Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus Oocytes

Autores
Lizarraga, Raúl Martín; Anchordoquy, Juan Mateo; Galarza, Esteban Martín; Farnetano, Nicolás Agustín; Carranza Martín, Ana Cristina; Furnus, Cecilia Cristina; Mattioli, Guillermo Alberto; Anchordoquy, Juan Patricio
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Selenium (Se) is an essential trace element with important functions in animals and whose deficiency is associated with reproductive failures. The aim of this study was to investigate the effect of Se concentrations during in vitro maturation (IVM) of Bos taurus oocyte within the reference ranges for Se status in cattle. For this purpose, Aberdeen Angus cumulus–oocyte complexes (COCs) were matured in IVM medium supplemented with 0, 10, 50, and 100 ng/mL Se (control, deficient, marginal, and adequate, respectively). The results demonstrated that marginal and adequate Se concentrations added during IVM increased viability and non-apoptotic cumulus cells (CC). Moreover, the addition of Se to culture media decreased malondialdehyde level in COC with all studied concentrations and increased total glutathione content in CC and oocytes with 10 ng/mL Se. On the other hand, total antioxidant capacity of COC, nuclear maturation, and the developmental capacity of oocytes were not modified by Se supplementation. However, 10 ng/mL Se increased hatching rate. In conclusion, supplementation with 10 ng/mL Se during in vitro maturation of Bos primigenius taurus oocytes should be considered to improve embryo quality.
Instituto de Genética Veterinaria
Materia
Veterinaria
Selenium
MDA
TAC
Apoptosis
GSH
Embryo development
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/141479
Acceder
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repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus OocytesLizarraga, Raúl MartínAnchordoquy, Juan MateoGalarza, Esteban MartínFarnetano, Nicolás AgustínCarranza Martín, Ana CristinaFurnus, Cecilia CristinaMattioli, Guillermo AlbertoAnchordoquy, Juan PatricioVeterinariaSeleniumMDATACApoptosisGSHEmbryo developmentSelenium (Se) is an essential trace element with important functions in animals and whose deficiency is associated with reproductive failures. The aim of this study was to investigate the effect of Se concentrations during in vitro maturation (IVM) of Bos taurus oocyte within the reference ranges for Se status in cattle. For this purpose, Aberdeen Angus cumulus–oocyte complexes (COCs) were matured in IVM medium supplemented with 0, 10, 50, and 100 ng/mL Se (control, deficient, marginal, and adequate, respectively). The results demonstrated that marginal and adequate Se concentrations added during IVM increased viability and non-apoptotic cumulus cells (CC). Moreover, the addition of Se to culture media decreased malondialdehyde level in COC with all studied concentrations and increased total glutathione content in CC and oocytes with 10 ng/mL Se. On the other hand, total antioxidant capacity of COC, nuclear maturation, and the developmental capacity of oocytes were not modified by Se supplementation. However, 10 ng/mL Se increased hatching rate. In conclusion, supplementation with 10 ng/mL Se during in vitro maturation of Bos primigenius taurus oocytes should be considered to improve embryo quality.Instituto de Genética Veterinaria2019-11-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf149-158http://sedici.unlp.edu.ar/handle/10915/141479enginfo:eu-repo/semantics/altIdentifier/issn/1559-0720info:eu-repo/semantics/altIdentifier/issn/0163-4984info:eu-repo/semantics/altIdentifier/doi/10.1007/s12011-019-01966-2info:eu-repo/semantics/altIdentifier/pmid/31705431info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-22T17:13:02Zoai:sedici.unlp.edu.ar:10915/141479Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-22 17:13:02.55SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus Oocytes
title Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus Oocytes
spellingShingle Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus Oocytes
Lizarraga, Raúl Martín
Veterinaria
Selenium
MDA
TAC
Apoptosis
GSH
Embryo development
title_short Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus Oocytes
