Emulsan-Alginate Microspheres as a New Vehicle for Protein Delivery

Autores
Castro, Guillermo Raúl; Bora, Emilia; Panilaitis, Bruce; Kaplan, David I.; Khemani, Kishan C.; Scholz, Carmen
Año de publicación
2006
Idioma
inglés
Tipo de recurso
parte de libro
Estado
versión publicada
Descripción
A solution containing emulsan, a lipoheteropolysaccharide, and calcium was used to produce emulsan-alginate microspheres (EAMs). Optical, scanning electron microscopy and EDX (Energy Dispersive X-ray) analysis of the microspheres suggested different morphologies and compositions, respectively, when compared with microspheres prepared only from alginate. The EAMs were twice as stable in phosphate solution compared to alginate alone when assessed with blue dextran encapsulation. The EAMs were able to adsorb about twice the amount of BSA (Bovine Serum Albumin) compared to alginate alone. When azo-BSA was adsorbed on the emulsan-alginate microspheres, protein release could be triggered with enzymes. BSA released from the EAMs retained about of 78% of the -helix structure.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Laboratorio de Nanobiomateriales
Materia
Química
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/130967
Acceder
id SEDICI_cb76b1e91607bc9d6470ebab0049f3c3
oai_identifier_str oai:sedici.unlp.edu.ar:10915/130967
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Emulsan-Alginate Microspheres as a New Vehicle for Protein DeliveryCastro, Guillermo RaúlBora, EmiliaPanilaitis, BruceKaplan, David I.Khemani, Kishan C.Scholz, CarmenQuímicaA solution containing emulsan, a lipoheteropolysaccharide, and calcium was used to produce emulsan-alginate microspheres (EAMs). Optical, scanning electron microscopy and EDX (Energy Dispersive X-ray) analysis of the microspheres suggested different morphologies and compositions, respectively, when compared with microspheres prepared only from alginate. The EAMs were twice as stable in phosphate solution compared to alginate alone when assessed with blue dextran encapsulation. The EAMs were able to adsorb about twice the amount of BSA (Bovine Serum Albumin) compared to alginate alone. When azo-BSA was adsorbed on the emulsan-alginate microspheres, protein release could be triggered with enzymes. BSA released from the EAMs retained about of 78% of the -helix structure.Centro de Investigación y Desarrollo en Fermentaciones IndustrialesLaboratorio de NanobiomaterialesAmerican Chemical Society2006info:eu-repo/semantics/bookPartinfo:eu-repo/semantics/publishedVersionCapitulo de librohttp://purl.org/coar/resource_type/c_3248info:ar-repo/semantics/parteDeLibroapplication/pdf14-29http://sedici.unlp.edu.ar/handle/10915/130967enginfo:eu-repo/semantics/altIdentifier/isbn/978-084-12-3972-2info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-11-05T13:12:17Zoai:sedici.unlp.edu.ar:10915/130967Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-11-05 13:12:17.94SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Emulsan-Alginate Microspheres as a New Vehicle for Protein Delivery
title Emulsan-Alginate Microspheres as a New Vehicle for Protein Delivery
spellingShingle Emulsan-Alginate Microspheres as a New Vehicle for Protein Delivery
Castro, Guillermo Raúl
Química
title_short Emulsan-Alginate Microspheres as a New Vehicle for Protein Delivery
title_full Emulsan-Alginate Microspheres as a New Vehicle for Protein Delivery
title_fullStr Emulsan-Alginate Microspheres as a New Vehicle for Protein Delivery
title_full_unstemmed Emulsan-Alginate Microspheres as a New Vehicle for Protein Delivery
title_sort Emulsan-Alginate Microspheres as a New Vehicle for Protein Delivery
dc.creator.none.fl_str_mv Castro, Guillermo Raúl
Bora, Emilia
Panilaitis, Bruce
Kaplan, David I.
Khemani, Kishan C.
Scholz, Carmen
author Castro, Guillermo Raúl
author_facet Castro, Guillermo Raúl
Bora, Emilia
Panilaitis, Bruce
Kaplan, David I.
Khemani, Kishan C.
Scholz, Carmen
author_role author
author2 Bora, Emilia
Panilaitis, Bruce
Kaplan, David I.
Khemani, Kishan C.
Scholz, Carmen
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Química
topic Química
dc.description.none.fl_txt_mv A solution containing emulsan, a lipoheteropolysaccharide, and calcium was used to produce emulsan-alginate microspheres (EAMs). Optical, scanning electron microscopy and EDX (Energy Dispersive X-ray) analysis of the microspheres suggested different morphologies and compositions, respectively, when compared with microspheres prepared only from alginate. The EAMs were twice as stable in phosphate solution compared to alginate alone when assessed with blue dextran encapsulation. The EAMs were able to adsorb about twice the amount of BSA (Bovine Serum Albumin) compared to alginate alone. When azo-BSA was adsorbed on the emulsan-alginate microspheres, protein release could be triggered with enzymes. BSA released from the EAMs retained about of 78% of the -helix structure.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Laboratorio de Nanobiomateriales
description A solution containing emulsan, a lipoheteropolysaccharide, and calcium was used to produce emulsan-alginate microspheres (EAMs). Optical, scanning electron microscopy and EDX (Energy Dispersive X-ray) analysis of the microspheres suggested different morphologies and compositions, respectively, when compared with microspheres prepared only from alginate. The EAMs were twice as stable in phosphate solution compared to alginate alone when assessed with blue dextran encapsulation. The EAMs were able to adsorb about twice the amount of BSA (Bovine Serum Albumin) compared to alginate alone. When azo-BSA was adsorbed on the emulsan-alginate microspheres, protein release could be triggered with enzymes. BSA released from the EAMs retained about of 78% of the -helix structure.
publishDate 2006
dc.date.none.fl_str_mv 2006
dc.type.none.fl_str_mv info:eu-repo/semantics/bookPart
info:eu-repo/semantics/publishedVersion
Capitulo de libro
http://purl.org/coar/resource_type/c_3248
info:ar-repo/semantics/parteDeLibro
format bookPart
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/130967
url http://sedici.unlp.edu.ar/handle/10915/130967
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/isbn/978-084-12-3972-2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
14-29
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
_version_ 1847978768465920000
score 13.087074

Publicaciones similares