Evidence supporting a role for the blood-cerebrospinal fluid barrier transporting circulating ghrelin into the brain
- Autores
- Uriarte Donati, Maia; De Francesco, Pablo Nicolás; Fernández, Gimena; Cabral, Agustina Soledad; Castrogiovanni, Daniel Cayetano; Lalonde, Tyler; Luyt, Leonard G.; Trejo, Sebastián Alejandro; Perelló, Mario Carlos
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The stomach-derived hormone ghrelin mainly acts in the brain. Studies in mice have shown that the accessibility of ghrelin into the brain is limited and that it mainly takes place in some circumventricular organs, such as the median eminence. Notably, some known brain targets of ghrelin are distantly located from the circumventricular organs. Thus, we hypothesized that ghrelin could also access the brain via the blood-cerebrospinal fluid (CSF) barrier, which consists of the choroid plexus and the hypothalamic tanycytes. Using systemic injection of ghrelin or fluorescent-ghrelin in mice, we found that cells of the blood-CSF barrier internalize these molecules. In time-response studies, we found that peripherally injected fluorescent-ghrelin quickly reaches hypothalamic regions located in apposition to the median eminence and more slowly reaches the periventricular hypothalamic parenchyma, adjacent to the dorsal part of the third ventricle. Additionally, we found that CSF ghrelin levels increase after the systemic administration of ghrelin, and that central infusions of either an anti-ghrelin antibody, which immuno-neutralizes CSF ghrelin, or a scrambled version of ghrelin, which is also internalized by cells of the blood-CSF barrier, partially impair the orexigenic effect of peripherally injected ghrelin. Thus, current evidence suggests that the blood-CSF barrier can transport circulating ghrelin into the brain, and that the access of ghrelin into the CSF is required for its full orexigenic effect.
Instituto Multidisciplinario de Biología Celular - Materia
-
Biología
Ependymal cells
Choroid plexus
Tanycytes
Hypothalamus
Ghrelin - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/145491
Ver los metadatos del registro completo
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Evidence supporting a role for the blood-cerebrospinal fluid barrier transporting circulating ghrelin into the brainUriarte Donati, MaiaDe Francesco, Pablo NicolásFernández, GimenaCabral, Agustina SoledadCastrogiovanni, Daniel CayetanoLalonde, TylerLuyt, Leonard G.Trejo, Sebastián AlejandroPerelló, Mario CarlosBiologíaEpendymal cellsChoroid plexusTanycytesHypothalamusGhrelinThe stomach-derived hormone ghrelin mainly acts in the brain. Studies in mice have shown that the accessibility of ghrelin into the brain is limited and that it mainly takes place in some circumventricular organs, such as the median eminence. Notably, some known brain targets of ghrelin are distantly located from the circumventricular organs. Thus, we hypothesized that ghrelin could also access the brain via the blood-cerebrospinal fluid (CSF) barrier, which consists of the choroid plexus and the hypothalamic tanycytes. Using systemic injection of ghrelin or fluorescent-ghrelin in mice, we found that cells of the blood-CSF barrier internalize these molecules. In time-response studies, we found that peripherally injected fluorescent-ghrelin quickly reaches hypothalamic regions located in apposition to the median eminence and more slowly reaches the periventricular hypothalamic parenchyma, adjacent to the dorsal part of the third ventricle. Additionally, we found that CSF ghrelin levels increase after the systemic administration of ghrelin, and that central infusions of either an anti-ghrelin antibody, which immuno-neutralizes CSF ghrelin, or a scrambled version of ghrelin, which is also internalized by cells of the blood-CSF barrier, partially impair the orexigenic effect of peripherally injected ghrelin. Thus, current evidence suggests that the blood-CSF barrier can transport circulating ghrelin into the brain, and that the access of ghrelin into the CSF is required for its full orexigenic effect.Instituto Multidisciplinario de Biología Celular2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf4120-4134http://sedici.unlp.edu.ar/handle/10915/145491enginfo:eu-repo/semantics/altIdentifier/issn/1559-1182info:eu-repo/semantics/altIdentifier/issn/0893-7648info:eu-repo/semantics/altIdentifier/doi/10.1007/s12035-018-1362-8info:eu-repo/semantics/altIdentifier/pmid/30276663info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:27Zoai:sedici.unlp.edu.ar:10915/145491Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:28.216SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Evidence supporting a role for the blood-cerebrospinal fluid barrier transporting circulating ghrelin into the brain |
title |
Evidence supporting a role for the blood-cerebrospinal fluid barrier transporting circulating ghrelin into the brain |
spellingShingle |
Evidence supporting a role for the blood-cerebrospinal fluid barrier transporting circulating ghrelin into the brain Uriarte Donati, Maia Biología Ependymal cells Choroid plexus Tanycytes Hypothalamus Ghrelin |
