Supplementary material from "BvgR is important for virulence-related phenotypes in Bordetella bronchiseptica"

Autores
Gutierrez, María de la Paz; Damron, F. Heath; Sisti, Federico; Fernández, Julieta
Año de publicación
2024
Idioma
español castellano
Tipo de recurso
conjunto de datos
Estado
versión publicada
Descripción
Bordetella bronchiseptica is a pathogenic bacterium that causes respiratory infections in mammals. Adhesins, toxins and secretion systems necessary for infection are regulated by the two-component system BvgAS. When the BvgAS system is inactive, there is no transcription of virulence-activated genes and virulence-repressed genes (vrg) are expressed. The regulation of some vrgs in B. bronchiseptica is dependent upon the virulence-activated gene bvgR. Although having a regulatory role, no DNA-binding domain is described for BvgR. Instead, it contains an EAL domain, usually found in cyclic-di-GMP-specific phosphodiesterases (PDE). Cyclic-di-GMP (c-di-GMP) is a bacterial second messenger that regulates multiple phenotypes in bacteria, including B. bronchiseptica. The current study aimed to deepen our knowledge about BvgR. We employed RNA-seq analysis to define the BvgR regulon and then we investigated the phenotypes in which BvgR regulation might be involved such as biofilm formation, cytotoxicity, and virulence. Our result revealed that BvgR inhibits biofilm formation and flagellin expression in virulent phase. Although BvgR has long been considered a repressor protein, our results show that it also upregulates almost 100 genes. This regulation is likely indirect, as BvgR lacks a DNA-binding domain. Notably, among the upregulated genes, we identified 15 associated with the type three secretion system. Consistent with these findings, a B. bronchiseptica strain deficient in bvgR was less cytotoxic than the wild-type strain, elicited a milder immune response, and was less able to persist in the lower respiratory tract of mice.
Fil: Gutierrez, María de la Paz. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Sisti, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Fernández, Julieta. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Instituto de Biotecnología y Biología Molecular; Argentina
Facultad de Ciencias Exactas
Materia
Ciencias Exactas
Biofilm
Bordetella
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/168456
Acceder
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spelling Supplementary material from "BvgR is important for virulence-related phenotypes in Bordetella bronchiseptica"Gutierrez, María de la PazDamron, F. HeathSisti, FedericoFernández, JulietaCiencias Exactashttps://purl.org/becyt/ford/1.6BiofilmBordetellaBordetella bronchiseptica is a pathogenic bacterium that causes respiratory infections in mammals. Adhesins, toxins and secretion systems necessary for infection are regulated by the two-component system BvgAS. When the BvgAS system is inactive, there is no transcription of virulence-activated genes and virulence-repressed genes (vrg) are expressed. The regulation of some vrgs in B. bronchiseptica is dependent upon the virulence-activated gene bvgR. Although having a regulatory role, no DNA-binding domain is described for BvgR. Instead, it contains an EAL domain, usually found in cyclic-di-GMP-specific phosphodiesterases (PDE). Cyclic-di-GMP (c-di-GMP) is a bacterial second messenger that regulates multiple phenotypes in bacteria, including B. bronchiseptica. The current study aimed to deepen our knowledge about BvgR. We employed RNA-seq analysis to define the BvgR regulon and then we investigated the phenotypes in which BvgR regulation might be involved such as biofilm formation, cytotoxicity, and virulence. Our result revealed that BvgR inhibits biofilm formation and flagellin expression in virulent phase. Although BvgR has long been considered a repressor protein, our results show that it also upregulates almost 100 genes. This regulation is likely indirect, as BvgR lacks a DNA-binding domain. Notably, among the upregulated genes, we identified 15 associated with the type three secretion system. Consistent with these findings, a B. bronchiseptica strain deficient in bvgR was less cytotoxic than the wild-type strain, elicited a milder immune response, and was less able to persist in the lower respiratory tract of mice.Fil: Gutierrez, María de la Paz. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Sisti, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Fernández, Julieta. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Instituto de Biotecnología y Biología Molecular; ArgentinaFacultad de Ciencias Exactas2024-08info:eu-repo/semantics/publishedVersionConjunto de datoshttp://purl.org/coar/resource_type/c_ddb1info:ar-repo/semantics/conjuntoDeDatosinfo:eu-repo/semantics/dataSetapplication/zipEl material es resultado del trabajo citado. Se emplearon técnicas de identificación y cuantificación masiva de proteína (Shot gun proteomics, Orbitrap). Además, se utilizaron técnicas de microbiología clásica: determinación de formación de biofilm, movilidad, etc.http://sedici.unlp.edu.ar/handle/10915/168456https://doi.org/10.35537/10915/168456spainfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:44:51Zoai:sedici.unlp.edu.ar:10915/168456Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:44:52.071SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Supplementary material from "BvgR is important for virulence-related phenotypes in Bordetella bronchiseptica"
title Supplementary material from "BvgR is important for virulence-related phenotypes in Bordetella bronchiseptica"
spellingShingle Supplementary material from "BvgR is important for virulence-related phenotypes in Bordetella bronchiseptica"
Gutierrez, María de la Paz
Ciencias Exactas
Biofilm
Bordetella
