Fructose rich diet-induced high plasminogen activator inhibitor-1 (PAI-1) production in the adult female rat: Protective effect of progesterone

Autores
Castrogiovanni, Daniel; Alzamendi, Ana; Ongaro Gambino, Luisina; Giovambattista, Andrés; Gaillard, Rolf C.; Spinedi, Eduardo
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effect of progesterone (P4) on fructose rich diet (FRD) intake-induced metabolic, endocrine and parametrial adipose tissue (PMAT) dysfunctions was studied in the adult female rat. Sixty day-old rats were i.m. treated with oil alone (control, CT) or containing P4 (12 mg/kg). Rats ate Purina chow-diet ad libitum throughout the entire experiment and, between 100 and 120 days of age drank ad libitum tap water alone (normal diet; CT-ND and P4-ND) or containing fructose (10% w/v; CT-FRD and P4-FRD). At age 120 days, animals were subjected to a glucose tolerance test or decapitated. Plasma concentrations of various biomarkers and PMAT gene abundance were monitored. P4-ND (vs. CT-ND) rats showed elevated circulating levels of lipids. CT-FRD rats displayed high (vs. CT-ND) plasma concentrations of lipids, leptin, adiponectin and plasminogen activator inhibitor-1 (PAI-1). Lipidemia and adiponectinemia were high (vs. P4-ND) in P4-FRD rats. Although P4 failed to prevent FRD-induced hyperleptinemia, it was fully protective on FRD-enhanced plasma PAI-1 levels. PMAT leptin and adiponectin mRNAs were high in CT-FRD and P4-FRD rats. While FRD enhanced PMAT PAI-1 mRNA abundance in CT rats, this effect was absent in P4 rats. Our study supports that a preceding P4-enriched milieu prevented the enhanced prothrombotic risk induced by FRD-elicited high PAI-1 production.
Instituto Multidisciplinario de Biología Celular
Facultad de Ciencias Médicas
Facultad de Ciencias Exactas
Materia
Ciencias Exactas
Adipokines
Allostasis
Glucose tolerance
High carbohydrate diet
Insulin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/83633
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/83633
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Fructose rich diet-induced high plasminogen activator inhibitor-1 (PAI-1) production in the adult female rat: Protective effect of progesteroneCastrogiovanni, DanielAlzamendi, AnaOngaro Gambino, LuisinaGiovambattista, AndrésGaillard, Rolf C.Spinedi, EduardoCiencias ExactasAdipokinesAllostasisGlucose toleranceHigh carbohydrate dietInsulinThe effect of progesterone (P4) on fructose rich diet (FRD) intake-induced metabolic, endocrine and parametrial adipose tissue (PMAT) dysfunctions was studied in the adult female rat. Sixty day-old rats were i.m. treated with oil alone (control, CT) or containing P4 (12 mg/kg). Rats ate Purina chow-diet <i>ad libitum</i> throughout the entire experiment and, between 100 and 120 days of age drank <i>ad libitum</i> tap water alone (normal diet; CT-ND and P4-ND) or containing fructose (10% w/v; CT-FRD and P4-FRD). At age 120 days, animals were subjected to a glucose tolerance test or decapitated. Plasma concentrations of various biomarkers and PMAT gene abundance were monitored. P4-ND (vs. CT-ND) rats showed elevated circulating levels of lipids. CT-FRD rats displayed high (vs. CT-ND) plasma concentrations of lipids, leptin, adiponectin and plasminogen activator inhibitor-1 (PAI-1). Lipidemia and adiponectinemia were high (vs. P4-ND) in P4-FRD rats. Although P4 failed to prevent FRD-induced hyperleptinemia, it was fully protective on FRD-enhanced plasma PAI-1 levels. PMAT leptin and adiponectin mRNAs were high in CT-FRD and P4-FRD rats. While FRD enhanced PMAT PAI-1 mRNA abundance in CT rats, this effect was absent in P4 rats. Our study supports that a preceding P4-enriched milieu prevented the enhanced prothrombotic risk induced by FRD-elicited high PAI-1 production.Instituto Multidisciplinario de Biología CelularFacultad de Ciencias MédicasFacultad de Ciencias Exactas2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1137-1150http://sedici.unlp.edu.ar/handle/10915/83633enginfo:eu-repo/semantics/altIdentifier/issn/2072-6643info:eu-repo/semantics/altIdentifier/doi/10.3390/nu4081137info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:15:55Zoai:sedici.unlp.edu.ar:10915/83633Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:15:55.85SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Fructose rich diet-induced high plasminogen activator inhibitor-1 (PAI-1) production in the adult female rat: Protective effect of progesterone
title Fructose rich diet-induced high plasminogen activator inhibitor-1 (PAI-1) production in the adult female rat: Protective effect of progesterone
spellingShingle Fructose rich diet-induced high plasminogen activator inhibitor-1 (PAI-1) production in the adult female rat: Protective effect of progesterone
Castrogiovanni, Daniel
Ciencias Exactas
Adipokines
Allostasis
Glucose tolerance
High carbohydrate diet
Insulin
title_short Fructose rich diet-induced high plasminogen activator inhibitor-1 (PAI-1) production in the adult female rat: Protective effect of progesterone
title_full Fructose rich diet-induced high plasminogen activator inhibitor-1 (PAI-1) production in the adult female rat: Protective effect of progesterone
title_fullStr Fructose rich diet-induced high plasminogen activator inhibitor-1 (PAI-1) production in the adult female rat: Protective effect of progesterone
title_full_unstemmed Fructose rich diet-induced high plasminogen activator inhibitor-1 (PAI-1) production in the adult female rat: Protective effect of progesterone
title_sort Fructose rich diet-induced high plasminogen activator inhibitor-1 (PAI-1) production in the adult female rat: Protective effect of progesterone
dc.creator.none.fl_str_mv Castrogiovanni, Daniel
Alzamendi, Ana
Ongaro Gambino, Luisina
Giovambattista, Andrés
Gaillard, Rolf C.