title_short |
Evidence supporting a role for the blood-cerebrospinal fluid barrier transporting circulating ghrelin into the brain |
title_full |
Evidence supporting a role for the blood-cerebrospinal fluid barrier transporting circulating ghrelin into the brain |
title_fullStr |
Evidence supporting a role for the blood-cerebrospinal fluid barrier transporting circulating ghrelin into the brain |
title_full_unstemmed |
Evidence supporting a role for the blood-cerebrospinal fluid barrier transporting circulating ghrelin into the brain |
title_sort |
Evidence supporting a role for the blood-cerebrospinal fluid barrier transporting circulating ghrelin into the brain |
dc.creator.none.fl_str_mv |
Uriarte Donati, Maia De Francesco, Pablo Nicolás Fernández, Gimena Cabral, Agustina Soledad Castrogiovanni, Daniel Cayetano Lalonde, Tyler Luyt, Leonard G. Trejo, Sebastián Alejandro Perelló, Mario Carlos |
author |
Uriarte Donati, Maia |
author_facet |
Uriarte Donati, Maia De Francesco, Pablo Nicolás Fernández, Gimena Cabral, Agustina Soledad Castrogiovanni, Daniel Cayetano Lalonde, Tyler Luyt, Leonard G. Trejo, Sebastián Alejandro Perelló, Mario Carlos |
author_role |
author |
author2 |
De Francesco, Pablo Nicolás Fernández, Gimena Cabral, Agustina Soledad Castrogiovanni, Daniel Cayetano Lalonde, Tyler Luyt, Leonard G. Trejo, Sebastián Alejandro Perelló, Mario Carlos |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
Biología Ependymal cells Choroid plexus Tanycytes Hypothalamus Ghrelin |
topic |
Biología Ependymal cells Choroid plexus Tanycytes Hypothalamus Ghrelin |
dc.description.none.fl_txt_mv |
The stomach-derived hormone ghrelin mainly acts in the brain. Studies in mice have shown that the accessibility of ghrelin into the brain is limited and that it mainly takes place in some circumventricular organs, such as the median eminence. Notably, some known brain targets of ghrelin are distantly located from the circumventricular organs. Thus, we hypothesized that ghrelin could also access the brain via the blood-cerebrospinal fluid (CSF) barrier, which consists of the choroid plexus and the hypothalamic tanycytes. Using systemic injection of ghrelin or fluorescent-ghrelin in mice, we found that cells of the blood-CSF barrier internalize these molecules. In time-response studies, we found that peripherally injected fluorescent-ghrelin quickly reaches hypothalamic regions located in apposition to the median eminence and more slowly reaches the periventricular hypothalamic parenchyma, adjacent to the dorsal part of the third ventricle. Additionally, we found that CSF ghrelin levels increase after the systemic administration of ghrelin, and that central infusions of either an anti-ghrelin antibody, which immuno-neutralizes CSF ghrelin, or a scrambled version of ghrelin, which is also internalized by cells of the blood-CSF barrier, partially impair the orexigenic effect of peripherally injected ghrelin. Thus, current evidence suggests that the blood-CSF barrier can transport circulating ghrelin into the brain, and that the access of ghrelin into the CSF is required for its full orexigenic effect. Instituto Multidisciplinario de Biología Celular |
description |
The stomach-derived hormone ghrelin mainly acts in the brain. Studies in mice have shown that the accessibility of ghrelin into the brain is limited and that it mainly takes place in some circumventricular organs, such as the median eminence. Notably, some known brain targets of ghrelin are distantly located from the circumventricular organs. Thus, we hypothesized that ghrelin could also access the brain via the blood-cerebrospinal fluid (CSF) barrier, which consists of the choroid plexus and the hypothalamic tanycytes. Using systemic injection of ghrelin or fluorescent-ghrelin in mice, we found that cells of the blood-CSF barrier internalize these molecules. In time-response studies, we found that peripherally injected fluorescent-ghrelin quickly reaches hypothalamic regions located in apposition to the median eminence and more slowly reaches the periventricular hypothalamic parenchyma, adjacent to the dorsal part of the third ventricle. Additionally, we found that CSF ghrelin levels increase after the systemic administration of ghrelin, and that central infusions of either an anti-ghrelin antibody, which immuno-neutralizes CSF ghrelin, or a scrambled version of ghrelin, which is also internalized by cells of the blood-CSF barrier, partially impair the orexigenic effect of peripherally injected ghrelin. Thus, current evidence suggests that the blood-CSF barrier can transport circulating ghrelin into the brain, and that the access of ghrelin into the CSF is required for its full orexigenic effect. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/145491 |
url |
http://sedici.unlp.edu.ar/handle/10915/145491 |
dc.language.none.fl_str_mv |
eng |
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eng |
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openAccess |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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