fl_str_mv |
Lizarraga, Raúl Martín Anchordoquy, Juan Mateo Galarza, Esteban Martín Farnetano, Nicolás Agustín Carranza Martín, Ana Cristina Furnus, Cecilia Cristina Mattioli, Guillermo Alberto Anchordoquy, Juan Patricio |
author |
Lizarraga, Raúl Martín |
author_facet |
Lizarraga, Raúl Martín Anchordoquy, Juan Mateo Galarza, Esteban Martín Farnetano, Nicolás Agustín Carranza Martín, Ana Cristina Furnus, Cecilia Cristina Mattioli, Guillermo Alberto Anchordoquy, Juan Patricio |
author_role |
author |
author2 |
Anchordoquy, Juan Mateo Galarza, Esteban Martín Farnetano, Nicolás Agustín Carranza Martín, Ana Cristina Furnus, Cecilia Cristina Mattioli, Guillermo Alberto Anchordoquy, Juan Patricio |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
Veterinaria Selenium MDA TAC Apoptosis GSH Embryo development |
topic |
Veterinaria Selenium MDA TAC Apoptosis GSH Embryo development |
dc.description.none.fl_txt_mv |
Selenium (Se) is an essential trace element with important functions in animals and whose deficiency is associated with reproductive failures. The aim of this study was to investigate the effect of Se concentrations during in vitro maturation (IVM) of Bos taurus oocyte within the reference ranges for Se status in cattle. For this purpose, Aberdeen Angus cumulus–oocyte complexes (COCs) were matured in IVM medium supplemented with 0, 10, 50, and 100 ng/mL Se (control, deficient, marginal, and adequate, respectively). The results demonstrated that marginal and adequate Se concentrations added during IVM increased viability and non-apoptotic cumulus cells (CC). Moreover, the addition of Se to culture media decreased malondialdehyde level in COC with all studied concentrations and increased total glutathione content in CC and oocytes with 10 ng/mL Se. On the other hand, total antioxidant capacity of COC, nuclear maturation, and the developmental capacity of oocytes were not modified by Se supplementation. However, 10 ng/mL Se increased hatching rate. In conclusion, supplementation with 10 ng/mL Se during in vitro maturation of Bos primigenius taurus oocytes should be considered to improve embryo quality. Instituto de Genética Veterinaria |
description |
Selenium (Se) is an essential trace element with important functions in animals and whose deficiency is associated with reproductive failures. The aim of this study was to investigate the effect of Se concentrations during in vitro maturation (IVM) of Bos taurus oocyte within the reference ranges for Se status in cattle. For this purpose, Aberdeen Angus cumulus–oocyte complexes (COCs) were matured in IVM medium supplemented with 0, 10, 50, and 100 ng/mL Se (control, deficient, marginal, and adequate, respectively). The results demonstrated that marginal and adequate Se concentrations added during IVM increased viability and non-apoptotic cumulus cells (CC). Moreover, the addition of Se to culture media decreased malondialdehyde level in COC with all studied concentrations and increased total glutathione content in CC and oocytes with 10 ng/mL Se. On the other hand, total antioxidant capacity of COC, nuclear maturation, and the developmental capacity of oocytes were not modified by Se supplementation. However, 10 ng/mL Se increased hatching rate. In conclusion, supplementation with 10 ng/mL Se during in vitro maturation of Bos primigenius taurus oocytes should be considered to improve embryo quality. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-11-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/141479 |
url |
http://sedici.unlp.edu.ar/handle/10915/141479 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/issn/1559-0720 info:eu-repo/semantics/altIdentifier/issn/0163-4984 info:eu-repo/semantics/altIdentifier/doi/10.1007/s12011-019-01966-2 info:eu-repo/semantics/altIdentifier/pmid/31705431 |
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info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
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application/pdf 149-158 |
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