title_full Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus Oocytes
title_fullStr Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus Oocytes
title_full_unstemmed Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus Oocytes
title_sort Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus Oocytes
dc.creator.none.fl_str_mv Lizarraga, Raúl Martín
Anchordoquy, Juan Mateo
Galarza, Esteban Martín
Farnetano, Nicolás Agustín
Carranza Martín, Ana Cristina
Furnus, Cecilia Cristina
Mattioli, Guillermo Alberto
Anchordoquy, Juan Patricio
author Lizarraga, Raúl Martín
author_facet Lizarraga, Raúl Martín
Anchordoquy, Juan Mateo
Galarza, Esteban Martín
Farnetano, Nicolás Agustín
Carranza Martín, Ana Cristina
Furnus, Cecilia Cristina
Mattioli, Guillermo Alberto
Anchordoquy, Juan Patricio
author_role author
author2 Anchordoquy, Juan Mateo
Galarza, Esteban Martín
Farnetano, Nicolás Agustín
Carranza Martín, Ana Cristina
Furnus, Cecilia Cristina
Mattioli, Guillermo Alberto
Anchordoquy, Juan Patricio
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Veterinaria
Selenium
MDA
TAC
Apoptosis
GSH
Embryo development
topic Veterinaria
Selenium
MDA
TAC
Apoptosis
GSH
Embryo development
dc.description.none.fl_txt_mv Selenium (Se) is an essential trace element with important functions in animals and whose deficiency is associated with reproductive failures. The aim of this study was to investigate the effect of Se concentrations during in vitro maturation (IVM) of Bos taurus oocyte within the reference ranges for Se status in cattle. For this purpose, Aberdeen Angus cumulus–oocyte complexes (COCs) were matured in IVM medium supplemented with 0, 10, 50, and 100 ng/mL Se (control, deficient, marginal, and adequate, respectively). The results demonstrated that marginal and adequate Se concentrations added during IVM increased viability and non-apoptotic cumulus cells (CC). Moreover, the addition of Se to culture media decreased malondialdehyde level in COC with all studied concentrations and increased total glutathione content in CC and oocytes with 10 ng/mL Se. On the other hand, total antioxidant capacity of COC, nuclear maturation, and the developmental capacity of oocytes were not modified by Se supplementation. However, 10 ng/mL Se increased hatching rate. In conclusion, supplementation with 10 ng/mL Se during in vitro maturation of Bos primigenius taurus oocytes should be considered to improve embryo quality.
Instituto de Genética Veterinaria
description Selenium (Se) is an essential trace element with important functions in animals and whose deficiency is associated with reproductive failures. The aim of this study was to investigate the effect of Se concentrations during in vitro maturation (IVM) of Bos taurus oocyte within the reference ranges for Se status in cattle. For this purpose, Aberdeen Angus cumulus–oocyte complexes (COCs) were matured in IVM medium supplemented with 0, 10, 50, and 100 ng/mL Se (control, deficient, marginal, and adequate, respectively). The results demonstrated that marginal and adequate Se concentrations added during IVM increased viability and non-apoptotic cumulus cells (CC). Moreover, the addition of Se to culture media decreased malondialdehyde level in COC with all studied concentrations and increased total glutathione content in CC and oocytes with 10 ng/mL Se. On the other hand, total antioxidant capacity of COC, nuclear maturation, and the developmental capacity of oocytes were not modified by Se supplementation. However, 10 ng/mL Se increased hatching rate. In conclusion, supplementation with 10 ng/mL Se during in vitro maturation of Bos primigenius taurus oocytes should be considered to improve embryo quality.
publishDate 2019
dc.date.none.fl_str_mv 2019-11-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/141479
url http://sedici.unlp.edu.ar/handle/10915/141479
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1559-0720
info:eu-repo/semantics/altIdentifier/issn/0163-4984
info:eu-repo/semantics/altIdentifier/doi/10.1007/s12011-019-01966-2
info:eu-repo/semantics/altIdentifier/pmid/31705431
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
149-158
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 12.982451

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