title_short Supplementary material from "BvgR is important for virulence-related phenotypes in Bordetella bronchiseptica"
title_full Supplementary material from "BvgR is important for virulence-related phenotypes in Bordetella bronchiseptica"
title_fullStr Supplementary material from "BvgR is important for virulence-related phenotypes in Bordetella bronchiseptica"
title_full_unstemmed Supplementary material from "BvgR is important for virulence-related phenotypes in Bordetella bronchiseptica"
title_sort Supplementary material from "BvgR is important for virulence-related phenotypes in Bordetella bronchiseptica"
dc.creator.none.fl_str_mv Gutierrez, María de la Paz
Damron, F. Heath
Sisti, Federico
Fernández, Julieta
author Gutierrez, María de la Paz
author_facet Gutierrez, María de la Paz
Damron, F. Heath
Sisti, Federico
Fernández, Julieta
author_role author
author2 Damron, F. Heath
Sisti, Federico
Fernández, Julieta
author2_role author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Biofilm
Bordetella
topic Ciencias Exactas
Biofilm
Bordetella
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
dc.description.none.fl_txt_mv Bordetella bronchiseptica is a pathogenic bacterium that causes respiratory infections in mammals. Adhesins, toxins and secretion systems necessary for infection are regulated by the two-component system BvgAS. When the BvgAS system is inactive, there is no transcription of virulence-activated genes and virulence-repressed genes (vrg) are expressed. The regulation of some vrgs in B. bronchiseptica is dependent upon the virulence-activated gene bvgR. Although having a regulatory role, no DNA-binding domain is described for BvgR. Instead, it contains an EAL domain, usually found in cyclic-di-GMP-specific phosphodiesterases (PDE). Cyclic-di-GMP (c-di-GMP) is a bacterial second messenger that regulates multiple phenotypes in bacteria, including B. bronchiseptica. The current study aimed to deepen our knowledge about BvgR. We employed RNA-seq analysis to define the BvgR regulon and then we investigated the phenotypes in which BvgR regulation might be involved such as biofilm formation, cytotoxicity, and virulence. Our result revealed that BvgR inhibits biofilm formation and flagellin expression in virulent phase. Although BvgR has long been considered a repressor protein, our results show that it also upregulates almost 100 genes. This regulation is likely indirect, as BvgR lacks a DNA-binding domain. Notably, among the upregulated genes, we identified 15 associated with the type three secretion system. Consistent with these findings, a B. bronchiseptica strain deficient in bvgR was less cytotoxic than the wild-type strain, elicited a milder immune response, and was less able to persist in the lower respiratory tract of mice.
Fil: Gutierrez, María de la Paz. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Sisti, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Fernández, Julieta. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Instituto de Biotecnología y Biología Molecular; Argentina
Facultad de Ciencias Exactas
description Bordetella bronchiseptica is a pathogenic bacterium that causes respiratory infections in mammals. Adhesins, toxins and secretion systems necessary for infection are regulated by the two-component system BvgAS. When the BvgAS system is inactive, there is no transcription of virulence-activated genes and virulence-repressed genes (vrg) are expressed. The regulation of some vrgs in B. bronchiseptica is dependent upon the virulence-activated gene bvgR. Although having a regulatory role, no DNA-binding domain is described for BvgR. Instead, it contains an EAL domain, usually found in cyclic-di-GMP-specific phosphodiesterases (PDE). Cyclic-di-GMP (c-di-GMP) is a bacterial second messenger that regulates multiple phenotypes in bacteria, including B. bronchiseptica. The current study aimed to deepen our knowledge about BvgR. We employed RNA-seq analysis to define the BvgR regulon and then we investigated the phenotypes in which BvgR regulation might be involved such as biofilm formation, cytotoxicity, and virulence. Our result revealed that BvgR inhibits biofilm formation and flagellin expression in virulent phase. Although BvgR has long been considered a repressor protein, our results show that it also upregulates almost 100 genes. This regulation is likely indirect, as BvgR lacks a DNA-binding domain. Notably, among the upregulated genes, we identified 15 associated with the type three secretion system. Consistent with these findings, a B. bronchiseptica strain deficient in bvgR was less cytotoxic than the wild-type strain, elicited a milder immune response, and was less able to persist in the lower respiratory tract of mice.
publishDate 2024
dc.date.none.fl_str_mv 2024-08
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
Conjunto de datos
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info:ar-repo/semantics/conjuntoDeDatos
info:eu-repo/semantics/dataSet
status_str publishedVersion
format dataSet
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/168456
https://doi.org/10.35537/10915/168456
url http://sedici.unlp.edu.ar/handle/10915/168456
https://doi.org/10.35537/10915/168456
dc.language.none.fl_str_mv spa
language spa
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/zip
El material es resultado del trabajo citado. Se emplearon técnicas de identificación y cuantificación masiva de proteína (Shot gun proteomics, Orbitrap). Además, se utilizaron técnicas de microbiología clásica: determinación de formación de biofilm, movilidad, etc.
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instname_str Universidad Nacional de La Plata
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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