Spinedi, Eduardo
author Castrogiovanni, Daniel
author_facet Castrogiovanni, Daniel
Alzamendi, Ana
Ongaro Gambino, Luisina
Giovambattista, Andrés
Gaillard, Rolf C.
Spinedi, Eduardo
author_role author
author2 Alzamendi, Ana
Ongaro Gambino, Luisina
Giovambattista, Andrés
Gaillard, Rolf C.
Spinedi, Eduardo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Adipokines
Allostasis
Glucose tolerance
High carbohydrate diet
Insulin
topic Ciencias Exactas
Adipokines
Allostasis
Glucose tolerance
High carbohydrate diet
Insulin
dc.description.none.fl_txt_mv The effect of progesterone (P4) on fructose rich diet (FRD) intake-induced metabolic, endocrine and parametrial adipose tissue (PMAT) dysfunctions was studied in the adult female rat. Sixty day-old rats were i.m. treated with oil alone (control, CT) or containing P4 (12 mg/kg). Rats ate Purina chow-diet <i>ad libitum</i> throughout the entire experiment and, between 100 and 120 days of age drank <i>ad libitum</i> tap water alone (normal diet; CT-ND and P4-ND) or containing fructose (10% w/v; CT-FRD and P4-FRD). At age 120 days, animals were subjected to a glucose tolerance test or decapitated. Plasma concentrations of various biomarkers and PMAT gene abundance were monitored. P4-ND (vs. CT-ND) rats showed elevated circulating levels of lipids. CT-FRD rats displayed high (vs. CT-ND) plasma concentrations of lipids, leptin, adiponectin and plasminogen activator inhibitor-1 (PAI-1). Lipidemia and adiponectinemia were high (vs. P4-ND) in P4-FRD rats. Although P4 failed to prevent FRD-induced hyperleptinemia, it was fully protective on FRD-enhanced plasma PAI-1 levels. PMAT leptin and adiponectin mRNAs were high in CT-FRD and P4-FRD rats. While FRD enhanced PMAT PAI-1 mRNA abundance in CT rats, this effect was absent in P4 rats. Our study supports that a preceding P4-enriched milieu prevented the enhanced prothrombotic risk induced by FRD-elicited high PAI-1 production.
Instituto Multidisciplinario de Biología Celular
Facultad de Ciencias Médicas
Facultad de Ciencias Exactas
description The effect of progesterone (P4) on fructose rich diet (FRD) intake-induced metabolic, endocrine and parametrial adipose tissue (PMAT) dysfunctions was studied in the adult female rat. Sixty day-old rats were i.m. treated with oil alone (control, CT) or containing P4 (12 mg/kg). Rats ate Purina chow-diet <i>ad libitum</i> throughout the entire experiment and, between 100 and 120 days of age drank <i>ad libitum</i> tap water alone (normal diet; CT-ND and P4-ND) or containing fructose (10% w/v; CT-FRD and P4-FRD). At age 120 days, animals were subjected to a glucose tolerance test or decapitated. Plasma concentrations of various biomarkers and PMAT gene abundance were monitored. P4-ND (vs. CT-ND) rats showed elevated circulating levels of lipids. CT-FRD rats displayed high (vs. CT-ND) plasma concentrations of lipids, leptin, adiponectin and plasminogen activator inhibitor-1 (PAI-1). Lipidemia and adiponectinemia were high (vs. P4-ND) in P4-FRD rats. Although P4 failed to prevent FRD-induced hyperleptinemia, it was fully protective on FRD-enhanced plasma PAI-1 levels. PMAT leptin and adiponectin mRNAs were high in CT-FRD and P4-FRD rats. While FRD enhanced PMAT PAI-1 mRNA abundance in CT rats, this effect was absent in P4 rats. Our study supports that a preceding P4-enriched milieu prevented the enhanced prothrombotic risk induced by FRD-elicited high PAI-1 production.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/83633
url http://sedici.unlp.edu.ar/handle/10915/83633
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2072-6643
info:eu-repo/semantics/altIdentifier/doi/10.3390/nu4081137
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
1137-1150
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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